Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas
Ano de defesa: | 2015 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF
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Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/7118 |
Resumo: | The reduction in estrogen concentration, a physiological situation of the female aging, in addition to changes such as increased risk of coronary heart disease, loss of muscle mass and loss of bone mass is also associated with changes in fat composition. Studies from our research laboratory show that ST produces benefits such as increasing the amount of lean body mass, glucose control and reduced lipid content in the liver and muscles. Irisin is known to be a potential therapeutic target in metabolic diseases, including obesity, and its molecular mechanisms are initiated through exercise. Objective: To investigate the effects of ST and HR in plasma levels of Irisin, gene and protein expression of factors involved in the action of Irisin in ovariectomized rats. Methods: 66 rats Holtzman were randomly divided into six groups: 1) Sham-Sed; 2) Sham-ST; 3) Ovx-Sed; 4) Ovx-ST; 5) Ovx-Sed-RH; 6) Ovx-ST-RH. The ST protocol required the animals to climb a 1.1 m-long vertical ladder with weights atteched to their tails. Each session consisted of 4 to 8 climbs with 2 minutes interval between climbing, performed once every three days for 12 weeks. Analyses of PGC1-α mRNA expression, and FNDC5 and UCP1 were performed by RT-PCR ΔΔCt method, protein expression analysis of FNDC5 and UCP1 were performed by the Western blotting technique, Irisin and 17β-estradiol levels were determined by ELISA. Results: Our results show that the Sham, Ovx and HR groups who underwent 12 weeks of ST had a higher expression of the Irisin action mechanism, with higher mRNA expression of PGC-1α in the gastrocnemius muscle, increased protein expression FNDC5, higher Irisin concentration in the plasma and increased protein expression of UCP1 in mesenteric fat. Conclusion: Ovariectomized rats had lower circulating concentration Irisina, which promotes browning of white adipose tissue, however, the ST for 12 weeks was an effective intervention strategy to contain higher circulating concentration of Irisin in intact and ovariectomized rats. And the TF associated with RH was an effective intervention strategy to contain higher circulating concentration of Irisin in ovariectomized. |
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Magosso, Natalia Santanielo SilvaPerez, Sergio Eduardo de Andradehttp://lattes.cnpq.br/9408953831211309http://lattes.cnpq.br/28494232581498747e79a989-fbf8-4f09-af7a-84b2ec454c0d2016-09-13T18:53:25Z2016-09-13T18:53:25Z2015-09-04MAGOSSO, Natalia Santanielo Silva. Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas. 2015. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2015. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7118.https://repositorio.ufscar.br/handle/ufscar/7118The reduction in estrogen concentration, a physiological situation of the female aging, in addition to changes such as increased risk of coronary heart disease, loss of muscle mass and loss of bone mass is also associated with changes in fat composition. Studies from our research laboratory show that ST produces benefits such as increasing the amount of lean body mass, glucose control and reduced lipid content in the liver and muscles. Irisin is known to be a potential therapeutic target in metabolic diseases, including obesity, and its molecular mechanisms are initiated through exercise. Objective: To investigate the effects of ST and HR in plasma levels of Irisin, gene and protein expression of factors involved in the action of Irisin in ovariectomized rats. Methods: 66 rats Holtzman were randomly divided into six groups: 1) Sham-Sed; 2) Sham-ST; 3) Ovx-Sed; 4) Ovx-ST; 5) Ovx-Sed-RH; 6) Ovx-ST-RH. The ST protocol required the animals to climb a 1.1 m-long vertical ladder with weights atteched to their tails. Each session consisted of 4 to 8 climbs with 2 minutes interval between climbing, performed once every three days for 12 weeks. Analyses of PGC1-α mRNA expression, and FNDC5 and UCP1 were performed by RT-PCR ΔΔCt method, protein expression analysis of FNDC5 and UCP1 were performed by the Western blotting technique, Irisin and 17β-estradiol levels were determined by ELISA. Results: Our results show that the Sham, Ovx and HR groups who underwent 12 weeks of ST had a higher expression of the Irisin action mechanism, with higher mRNA expression of PGC-1α in the gastrocnemius muscle, increased protein expression FNDC5, higher Irisin concentration in the plasma and increased protein expression of UCP1 in mesenteric fat. Conclusion: Ovariectomized rats had lower circulating concentration Irisina, which promotes browning of white adipose tissue, however, the ST for 12 weeks was an effective intervention strategy to contain higher circulating concentration of Irisin in intact and ovariectomized rats. And the TF associated with RH was an effective intervention strategy to contain higher circulating concentration of Irisin in ovariectomized.A redução da concentração de estrógeno, situação fisiológica do envelhecimento feminino, além de alterações como aumento do risco de doença coronariana, perda de massa muscular e perda de massa óssea, também está associada a alterações da composição de gordura. Estudos do nosso laboratório de pesquisa mostram que o TF produz benefícios como aumento da quantidade de massa magra, controle glicêmico e redução do conteúdo de lipídeos no fígado e nos músculos. A Irisina é conhecida por ser um potencial alvo terapêutico em doenças metabólicas, incluindo a obesidade, e os seus mecanismos moleculares iniciam através do exercício físico. Objetivo: Investigar os efeitos do TF e da RH nos níveis plasmáticos de Irisina, expressão gênica e proteica de fatores envolvidos na ação de Irisina em ratas ovariectomizadas. Metodologia: 66 ratas Holtzman foram randomicamente distribuídas em seis grupos experimentais: 1) Sham-Sed; 2) Sham-TF; 3) Ovx-Sed; 4) Ovx-TF; 5) Ovx-Sed-RH; 6) Ovx-TF-RH. O protocolo TF exigiu dos animais a escalada vertical de 1,1 m com pesos atados às suas caudas. Cada sessão consistiu de 4 a 8 escaladas, com intervalo de 2 minutos entre as escaladas, realizadas uma vez a cada três dias durante 12 semanas. As análises de expressão de RNAm de PGC1-α, FNDC5 e UCP1 foram realizadas por PCR-RT pelo método ΔΔCt, as análises de expressão proteica de FNDC5 e UCP1 foram realizadas pela técnica de Western Blotting e as análises de concentração de Irisina e 17β-estradiol foram determinadas por ELISA. Resultados: Os nossos resultados indicam que os grupos Sham, Ovx e RH que realizaram 12 semanas de TF apresentaram respostas positivas no mecanismo de ação de Irisina, apresentando maior expressão de RNAm de PGC-1α no musculo gastrocnêmio, maior expressão proteica de FNDC5, maior concentração de Irisina no plasma e maior expressão proteica de UCP1 na gordura mesentérica. Conclusão: As ratas ovariectomizadas apresentaram menor concentração circulante Irisina, que favorecem o escurecimento do tecido adiposo branco, porém, o TF por 12 semanas foi uma estratégia de intervenção eficaz para apresentar maior concentração circulante de Irisina em ratas intactas e ovariectomizadas. E o TF associado à RH foi uma estratégia de intervenção eficaz para apresentar maior concentração circulante de Irisina em ovariectomizadas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)porUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarOvariectomiaReposição hormonalTreinamento de forçaIrisinaOvariectomyHormone replacement therapyStrength trainingIrisinCIENCIAS BIOLOGICAS::FISIOLOGIAEfeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline60060094c0c56d-5b6c-4622-8f35-ef5d57c5c02cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissNSSM.pdfDissNSSM.pdfapplication/pdf1469317https://repositorio.ufscar.br/bitstream/ufscar/7118/1/DissNSSM.pdf498631be20a405584e3056f51e54c15fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/7118/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissNSSM.pdf.txtDissNSSM.pdf.txtExtracted texttext/plain91999https://repositorio.ufscar.br/bitstream/ufscar/7118/3/DissNSSM.pdf.txtc91a8394bab463d0935eb45489d40524MD53THUMBNAILDissNSSM.pdf.jpgDissNSSM.pdf.jpgIM Thumbnailimage/jpeg7532https://repositorio.ufscar.br/bitstream/ufscar/7118/4/DissNSSM.pdf.jpg9a968fac7e73144559a1d09cdb7801afMD54ufscar/71182023-09-18 18:30:43.25oai:repositorio.ufscar.br:ufscar/7118TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlCkZlZGVyYWwgZGUgU8OjbyBDYXJsb3MgbyBkaXJlaXRvIG7Do28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsICB0cmFkdXppciAoY29uZm9ybWUgZGVmaW5pZG8gYWJhaXhvKSwgZS9vdQpkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlCmVtIHF1YWxxdWVyIG1laW8sIGluY2x1aW5kbyBvcyBmb3JtYXRvcyDDoXVkaW8gb3UgdsOtZGVvLgoKVm9jw6ogY29uY29yZGEgcXVlIGEgVUZTQ2FyIHBvZGUsIHNlbSBhbHRlcmFyIG8gY29udGXDumRvLCB0cmFuc3BvciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28KcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVGU0NhciBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgYSBzdWEgdGVzZSBvdQpkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcwpuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldQpjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6oKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVGU0NhcgpvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUKaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBvcmEgZGVwb3NpdGFkYS4KCkNBU08gQSBURVNFIE9VIERJU1NFUlRBw4fDg08gT1JBIERFUE9TSVRBREEgVEVOSEEgU0lETyBSRVNVTFRBRE8gREUgVU0gUEFUUk9Dw41OSU8gT1UKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRlNDYXIsClZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PClRBTULDiU0gQVMgREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRlNDYXIgc2UgY29tcHJvbWV0ZSBhIGlkZW50aWZpY2FyIGNsYXJhbWVudGUgbyBzZXUgbm9tZSAocykgb3UgbyhzKSBub21lKHMpIGRvKHMpCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzCmNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuCg==Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:43Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
title |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
spellingShingle |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas Magosso, Natalia Santanielo Silva Ovariectomia Reposição hormonal Treinamento de força Irisina Ovariectomy Hormone replacement therapy Strength training Irisin CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
title_full |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
title_fullStr |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
title_full_unstemmed |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
title_sort |
Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas |
author |
Magosso, Natalia Santanielo Silva |
author_facet |
Magosso, Natalia Santanielo Silva |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/2849423258149874 |
dc.contributor.author.fl_str_mv |
Magosso, Natalia Santanielo Silva |
dc.contributor.advisor1.fl_str_mv |
Perez, Sergio Eduardo de Andrade |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9408953831211309 |
dc.contributor.authorID.fl_str_mv |
7e79a989-fbf8-4f09-af7a-84b2ec454c0d |
contributor_str_mv |
Perez, Sergio Eduardo de Andrade |
dc.subject.por.fl_str_mv |
Ovariectomia Reposição hormonal Treinamento de força Irisina |
topic |
Ovariectomia Reposição hormonal Treinamento de força Irisina Ovariectomy Hormone replacement therapy Strength training Irisin CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
Ovariectomy Hormone replacement therapy Strength training Irisin |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
The reduction in estrogen concentration, a physiological situation of the female aging, in addition to changes such as increased risk of coronary heart disease, loss of muscle mass and loss of bone mass is also associated with changes in fat composition. Studies from our research laboratory show that ST produces benefits such as increasing the amount of lean body mass, glucose control and reduced lipid content in the liver and muscles. Irisin is known to be a potential therapeutic target in metabolic diseases, including obesity, and its molecular mechanisms are initiated through exercise. Objective: To investigate the effects of ST and HR in plasma levels of Irisin, gene and protein expression of factors involved in the action of Irisin in ovariectomized rats. Methods: 66 rats Holtzman were randomly divided into six groups: 1) Sham-Sed; 2) Sham-ST; 3) Ovx-Sed; 4) Ovx-ST; 5) Ovx-Sed-RH; 6) Ovx-ST-RH. The ST protocol required the animals to climb a 1.1 m-long vertical ladder with weights atteched to their tails. Each session consisted of 4 to 8 climbs with 2 minutes interval between climbing, performed once every three days for 12 weeks. Analyses of PGC1-α mRNA expression, and FNDC5 and UCP1 were performed by RT-PCR ΔΔCt method, protein expression analysis of FNDC5 and UCP1 were performed by the Western blotting technique, Irisin and 17β-estradiol levels were determined by ELISA. Results: Our results show that the Sham, Ovx and HR groups who underwent 12 weeks of ST had a higher expression of the Irisin action mechanism, with higher mRNA expression of PGC-1α in the gastrocnemius muscle, increased protein expression FNDC5, higher Irisin concentration in the plasma and increased protein expression of UCP1 in mesenteric fat. Conclusion: Ovariectomized rats had lower circulating concentration Irisina, which promotes browning of white adipose tissue, however, the ST for 12 weeks was an effective intervention strategy to contain higher circulating concentration of Irisin in intact and ovariectomized rats. And the TF associated with RH was an effective intervention strategy to contain higher circulating concentration of Irisin in ovariectomized. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-09-04 |
dc.date.accessioned.fl_str_mv |
2016-09-13T18:53:25Z |
dc.date.available.fl_str_mv |
2016-09-13T18:53:25Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MAGOSSO, Natalia Santanielo Silva. Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas. 2015. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2015. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7118. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/7118 |
identifier_str_mv |
MAGOSSO, Natalia Santanielo Silva. Efeitos do treinamento de força e da reposição hormonal no mecanismo de ação de irisina de ratas ovariectomizadas. 2015. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2015. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7118. |
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https://repositorio.ufscar.br/handle/ufscar/7118 |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Universidade Federal de São Carlos Câmpus São Carlos |
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