Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico
Ano de defesa: | 2014 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Minas Gerais
UFMG |
Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Link de acesso: | http://hdl.handle.net/1843/BUOS-9Q5J3W |
Resumo: | Placental release after birth involves loss of maternal-fetal adhesion and occurs only after complete maturation of the placentome, which is related to the decrease in cellularity of fetal and maternal tissues. Apoptosis is required for both the normal maturation and release of the placenta after birth. The aim of this study was to evaluate the occurrence of apoptosis in samples of placenta of cows in different stages of gestation. Samples of 15 healthy cows placentomes 4 (n = 5), 6 (n = 5) and 9 (n = 5) months of gestation were harvested and processed for routine histology, immunohistochemistry and histochemistry. The slides were stained with HE, Picrosirius Red and subjected to immunohistochemical analysis of proteins Caspase 3, Caspase 8, Bax, Bid and Bcl-2. Increase in number of vessels was not associated to increase in vascular area, during progression of gestation. The results of histomorphometry revealed increased labeling for Bax and Caspases 3 and 8 in trophoblastic binucleated cells in late pregnancy, awhile Bid remained without significant change. Histomorphometry analyzing the mononuclear trophoblast cells showed a high expression for Bax in early pregnancy, but decreased at 6 months of gestation. Immunolabeling revealed increased Caspases 3/8 and Bid with advancing of gestation. Further evaluation of type I and III collagens showed a decrease of both types of collagens at the end of gestation, which is very important to decrease maternal-fetal adhesion. The dissociation of the fibers of the stroma of chorionic villi decreased at 9 months of gestation, unlike the apoptotic index, which increased progressively with advancing gestation. These results confirm that Caspases 3 and 8 and Bax are involved in the mechanisms of activation of apoptosis through mitochondrial intrinsic and or extrinsic pathway during pregnancy in trophoblastic binucleated cells. In mononuclear trophoblast cells Bax loses importance in the apoptosis process, awhile Bid and caspases 3 and 8 become the most significant. |
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Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométricoCaspase 8ImunoistoquímicaBovinosCaspase 3BaxBidApoptoseMaturação placentáriaProliferação de célulasPlacenta/anatomia & histologiaProteína x associada a bcl-2PatologiaBovinosPlacenta/embriologiaOligonucleotídeosCaspasesApoptosePrenhezPlacental release after birth involves loss of maternal-fetal adhesion and occurs only after complete maturation of the placentome, which is related to the decrease in cellularity of fetal and maternal tissues. Apoptosis is required for both the normal maturation and release of the placenta after birth. The aim of this study was to evaluate the occurrence of apoptosis in samples of placenta of cows in different stages of gestation. Samples of 15 healthy cows placentomes 4 (n = 5), 6 (n = 5) and 9 (n = 5) months of gestation were harvested and processed for routine histology, immunohistochemistry and histochemistry. The slides were stained with HE, Picrosirius Red and subjected to immunohistochemical analysis of proteins Caspase 3, Caspase 8, Bax, Bid and Bcl-2. Increase in number of vessels was not associated to increase in vascular area, during progression of gestation. The results of histomorphometry revealed increased labeling for Bax and Caspases 3 and 8 in trophoblastic binucleated cells in late pregnancy, awhile Bid remained without significant change. Histomorphometry analyzing the mononuclear trophoblast cells showed a high expression for Bax in early pregnancy, but decreased at 6 months of gestation. Immunolabeling revealed increased Caspases 3/8 and Bid with advancing of gestation. Further evaluation of type I and III collagens showed a decrease of both types of collagens at the end of gestation, which is very important to decrease maternal-fetal adhesion. The dissociation of the fibers of the stroma of chorionic villi decreased at 9 months of gestation, unlike the apoptotic index, which increased progressively with advancing gestation. These results confirm that Caspases 3 and 8 and Bax are involved in the mechanisms of activation of apoptosis through mitochondrial intrinsic and or extrinsic pathway during pregnancy in trophoblastic binucleated cells. In mononuclear trophoblast cells Bax loses importance in the apoptosis process, awhile Bid and caspases 3 and 8 become the most significant.A liberação da placenta após o parto envolve a perda da adesão materno-fetal e ocorre somente após a maturação completa do placentoma, que está relacionada com a diminuição da celularidade dos tecidos fetal e materno. A apoptose é requerida tanto para a maturação quanto para a liberação normal da placenta após o parto. O objetivo do presente estudo foi avaliar a ocorrência de apoptose em amostras de placenta de vacas em diferentes fases de gestação. Amostras de placentomas de 15 vacas saudáveis com 4 (n=5), 6 (n=5) e 9 (n=5) meses de gestação foram colhidas e processadas rotineiramente para a histologia, imunoistoquímica e histoquímica. As lâminas obtidas foram coradas em HE, Picrosirius Red e submetidas à análise imunoistoquímica das proteínas Caspase 3, Caspase 8, Bax, Bid e Bcl-2. O aumento no número de vasos não necessariamente se associou ao aumento do calibre destes durante a evolução da gestação. Os resultados de histomorfometria revelaram aumento da marcação para Bax e Caspases 3 e 8 em células trofoblásticas binucleadas no final da gestação, enquanto o Bid se manteve sem alteração significativa. A histomorfometria das células trofoblásticas mononucleadas revelou expressão alta para Bax no início de gestação, com diminuição aos 6 meses de gestação e aumento das imunomarcações para Caspases 3 e 8, e Bid com o avanço gestacional. Os colágenos tipo I e III não aumentaram do terço médio ao final da gestação, o que é importante para a diminuição da adesão materno-fetal. A dissociação das fibras do estroma das vilosidades coriônicas diminuiu aos 9 meses de gestação, ao contrário do índice apoptótico que aumentou progressivamente com o avanço da gestação. Esses resultados confirmam que as Caspases 3 e 8, e o Bax estão envolvidos nos mecanismos de ativação da apoptose pela via intrínseca mitocondrial e/ou extrínseca ao longo da gestação em células trofoblásticas binucleadas, e que nas células trofoblásticas mononucleadas o Bax deixa de ser importante, enquanto o Bid e as Caspases 3 e 8 se tornam os mais significativos.Universidade Federal de Minas GeraisUFMGAnilton Cesar VasconcelosHumberto Eustáquio CoelhoMilene Alvarenga RachidPaula Peixoto Campos LopesTatiane Furtado de Carvalho2019-08-09T22:28:39Z2019-08-09T22:28:39Z2014-01-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/1843/BUOS-9Q5J3Winfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2019-11-14T07:53:54Zoai:repositorio.ufmg.br:1843/BUOS-9Q5J3WRepositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2019-11-14T07:53:54Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
title |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
spellingShingle |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico Tatiane Furtado de Carvalho Caspase 8 Imunoistoquímica Bovinos Caspase 3 Bax Bid Apoptose Maturação placentária Proliferação de células Placenta/anatomia & histologia Proteína x associada a bcl-2 Patologia Bovinos Placenta/embriologia Oligonucleotídeos Caspases Apoptose Prenhez |
title_short |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
title_full |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
title_fullStr |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
title_full_unstemmed |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
title_sort |
Apoptose e maturação placentária bovina: um estudo imunoistoquímico e morfométrico |
author |
Tatiane Furtado de Carvalho |
author_facet |
Tatiane Furtado de Carvalho |
author_role |
author |
dc.contributor.none.fl_str_mv |
Anilton Cesar Vasconcelos Humberto Eustáquio Coelho Milene Alvarenga Rachid Paula Peixoto Campos Lopes |
dc.contributor.author.fl_str_mv |
Tatiane Furtado de Carvalho |
dc.subject.por.fl_str_mv |
Caspase 8 Imunoistoquímica Bovinos Caspase 3 Bax Bid Apoptose Maturação placentária Proliferação de células Placenta/anatomia & histologia Proteína x associada a bcl-2 Patologia Bovinos Placenta/embriologia Oligonucleotídeos Caspases Apoptose Prenhez |
topic |
Caspase 8 Imunoistoquímica Bovinos Caspase 3 Bax Bid Apoptose Maturação placentária Proliferação de células Placenta/anatomia & histologia Proteína x associada a bcl-2 Patologia Bovinos Placenta/embriologia Oligonucleotídeos Caspases Apoptose Prenhez |
description |
Placental release after birth involves loss of maternal-fetal adhesion and occurs only after complete maturation of the placentome, which is related to the decrease in cellularity of fetal and maternal tissues. Apoptosis is required for both the normal maturation and release of the placenta after birth. The aim of this study was to evaluate the occurrence of apoptosis in samples of placenta of cows in different stages of gestation. Samples of 15 healthy cows placentomes 4 (n = 5), 6 (n = 5) and 9 (n = 5) months of gestation were harvested and processed for routine histology, immunohistochemistry and histochemistry. The slides were stained with HE, Picrosirius Red and subjected to immunohistochemical analysis of proteins Caspase 3, Caspase 8, Bax, Bid and Bcl-2. Increase in number of vessels was not associated to increase in vascular area, during progression of gestation. The results of histomorphometry revealed increased labeling for Bax and Caspases 3 and 8 in trophoblastic binucleated cells in late pregnancy, awhile Bid remained without significant change. Histomorphometry analyzing the mononuclear trophoblast cells showed a high expression for Bax in early pregnancy, but decreased at 6 months of gestation. Immunolabeling revealed increased Caspases 3/8 and Bid with advancing of gestation. Further evaluation of type I and III collagens showed a decrease of both types of collagens at the end of gestation, which is very important to decrease maternal-fetal adhesion. The dissociation of the fibers of the stroma of chorionic villi decreased at 9 months of gestation, unlike the apoptotic index, which increased progressively with advancing gestation. These results confirm that Caspases 3 and 8 and Bax are involved in the mechanisms of activation of apoptosis through mitochondrial intrinsic and or extrinsic pathway during pregnancy in trophoblastic binucleated cells. In mononuclear trophoblast cells Bax loses importance in the apoptosis process, awhile Bid and caspases 3 and 8 become the most significant. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-13 2019-08-09T22:28:39Z 2019-08-09T22:28:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/BUOS-9Q5J3W |
url |
http://hdl.handle.net/1843/BUOS-9Q5J3W |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais UFMG |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais UFMG |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
repositorio@ufmg.br |
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1823248565791621120 |