Ácidos húmicos extraídos do lodo de esgoto sanitário e seus efeitos em plantas

• Ano de defesa: 2014
• Autor(a) principal: Rocha, Lívia Dorsch
• Orientador(a): Matsumoto, Silvia Tamie
• Banca de defesa: Cuzzuol, Geraldo Rogério Faustini, Mazzeo, Dânia Elisa Christofoletti
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; Mestrado em Biologia Vegetal
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Vegetal
• País: BR
• Palavras-chave em Português: Allium cepa; Oryza sativa
• Palavras-chave em Inglês: Humic acid; Enzymes of oxidative stress; Plant growth
• Área do conhecimento CNPq: Agronomia
• Link de acesso: http://repositorio.ufes.br/handle/10/1248

The final disposal of sewage sludge has been presented as an environmental and public health problem. The sewage sludge is rich in organic matter but it also presents pollutants and pathogens. Thus, this work was extracted humic acid (HA) which is a compound resulting from the fractionation of humic substances that comprises a carbon group generated by the decomposition of organic waste that suffer resynthesis forming humus. Humic acid promotes many benefits in plants, such as growth cell elongation, stress tolerance, increased plasma membrane permeability. Based on these features, the present study aimed to evaluate the cytogenetic effects (Allium cepa test), physiological and biochemical in Oryza sativa culture treated with differents concentrations of humic acids derived from sewage sludge. Elemental characterization of HA was performed to define the doses (0,5; 1, 2 e 4 mM C L-1). After 20 days of treatment, samples of the material were taken and subsequently analyzed. The chemical characterization of HA indicated it as a good conditioner for cultures showing high rates of Carbon, Hydrogen and Nitrogen. No effect of toxicity, cytotoxicity, genotoxicity and mutagenicity of HA was observed. The results have shown significant increase of plant photosynthetic pigments on higher concentrations (2 mM C L-1 and 4 mM C L-1). In general the higher concentrations have presented more growth. Futhermore, there was an increase of ATPase expression in all treatments. And enzymes of oxidative stress increased relatively in differents concentrations. By the integration of these analyzes it was possible conclude that the humic acid from sewage sludge can be used as organic fertilizer as observed by the plant benefits showed in this work. Moreover, HA offers a good alternative for the disposal of sewage sludge residue, recycling the organic matter.