Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Denis Alexis Molina Molina
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Epitopos de Linfócito B
Crotalus cascavella
Crotoxina
Fosfolipase A2
Venenos de serpentes
Bioquímica e imunologia
Crotalus durissus terrificus
Epitopos
Peptídeos sintéticos
Fosfolipasa A2 (PLA2)
Link de acesso: https://hdl.handle.net/1843/BUBD-AKBQUW
Resumo: Crotoxin (CTx) is a dimeric protein that is responsible for the neurotoxic activity of Crotalus durissus terrificus venom. In this work we have used 3 different techniques for mapping of linear epitopes of the Crotoxin B cells. The three methods were: Prediction by bioinformatics, Spot-Synthesis method and mapping by subcutaneous implants of peptides in mice. Sera of horses immunized with the total venom of C. d. terrificus were used in the Spot-Synthesis method. Immunoassays in the Spot-Synthesis method have shown that the C-terminal region of each of the CTx subunits is a dominant immunogenic region. These results were similar to the ones obtained by the bioinformatic method. Mapping by subcutaneous implants has identified other immunodominant regions of the crotoxin. The previously identified amino acid sequences corresponding to the immunogenic regions were chemically synthesized and the purity of these peptides were evaluated by mass spectrometry. The synthetic peptides were antigenically characterized by immunoblot using anticrotalic horse serum, for the immunogenic characterization of peptides, they were polymerized with glutaraldehyde and used as immunogens in the immunization of mice. Anti-peptide anti-mouse antibodies recognized CTx, the total venom of C. d. Terrificus and the synthetic peptides.All epitopes were found in the CTx 3D structure and they are located on the surface of the protein
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spelling Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)Epitopos de Linfócito BCrotalus cascavellaCrotoxinaFosfolipase A2Venenos de serpentesBioquímica e imunologiaCrotalus durissus terrificusEpitoposCrotoxinaPeptídeos sintéticosFosfolipasa A2 (PLA2)Crotoxin (CTx) is a dimeric protein that is responsible for the neurotoxic activity of Crotalus durissus terrificus venom. In this work we have used 3 different techniques for mapping of linear epitopes of the Crotoxin B cells. The three methods were: Prediction by bioinformatics, Spot-Synthesis method and mapping by subcutaneous implants of peptides in mice. Sera of horses immunized with the total venom of C. d. terrificus were used in the Spot-Synthesis method. Immunoassays in the Spot-Synthesis method have shown that the C-terminal region of each of the CTx subunits is a dominant immunogenic region. These results were similar to the ones obtained by the bioinformatic method. Mapping by subcutaneous implants has identified other immunodominant regions of the crotoxin. The previously identified amino acid sequences corresponding to the immunogenic regions were chemically synthesized and the purity of these peptides were evaluated by mass spectrometry. The synthetic peptides were antigenically characterized by immunoblot using anticrotalic horse serum, for the immunogenic characterization of peptides, they were polymerized with glutaraldehyde and used as immunogens in the immunization of mice. Anti-peptide anti-mouse antibodies recognized CTx, the total venom of C. d. Terrificus and the synthetic peptides.All epitopes were found in the CTx 3D structure and they are located on the surface of the proteinUniversidade Federal de Minas Gerais2019-08-12T21:22:20Z2025-09-09T01:05:49Z2019-08-12T21:22:20Z2017-02-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/1843/BUBD-AKBQUWDenis Alexis Molina Molinainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2025-09-09T01:05:49Zoai:repositorio.ufmg.br:1843/BUBD-AKBQUWRepositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-09T01:05:49Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
title Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
spellingShingle Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
Denis Alexis Molina Molina
Epitopos de Linfócito B
Crotalus cascavella
Crotoxina
Fosfolipase A2
Venenos de serpentes
Bioquímica e imunologia
Crotalus durissus terrificus
Epitopos
Crotoxina
Peptídeos sintéticos
Fosfolipasa A2 (PLA2)
title_short Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
title_full Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
title_fullStr Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
title_full_unstemmed Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
title_sort Mapeamento de epítopos da crotoxina, principal neurotoxina do veneno de Crotalus durissus terrificus (cascavel sul americana)
author Denis Alexis Molina Molina
author_facet Denis Alexis Molina Molina
author_role author
dc.contributor.author.fl_str_mv Denis Alexis Molina Molina
dc.subject.por.fl_str_mv Epitopos de Linfócito B
Crotalus cascavella
Crotoxina
Fosfolipase A2
Venenos de serpentes
Bioquímica e imunologia
Crotalus durissus terrificus
Epitopos
Crotoxina
Peptídeos sintéticos
Fosfolipasa A2 (PLA2)
topic Epitopos de Linfócito B
Crotalus cascavella
Crotoxina
Fosfolipase A2
Venenos de serpentes
Bioquímica e imunologia
Crotalus durissus terrificus
Epitopos
Crotoxina
Peptídeos sintéticos
Fosfolipasa A2 (PLA2)
description Crotoxin (CTx) is a dimeric protein that is responsible for the neurotoxic activity of Crotalus durissus terrificus venom. In this work we have used 3 different techniques for mapping of linear epitopes of the Crotoxin B cells. The three methods were: Prediction by bioinformatics, Spot-Synthesis method and mapping by subcutaneous implants of peptides in mice. Sera of horses immunized with the total venom of C. d. terrificus were used in the Spot-Synthesis method. Immunoassays in the Spot-Synthesis method have shown that the C-terminal region of each of the CTx subunits is a dominant immunogenic region. These results were similar to the ones obtained by the bioinformatic method. Mapping by subcutaneous implants has identified other immunodominant regions of the crotoxin. The previously identified amino acid sequences corresponding to the immunogenic regions were chemically synthesized and the purity of these peptides were evaluated by mass spectrometry. The synthetic peptides were antigenically characterized by immunoblot using anticrotalic horse serum, for the immunogenic characterization of peptides, they were polymerized with glutaraldehyde and used as immunogens in the immunization of mice. Anti-peptide anti-mouse antibodies recognized CTx, the total venom of C. d. Terrificus and the synthetic peptides.All epitopes were found in the CTx 3D structure and they are located on the surface of the protein
publishDate 2017
dc.date.none.fl_str_mv 2017-02-09
2019-08-12T21:22:20Z
2019-08-12T21:22:20Z
2025-09-09T01:05:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1843/BUBD-AKBQUW
url https://hdl.handle.net/1843/BUBD-AKBQUW
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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