Avaliação da produção de mediadores inflamatórios por macrófagos murinos estimulados com Synoeca-MP e levofloxacina na presença de cepas de Klebsiella pneumoniae

• Ano de defesa: 2019
• Autor(a) principal: Ferreira, Arthur Corrêa Resende
• Orientador(a): Dias, Simoni Campos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Católica de Brasília
• Programa de Pós-Graduação: Programa Stricto Sensu em Ciências Genômicas e Biotecnologia
• Departamento: Escola de Saúde e Medicina
• País: Brasil
• Palavras-chave em Português: Synoeca-MP; Sistema imune; Klebsiella pneumoniae; Peptídeo antimicrobiano; Mediadores inflamatórios
• Palavras-chave em Inglês: Antimicrobial peptide; Immune system
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS
• Resumo em Inglês: Klebsiella pneumoniae is a microorganism of great relevance in clinical context because it presents resistance to several antibiotics, besides possessing the ability to evade the immune system of the host. Antimicrobial therapy has encountered several barriers mainly due to the emergence of multiresistant strains, which reinforces the need to formulate new antimicrobial and adjuvant agents. Within this scenario, immunomodulatory peptides may be an alternative for the protection of the host immune system in this type of infection. Therefore, the objective of this work was to evaluate the immunomodulatory potential of the Synoeca-MP peptide against an in vitro model of K. pneumoniae, evaluating inflammatory mediators. Knowing that K. pneumoniae strains that present capsules have greater virulence and respond differently to treatments, the presence of this capsule was initially evaluated in the strains of K. pneumoniae (Kp2177569 and ATCC 13883) used in this study. Subsequently, cell viability, nitric oxide production and inflammatory mediators, such as tumor necrosis factor alpha and interleukin 10, were evaluated in RAW 264.7 cells, incubated with heat-inactivated K. pneumoniae antigens, Synoeca-MP and levofloxacin and the combination of both. In the presence of the antigens, the viability was maintained and the production of nitric oxide increased when compared to the control. It was observed that the presence of Synoeca-MP and levofloxacin did not alter the production of nitric oxide. However, when this association was incubated with the cells stimulated in the presence of K. pneumoniae antigen ATCC 13883 it was observed that TNF-α levels were maintained, in addition to a reduction in IL-10 production. In this way, from the obtained results, the association is able to present a low modulating action of pro and anti-inflammatory mediators.
• Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/2606

Klebsiella pneumoniae is a microorganism of great relevance in clinical context because it presents resistance to several antibiotics, besides possessing the ability to evade the immune system of the host. Antimicrobial therapy has encountered several barriers mainly due to the emergence of multiresistant strains, which reinforces the need to formulate new antimicrobial and adjuvant agents. Within this scenario, immunomodulatory peptides may be an alternative for the protection of the host immune system in this type of infection. Therefore, the objective of this work was to evaluate the immunomodulatory potential of the Synoeca-MP peptide against an in vitro model of K. pneumoniae, evaluating inflammatory mediators. Knowing that K. pneumoniae strains that present capsules have greater virulence and respond differently to treatments, the presence of this capsule was initially evaluated in the strains of K. pneumoniae (Kp2177569 and ATCC 13883) used in this study. Subsequently, cell viability, nitric oxide production and inflammatory mediators, such as tumor necrosis factor alpha and interleukin 10, were evaluated in RAW 264.7 cells, incubated with heat-inactivated K. pneumoniae antigens, Synoeca-MP and levofloxacin and the combination of both. In the presence of the antigens, the viability was maintained and the production of nitric oxide increased when compared to the control. It was observed that the presence of Synoeca-MP and levofloxacin did not alter the production of nitric oxide. However, when this association was incubated with the cells stimulated in the presence of K. pneumoniae antigen ATCC 13883 it was observed that TNF-α levels were maintained, in addition to a reduction in IL-10 production. In this way, from the obtained results, the association is able to present a low modulating action of pro and anti-inflammatory mediators.