Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Pires, Juliana de Almeida lattes
Orientador(a): Silva Junior, Jose Antonio lattes
Banca de defesa: Silva Junior, Jose Antonio lattes, Silva, Carlos Alberto lattes, Serra, Andrey Jorge lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Nove de Julho
Programa de Pós-Graduação: Programa de Mestrado em Medicina
Departamento: Saúde
País: Brasil
Palavras-chave em Português:
apoptose
CASPASE 3
BCL-2
BAX
infarto do miocárdio
laser de baixa intensidade
Palavras-chave em Inglês:
apoptosis
CASPASE 3
BCL-2
BAX
myocardial infarction
low level laser therapy
Área do conhecimento CNPq:
CIENCIAS DA SAUDE
Link de acesso: http://bibliotecatede.uninove.br/handle/tede/3318
Resumo: Members of the Bcl -2 family are important apoptotic regulators. The Bcl -2 protein can act by activating or repressing apoptosis and may contribute to the emergence of diseases or protect the heart from various heart diseases, respectively. Caspase -3 is an effector protease which cleavage promotes changes that result in cell death. Modulation of apoptotic factors, generated by coronary occlusion and subsequent cell death, after the application of low intensity laser is little studied yet. Using an experimental model, 15 Wistar rats (200-280g) were subjected to myocardial infarction by coronary artery occlusion and randomized into three groups: control (control rats); IM (infarcted rats); IM + Laser (infarcted rats and irradiated with laser). In IM + Laser Group, the infarcted area was treated with laser (λ 660 nm, 15 mW, 60 seconds, irradiated area 0.785 cm2, energy density 22,5 J/cm2 and energy delivered 1,1 Joules) after coronary occlusion. The messenger RNAs of Bax and Bcl2 were quantified by PCR - Real Time (Sybr Green). The Western blotting procedure was conducted to evaluate the protein expression of Bax, Bcl2 and caspase 3. Was observed an increase in gene expression and protein of Bcl2 in IM + Laser group when compared to the IM group (p≤0,05), however no significant difference in gene expression of Bax was found. There was a decrease in the expression of cleaved caspase3 the IM group + Laser in relation to the infarcted group. Our results support the hypothesis that low level lasertherapy can stimulate cell survival after a heart attack, possibly by reducing apoptosis of viable area of the heart to maintain cardiac homeostasis after infarction
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spelling Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161Silva, Carlos Albertohttp://lattes.cnpq.br/5754147808501113Serra, Andrey Jorgehttp://lattes.cnpq.br/6499058447489317http://lattes.cnpq.br/5180416080487653Pires, Juliana de Almeida2024-04-08T21:29:10Z2016-08-03Pires, Juliana de Almeida. Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos. 2016. 73 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/3318Members of the Bcl -2 family are important apoptotic regulators. The Bcl -2 protein can act by activating or repressing apoptosis and may contribute to the emergence of diseases or protect the heart from various heart diseases, respectively. Caspase -3 is an effector protease which cleavage promotes changes that result in cell death. Modulation of apoptotic factors, generated by coronary occlusion and subsequent cell death, after the application of low intensity laser is little studied yet. Using an experimental model, 15 Wistar rats (200-280g) were subjected to myocardial infarction by coronary artery occlusion and randomized into three groups: control (control rats); IM (infarcted rats); IM + Laser (infarcted rats and irradiated with laser). In IM + Laser Group, the infarcted area was treated with laser (λ 660 nm, 15 mW, 60 seconds, irradiated area 0.785 cm2, energy density 22,5 J/cm2 and energy delivered 1,1 Joules) after coronary occlusion. The messenger RNAs of Bax and Bcl2 were quantified by PCR - Real Time (Sybr Green). The Western blotting procedure was conducted to evaluate the protein expression of Bax, Bcl2 and caspase 3. Was observed an increase in gene expression and protein of Bcl2 in IM + Laser group when compared to the IM group (p≤0,05), however no significant difference in gene expression of Bax was found. There was a decrease in the expression of cleaved caspase3 the IM group + Laser in relation to the infarcted group. Our results support the hypothesis that low level lasertherapy can stimulate cell survival after a heart attack, possibly by reducing apoptosis of viable area of the heart to maintain cardiac homeostasis after infarctionOs membros da família Bcl-2 são importantes reguladores apoptóticos. As proteínas Bcl-2 podem atuar ativando ou reprimindo a apoptose, podendo contribuir para o surgimento de patologias ou proteger o coração de diversas doenças cardíacas, respectivamente. A caspase-3 é uma protease efetora, cuja clivagem promoverá alterações que resultarão na morte da célula. A modulação de fatores apoptóticos, gerados pela oclusão coronariana e consequente morte celular, após a aplicação do laser de baixa intensidade ainda é pouco estudada. Utilizando modelo experimental, 15 ratas Wistar (200-280g) foram submetidas ao infarto do miocárdio pela oclusão da artéria coronária e randomizadas em três grupos: Controle (ratas controles); IM (ratas infartadas); IM+Laser (ratas infartadas e irradiadas com laser). No grupo IM+Laser, a área infartada foi tratada com laser (λ 660 nm, 15 mW de potência, 60 segundos, área irradiada 0.785 cm2, densidade de energia 22,5 J/cm2, dose 1,1 Joules) após a oclusão coronariana. Os RNAs mensageiros de Bax e Bcl2 foram quantificados por PCR – Real Time (Sybr Green). A técnica de Western blotting foi conduzida para avaliar a expressão protéica de Bax, Bcl 2 e da caspase 3 clivada. Foi observado um aumento na expressão gênica, bem como proteica de Bcl 2 no grupo IM+Laser quando comparado com o grupo IM (p≤0,05), no entanto não se observou diferença significante na expressão gênica de Bax. Houve uma diminuição na expressão da protease caspase 3 no grupo IM+Laser em relação ao grupo infartado. Nossos resultados suportam a hipótese de que a laserterapia de baixa intesidade pode estimular a sobrevivência celular após o infarto, possivelmente por reduzir a apoptose da área viável do coração para manter a homeostase cardíaca após o infarto.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2024-04-08T21:29:10Z No. of bitstreams: 1 Juliana de Almeida Pires.pdf: 1077263 bytes, checksum: 24695f2d040f3447b5e9891e14961374 (MD5)Made available in DSpace on 2024-04-08T21:29:10Z (GMT). No. of bitstreams: 1 Juliana de Almeida Pires.pdf: 1077263 bytes, checksum: 24695f2d040f3447b5e9891e14961374 (MD5) Previous issue date: 2016-08-03application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSaúdeapoptoseCASPASE 3BCL-2BAXinfarto do miocárdiolaser de baixa intensidadeapoptosisCASPASE 3BCL-2BAXmyocardial infarctionlow level laser therapyCIENCIAS DA SAUDEInfluência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratosLow-intensity laser therapy modulation on the expression profile of apoptosis factors BCL2, BAX and cleaved CASPASE 3 after myocardial infarction in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALJuliana de Almeida Pires.pdfJuliana de Almeida Pires.pdfapplication/pdf1077263http://localhost:8080/tede/bitstream/tede/3318/2/Juliana+de+Almeida+Pires.pdf24695f2d040f3447b5e9891e14961374MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/3318/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/33182024-04-08 18:29:10.715oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2024-04-08T21:29:10Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
dc.title.alternative.eng.fl_str_mv Low-intensity laser therapy modulation on the expression profile of apoptosis factors BCL2, BAX and cleaved CASPASE 3 after myocardial infarction in rats
title Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
spellingShingle Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
Pires, Juliana de Almeida
apoptose
CASPASE 3
BCL-2
BAX
infarto do miocárdio
laser de baixa intensidade
apoptosis
CASPASE 3
BCL-2
BAX
myocardial infarction
low level laser therapy
CIENCIAS DA SAUDE
title_short Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
title_full Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
title_fullStr Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
title_full_unstemmed Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
title_sort Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos
author Pires, Juliana de Almeida
author_facet Pires, Juliana de Almeida
author_role author
dc.contributor.advisor1.fl_str_mv Silva Junior, Jose Antonio
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1990427833073161
dc.contributor.referee1.fl_str_mv Silva Junior, Jose Antonio
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1990427833073161
dc.contributor.referee2.fl_str_mv Silva, Carlos Alberto
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/5754147808501113
dc.contributor.referee3.fl_str_mv Serra, Andrey Jorge
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/6499058447489317
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5180416080487653
dc.contributor.author.fl_str_mv Pires, Juliana de Almeida
contributor_str_mv Silva Junior, Jose Antonio
Silva Junior, Jose Antonio
Silva, Carlos Alberto
Serra, Andrey Jorge
dc.subject.por.fl_str_mv apoptose
CASPASE 3
BCL-2
BAX
infarto do miocárdio
laser de baixa intensidade
topic apoptose
CASPASE 3
BCL-2
BAX
infarto do miocárdio
laser de baixa intensidade
apoptosis
CASPASE 3
BCL-2
BAX
myocardial infarction
low level laser therapy
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv apoptosis
CASPASE 3
BCL-2
BAX
myocardial infarction
low level laser therapy
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description Members of the Bcl -2 family are important apoptotic regulators. The Bcl -2 protein can act by activating or repressing apoptosis and may contribute to the emergence of diseases or protect the heart from various heart diseases, respectively. Caspase -3 is an effector protease which cleavage promotes changes that result in cell death. Modulation of apoptotic factors, generated by coronary occlusion and subsequent cell death, after the application of low intensity laser is little studied yet. Using an experimental model, 15 Wistar rats (200-280g) were subjected to myocardial infarction by coronary artery occlusion and randomized into three groups: control (control rats); IM (infarcted rats); IM + Laser (infarcted rats and irradiated with laser). In IM + Laser Group, the infarcted area was treated with laser (λ 660 nm, 15 mW, 60 seconds, irradiated area 0.785 cm2, energy density 22,5 J/cm2 and energy delivered 1,1 Joules) after coronary occlusion. The messenger RNAs of Bax and Bcl2 were quantified by PCR - Real Time (Sybr Green). The Western blotting procedure was conducted to evaluate the protein expression of Bax, Bcl2 and caspase 3. Was observed an increase in gene expression and protein of Bcl2 in IM + Laser group when compared to the IM group (p≤0,05), however no significant difference in gene expression of Bax was found. There was a decrease in the expression of cleaved caspase3 the IM group + Laser in relation to the infarcted group. Our results support the hypothesis that low level lasertherapy can stimulate cell survival after a heart attack, possibly by reducing apoptosis of viable area of the heart to maintain cardiac homeostasis after infarction
publishDate 2016
dc.date.issued.fl_str_mv 2016-08-03
dc.date.accessioned.fl_str_mv 2024-04-08T21:29:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Pires, Juliana de Almeida. Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos. 2016. 73 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/3318
identifier_str_mv Pires, Juliana de Almeida. Influência do laser de baixa intensidade no perfil de expressão dos fatores de apoptose BCL2, BAX e CASPASE 3 clivada após infarto do miocárdio em ratos. 2016. 73 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.
url http://bibliotecatede.uninove.br/handle/tede/3318
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de Mestrado em Medicina
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Saúde
publisher.none.fl_str_mv Universidade Nove de Julho
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da Uninove
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