Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Produção Agrícola
|
Departamento: |
Unidade Acadêmica de Garanhuns
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6144 |
Resumo: | During storage, grains, seeds and byproducts are subject to attack by pests, which cause qualitative and quantitative losses as reduces the nutritional value of the product and business. To avoid losses chemicals are used, many of which are showing failures in control, which has led to its widespread use. In search of new alternatives, research is being conducted with the goal of developing natural products. Given the diversity of substances extracted from lichens and knowledge about their biological activity, in this work the insecticidal potential of extracts of the lichen Cladonia substellata on beetles Callosobruchus maculatus and Zabrotes subfasciatus to evaluate the toxicity (repellent effect) and bioactivity on mortality, oviposition and emergence of insects in stored beans. For this, we used the meeting and ethereal extracts of C. substellata, which were obtained by successive extractions of exhaustion of the stem liquênico. The extracts were tested separately at rates of 0,02, 0,006 and 0,002 mg / mL on beans Vigna sp, to C. maculatus, and at doses of 0,02 and 0,002 mg / ml on Phaseolus sp beans to Z. subfasciatus. Analysis of thin layer chromatography (TLC) confirmed the presence of usnic acid as major compound. The extracts and ethereal meeting of C. substellata showed repellent effect and caused mortality with increasing dosages utilized in both insects. These results indicate that extracts of C. substellata act on the immature stages of C. maculatus and Z. subfasciatus, usnic acid being present in these extracts probably responsible for the effect ovicidal / larvicidal observed. Further studies with extracts of the lichen C. substellata should be conducted with other concentrations, to evaluate the selectivity and possible effects on the viability and germination of bean seeds. |
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OLIVEIRA, Carlos Romero Ferreira deSOUSA, Kátya Maria de OliveiraOLIVEIRA, Cláudia Helena Cysneiros Matos deBADJI, César Augustehttp://lattes.cnpq.br/7120383519899986MONTE, Denise Maria de Oliveira2016-12-14T17:23:49Z2012-12-17MONTE, Denise Maria de Oliveira. Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado. 2012. 67 f. Dissertação (Programa de Pós-Graduação em Produção Agrícola) - Universidade Federal Rural de Pernambuco, Garanhuns.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6144During storage, grains, seeds and byproducts are subject to attack by pests, which cause qualitative and quantitative losses as reduces the nutritional value of the product and business. To avoid losses chemicals are used, many of which are showing failures in control, which has led to its widespread use. In search of new alternatives, research is being conducted with the goal of developing natural products. Given the diversity of substances extracted from lichens and knowledge about their biological activity, in this work the insecticidal potential of extracts of the lichen Cladonia substellata on beetles Callosobruchus maculatus and Zabrotes subfasciatus to evaluate the toxicity (repellent effect) and bioactivity on mortality, oviposition and emergence of insects in stored beans. For this, we used the meeting and ethereal extracts of C. substellata, which were obtained by successive extractions of exhaustion of the stem liquênico. The extracts were tested separately at rates of 0,02, 0,006 and 0,002 mg / mL on beans Vigna sp, to C. maculatus, and at doses of 0,02 and 0,002 mg / ml on Phaseolus sp beans to Z. subfasciatus. Analysis of thin layer chromatography (TLC) confirmed the presence of usnic acid as major compound. The extracts and ethereal meeting of C. substellata showed repellent effect and caused mortality with increasing dosages utilized in both insects. These results indicate that extracts of C. substellata act on the immature stages of C. maculatus and Z. subfasciatus, usnic acid being present in these extracts probably responsible for the effect ovicidal / larvicidal observed. Further studies with extracts of the lichen C. substellata should be conducted with other concentrations, to evaluate the selectivity and possible effects on the viability and germination of bean seeds.Durante o armazenamento, os grãos, sementes e seus subprodutos estão sujeitos ao ataque de pragas, os quais ocasionam perdas qualitativas e quantitativas, como redução dos valores nutricionais e comerciais do produto. Para evitar perdas são utilizados produtos químicos, muitos dos quais vêm apresentando falhas no controle, o que tem levado ao seu uso indiscriminado. Em busca de novas alternativas, pesquisas vêm sendo realizadas com o objetivo de desenvolver produtos de origem natural. Tendo em vista a diversidade de substâncias extraídas de liquens e o conhecimento acerca de sua atividade biológica, no presente trabalho foi estudado o potencial inseticida de extratos obtidos do líquen Cladonia substellata sobre os coleópteros Callosobruchus maculatus e Zabrotes subfasciatus, avaliando a toxicidade (efeito repelente) e a bioatividade sobre a mortalidade, emergência e oviposição dos insetos em feijão armazenado. Para isso, foram utilizados os extratos reunido e etéreo de C. substellata, os quais foram obtidos através de extrações sucessivas por esgotamento do talo liquênico. Os extratos foram testados, separadamente, nas doses de 0,02, 0,006 e 0,002 mg/mL sobre os grãos de feijão Vigna sp, para C. maculatus, e nas doses de 0,02 e 0,002 mg/mL sobre os grãos de feijão Phaseolus sp, para Z. subfasciatus. A análise de cromatografia de camada delgada (CCD) confirmou a presença do ácido úsnico como composto majoritário. Os extratos reunido e etéreo de C. substellata mostraram efeito repelente e ocasionaram mortalidades com o aumento das dosagens utilizadas nos dois insetos. Estes resultados indicam que os extratos de C. substellata atuam sobre as fases imaturas de C. maculatus e de Z. subfasciatus, sendo o ácido úsnico presente nesses extratos, provavelmente, o responsável pelo efeito ovicida/larvicida observado. Novos estudos com os extratos do liquen C. substellata devem ser realizados com outras concentrações, para se avaliar a seletividade e possíveis efeitos sobre o poder germinativo e a viabilidade de sementes de feijão.Submitted by (edna.saturno@ufrpe.br) on 2016-12-14T17:23:49Z No. of bitstreams: 1 Denise Maria de Oliveira Monte.pdf: 637462 bytes, checksum: 88837ffe6b0dbe1dca832eda09cddd1f (MD5)Made available in DSpace on 2016-12-14T17:23:49Z (GMT). No. of bitstreams: 1 Denise Maria de Oliveira Monte.pdf: 637462 bytes, checksum: 88837ffe6b0dbe1dca832eda09cddd1f (MD5) Previous issue date: 2012-12-17Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Produção AgrícolaUFRPEBrasilUnidade Acadêmica de GaranhunsBioinseticidaCladonia substellataControle alternativoControle de pragaFeijãoCIENCIAS AGRARIASPotencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenadoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-723645219733019298560060060060077205141824112794117828424726906663919-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6144/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALDenise Maria de Oliveira Monte.pdfDenise Maria de Oliveira Monte.pdfapplication/pdf637462http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6144/2/Denise+Maria+de+Oliveira+Monte.pdf88837ffe6b0dbe1dca832eda09cddd1fMD52tede2/61442018-08-23 09:24:00.38oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:33:58.578215Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
title |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
spellingShingle |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado MONTE, Denise Maria de Oliveira Bioinseticida Cladonia substellata Controle alternativo Controle de praga Feijão CIENCIAS AGRARIAS |
title_short |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
title_full |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
title_fullStr |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
title_full_unstemmed |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
title_sort |
Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado |
author |
MONTE, Denise Maria de Oliveira |
author_facet |
MONTE, Denise Maria de Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
OLIVEIRA, Carlos Romero Ferreira de |
dc.contributor.referee1.fl_str_mv |
SOUSA, Kátya Maria de Oliveira |
dc.contributor.referee2.fl_str_mv |
OLIVEIRA, Cláudia Helena Cysneiros Matos de |
dc.contributor.referee3.fl_str_mv |
BADJI, César Auguste |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7120383519899986 |
dc.contributor.author.fl_str_mv |
MONTE, Denise Maria de Oliveira |
contributor_str_mv |
OLIVEIRA, Carlos Romero Ferreira de SOUSA, Kátya Maria de Oliveira OLIVEIRA, Cláudia Helena Cysneiros Matos de BADJI, César Auguste |
dc.subject.por.fl_str_mv |
Bioinseticida Cladonia substellata Controle alternativo Controle de praga |
topic |
Bioinseticida Cladonia substellata Controle alternativo Controle de praga Feijão CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Feijão |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
During storage, grains, seeds and byproducts are subject to attack by pests, which cause qualitative and quantitative losses as reduces the nutritional value of the product and business. To avoid losses chemicals are used, many of which are showing failures in control, which has led to its widespread use. In search of new alternatives, research is being conducted with the goal of developing natural products. Given the diversity of substances extracted from lichens and knowledge about their biological activity, in this work the insecticidal potential of extracts of the lichen Cladonia substellata on beetles Callosobruchus maculatus and Zabrotes subfasciatus to evaluate the toxicity (repellent effect) and bioactivity on mortality, oviposition and emergence of insects in stored beans. For this, we used the meeting and ethereal extracts of C. substellata, which were obtained by successive extractions of exhaustion of the stem liquênico. The extracts were tested separately at rates of 0,02, 0,006 and 0,002 mg / mL on beans Vigna sp, to C. maculatus, and at doses of 0,02 and 0,002 mg / ml on Phaseolus sp beans to Z. subfasciatus. Analysis of thin layer chromatography (TLC) confirmed the presence of usnic acid as major compound. The extracts and ethereal meeting of C. substellata showed repellent effect and caused mortality with increasing dosages utilized in both insects. These results indicate that extracts of C. substellata act on the immature stages of C. maculatus and Z. subfasciatus, usnic acid being present in these extracts probably responsible for the effect ovicidal / larvicidal observed. Further studies with extracts of the lichen C. substellata should be conducted with other concentrations, to evaluate the selectivity and possible effects on the viability and germination of bean seeds. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-12-17 |
dc.date.accessioned.fl_str_mv |
2016-12-14T17:23:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MONTE, Denise Maria de Oliveira. Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado. 2012. 67 f. Dissertação (Programa de Pós-Graduação em Produção Agrícola) - Universidade Federal Rural de Pernambuco, Garanhuns. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6144 |
identifier_str_mv |
MONTE, Denise Maria de Oliveira. Potencial bioinseticida de extratos de Cladonia substellata Vainio sobre pragas de feijão armazenado. 2012. 67 f. Dissertação (Programa de Pós-Graduação em Produção Agrícola) - Universidade Federal Rural de Pernambuco, Garanhuns. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6144 |
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