Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR

Detalhes bibliográficos
Ano de defesa: 2000
Autor(a) principal: Ana Paula Reinato Stynen
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Equino Doenças
Infecções por campylobacter
Bovino
Lavado prepucial
Diagnóstico
Gene 23S rDNA
Campylobacter fetus
Campilobacteriose genital bovina
PCR
Link de acesso: https://hdl.handle.net/1843/BUDB-8BQGN8
Resumo: A PCR based on the 23S rDNA sequence was developed for the detection of C. fetus from preputial washings of bulls. Three extraction methods were evaluated for the extraction of DNA in preputial washings: the guanidiniu, tiocynate, the alkaline lysis and the Chelex 100 methods. To determine the specificity of the test, Camppylobacter sp, Helicobacter sp and Escherichia coli reference strains were submitted to PCR. Preputial washings of 47 bulls from one farm with a positive diagnosis of Bovine Genital Campilobactosis were tested by PCR and direct fluorescent antibody test (DFAT). Only C. fetus subps, fetus and C.fetus subps, venerealis were amplified by PCR and the detection limit was 3,8 x 10² CFU/ml of C. fetus. The specificity of the amplified product was confirmed by restriction analysis with Avall. Eight bulls were considered infected by the two tecniques, but three animals were only positive by PCR and seven only by DFAT. The present PCR assay was suitable to detect C. fetus in preputial washing samples.
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spelling Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCREquino DoençasInfecções por campylobacterBovinoLavado prepucialDiagnósticoGene 23S rDNACampylobacter fetusCampilobacteriose genital bovinaPCRA PCR based on the 23S rDNA sequence was developed for the detection of C. fetus from preputial washings of bulls. Three extraction methods were evaluated for the extraction of DNA in preputial washings: the guanidiniu, tiocynate, the alkaline lysis and the Chelex 100 methods. To determine the specificity of the test, Camppylobacter sp, Helicobacter sp and Escherichia coli reference strains were submitted to PCR. Preputial washings of 47 bulls from one farm with a positive diagnosis of Bovine Genital Campilobactosis were tested by PCR and direct fluorescent antibody test (DFAT). Only C. fetus subps, fetus and C.fetus subps, venerealis were amplified by PCR and the detection limit was 3,8 x 10² CFU/ml of C. fetus. The specificity of the amplified product was confirmed by restriction analysis with Avall. Eight bulls were considered infected by the two tecniques, but three animals were only positive by PCR and seven only by DFAT. The present PCR assay was suitable to detect C. fetus in preputial washing samples.Universidade Federal de Minas Gerais2019-08-12T11:48:09Z2025-09-09T00:43:04Z2019-08-12T11:48:09Z2000-02-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/1843/BUDB-8BQGN8Ana Paula Reinato Styneninfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2025-09-09T00:43:04Zoai:repositorio.ufmg.br:1843/BUDB-8BQGN8Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-09T00:43:04Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
title Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
spellingShingle Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
Ana Paula Reinato Stynen
Equino Doenças
Infecções por campylobacter
Bovino
Lavado prepucial
Diagnóstico
Gene 23S rDNA
Campylobacter fetus
Campilobacteriose genital bovina
PCR
title_short Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
title_full Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
title_fullStr Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
title_full_unstemmed Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
title_sort Detecção de Campylobacter fetus em lavados prepuciais de touros pela PCR
author Ana Paula Reinato Stynen
author_facet Ana Paula Reinato Stynen
author_role author
dc.contributor.author.fl_str_mv Ana Paula Reinato Stynen
dc.subject.por.fl_str_mv Equino Doenças
Infecções por campylobacter
Bovino
Lavado prepucial
Diagnóstico
Gene 23S rDNA
Campylobacter fetus
Campilobacteriose genital bovina
PCR
topic Equino Doenças
Infecções por campylobacter
Bovino
Lavado prepucial
Diagnóstico
Gene 23S rDNA
Campylobacter fetus
Campilobacteriose genital bovina
PCR
description A PCR based on the 23S rDNA sequence was developed for the detection of C. fetus from preputial washings of bulls. Three extraction methods were evaluated for the extraction of DNA in preputial washings: the guanidiniu, tiocynate, the alkaline lysis and the Chelex 100 methods. To determine the specificity of the test, Camppylobacter sp, Helicobacter sp and Escherichia coli reference strains were submitted to PCR. Preputial washings of 47 bulls from one farm with a positive diagnosis of Bovine Genital Campilobactosis were tested by PCR and direct fluorescent antibody test (DFAT). Only C. fetus subps, fetus and C.fetus subps, venerealis were amplified by PCR and the detection limit was 3,8 x 10² CFU/ml of C. fetus. The specificity of the amplified product was confirmed by restriction analysis with Avall. Eight bulls were considered infected by the two tecniques, but three animals were only positive by PCR and seven only by DFAT. The present PCR assay was suitable to detect C. fetus in preputial washing samples.
publishDate 2000
dc.date.none.fl_str_mv 2000-02-18
2019-08-12T11:48:09Z
2019-08-12T11:48:09Z
2025-09-09T00:43:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1843/BUDB-8BQGN8
url https://hdl.handle.net/1843/BUDB-8BQGN8
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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