Resistência bacteriana às fluoroquinolonas em indígenas da Amazônia brasileira

• Ano de defesa: 2020
• Autor(a) principal: Vianello, Marco Aurélio
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Rio Grande do Norte; Brasil; UFRN; PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE
• Palavras-chave em Português: Hipervirulentas; Multirresistentes; Comunidade remota
• Link de acesso: https://repositorio.ufrn.br/handle/123456789/32469

Increased resistance to antimicrobials poses a threat to public health. Although the emergence of multidrug-resistant bacteria is closely related to the excessive and inappropriate use of antimicrobials, findings of potentially hypervirulent and multi-resistant bacteria in remote areas have been attracting the attention of scientific community. Thus, this work investigated the presence of resistant bacteria to fluoroquinolones in the gram-negative commensal microbiota of humans of different indigenous ethnic groups in the northwest of the Amazon Forest. Stool samples were collected from 36 indigenous individuals assisted by a health care center restricted to remote indigenous communities in the Amazon Forest. The identification of bacterial species was performed by MALDI-TOF and the susceptibility test to antimicrobials was assessed by the Kirby-Bauer technique and by automated methodology (Microscan-Beckman-Coulter). The detection of the resistome, virulome, plasmidome and MLST was performed by sequencing the complete genome, allowing the genotyping of the isolates. The analyzis carried out allowed the identification of international epidemic clones of E coli O8:H23‐B1‐ ST224, O15:H1‐D‐ST393, O1:H6-F-ST648 and O25:H4–B2- ST131, presenting a genetic profile of multiresistance and hypervirulence. This study reports for the first time in South America the clone O15: H1-D-ST393, representing a potential clone in early dispersion in this continent. These results emphasize the worrisome spread of multiresistant and hypervirulent bacteria in remote areas, theoretically with low exposure to antibiotics.