Variabilidade genética de Melipona capixaba MOURE & CAMARGO, 1994 (Hymenoptera: Apidae), espécie ameaçada de extinção: subsídios para sua conservação

• Ano de defesa: 2009
• Autor(a) principal: Nogueira, Juliano
• Orientador(a): Tavares, Mara Garcia
• Banca de defesa: Santos, Jorge Abdala Dergam dos , Dias, Luiz Antonio dos Santos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Melipona capixaba; ISSR; Variabilidade
• Palavras-chave em Inglês: Melipona capixaba; ISSR; Variability
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2315

Melipona capixaba is a type of threatened of extinction bee, endemic of Espírito Santo State, more specifically restricted to forest formations, in mountainous areas, in moutainous areas, nearby the municipal district of Domingos Martins (Mountainous area). The present study had as purpose to esteem the genetic variability of colonies of M. capixaba. For this, samples of 95 colonies of the species were used, originated from eight municipal capixabas districts. The samples had their DNA extracted and submitted to the amplification and the product of PCR was examined by eletroforesis in agarose gel (1,5%). A group of ten primers of ISSR was used in the analyses what resulted in a total of 118 bands, from which 61,02% were polymorphics. Nei's genetic diversity (1973) and the index of Shannon for the species were evaluated in 0,19 and 0,29, respectively. The matrix of genetic distance presented low genetic distances among the colonies of the 23 selected places and there was not relationship among the geographical and genetic distances. The grouping analyses (UPGMA), as well as the three-dimensional projections showed the existence of genetic structuring, however this one doesn't have relationship with the geographical distances. AMOVA demonstrated that most of the variability is owed to the existent variations inside the places. Using the software STRUCTURE, the colonies were organized in four groups and 25 subdivisions, differing of the previous analyses, that made possible a better separation of the samples. This division in groups and subgrupos can serve as starting point for the handling strategies and conservation.