Otimização e desenvolvimento de sistemas de detecção de microcistinas em amostras de águas

• Ano de defesa: 2013
• Autor(a) principal: Antunes, Paulo Wagnner Pereira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Doutorado em Engenharia Ambiental; Centro Tecnológico; UFES; Programa de Pós-Graduação em Engenharia Ambiental
• Palavras-chave em Português: Microcystin detection methods; water quality; monitoring program; Microcistina; Método qualitativo; Validação e monitoramento; CLAE; Água - Qualidade; Cromatografia a líquido de alta eficiência; Água - Análise; Engenharia Sanitária; 628
• Link de acesso: http://repositorio.ufes.br/handle/10/3917

The monitoring program performed with surface water samples of the metropolitan region of Vitoria, ES, showed the presence of one variant of microcystin in 57% of samples. With 20% of these samples the concentration of microcystin was greater than 1.0 µg / L, the maximum concentration allowed by Brazilian legislation for public water supply. Few laboratories in the state of Espírito Santo have appropriate infrastructure for the analysis of microcystins and furthermore, the current quantitative methods are costly and time consuming. With the main objective to develop a system for microcystin detection, in this study a quantitative method was validated by high performance liquid chromatography (HPLC) and established a qualitative system (P / A) for analysis of microcystins. The validated method free of acetonitrile showed linearity and selectivity to separate and quantify different microcystin variants (-RR,-YR,-LR and-LA). Recovery levels between 98.2 and 106.1% have demonstrated the precision and detection limits (from 0.17 to 0.25 g / L) and quantification (between 0.55 and 0.82 g / L) met the national and international standard limits for drinking water. The qualitative system (P / A) was proven to be easy to use, low cost and high sensitivity, allowing direct visual determination of the presence of microcystin in concentrations above 0.80 mg / L, without the need for any processing method , cleaning or concentration of the sample. Compared with traditional microcystin detection methods such as ELISA and HPLC it was shown the system reliability rates of 82.4% and 88.2%.