Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros
Ano de defesa: | 2012 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal do Rio Grande do Norte
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Patologia Oral
|
Departamento: |
Odontologia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufrn.br/jspui/handle/123456789/17123 |
Resumo: | Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-γ and TGF-β1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-γ and TGF-β1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-γ and TGF-β1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-γ between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-β1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-γ and TGF-β1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-β1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-γ and TGF-β1 between radicular and dentigerous cysts and that TGF-β1 was more significant than the IFN-γ in the capsule of dentigerous cysts |
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Rocha Neto, Pedro Carlos dahttp://lattes.cnpq.br/5529085665771149http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786083Z7&dataRevisao=nullMorais, Maria de Lourdes Silva Arrudahttp://lattes.cnpq.br/2371541508231170Galvão, Hébel Cavalcantihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9Costa, Antônio de Lisboa Lopes2014-12-17T15:32:21Z2012-11-222014-12-17T15:32:21Z2012-02-29ROCHA NETO, Pedro Carlos da. Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros. 2012. 81 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012.https://repositorio.ufrn.br/jspui/handle/123456789/17123Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-γ and TGF-β1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-γ and TGF-β1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-γ and TGF-β1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-γ between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-β1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-γ and TGF-β1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-β1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-γ and TGF-β1 between radicular and dentigerous cysts and that TGF-β1 was more significant than the IFN-γ in the capsule of dentigerous cystsOs cistos odontogênicos são cavidades patológicas revestidas por epitélio odontogênico e preenchidas por líquido, células descamadas, ou outros materiais. As lesões intra-ósseas, como o cisto radicular e o cisto dentígero, apresentam um potencial de expansão capaz de promover a destruição do tecido ósseo circunjacente. Os mecanismos relacionados a esse processo de expansão são a proliferação do epitélio cístico, o aumento da osmolaridade do fluido cístico e a síntese de fatores de reabsorção óssea como IFN-γ e TGF-β1. O objetivo deste estudo foi avaliar e comparar a expressão imuno-histoquímica do IFN-γ e do TGF-β1 entre cistos radiculares e cistos dentígeros com a finalidade de compreender o papel e o comportamento dessas proteínas no processo de expansão destes cistos. Selecionamos 20 casos de cisto radicular e 20 casos de cisto dentígero retirados dos arquivos do Laboratório de Patologia Oral da UFRN. Após análise dos dados clínicos, os casos foram submetidos a técnica de coloração de rotina (HE) e ao método imuno-histoquímico para evidenciação da expressão do IFN-γ e do TGF-β1 no epitélio e na cápsula dos referidos cistos. A análise estatística dos dados utilizando o teste de Mann-Whitney revelou que não houve diferença estatisticamente significativa da imunoexpressão do IFN- entre os epitélios (p=0,565) e cápsulas (p=0,414) dos cistos radiculares e cistos dentígeros. Além disso, não houve diferença estatisticamente significativa da imunoexpressão do TGF-β1 entre os epitélios (p=0,620) e cápsulas (p=0,056) dos cistos radiculares e cistos dentígeros. O teste de Wilcoxon revelou que não houve diferença estatisticamente significativa entre as imunoexpressões do IFN- e do TGF-β1 no epitélio (p=0,225) e na cápsula (p=0,370) dos cistos radiculares. Não houve diferença estatisticamente significativa entre as imunoexpressões do IFN- e do TGF-β1 no epitélio (p=0,361) dos cistos dentígeros. No entanto, houve diferença estatisticamente significativa entre as imunoexpressões do IFN- e do TGF-β1 na cápsula (p=0,001) dos cistos dentígeros, sendo o TGF-β1 o que apresentou a imunoexpressão mais significativa. Diante destes resultados, concluímos que não houve diferença de expressão imuno-histoquímica do IFN-γ e do TGF-β1 entre os cistos radiculares e dentígeros e que o TGF-β1 foi mais expressivo do que o IFN-γ na cápsula dos cistos dentígerosConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Patologia OralUFRNBROdontologiaCisto radicularCisto dentígeroImuno-histoquímicaInterferon gamaFatores de crescimento transformadoresRadicular cystDentigerous cystImmunohistochemistryInterferon gammaTransforming growth factorsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAExpressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígerosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALPedroCRN_DISSERT.pdfapplication/pdf3117630https://repositorio.ufrn.br/bitstream/123456789/17123/1/PedroCRN_DISSERT.pdf2544a3022fe67731388c2238d7dc5289MD51TEXTPedroCRN_DISSERT.pdf.txtPedroCRN_DISSERT.pdf.txtExtracted texttext/plain131868https://repositorio.ufrn.br/bitstream/123456789/17123/6/PedroCRN_DISSERT.pdf.txt6e7e9e9342465d1a06c047b1c6097833MD56THUMBNAILPedroCRN_DISSERT.pdf.jpgPedroCRN_DISSERT.pdf.jpgIM Thumbnailimage/jpeg3749https://repositorio.ufrn.br/bitstream/123456789/17123/7/PedroCRN_DISSERT.pdf.jpgafec12a356af839a2f836fc7d47b9b47MD57123456789/171232017-11-04 13:13:13.125oai:https://repositorio.ufrn.br:123456789/17123Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T16:13:13Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
title |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
spellingShingle |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros Rocha Neto, Pedro Carlos da Cisto radicular Cisto dentígero Imuno-histoquímica Interferon gama Fatores de crescimento transformadores Radicular cyst Dentigerous cyst Immunohistochemistry Interferon gamma Transforming growth factors CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
title_full |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
title_fullStr |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
title_full_unstemmed |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
title_sort |
Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros |
author |
Rocha Neto, Pedro Carlos da |
author_facet |
Rocha Neto, Pedro Carlos da |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5529085665771149 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786083Z7&dataRevisao=null |
dc.contributor.referees1.pt_BR.fl_str_mv |
Morais, Maria de Lourdes Silva Arruda |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/2371541508231170 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Galvão, Hébel Cavalcanti |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9 |
dc.contributor.author.fl_str_mv |
Rocha Neto, Pedro Carlos da |
dc.contributor.advisor1.fl_str_mv |
Costa, Antônio de Lisboa Lopes |
contributor_str_mv |
Costa, Antônio de Lisboa Lopes |
dc.subject.por.fl_str_mv |
Cisto radicular Cisto dentígero Imuno-histoquímica Interferon gama Fatores de crescimento transformadores |
topic |
Cisto radicular Cisto dentígero Imuno-histoquímica Interferon gama Fatores de crescimento transformadores Radicular cyst Dentigerous cyst Immunohistochemistry Interferon gamma Transforming growth factors CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Radicular cyst Dentigerous cyst Immunohistochemistry Interferon gamma Transforming growth factors |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-γ and TGF-β1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-γ and TGF-β1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-γ and TGF-β1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-γ between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-β1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-γ and TGF-β1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-γ and TGF-β1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-β1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-γ and TGF-β1 between radicular and dentigerous cysts and that TGF-β1 was more significant than the IFN-γ in the capsule of dentigerous cysts |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-11-22 2014-12-17T15:32:21Z |
dc.date.issued.fl_str_mv |
2012-02-29 |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:32:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROCHA NETO, Pedro Carlos da. Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros. 2012. 81 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/17123 |
identifier_str_mv |
ROCHA NETO, Pedro Carlos da. Expressão imuno-histoquímica das proteínas IFN-ƴ e TGF-β1 em cistos radiculares e cistos dentígeros. 2012. 81 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2012. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/17123 |
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por |
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Universidade Federal do Rio Grande do Norte |
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Programa de Pós-Graduação em Patologia Oral |
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UFRN |
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BR |
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Odontologia |
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Universidade Federal do Rio Grande do Norte |
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