Avaliação das atividades angiogênica, antiangiogênica, genotóxica e antigenotóxica da spirulina

• Ano de defesa: 2018
• Autor(a) principal: Ciriaco, Robenildo Roney Castro
• Orientador(a): Reis, Paulo Roberto de Melo
• Banca de defesa: Almeida, Luciane Madureira de , Rodrigues, Flávia Melo
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica de Goiás
• Programa de Pós-Graduação: Programa de Pós-Graduação STRICTO SENSU em Ciências Ambientais e Saúde
• Departamento: Escola de Ciências Médicas, Famacêuticas e Biomédicas::Curso de Biomedicina
• País: Brasil
• Palavras-chave em Português: Angiogênese Arthrospira maxima, Citotoxicidade, Membrana Corioalantóide, micronúcleo.
• Palavras-chave em Inglês: Arthrospira maxima, Angiogenesis, Citotoxicidade chorioallantoic membrane, micronucleus.
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: https://tede2.pucgoias.edu.br/handle/tede/4140

Introdution: Spirulina is a cyanobacterium used as a natural product with high nutritional value of proteins and minerals. This algae has several beneficial effects on human health, such as antihypertensive, antioxidant and antihyperlipemic. Currently, Spirulina has been considered a natural drug to prevent and control some diseases. Aim: To evaluate the angiogenic, antiangiogenic, genotoxic and antigenotoxic activity of Spirulina. Methodology: Mice were treated with doses of aqueous solution of Spirulina of 1000 mg/kg, 500 mg/kg or 250 mg/kg by the intraperitoneal route, and the effect genotoxicity and antigenotoxicity was evaluated by micronucleus test after 24 hours. To evaluate the antigenotoxicity the mice was injection using the same doses in association with the doxirubicina.. In addition, antigenic and angiogenic activity was evaluated using embryonated chicken eggs injected with the different Spirulina doses. Descriptive statistics, ANOVA and Tukey were used to analyze the results, by BioEstat 5.0 software. Results: For the genotoxicity tests using spirulina solution, genotoxic activity was demonstrated for all the doses tested. When tested for the antigenotoxic effect of the Spirulina solution, the study showed a significant decrease in micronucleus frequency in 4000 PCE compared to the frequencies of the positive control, considering a p> 0.05, which demonstrates antigenotoxic activity. The results of the angiogenesis assay demonstrated that the three injected doses of Spirulina solution induced a significant decrease in the percentage area of the vascular bundle in the chorioallantoic membrane compared to the control group, indicating an antiangiogenic activity. Conclusion: In conclusion, the Spirulina solution showed genotoxic, antigenotoxic and antiangiogenic activity, however it does not show cytotoxic activity at the concentrations tested by the ratio of normochromatic erythrocytes and polychromatic erythrocytes.