Micropropagação calogênese e irradiação da figueira "Roxo de valinhos"

• Ano de defesa: 2006
• Autor(a) principal: Ferreira, Ester Alice
• Orientador(a): Pasqual, Moacir
• Banca de defesa: Luz, José Magno Queiroz, Ramos, José Darlan, Chalfun, Nilton Nagib Jorge, Alvarenga, Ângelo Alberico
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Lavras
• Programa de Pós-Graduação: Programa de Pós-Graduação em Agronomia/Fitotecnia
• Departamento: Departamento de Agricultura
• País: BRASIL
• Palavras-chave em Português: Reguladores de crescimento; Calogênese; Mutação; Radiação gama; Growth regulator; Callogenesis; Mutation; Gamma radiation
• Área do conhecimento CNPq: Ciências Agrárias
• Link de acesso: https://repositorio.ufla.br/handle/1/3112

It was carried out three in vitro works with ´Roxo de Valinhos´ fig plants. The first one aimed to verify the sensibility of explants to gamma radiation and the plantlets were separated by size (2,5 to 4,5; 5 to 7 and 8 to 10 cm) and were taken to the Nuclear Energy Center, irradiated on the doses 10, 20,30,40 e 50 Gy and cutted separating the bud portion: apical, medium and base. It was evaluated 90 days after inoculation by mortality, anomalies, number of leaves and length of aerial part. On the second work, it was evaluated the efficiency of nodal and leaves explants, the effects of light and dark and the best combination of 2,4D and kinetin at 0; 0,5; 1,0; 2,0 e 4,0 mgL-1 in the in vitro callogenesis. The characteristics evaluated were: growing intensity and weight of fresh mass of calluses. The third work aimed to optimize the micropropagation protocol testing: 1) alteration on WPM concentration - 0, 50,100,150 e 200% and the sucrose - 0,10,20 e 40g; 2) inoculation of nodal explants with 1,2,3 and 4 buds in combination with different doses of kinetin at 0; 0,5;1,0; 2,0 e 4,0 mgL-1.The evaluation were carried out 90 days after the inoculation by: root and aerial part high; weight of root and aerial part. The results revealed that doses until 50 Gy do not cause death of plantlets; doses over 30Gy block root formation, plantlets size 2,5 to 5 cm irradiated at 10 Gy present higher lengths of aerial part and number of buds and the increase of irradiated doses caused reduction on the weight of plantlets in any size of plantlets. The callogenesis on fig plants ´Roxo de Valinhos´ can be obtained by leaves explants, absence of light and presence of 2,4D and kinetin at 4 mgL-1. On the sprouts multiplication can be taken 100% of WPM medium added with 10gL-1 of sucrose. When it is wished elongated sprout the use of medium without kinetin and explants with 1 or 2 buds promote best results. Adding 0,5 mgL-1 of kinetin and taking explants with 3 buds promote larger sprouts number.