Avaliação dos efeitos da biocerâmica na inflamação periférica em camundongos : análise do mecanismo de ação

• Ano de defesa: 2017
• Autor(a) principal: Rosas, Ralph Fernando
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Sistemas endógenos; Inflamação periférica; Biocerâmica; CFA; Adjuvante completo de Freund; Hiperalgesia
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/3155

Introduction: Bioceramics have been studied for their ability to emit long infrared radiation producing non-thermal and thermal effects, with several biological activities. Objective: To evaluate the effects of bioceramics on peripheral inflammation in mice, as well as to analyze the possible neurobiological mechanism involved in this effect. Methods: Swiss male mice injected with CFA in the paw were submitted to bioceramic treatments by means of contact with a ceramic impregnated pad that, under paw heat, radiates far infrared. Six groups (n = 8) of animals were randomly distributed into naive, control group and treated by exposure (radiation) to the bioceramics for 30, 60, 120 minutes or by continuous exposure. Edema formation, Mechanical and thermal hyperalgesia were analyzed using behavioral tests, i.e., the von Frey test, a hot plate and a micrometer (paw thickness), respectively. In the biochemical analyzes the local tissue (paw) concentrations of pro and anti-inflammatory cytokines were measured by the ELISA method. In the oxidative stress analyzes, parameters related to oxidative damage (TBARS and carbonylated proteins), antioxidant defense (SOD and CAT) and inflammation (myeloperoxidase and nitrite / nitrate concentrations) were analyzed by spectrophotometry. Intraplantar administrations of AM281 or AM630 (cannabinoid), caffeine and DPCPX (adenosine), naloxone (opioid) were used to investigate the endogenous pain control systems involved in the antihyperalgesic activity of bioceramics. Results: Bioceramic treatment reduced edema, as well as mechanical and thermal hyperalgesia. In addition, it decreased TNF-α and IL-1β concentrations and increased IL-10. The treatment also decreased oxidative damage and increased the activity of antioxidant enzymes. Activation of μ-opioid receptors, A1, CB1 and CB2 appear to mediate the antihyperalgesic effect of bioceramics. Conclusion: Material impregnated with bioceramics has anti-hyperalgesic and antiedematogenic activity in vivo. This antihyperalgesic effect seems to be related to its antioxidant and anti-inflammatory activities, as well as its ability to activate endogenous pain control systems.