Efeitos dos extratos de folhas de campomanesia adamantium e hymenaea martiana hayne sobre células de osteossarcoma canino e células endoteliais humanas

• Ano de defesa: 2020
• Autor(a) principal: Vieira, Vanessa de Souza
• Orientador(a): Araújo, Eugênio Gonçalves de
• Banca de defesa: Araújo, Eugênio Gonçalves, Pfrimer, Gabriel de Abreu, Pereira, Kleber Fernando, Arnhold, Emmanuel, Oliveira, Gerlon de Almeida Ribeiro de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: Tumores ósseos; HUVEC; Cultivo celular; Gabirobeira; Jatobá
• Palavras-chave em Inglês: Bone tumors; HUVEC; Cell culture; Gabirobeira; Jatoba
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/10708

Osteosarcoma (OSA) is a very aggressive tumor in dogs, with low survival rate and ineffective treatment. In the search for alternative sources of therapy, the Brazilian biome becomes a scientific hope, presenting a diversity of medicinal plants of popular knowledge and use. However, they can have controversial biological functions. The aim of this study was to verify the bioactivity of the leaves of Campomania adamantium and Hymenaea martiana Hayne on canine osteosarcoma (OC) cells from cell cultures and human umbilical vein endothelial cells (HUVEC). As cells were cultured and subjected to treatment with C. adamantium (1 μg / mL, 10 μg / mL, 100 μg / mL, 1000 μg / mL) and H. martiana Hayne (10μL / mL, 100μL / mL, 1000μL / mL , 2000μL / mL and 5000μL / mL), in the 24h, 48h and 72h exposure periods in normoxia and 24h in cells exposed to oxidative stress, induced by hydrogen peroxide. The results were analyzed by analyzing cell viability and cytotoxicity using the tetrazolium reduction method (MTT). This study brings unprecedented results in relation to extractive effects, as it was possible to demonstrate that they have little cytotoxic action. In addition, it was observed that the higher the dosage and the longer the exposure time, the greater the proliferative activity, with increased cell viability in the 72-hour group. In endothelial cells, similarly, there was no cytotoxic activity, in addition to the occurrence of increased cell viability, even after submission to oxidative stress. C. adamantium leaf extract increased viability and did not show cytotoxic action in canine osteosarcoma cells and endothelial cells under oxidative stress. The crude ethanolic extract of the leaves of Hymenaea martiana Hayne, increased the cell viability of canine osteosarcoma cells and human umbilical vein endothelial cells, submitted to oxidative stress.