Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC.
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Mestrado em Ciências Farmacêuticas
|
Departamento: |
Ciências da Saúde - Farmácia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tde/2093 |
Resumo: | The Brazilian Savanna is recognized as the richest in the world, sheltering more than 6.500 plant species already cataloged, and medicinal uses are attributed to over 220. Species of Myrtaceae family are used in folk medicine in gastrointestinal disorders, infectious diseases and hemorrhagic states, many are known by the presence of essential oils. The objective of this work was the pharmacognostic study of leaves and bark of species Myrcia tomentosa (Aubl.) DC. in order to establish quality parameters and to determine the chemical composition of essential oil (leaves, bark and flowers), as well as to assess the occurrence of seasonal variation between the chemical constituents of essential oil from leaves and to evaluate the antimicrobial activity of leaf essential oil, ethanolic crude extract and fractions from leaf and bark extracts. For this purpose, part of the botanical materials was used in microscopic analysis and in phytochemical analysis. The contents of total phenols, tannins and of total flavonoids were evaluated. The gas chromatography coupled to mass spectrometry (GC / MS) was used to characterize the chemistry of essential oils and the broth microdilution test was used for both evaluation of antimicrobial activity and determination of Minimum Inhibitory Concentration (MIC). In morpho-anatomical study of leaves, it was found that they are simple, leathery and predominantly opposing crossed. Microscopically are hypostomatic with numerous unicellular trichomes, have secretory cavities and idioblasts with prismatic crystals. The bark has flat, curved and sometimes channeled shape. At microscopic level, the bark shows prismatic crystals, sclereids and periderm is formed in the inner layers. In the phytochemical screening, it was found essential oils, tannins and terpenes in leaves and bark, only flavonoids in leaves and saponin in bark. In the essential oils analysis, it was found that (2E, 6E)-Methyl farnesoate is the major component in the flowers and bark essential oils, while the leaves essential oil showed seasonal variation in their chemical composition, with Bicyclogermacrene and (2E, 6E)-Methyl farnesoate as major components The antimicrobial evaluation of leaf essential oil showed activity for Micrococcus roseus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and Staphylococcus aureus (MIC = 125 - 250 μg/mL) and the fungi Cryptococcus neoformans and Cryptococcus gattii (MIC = 62,5 500 μg/mL); the fractions were moderate activity for most Gram-positive bacteria, and the hexane and dichlorometane fractions of the leaves, and ethyl acetate and aqueous bark were most active (MIC = 250 500 μg/mL). With respect to antifungal activity, the fractions tested showed good or moderate activity for Candida sp (MIC = 15,62 125 μg/mL) and hexanic and dichloromethanic fractions of leaves showed good activity for Cryptococcus sp (MIC = 15,62 62,50 μg/mL). The results contribute to the identification of the species M. tomentosa and demontrate its potential for the treatment of fungal diseases. |
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PAULA, José Realino dehttp://lattes.cnpq.br/3191837532986128http://lattes.cnpq.br/7688986744408368SÁ, Fabyola Amaral da Silva2014-07-29T16:11:46Z2011-01-262010-11-05SÁ, Fabyola Amaral da Silva. Morphology, anatomy, chemical composition of essential oil and antimicrobial activity of the leaves and stem bark of Myrcia tomentosa (Aubl.) DC.. 2010. 27 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2010.http://repositorio.bc.ufg.br/tede/handle/tde/2093ark:/38995/00130000091g8The Brazilian Savanna is recognized as the richest in the world, sheltering more than 6.500 plant species already cataloged, and medicinal uses are attributed to over 220. Species of Myrtaceae family are used in folk medicine in gastrointestinal disorders, infectious diseases and hemorrhagic states, many are known by the presence of essential oils. The objective of this work was the pharmacognostic study of leaves and bark of species Myrcia tomentosa (Aubl.) DC. in order to establish quality parameters and to determine the chemical composition of essential oil (leaves, bark and flowers), as well as to assess the occurrence of seasonal variation between the chemical constituents of essential oil from leaves and to evaluate the antimicrobial activity of leaf essential oil, ethanolic crude extract and fractions from leaf and bark extracts. For this purpose, part of the botanical materials was used in microscopic analysis and in phytochemical analysis. The contents of total phenols, tannins and of total flavonoids were evaluated. The gas chromatography coupled to mass spectrometry (GC / MS) was used to characterize the chemistry of essential oils and the broth microdilution test was used for both evaluation of antimicrobial activity and determination of Minimum Inhibitory Concentration (MIC). In morpho-anatomical study of leaves, it was found that they are simple, leathery and predominantly opposing crossed. Microscopically are hypostomatic with numerous unicellular trichomes, have secretory cavities and idioblasts with prismatic crystals. The bark has flat, curved and sometimes channeled shape. At microscopic level, the bark shows prismatic crystals, sclereids and periderm is formed in the inner layers. In the phytochemical screening, it was found essential oils, tannins and terpenes in leaves and bark, only flavonoids in leaves and saponin in bark. In the essential oils analysis, it was found that (2E, 6E)-Methyl farnesoate is the major component in the flowers and bark essential oils, while the leaves essential oil showed seasonal variation in their chemical composition, with Bicyclogermacrene and (2E, 6E)-Methyl farnesoate as major components The antimicrobial evaluation of leaf essential oil showed activity for Micrococcus roseus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and Staphylococcus aureus (MIC = 125 - 250 μg/mL) and the fungi Cryptococcus neoformans and Cryptococcus gattii (MIC = 62,5 500 μg/mL); the fractions were moderate activity for most Gram-positive bacteria, and the hexane and dichlorometane fractions of the leaves, and ethyl acetate and aqueous bark were most active (MIC = 250 500 μg/mL). With respect to antifungal activity, the fractions tested showed good or moderate activity for Candida sp (MIC = 15,62 125 μg/mL) and hexanic and dichloromethanic fractions of leaves showed good activity for Cryptococcus sp (MIC = 15,62 62,50 μg/mL). The results contribute to the identification of the species M. tomentosa and demontrate its potential for the treatment of fungal diseases.O Cerrado brasileiro é reconhecido como a savana mais rica do mundo, abrigando mais de 6.500 espécies de plantas já catalogadas, das quais mais de 220 possuem usos medicinais. Espécies da família Myrtaceae são usadas na medicina popular em distúrbios gastrointestinais, estados hemorrágicos e doenças infecciosas, muitas são conhecidas pela presença de óleos essenciais. O objetivo deste trabalho foi realizar o estudo farmacognóstico das folhas e casca da espécie Myrcia tomentosa (Aubl.) DC. com o intuito de estabelecer parâmetros de qualidade; determinar a composição química do óleo essencial (folhas, casca e flores), bem como avaliar a ocorrência de variação sazonal entre os constituintes químicos do óleo essencial das folhas; e avaliar a atividade antimicrobiana do óleo essencial das folhas, do extrato etanólico bruto e frações das folhas e casca. Para tanto, parte dos materiais botânicos foi utilizada nas análises macro e microscópicas e outra parte nas análises fitoquímicas e nos doseamentos de fenóis totais, taninos e de flavonóides. A Cromatografia Gasosa acoplada à Espectrometria de Massas (CG/EM) foi utilizada para a caracterização química dos óleos essenciais e o teste da microdiluição em caldo para a avaliação da atividade antimicrobiana e determinação da Concentração Inibitória Mínima (CIM). No estudo morfo-anatômico das folhas verificou-se que são simples, coriáceas e predominantemente opostas cruzadas. Microscopicamente são hipoestomáticas, apresentam numerosos tricomas tectores unicelulares, têm cavidades secretoras e idioblastos com cristais prismáticos. A casca possui forma plana, às vezes canaletada e encurvada. Em nível microscópico apresenta cristais prismáticos, esclereides e a periderme é formada nas camadas mais internas. Na triagem fitoquímica foram detectados óleo essencial, taninos e terpenos, tanto nas folhas como na casca, flavonóides apenas nas folhas e saponina apenas na casca. Na análise dos óleos essenciais o (2E,6E)-Metil farnesoato foi o componente majoritário nas flores e casca, enquanto as folhas demonstraram variação sazonal na sua composição química, com Biciclogermacreno e (2E,6E)-Metil farnesoato fazendo parte dos componentes majoritários. Na avaliação antimicrobiana o óleo essencial das folhas demonstrou atividade para Micrococcus roseus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus e Staphylococcus aureus (CIM = 125 250 μg/mL) e para os fungos Cryptococcus neoformans e Cryptococcus gatti (CIM = 62,5 500 μg/mL); as frações tiveram atividade moderada para a maioria das bactérias Gram-positivas, sendo que as frações hexano e diclorometano das folhas, e acetato de etila e aquosa da casca foram as mais ativas (CIM = 250 500 μg/mL); com relação à atividade antifúngica, as frações testadas apresentaram atividade boa ou moderada para Candida sp (CIM = 15,62 125 μg/mL) e as frações hexano e diclorometano das folhas mostraram boa atividade para Cryptococcus sp (CIM = 15,62 62,50 μg/mL). Os resultados obtidos contribuem para a identificação da espécie M. tomentosa e demonstram o seu potencial para o tratamento de doenças fúngicas.Made available in DSpace on 2014-07-29T16:11:46Z (GMT). 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dc.title.por.fl_str_mv |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
dc.title.alternative.eng.fl_str_mv |
Morphology, anatomy, chemical composition of essential oil and antimicrobial activity of the leaves and stem bark of Myrcia tomentosa (Aubl.) DC. |
title |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
spellingShingle |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. SÁ, Fabyola Amaral da Silva Myrtaceae Goiaba-brava Atividade antifúngica Plantas medicinais 1.Morfoanatomia 2.Óleo essencial 3.Atividade antimicrobiana 4.Plantas medicinais Myrtaceae Goiaba-brava Antifungal activity Medicinal plants CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
title_full |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
title_fullStr |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
title_full_unstemmed |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
title_sort |
Morfoanatomia, composição química do óleo essencial e atividade antimicrobiana das folhas e casca do caule de Myrcia tomentosa (Aubl.) DC. |
author |
SÁ, Fabyola Amaral da Silva |
author_facet |
SÁ, Fabyola Amaral da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
PAULA, José Realino de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3191837532986128 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7688986744408368 |
dc.contributor.author.fl_str_mv |
SÁ, Fabyola Amaral da Silva |
contributor_str_mv |
PAULA, José Realino de |
dc.subject.por.fl_str_mv |
Myrtaceae Goiaba-brava Atividade antifúngica Plantas medicinais 1.Morfoanatomia 2.Óleo essencial 3.Atividade antimicrobiana 4.Plantas medicinais |
topic |
Myrtaceae Goiaba-brava Atividade antifúngica Plantas medicinais 1.Morfoanatomia 2.Óleo essencial 3.Atividade antimicrobiana 4.Plantas medicinais Myrtaceae Goiaba-brava Antifungal activity Medicinal plants CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Myrtaceae Goiaba-brava Antifungal activity Medicinal plants |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
The Brazilian Savanna is recognized as the richest in the world, sheltering more than 6.500 plant species already cataloged, and medicinal uses are attributed to over 220. Species of Myrtaceae family are used in folk medicine in gastrointestinal disorders, infectious diseases and hemorrhagic states, many are known by the presence of essential oils. The objective of this work was the pharmacognostic study of leaves and bark of species Myrcia tomentosa (Aubl.) DC. in order to establish quality parameters and to determine the chemical composition of essential oil (leaves, bark and flowers), as well as to assess the occurrence of seasonal variation between the chemical constituents of essential oil from leaves and to evaluate the antimicrobial activity of leaf essential oil, ethanolic crude extract and fractions from leaf and bark extracts. For this purpose, part of the botanical materials was used in microscopic analysis and in phytochemical analysis. The contents of total phenols, tannins and of total flavonoids were evaluated. The gas chromatography coupled to mass spectrometry (GC / MS) was used to characterize the chemistry of essential oils and the broth microdilution test was used for both evaluation of antimicrobial activity and determination of Minimum Inhibitory Concentration (MIC). In morpho-anatomical study of leaves, it was found that they are simple, leathery and predominantly opposing crossed. Microscopically are hypostomatic with numerous unicellular trichomes, have secretory cavities and idioblasts with prismatic crystals. The bark has flat, curved and sometimes channeled shape. At microscopic level, the bark shows prismatic crystals, sclereids and periderm is formed in the inner layers. In the phytochemical screening, it was found essential oils, tannins and terpenes in leaves and bark, only flavonoids in leaves and saponin in bark. In the essential oils analysis, it was found that (2E, 6E)-Methyl farnesoate is the major component in the flowers and bark essential oils, while the leaves essential oil showed seasonal variation in their chemical composition, with Bicyclogermacrene and (2E, 6E)-Methyl farnesoate as major components The antimicrobial evaluation of leaf essential oil showed activity for Micrococcus roseus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and Staphylococcus aureus (MIC = 125 - 250 μg/mL) and the fungi Cryptococcus neoformans and Cryptococcus gattii (MIC = 62,5 500 μg/mL); the fractions were moderate activity for most Gram-positive bacteria, and the hexane and dichlorometane fractions of the leaves, and ethyl acetate and aqueous bark were most active (MIC = 250 500 μg/mL). With respect to antifungal activity, the fractions tested showed good or moderate activity for Candida sp (MIC = 15,62 125 μg/mL) and hexanic and dichloromethanic fractions of leaves showed good activity for Cryptococcus sp (MIC = 15,62 62,50 μg/mL). The results contribute to the identification of the species M. tomentosa and demontrate its potential for the treatment of fungal diseases. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-11-05 |
dc.date.available.fl_str_mv |
2011-01-26 |
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2014-07-29T16:11:46Z |
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SÁ, Fabyola Amaral da Silva. Morphology, anatomy, chemical composition of essential oil and antimicrobial activity of the leaves and stem bark of Myrcia tomentosa (Aubl.) DC.. 2010. 27 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/2093 |
dc.identifier.dark.fl_str_mv |
ark:/38995/00130000091g8 |
identifier_str_mv |
SÁ, Fabyola Amaral da Silva. Morphology, anatomy, chemical composition of essential oil and antimicrobial activity of the leaves and stem bark of Myrcia tomentosa (Aubl.) DC.. 2010. 27 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2010. ark:/38995/00130000091g8 |
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http://repositorio.bc.ufg.br/tede/handle/tde/2093 |
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Universidade Federal de Goiás |
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UFG |
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BR |
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Ciências da Saúde - Farmácia |
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Universidade Federal de Goiás |
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