Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Mariana Lázaro Sales
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Tuberculose humana
Micobacterias Identificação
Tuberculose em bovino
Reação em cadeia de polimerase
Marcadores moleculares
M bovis
M tuberculosis
Tuberculose
PCR
Link de acesso: https://hdl.handle.net/1843/SMOC-9L2PT7
Resumo: Mycobacterium bovis and Mycobacterium tuberculosis are the causative agents of bovine and human tuberculosis, respectively. The standard diagnosis is based on the isolation and identification of bacterial colonies through biochemical methods. These methodologies in addition to being costly, require an environment with high level of biosecurity and a great time of cultivation and tests to obtain the results. The molecular tests based on the principles of real-time PCR using the fluorophore Eva Green were efficient in the identification of M. bovis and M. tuberculosis. In addition, analyses were performed with primers and molecular markers, described in the literature, able to differentiate the M. bovis of M. tuberculosis by PCR. The results showed that some of the molecular markers are found in both microorganisms. The work of this dissertation has enabled the development of real-time PCR techniques able to identify colonies of M. bovis and M. tuberculosis.
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spelling Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCRTuberculose humanaMicobacterias IdentificaçãoTuberculose em bovinoReação em cadeia de polimeraseMarcadores molecularesM bovisM tuberculosisTuberculosePCRMycobacterium bovis and Mycobacterium tuberculosis are the causative agents of bovine and human tuberculosis, respectively. The standard diagnosis is based on the isolation and identification of bacterial colonies through biochemical methods. These methodologies in addition to being costly, require an environment with high level of biosecurity and a great time of cultivation and tests to obtain the results. The molecular tests based on the principles of real-time PCR using the fluorophore Eva Green were efficient in the identification of M. bovis and M. tuberculosis. In addition, analyses were performed with primers and molecular markers, described in the literature, able to differentiate the M. bovis of M. tuberculosis by PCR. The results showed that some of the molecular markers are found in both microorganisms. The work of this dissertation has enabled the development of real-time PCR techniques able to identify colonies of M. bovis and M. tuberculosis.Universidade Federal de Minas Gerais2019-08-10T12:21:36Z2025-09-09T00:25:01Z2019-08-10T12:21:36Z2012-05-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/1843/SMOC-9L2PT7Mariana Lázaro Salesinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2025-09-09T00:25:01Zoai:repositorio.ufmg.br:1843/SMOC-9L2PT7Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-09T00:25:01Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
title Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
spellingShingle Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
Mariana Lázaro Sales
Tuberculose humana
Micobacterias Identificação
Tuberculose em bovino
Reação em cadeia de polimerase
Marcadores moleculares
M bovis
M tuberculosis
Tuberculose
PCR
title_short Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
title_full Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
title_fullStr Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
title_full_unstemmed Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
title_sort Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR
author Mariana Lázaro Sales
author_facet Mariana Lázaro Sales
author_role author
dc.contributor.author.fl_str_mv Mariana Lázaro Sales
dc.subject.por.fl_str_mv Tuberculose humana
Micobacterias Identificação
Tuberculose em bovino
Reação em cadeia de polimerase
Marcadores moleculares
M bovis
M tuberculosis
Tuberculose
PCR
topic Tuberculose humana
Micobacterias Identificação
Tuberculose em bovino
Reação em cadeia de polimerase
Marcadores moleculares
M bovis
M tuberculosis
Tuberculose
PCR
description Mycobacterium bovis and Mycobacterium tuberculosis are the causative agents of bovine and human tuberculosis, respectively. The standard diagnosis is based on the isolation and identification of bacterial colonies through biochemical methods. These methodologies in addition to being costly, require an environment with high level of biosecurity and a great time of cultivation and tests to obtain the results. The molecular tests based on the principles of real-time PCR using the fluorophore Eva Green were efficient in the identification of M. bovis and M. tuberculosis. In addition, analyses were performed with primers and molecular markers, described in the literature, able to differentiate the M. bovis of M. tuberculosis by PCR. The results showed that some of the molecular markers are found in both microorganisms. The work of this dissertation has enabled the development of real-time PCR techniques able to identify colonies of M. bovis and M. tuberculosis.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-25
2019-08-10T12:21:36Z
2019-08-10T12:21:36Z
2025-09-09T00:25:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1843/SMOC-9L2PT7
url https://hdl.handle.net/1843/SMOC-9L2PT7
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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