Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Rocha Neto, Pedro Carlos da
Orientador(a): Costa, Antônio de Lisboa Lopes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal do Rio Grande do Norte
Programa de Pós-Graduação: Programa de Pós-Graduação em Patologia Oral
Departamento: Odontologia
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://repositorio.ufrn.br/jspui/handle/123456789/17123
Resumo: Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-γ and TGF-β1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-γ and TGF-β1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-γ and TGF-β1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-γ between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-β1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-γ and TGF-β1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-β1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-γ and TGF-β1 between radicular and dentigerous cysts and that TGF-β1 was more significant than the IFN-γ in the capsule of dentigerous cysts
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spelling Rocha Neto, Pedro Carlos dahttp://lattes.cnpq.br/5529085665771149http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786083Z7&dataRevisao=nullMorais, Maria de Lourdes Silva Arrudahttp://lattes.cnpq.br/2371541508231170Galvão, Hébel Cavalcantihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9Costa, Antônio de Lisboa Lopes2014-12-17T15:32:21Z2012-11-222014-12-17T15:32:21Z2012-02-29ROCHA NETO, Pedro Carlos da. Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros. 2012. 81 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012.https://repositorio.ufrn.br/jspui/handle/123456789/17123Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-γ and TGF-β1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-γ and TGF-β1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-γ and TGF-β1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-γ between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-β1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-γ and TGF-β1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-β1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-γ and TGF-β1 between radicular and dentigerous cysts and that TGF-β1 was more significant than the IFN-γ in the capsule of dentigerous cystsOs cistos odontogênicos são cavidades patológicas revestidas por epitélio odontogênico e preenchidas por líquido, células descamadas, ou outros materiais. As lesões intra-ósseas, como o cisto radicular e o cisto dentígero, apresentam um potencial de expansão capaz de promover a destruição do tecido ósseo circunjacente. Os mecanismos relacionados a esse processo de expansão são a proliferação do epitélio cístico, o aumento da osmolaridade do fluido cístico e a síntese de fatores de reabsorção óssea como IFN-γ e TGF-β1. O objetivo deste estudo foi avaliar e comparar a expressão imuno-histoquímica do IFN-γ e do TGF-β1 entre cistos radiculares e cistos dentígeros com a finalidade de compreender o papel e o comportamento dessas proteínas no processo de expansão destes cistos. Selecionamos 20 casos de cisto radicular e 20 casos de cisto dentígero retirados dos arquivos do Laboratório de Patologia Oral da UFRN. Após análise dos dados clínicos, os casos foram submetidos a técnica de coloração de rotina (HE) e ao método imuno-histoquímico para evidenciação da expressão do IFN-γ e do TGF-β1 no epitélio e na cápsula dos referidos cistos. A análise estatística dos dados utilizando o teste de Mann-Whitney revelou que não houve diferença estatisticamente significativa da imunoexpressão do IFN- entre os epitélios (p=0,565) e cápsulas (p=0,414) dos cistos radiculares e cistos dentígeros. Além disso, não houve diferença estatisticamente significativa da imunoexpressão do TGF-β1 entre os epitélios (p=0,620) e cápsulas (p=0,056) dos cistos radiculares e cistos dentígeros. O teste de Wilcoxon revelou que não houve diferença estatisticamente significativa entre as imunoexpressões do IFN- e do TGF-β1 no epitélio (p=0,225) e na cápsula (p=0,370) dos cistos radiculares. Não houve diferença estatisticamente significativa entre as imunoexpressões do IFN- e do TGF-β1 no epitélio (p=0,361) dos cistos dentígeros. No entanto, houve diferença estatisticamente significativa entre as imunoexpressões do IFN- e do TGF-β1 na cápsula (p=0,001) dos cistos dentígeros, sendo o TGF-β1 o que apresentou a imunoexpressão mais significativa. Diante destes resultados, concluímos que não houve diferença de expressão imuno-histoquímica do IFN-γ e do TGF-β1 entre os cistos radiculares e dentígeros e que o TGF-β1 foi mais expressivo do que o IFN-γ na cápsula dos cistos dentígerosConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Patologia OralUFRNBROdontologiaCisto radicularCisto dentígeroImuno-histoquímicaInterferon gamaFatores de crescimento transformadoresRadicular cystDentigerous cystImmunohistochemistryInterferon gammaTransforming growth factorsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAExpressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígerosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALPedroCRN_DISSERT.pdfapplication/pdf3117630https://repositorio.ufrn.br/bitstream/123456789/17123/1/PedroCRN_DISSERT.pdf2544a3022fe67731388c2238d7dc5289MD51TEXTPedroCRN_DISSERT.pdf.txtPedroCRN_DISSERT.pdf.txtExtracted texttext/plain131868https://repositorio.ufrn.br/bitstream/123456789/17123/6/PedroCRN_DISSERT.pdf.txt6e7e9e9342465d1a06c047b1c6097833MD56THUMBNAILPedroCRN_DISSERT.pdf.jpgPedroCRN_DISSERT.pdf.jpgIM Thumbnailimage/jpeg3749https://repositorio.ufrn.br/bitstream/123456789/17123/7/PedroCRN_DISSERT.pdf.jpgafec12a356af839a2f836fc7d47b9b47MD57123456789/171232017-11-04 13:13:13.125oai:https://repositorio.ufrn.br:123456789/17123Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T16:13:13Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.por.fl_str_mv Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
title Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
spellingShingle Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
Rocha Neto, Pedro Carlos da
Cisto radicular
Cisto dentígero
Imuno-histoquímica
Interferon gama
Fatores de crescimento transformadores
Radicular cyst
Dentigerous cyst
Immunohistochemistry
Interferon gamma
Transforming growth factors
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
title_full Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
title_fullStr Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
title_full_unstemmed Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
title_sort Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
author Rocha Neto, Pedro Carlos da
author_facet Rocha Neto, Pedro Carlos da
author_role author
dc.contributor.authorID.por.fl_str_mv
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/5529085665771149
dc.contributor.advisorID.por.fl_str_mv
dc.contributor.advisorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786083Z7&dataRevisao=null
dc.contributor.referees1.pt_BR.fl_str_mv Morais, Maria de Lourdes Silva Arruda
dc.contributor.referees1ID.por.fl_str_mv
dc.contributor.referees1Lattes.por.fl_str_mv http://lattes.cnpq.br/2371541508231170
dc.contributor.referees2.pt_BR.fl_str_mv Galvão, Hébel Cavalcanti
dc.contributor.referees2ID.por.fl_str_mv
dc.contributor.referees2Lattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9
dc.contributor.author.fl_str_mv Rocha Neto, Pedro Carlos da
dc.contributor.advisor1.fl_str_mv Costa, Antônio de Lisboa Lopes
contributor_str_mv Costa, Antônio de Lisboa Lopes
dc.subject.por.fl_str_mv Cisto radicular
Cisto dentígero
Imuno-histoquímica
Interferon gama
Fatores de crescimento transformadores
topic Cisto radicular
Cisto dentígero
Imuno-histoquímica
Interferon gama
Fatores de crescimento transformadores
Radicular cyst
Dentigerous cyst
Immunohistochemistry
Interferon gamma
Transforming growth factors
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv Radicular cyst
Dentigerous cyst
Immunohistochemistry
Interferon gamma
Transforming growth factors
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-γ and TGF-β1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-γ and TGF-β1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-γ and TGF-β1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-γ between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-β1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-γ and TGF-β1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-β1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-γ and TGF-β1 between radicular and dentigerous cysts and that TGF-β1 was more significant than the IFN-γ in the capsule of dentigerous cysts
publishDate 2012
dc.date.available.fl_str_mv 2012-11-22
2014-12-17T15:32:21Z
dc.date.issued.fl_str_mv 2012-02-29
dc.date.accessioned.fl_str_mv 2014-12-17T15:32:21Z
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dc.identifier.citation.fl_str_mv ROCHA NETO, Pedro Carlos da. Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros. 2012. 81 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/17123
identifier_str_mv ROCHA NETO, Pedro Carlos da. Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros. 2012. 81 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012.
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