Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.

Detalhes bibliográficos
Ano de defesa: 2007
Autor(a) principal: Pedro Moreira Couto Motta
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Muar Doenças
Asinino Doenças
Anemia infecciosa equina
Equino Doenças
Teste imunoenzimático
Link de acesso: https://hdl.handle.net/1843/VETC-7AUMAS
Resumo: A lot of studies about horses (Equus caballus) immune responses to equine infectious anemia virus (EIAV) have been done all over the world, but there are little information concerning immune responses of mule (Equus caballus X Equus asinus) and donkey (Equus asinus) to natural infection with EIAV. The aim of this work was to compare the serological and virus detection in asinus, mules and equines naturally infected with EIAV, and evaluate the importance of mules and asinus to maintain the EIAV in the Brazilian herds. In the present study sera and peripheral blood mononuclear cells (PBMC) samples were collected of 124 horses, 119 mules and 75 asinus, in a total of 318 samples. DNA of PBMC was extracted from 114 horses, 79 mules and 35 asinus. All samples were tested by Agar Gel Immunodiffusion (AGID) and Enzyme-linked Immunosorbent Assay (ELISA). DNA was submitted to nested polymerase chain Reaction (nPCR). The correlation to AGID and ELISA for equines was better than mules. The same was observed between AGID and nPCR. When the correlation between ELISA and nPCR was compared, the best correlation was observed in equines again. The number of asinus was not significant to statistic evaluation but results showed that 10 negative asinus in AGID were positive in ELISA and 7 were positive in nPCR. Two positive asinus in AGID were positive in ELISA and nPCR. These results showed that serological responses and virus detection in mules might be different from the responses in equines. The concordance between AGID, ELISA and PCR tests were better in equine than in mules.
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spelling 2019-08-11T12:03:52Z2025-09-08T22:50:45Z2019-08-11T12:03:52Z2007-02-16https://hdl.handle.net/1843/VETC-7AUMASA lot of studies about horses (Equus caballus) immune responses to equine infectious anemia virus (EIAV) have been done all over the world, but there are little information concerning immune responses of mule (Equus caballus X Equus asinus) and donkey (Equus asinus) to natural infection with EIAV. The aim of this work was to compare the serological and virus detection in asinus, mules and equines naturally infected with EIAV, and evaluate the importance of mules and asinus to maintain the EIAV in the Brazilian herds. In the present study sera and peripheral blood mononuclear cells (PBMC) samples were collected of 124 horses, 119 mules and 75 asinus, in a total of 318 samples. DNA of PBMC was extracted from 114 horses, 79 mules and 35 asinus. All samples were tested by Agar Gel Immunodiffusion (AGID) and Enzyme-linked Immunosorbent Assay (ELISA). DNA was submitted to nested polymerase chain Reaction (nPCR). The correlation to AGID and ELISA for equines was better than mules. The same was observed between AGID and nPCR. When the correlation between ELISA and nPCR was compared, the best correlation was observed in equines again. The number of asinus was not significant to statistic evaluation but results showed that 10 negative asinus in AGID were positive in ELISA and 7 were positive in nPCR. Two positive asinus in AGID were positive in ELISA and nPCR. These results showed that serological responses and virus detection in mules might be different from the responses in equines. The concordance between AGID, ELISA and PCR tests were better in equine than in mules.Universidade Federal de Minas GeraisEqüinoDiagnósticoAIEMuarAsininoMuar DoençasAsinino DoençasAnemia infecciosa equinaEquino DoençasTeste imunoenzimáticoComparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPedro Moreira Couto Mottainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGRomulo Cerqueira LeiteMarcelo Fernandes CamargosJenner Karlisson Pimenta dos ReisMuitos estudos têm sido realizados em todo o mundo a respeito da resposta imunológica de cavalos (Equus caballus) à Anemia Infecciosa Eqüina (AIE), no entanto existe pouca informação a respeito da resposta imunológica de muares (Equus caballus X Equus asinus) e asininos (Equus asinus) naturalmente infectados pelo VAIE. O objetivo desse trabalho foi comparar o perfil sorológico e a detecção do vírus da AIE em asininos, muares e eqüinos naturalmente infectados e avaliar a importância destas espécies como mantenedoras da AIE nos rebanhos brasileiros. No presente estudo, foi coletado sangue para obtenção de soro e células mononucleares periféricas (PBMC) de 124 cavalos, 119 muares e 75 asininos, perfazendo um total de 318 animais. Foi extraído DNA do PBMC de 114 cavalos, 79 muares e 35 asininos. Todas as amostras foram testadas através da Imunodifusão em Gel de Agar (IDGA) e Ensaio imunoenzimatico (ELISA). O DNA foi submetido à reação nested da polimerase em cadeia (nPCR). Nos eqüinos ocorreu a melhor correlação entre IDGA e ELISA do que nos muares. O mesmo foi observado entre a IDGA e a nPCR. Quando se comparou a correlação entre ELISA e a nPCR, o melhor resultado foi observado para amostras de eqüinos novamente. O número de amostras de asininos foi insuficiente para se fazer uma análise estatística, mas os resultados mostraram que dez asininos, negativos na IDGA, foram positivos no ELISA e sete positivas na nPCR. As duas amostras positivas na IDGA foram também positivas no ELISA e na nPCR. De acordo com os resultados obtidos, pode-se concluir que as respostas sorológicas e viremia dos muares são diferentes das respostas dos eqüinos. A concordância entre a IDGA, ELISA e PCR em amostras de eqüinos foi superior aos resultados obtidos em amostras de muares.UFMGORIGINALdisserta__o_pedro_moreira_couto_motta.pdfapplication/pdf288583https://repositorio.ufmg.br//bitstreams/710e2fda-a0a0-4884-9a8a-150145fa5259/download4979270e4c02f12e5c9e5f93a76438f0MD51trueAnonymousREADTEXTdisserta__o_pedro_moreira_couto_motta.pdf.txttext/plain73600https://repositorio.ufmg.br//bitstreams/d870fa51-d216-46c5-a1b0-7e8b9a158a03/download74cf3ea9b5758ba57ae04d3909d07177MD52falseAnonymousREAD1843/VETC-7AUMAS2025-09-08 19:50:45.767open.accessoai:repositorio.ufmg.br:1843/VETC-7AUMAShttps://repositorio.ufmg.br/Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-08T22:50:45Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
title Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
spellingShingle Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
Pedro Moreira Couto Motta
Muar Doenças
Asinino Doenças
Anemia infecciosa equina
Equino Doenças
Teste imunoenzimático
Eqüino
Diagnóstico
AIE
Muar
Asinino
title_short Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
title_full Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
title_fullStr Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
title_full_unstemmed Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
title_sort Comparação da IDGA, ELISA e nested PCR no diagnóstico da anemia infecciosa eqüina em eqüinos, Asininos e Muares.
author Pedro Moreira Couto Motta
author_facet Pedro Moreira Couto Motta
author_role author
dc.contributor.author.fl_str_mv Pedro Moreira Couto Motta
dc.subject.por.fl_str_mv Muar Doenças
Asinino Doenças
Anemia infecciosa equina
Equino Doenças
Teste imunoenzimático
topic Muar Doenças
Asinino Doenças
Anemia infecciosa equina
Equino Doenças
Teste imunoenzimático
Eqüino
Diagnóstico
AIE
Muar
Asinino
dc.subject.other.none.fl_str_mv Eqüino
Diagnóstico
AIE
Muar
Asinino
description A lot of studies about horses (Equus caballus) immune responses to equine infectious anemia virus (EIAV) have been done all over the world, but there are little information concerning immune responses of mule (Equus caballus X Equus asinus) and donkey (Equus asinus) to natural infection with EIAV. The aim of this work was to compare the serological and virus detection in asinus, mules and equines naturally infected with EIAV, and evaluate the importance of mules and asinus to maintain the EIAV in the Brazilian herds. In the present study sera and peripheral blood mononuclear cells (PBMC) samples were collected of 124 horses, 119 mules and 75 asinus, in a total of 318 samples. DNA of PBMC was extracted from 114 horses, 79 mules and 35 asinus. All samples were tested by Agar Gel Immunodiffusion (AGID) and Enzyme-linked Immunosorbent Assay (ELISA). DNA was submitted to nested polymerase chain Reaction (nPCR). The correlation to AGID and ELISA for equines was better than mules. The same was observed between AGID and nPCR. When the correlation between ELISA and nPCR was compared, the best correlation was observed in equines again. The number of asinus was not significant to statistic evaluation but results showed that 10 negative asinus in AGID were positive in ELISA and 7 were positive in nPCR. Two positive asinus in AGID were positive in ELISA and nPCR. These results showed that serological responses and virus detection in mules might be different from the responses in equines. The concordance between AGID, ELISA and PCR tests were better in equine than in mules.
publishDate 2007
dc.date.issued.fl_str_mv 2007-02-16
dc.date.accessioned.fl_str_mv 2019-08-11T12:03:52Z
2025-09-08T22:50:45Z
dc.date.available.fl_str_mv 2019-08-11T12:03:52Z
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