Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos
Ano de defesa: | 2011 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Programa de Pós-graduação em Patologia
Patologia |
Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
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País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Link de acesso: | https://app.uff.br/riuff/handle/1/19426 |
Resumo: | Mast cells are found in the connective tissue and can participate in acute and chronic inflammatory responses and induce early and also late-phase reactions. Mast cells are distributed in odontogenic cysts and tumors and it has been suggested that mast cell degranulation products can be related to matrix degradation in the cystic or tumor and to the production of cytokines, which appear to have important functions in bone resorption and cystic growth. The aim of this study was to evaluate the frequency and distribution of mast cells in periapical and dentigerous cysts, and keratocystic odontogenic tumors. Paraffin embedded specimens from 20 periapical cysts, 20 dentigerous cysts (14 with and six without inflammation) and 20 keratocystic odontogenic tumors (16 with and four without inflammation) were selected from the files of the Department of Pathology of the Antonio Pedro Universitary Hospital/Fluminense Federal University. Hematoxylin and eosin stained slides were reviewed through optical microscopy in all cases for diagnosis confirmation by light microscopy. Demographic, clinical and radiographic data from all cases were retrieved from the laboratory records. For observation of mast cells, all 60 cases were evaluated through toluidine blue staining and immunohistochemical reaction with antibody directed against mast cell tryptase. Mast cells were quantified using the Image Pro-Plus 4.5 program (Media Cybernetics, USA) by two techniques in 10 high power fields (40x) and was obtained the mean number of mast cells in three locations: epithelial, superficial fibrous wall and deep fibrous wall. The mean number of mast cells in each region was compared in the three groups of lesions and also was compared the mean number between the different locations to each one of the lesions studied, giving their quantification and distribution profile and descriptive comparison. The distribution of mast cells evaluated through immunohistochemistry was higher in the group of inflamed dentigerous cysts (2,9 cells/high power field), followed by periapical cysts (1,5 cells/high power field), inflamed keratocystic odontogenic tumor (1,3 cells/high power field) and non inflamed dentigerous cysts (0) and non inflammed keratocystic odontogenic tumor (0). In contrast, the distribution of mast cells observed through immunohistochemistry revealed that periapical cysts showed the higher mean (5,3 cells/high power field), followed by inflamed dentigerous cysts (4,7 cells/high power field), inflamed keratocystic odontogenic tumor (3,7 cells/high power field), non inflamed keratocystic odontogenic tumor (2 cells/high power field) and non inflamed dentigerous cyst (0,9 cells/high power field). The deep portion of the fibrous wall presented the higher mean of the mast cells in the three groups of lesions studied, through both techniques, in comparison to the epithelial and superficial regions, except for the non inflamed keratocystic odontogenic tumor analyzed through immunohistochemistry, which showed a higher number of mast cells in the superficial region of the fibrous wall. The increased number of mast cells in lesions with inflammation and at sities of inflammation indicates the participation of these cells in the inflammatory response. The predominance of mast cells, a lot of them degranulated, in the deep region of the fibrous cystic wall may suggest a higher cellular activity possibly related to bone resorption and growth of these lesions. More studies investigating the mediators of inflammation and bone resorption are needed to confirm the role of mast cells in the pathogenesis and behavior of the cystic lesions |
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Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticosMastócitosCisto periapicalCisto dentígeroTumor odontogênico ceratocísticoMEDICINAPATOLOGIA HUMANACistos odontogênicosCisto radicularMast cellsPeriapical cystDentigerous cystKeratocystic odontogenic tumorCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICAMast cells are found in the connective tissue and can participate in acute and chronic inflammatory responses and induce early and also late-phase reactions. Mast cells are distributed in odontogenic cysts and tumors and it has been suggested that mast cell degranulation products can be related to matrix degradation in the cystic or tumor and to the production of cytokines, which appear to have important functions in bone resorption and cystic growth. The aim of this study was to evaluate the frequency and distribution of mast cells in periapical and dentigerous cysts, and keratocystic odontogenic tumors. Paraffin embedded specimens from 20 periapical cysts, 20 dentigerous cysts (14 with and six without inflammation) and 20 keratocystic odontogenic tumors (16 with and four without inflammation) were selected from the files of the Department of Pathology of the Antonio Pedro Universitary Hospital/Fluminense Federal University. Hematoxylin and eosin stained slides were reviewed through optical microscopy in all cases for diagnosis confirmation by light microscopy. Demographic, clinical and radiographic data from all cases were retrieved from the laboratory records. For observation of mast cells, all 60 cases were evaluated through toluidine blue staining and immunohistochemical reaction with antibody directed against mast cell tryptase. Mast cells were quantified using the Image Pro-Plus 4.5 program (Media Cybernetics, USA) by two techniques in 10 high power fields (40x) and was obtained the mean number of mast cells in three locations: epithelial, superficial fibrous wall and deep fibrous wall. The mean number of mast cells in each region was compared in the three groups of lesions and also was compared the mean number between the different locations to each one of the lesions studied, giving their quantification and distribution profile and descriptive comparison. The distribution of mast cells evaluated through immunohistochemistry was higher in the group of inflamed dentigerous cysts (2,9 cells/high power field), followed by periapical cysts (1,5 cells/high power field), inflamed keratocystic odontogenic tumor (1,3 cells/high power field) and non inflamed dentigerous cysts (0) and non inflammed keratocystic odontogenic tumor (0). In contrast, the distribution of mast cells observed through immunohistochemistry revealed that periapical cysts showed the higher mean (5,3 cells/high power field), followed by inflamed dentigerous cysts (4,7 cells/high power field), inflamed keratocystic odontogenic tumor (3,7 cells/high power field), non inflamed keratocystic odontogenic tumor (2 cells/high power field) and non inflamed dentigerous cyst (0,9 cells/high power field). The deep portion of the fibrous wall presented the higher mean of the mast cells in the three groups of lesions studied, through both techniques, in comparison to the epithelial and superficial regions, except for the non inflamed keratocystic odontogenic tumor analyzed through immunohistochemistry, which showed a higher number of mast cells in the superficial region of the fibrous wall. The increased number of mast cells in lesions with inflammation and at sities of inflammation indicates the participation of these cells in the inflammatory response. The predominance of mast cells, a lot of them degranulated, in the deep region of the fibrous cystic wall may suggest a higher cellular activity possibly related to bone resorption and growth of these lesions. More studies investigating the mediators of inflammation and bone resorption are needed to confirm the role of mast cells in the pathogenesis and behavior of the cystic lesionsMastócitos são células localizadas no tecido conjuntivo que participam de diversas respostas inflamatórias agudas e crônicas, induzindo respostas de reação imediata e tardia. Têm sido encontrados em diversos cistos e tumores odontogênicos e sugere-se que seus produtos de desgranulação, incluindo enzimas e citocinas, possam estar relacionados à degradação da matriz conjuntiva na parede cística ou tumoral e ser importantes no processo de reabsorção óssea, facilitando o crescimento destas lesões. Este estudo teve por objetivo avaliar a frequência e a distribuição dos mastócitos em lesões odontogênicas de morfologia cística e, para isso, foram utilizados espécimes incluídos em parafina de 20 cistos periapicais, 20 cistos dentígeros (seis sem inflamação e 14 com inflamação) e 20 tumores odontogênicos ceratocísticos (quatro sem inflamação e 16 com inflamação) oriundos do arquivo do Serviço de Anatomia Patológica do Hospital Universitário Antonio Pedro/Universidade Federal Fluminense. Todos os casos foram revisados microscopicamente na coloração de hematoxilina e eosina para confirmação do diagnóstico através de microscopia óptica. Os dados demográficos e clínico-radiográficos de todos os casos foram obtidos através de consulta às requisições laboratoriais. Para a avaliação dos mastócitos os 60 casos foram avaliados pela coloração de azul de toluidina e pela reação de imuno-histoquímica da expressão do anticorpo anti-triptase para mastócitos. Os mastócitos foram quantificados com auxílio do programa Image Pro-Plus 4.5 (Media Cybernetics, EUA) pelas duas técnicas em 10 campos de grande aumento (40x) e foi obtido o índice médio do número de mastócitos em três localizações: epitelial, parede fibrosa superficial e parede fibrosa profunda. A média do número de mastócitos de cada região foi comparada entre os grupos de lesões estudadas, assim como foram comparadas as médias entre as diferentes localizações para cada uma das lesões, permitindo obter sua quantificação e o perfil de distribuição e comparação descritiva. A média de distribuição de mastócitos por campo por meio da técnica histoquímica foi superior no grupo dos cistos dentígeros inflamados (2,9 células) do que no cisto periapical (1,5 células), tumor odontogênico ceratocístico inflamado (1,3 células) e cistos dentígeros e tumores odontogênicos ceratocisticos não inflamados (0). Entretanto, pela técnica imuno-histoquímica, o grupo de lesões que apresentou a maior média do número de células foi o do cisto periapical (5,3 células) seguido do cisto dentígero inflamado (4,7 células), tumor odontogênico ceratocístico inflamado (3,7 células) e não inflamado (2 células) e cisto dentígero não inflamado (0,9 células). A região profunda das lesões em ambas as técnicas, apresentou a maior média de mastócitos, à exceção do tumor odontogênico ceratocístico não inflamado, que na técnica imuno-histoquímica apresentou maior número de mastócitos na região superficial. A maior quantidade de mastócitos nas lesões com inflamação indica a participação dessas células na resposta inflamatória nestas entidades. O predomínio de mastócitos, muito deles desgranulados, na parede cística profunda das lesões, sugere maior atividade celular possivelmente relacionada à reabsorção óssea e crescimento das lesões. Mais estudos investigando os mediadores da inflamação e da reabsorção óssea são necessários para confirmar o papel dos mastócitos na patogênese e no comportamento das lesões císticas odontogênicasPrograma de Pós-graduação em PatologiaPatologiaLourenço, Simone de Queiroz ChavesCPF:01456525727http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795619T7Pires, Fábio RamôaCPF:01384934723http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768254A9&dataRevisao=nullCabral, Márcia GrilloCPF:88322017715http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704341Y5&dataRevisao=nullRochael, Mayra CarrijoCPF:43455321122http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797553J7Siqueira Junior, José FreitasCPF:78589595822http://lattes.cnpq.br/3006383510997981Netto, Juliana de Noronha Santos2021-03-10T20:47:24Z2011-12-072021-03-10T20:47:24Z2011-11-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/19426porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:47:24Zoai:app.uff.br:1/19426Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T11:11:05.503859Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
title |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
spellingShingle |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos Netto, Juliana de Noronha Santos Mastócitos Cisto periapical Cisto dentígero Tumor odontogênico ceratocístico MEDICINA PATOLOGIA HUMANA Cistos odontogênicos Cisto radicular Mast cells Periapical cyst Dentigerous cyst Keratocystic odontogenic tumor CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
title_short |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
title_full |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
title_fullStr |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
title_full_unstemmed |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
title_sort |
Avaliação de mastócitos em cistos periapicais, cistos dentígeros e tumores odontogênicos ceratocísticos |
author |
Netto, Juliana de Noronha Santos |
author_facet |
Netto, Juliana de Noronha Santos |
author_role |
author |
dc.contributor.none.fl_str_mv |
Lourenço, Simone de Queiroz Chaves CPF:01456525727 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795619T7 Pires, Fábio Ramôa CPF:01384934723 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768254A9&dataRevisao=null Cabral, Márcia Grillo CPF:88322017715 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704341Y5&dataRevisao=null Rochael, Mayra Carrijo CPF:43455321122 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797553J7 Siqueira Junior, José Freitas CPF:78589595822 http://lattes.cnpq.br/3006383510997981 |
dc.contributor.author.fl_str_mv |
Netto, Juliana de Noronha Santos |
dc.subject.por.fl_str_mv |
Mastócitos Cisto periapical Cisto dentígero Tumor odontogênico ceratocístico MEDICINA PATOLOGIA HUMANA Cistos odontogênicos Cisto radicular Mast cells Periapical cyst Dentigerous cyst Keratocystic odontogenic tumor CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
topic |
Mastócitos Cisto periapical Cisto dentígero Tumor odontogênico ceratocístico MEDICINA PATOLOGIA HUMANA Cistos odontogênicos Cisto radicular Mast cells Periapical cyst Dentigerous cyst Keratocystic odontogenic tumor CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
description |
Mast cells are found in the connective tissue and can participate in acute and chronic inflammatory responses and induce early and also late-phase reactions. Mast cells are distributed in odontogenic cysts and tumors and it has been suggested that mast cell degranulation products can be related to matrix degradation in the cystic or tumor and to the production of cytokines, which appear to have important functions in bone resorption and cystic growth. The aim of this study was to evaluate the frequency and distribution of mast cells in periapical and dentigerous cysts, and keratocystic odontogenic tumors. Paraffin embedded specimens from 20 periapical cysts, 20 dentigerous cysts (14 with and six without inflammation) and 20 keratocystic odontogenic tumors (16 with and four without inflammation) were selected from the files of the Department of Pathology of the Antonio Pedro Universitary Hospital/Fluminense Federal University. Hematoxylin and eosin stained slides were reviewed through optical microscopy in all cases for diagnosis confirmation by light microscopy. Demographic, clinical and radiographic data from all cases were retrieved from the laboratory records. For observation of mast cells, all 60 cases were evaluated through toluidine blue staining and immunohistochemical reaction with antibody directed against mast cell tryptase. Mast cells were quantified using the Image Pro-Plus 4.5 program (Media Cybernetics, USA) by two techniques in 10 high power fields (40x) and was obtained the mean number of mast cells in three locations: epithelial, superficial fibrous wall and deep fibrous wall. The mean number of mast cells in each region was compared in the three groups of lesions and also was compared the mean number between the different locations to each one of the lesions studied, giving their quantification and distribution profile and descriptive comparison. The distribution of mast cells evaluated through immunohistochemistry was higher in the group of inflamed dentigerous cysts (2,9 cells/high power field), followed by periapical cysts (1,5 cells/high power field), inflamed keratocystic odontogenic tumor (1,3 cells/high power field) and non inflamed dentigerous cysts (0) and non inflammed keratocystic odontogenic tumor (0). In contrast, the distribution of mast cells observed through immunohistochemistry revealed that periapical cysts showed the higher mean (5,3 cells/high power field), followed by inflamed dentigerous cysts (4,7 cells/high power field), inflamed keratocystic odontogenic tumor (3,7 cells/high power field), non inflamed keratocystic odontogenic tumor (2 cells/high power field) and non inflamed dentigerous cyst (0,9 cells/high power field). The deep portion of the fibrous wall presented the higher mean of the mast cells in the three groups of lesions studied, through both techniques, in comparison to the epithelial and superficial regions, except for the non inflamed keratocystic odontogenic tumor analyzed through immunohistochemistry, which showed a higher number of mast cells in the superficial region of the fibrous wall. The increased number of mast cells in lesions with inflammation and at sities of inflammation indicates the participation of these cells in the inflammatory response. The predominance of mast cells, a lot of them degranulated, in the deep region of the fibrous cystic wall may suggest a higher cellular activity possibly related to bone resorption and growth of these lesions. More studies investigating the mediators of inflammation and bone resorption are needed to confirm the role of mast cells in the pathogenesis and behavior of the cystic lesions |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-07 2011-11-28 2021-03-10T20:47:24Z 2021-03-10T20:47:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/19426 |
url |
https://app.uff.br/riuff/handle/1/19426 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
instacron_str |
UFF |
institution |
UFF |
reponame_str |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
collection |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
repository.mail.fl_str_mv |
riuff@id.uff.br |
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1817064866980036608 |