Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino
Ano de defesa: | 2015 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual Paulista (Unesp)
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Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
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País: |
Não Informado pela instituição
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Palavras-chave em Português: | |
Link de acesso: | http://hdl.handle.net/11449/140248 |
Resumo: | Cervical cancer is a worldwide public health problem, especially in developing countries, setting the fourth most common female cancer in the world. The infection by human papillomavirus (HPV) is the main risk factor for the development of this cancer, being present in 90% of cases of cervical cancer in the world population. Forward the search for new chemotherapeutic agents, natural products have strongly contributed to the identification of these substances and the development of modern therapeutics. Maytenin is a quinonemethide triterpene extracted from the roots of Maytenus ilicifolia plant, which has some biological activities previously reported as an antifungal, antiprotozoal and especially antitumor action. At the moment there are no reports in the literature relating the activity of maytenin in infected cells by HPV-16. The aim of this study was to evaluate the cytotoxicity of maytenin in cervical carcinoma line infected by HPV-16 (SiHa), non-infected by the virus (C-33 A) and by immortalized human keratinocytes (HaCaT), as well as verifying the induction of apoptosis and / or necrosis by Hoechst test and propidium iodide and the induction of apoptosis by caspase-3 activity in all three cell lines, in addition to check for changes in the cell cycle of treated cells with maytenin and the potential of genotoxicity and mutagenicity, and to evaluate the proliferation of the three cell lines. The cytotoxicity assay was performed by sulforhodamine B method and the three cell lines were treated during 6, 12 or 24 hours. It was possible to observe a concentration effect and response time in three lines analyzed. The IC50 was 3,26, 8,49 and 3,09 μM for SiHa, C-33 A and HaCaT lines respectively, in 24 hours treatment time. In order to evaluate the potential inductive effect of apoptosis it was performed a marking cytomorphological test with Hoechst and propidium iodide in 6 and 12 hours treatment times, and for the three cell lines it was ... |
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Estudo do potencial terapêutico da maitenina em células de câncer de colo uterinoColo uterino - CâncerEspinheira santaAgentes antineoplasicosVirus do papilomaCervix uteri CâncerCervical cancer is a worldwide public health problem, especially in developing countries, setting the fourth most common female cancer in the world. The infection by human papillomavirus (HPV) is the main risk factor for the development of this cancer, being present in 90% of cases of cervical cancer in the world population. Forward the search for new chemotherapeutic agents, natural products have strongly contributed to the identification of these substances and the development of modern therapeutics. Maytenin is a quinonemethide triterpene extracted from the roots of Maytenus ilicifolia plant, which has some biological activities previously reported as an antifungal, antiprotozoal and especially antitumor action. At the moment there are no reports in the literature relating the activity of maytenin in infected cells by HPV-16. The aim of this study was to evaluate the cytotoxicity of maytenin in cervical carcinoma line infected by HPV-16 (SiHa), non-infected by the virus (C-33 A) and by immortalized human keratinocytes (HaCaT), as well as verifying the induction of apoptosis and / or necrosis by Hoechst test and propidium iodide and the induction of apoptosis by caspase-3 activity in all three cell lines, in addition to check for changes in the cell cycle of treated cells with maytenin and the potential of genotoxicity and mutagenicity, and to evaluate the proliferation of the three cell lines. The cytotoxicity assay was performed by sulforhodamine B method and the three cell lines were treated during 6, 12 or 24 hours. It was possible to observe a concentration effect and response time in three lines analyzed. The IC50 was 3,26, 8,49 and 3,09 μM for SiHa, C-33 A and HaCaT lines respectively, in 24 hours treatment time. In order to evaluate the potential inductive effect of apoptosis it was performed a marking cytomorphological test with Hoechst and propidium iodide in 6 and 12 hours treatment times, and for the three cell lines it was ...O câncer de colo de útero é um problema de saúde pública mundial, especialmente nos países em desenvolvimento, configurando a quarta neoplasia feminina mais comum no mundo. A infecção pelo Papilomavírus Humano (HPV) é o principal fator de risco para o desenvolvimento do referido câncer, estando presente em 90% dos casos de câncer de colo uterino na população mundial. Frente a busca por novos agentes quimioterapêuticos, os produtos naturais tem contríbuido intensamente para a identificação dessas substâncias e o desenvolvimento da terapêutica moderna. Maitenina, é um triterpeno quinonametídeo extraído das raízes da plana Maytenus ilicifolia, que possui algumas atividades biológicas já relatadas, como antifúngica, antiprotozoária e principalmente ação antitumoral. No momento não existem relatos na literatura relacionando a atividade da maitenina em células infectadas por HPV-16. O objetivo do presente estudo foi avaliar a citotoxicidade da maitenina em linhagem de carcinoma cervical infectada por HPV-16 (SiHa), não-infectada pelo vírus (C-33 A) e queratinócitos humanos imortalizados (HaCaT), bem como verificar a indução de apoptose e/ou necrose pelo ensaio de Hoechst e Iodeto de Propídeo e indução de apoptose pela atividade de caspase-3 nas três linhagens celulares, além de verificar alterações no ciclo celular das células tratadas com maitenina e o potencial de genotoxicidade e mutagenicidade, além de avaliar a proliferação das três linhagens. O ensaio de citotoxicidade foi realizado pelo método da sulforodamina B, e as três linhagens celulares foram tratadas durante 6, 12 ou 24 horas. Foi possível observar um efeito concentração e tempo resposta nas três linhagens analisadas. O CI50 foi de 3,26, 8,49 e 3,09 μM para as linhagens SiHa, C-33 A e HaCaT respectivamente, no tempo de tratamento de 24 horas. Com o intuito de avaliar o potencial efeito indutor de apoptose foi realizado o ensaio...Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Soares, Christiane Pienna [UNESP]Valente, Valeria [UNESP]Universidade Estadual Paulista (Unesp)Galiardi, Ana Emília Brumatti [UNESP]2016-07-01T13:10:33Z2016-07-01T13:10:33Z2015-10-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis67 f : figs.application/pdfGALIARDI, Ana Emília Brumatti. Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino. 2015. 67 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2015.http://hdl.handle.net/11449/140248000858684000858684.pdf33004030081P7176802529037366950506531534378980000-0003-1740-7360Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-24T18:09:40Zoai:repositorio.unesp.br:11449/140248Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:08:29.576919Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
title |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
spellingShingle |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino Galiardi, Ana Emília Brumatti [UNESP] Colo uterino - Câncer Espinheira santa Agentes antineoplasicos Virus do papiloma Cervix uteri Câncer |
title_short |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
title_full |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
title_fullStr |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
title_full_unstemmed |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
title_sort |
Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino |
author |
Galiardi, Ana Emília Brumatti [UNESP] |
author_facet |
Galiardi, Ana Emília Brumatti [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Soares, Christiane Pienna [UNESP] Valente, Valeria [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Galiardi, Ana Emília Brumatti [UNESP] |
dc.subject.por.fl_str_mv |
Colo uterino - Câncer Espinheira santa Agentes antineoplasicos Virus do papiloma Cervix uteri Câncer |
topic |
Colo uterino - Câncer Espinheira santa Agentes antineoplasicos Virus do papiloma Cervix uteri Câncer |
description |
Cervical cancer is a worldwide public health problem, especially in developing countries, setting the fourth most common female cancer in the world. The infection by human papillomavirus (HPV) is the main risk factor for the development of this cancer, being present in 90% of cases of cervical cancer in the world population. Forward the search for new chemotherapeutic agents, natural products have strongly contributed to the identification of these substances and the development of modern therapeutics. Maytenin is a quinonemethide triterpene extracted from the roots of Maytenus ilicifolia plant, which has some biological activities previously reported as an antifungal, antiprotozoal and especially antitumor action. At the moment there are no reports in the literature relating the activity of maytenin in infected cells by HPV-16. The aim of this study was to evaluate the cytotoxicity of maytenin in cervical carcinoma line infected by HPV-16 (SiHa), non-infected by the virus (C-33 A) and by immortalized human keratinocytes (HaCaT), as well as verifying the induction of apoptosis and / or necrosis by Hoechst test and propidium iodide and the induction of apoptosis by caspase-3 activity in all three cell lines, in addition to check for changes in the cell cycle of treated cells with maytenin and the potential of genotoxicity and mutagenicity, and to evaluate the proliferation of the three cell lines. The cytotoxicity assay was performed by sulforhodamine B method and the three cell lines were treated during 6, 12 or 24 hours. It was possible to observe a concentration effect and response time in three lines analyzed. The IC50 was 3,26, 8,49 and 3,09 μM for SiHa, C-33 A and HaCaT lines respectively, in 24 hours treatment time. In order to evaluate the potential inductive effect of apoptosis it was performed a marking cytomorphological test with Hoechst and propidium iodide in 6 and 12 hours treatment times, and for the three cell lines it was ... |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-23 2016-07-01T13:10:33Z 2016-07-01T13:10:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GALIARDI, Ana Emília Brumatti. Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino. 2015. 67 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2015. http://hdl.handle.net/11449/140248 000858684 000858684.pdf 33004030081P7 1768025290373669 5050653153437898 0000-0003-1740-7360 |
identifier_str_mv |
GALIARDI, Ana Emília Brumatti. Estudo do potencial terapêutico da maitenina em células de câncer de colo uterino. 2015. 67 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2015. 000858684 000858684.pdf 33004030081P7 1768025290373669 5050653153437898 0000-0003-1740-7360 |
url |
http://hdl.handle.net/11449/140248 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
67 f : figs. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808130129194385408 |