Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Moura, Jonas Marçal Lázaro de lattes
Orientador(a): Kinoshita, ângela Mitie Otta lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: IASCJ - Universidade Sagrado Coração
Programa de Pós-Graduação: Biologia Oral
Departamento: Ciências da Saúde e Biológicas
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://localhost:8080/tede/handle/tede/142
Resumo: Natural rubber membranes made of latex extracted from Hevea brasiliensis are capable to stimulate angiogenesis and accelerates the repair process. The latex is usually preserved in alkaline medium, consisting of ammonia solution, and one question that remains concerns the effect of this substance in the behavior of this biomaterial. Therefore, the objective of this comparative study was to assess the performance of membranes made of natural latex prepared with three different methods: polymerized immediately after collection without the use of ammonia (L1); polymerized after preservation in ammonia solution (L2); and polymerized after storage in ammonia, followed by Soxhlet technique for the extensive extraction of substances (L3). Polytetrafluoroethylene (PTFE) membrane, the gold standard in Guided tissue regeneration procedures was used as control. Thirty adult male New Zealand rabbits were used in the study. Two 10 mm diameter bone defects were surgically made in the calvaria of the animals. Defects were treated with GBR using L1, L2, L3 or PTFE membranes (n=15 for each membrane). Ten animals at a time were euthanized after 7, 20 and 60 days postoperatively. For each kind of membrane, a total of 5 histological samples were obtained, and bone regeneration results were assessed microscopically. Samples collected at 7 and 20 days after surgery presented no significant differences among treatments. After 60 days, defects covered with L1 membranes showed a significantly larger bone formation area, evaluated by histomorphometry, in comparison to the other groups (P<0.05, ANOVA, Tukey). In addition, bone tissue hypersensibilization for L1 and PTFE membranes was also investigated, in 6 additional rabbits with no differences being found, in the microscopic analysis, between samples collected from sensibilized and non-sensibilized animals. It can be concluded that: i) the natural rubber latex membranes were effective as a biological barrier ii) membranes prepared without ammonia presented better results to other membranes and the control iii) the latex membrane without ammonia is biocompatible, and do not caused sensitization of the bone tissue
id USC_9df34e9095370fe60ed694f6cea57872
oai_identifier_str oai:localhost:tede/142
network_acronym_str USC
network_name_str Biblioteca Digital de Teses e Dissertações da USC
repository_id_str
spelling Kinoshita, ângela Mitie OttaCPF:10915343819http://lattes.cnpq.br/9357035017384643CPF:65501934949http://lattes.cnpq.br/3570785236212803Moura, Jonas Marçal Lázaro de2015-09-14T13:12:51Z2012-09-292012-02-28MOURA, Jonas Marçal Lázaro de. Comparative study of the performance of membranes natural latex extracted from Hevea brasiliensis in different preparations on the repair of bone defects. 2012. 61 f. Tese (Doutorado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2012.http://localhost:8080/tede/handle/tede/142Natural rubber membranes made of latex extracted from Hevea brasiliensis are capable to stimulate angiogenesis and accelerates the repair process. The latex is usually preserved in alkaline medium, consisting of ammonia solution, and one question that remains concerns the effect of this substance in the behavior of this biomaterial. Therefore, the objective of this comparative study was to assess the performance of membranes made of natural latex prepared with three different methods: polymerized immediately after collection without the use of ammonia (L1); polymerized after preservation in ammonia solution (L2); and polymerized after storage in ammonia, followed by Soxhlet technique for the extensive extraction of substances (L3). Polytetrafluoroethylene (PTFE) membrane, the gold standard in Guided tissue regeneration procedures was used as control. Thirty adult male New Zealand rabbits were used in the study. Two 10 mm diameter bone defects were surgically made in the calvaria of the animals. Defects were treated with GBR using L1, L2, L3 or PTFE membranes (n=15 for each membrane). Ten animals at a time were euthanized after 7, 20 and 60 days postoperatively. For each kind of membrane, a total of 5 histological samples were obtained, and bone regeneration results were assessed microscopically. Samples collected at 7 and 20 days after surgery presented no significant differences among treatments. After 60 days, defects covered with L1 membranes showed a significantly larger bone formation area, evaluated by histomorphometry, in comparison to the other groups (P<0.05, ANOVA, Tukey). In addition, bone tissue hypersensibilization for L1 and PTFE membranes was also investigated, in 6 additional rabbits with no differences being found, in the microscopic analysis, between samples collected from sensibilized and non-sensibilized animals. It can be concluded that: i) the natural rubber latex membranes were effective as a biological barrier ii) membranes prepared without ammonia presented better results to other membranes and the control iii) the latex membrane without ammonia is biocompatible, and do not caused sensitization of the bone tissueAs membranas de látex natural, extraído da Hevea brasiliensis, estimula a angiogênes e acelera o processo de reparo. Porém, usualmente o látex é preservado em meio alcalino, constituído de solução de amônia e, uma questão que permanece diz respeito ao efeito dessa substância no comportamento deste biomaterial. Deste modo, esse trabalho consiste no estudo comparativo do desempenho de membranas de látex natural preparadas de 3 diferentes formas: polimerizadas sem amônia logo após a coleta (L1); polimerizadas após a sua conservação em solução de amônia (L2); e polimerizadas após conservação em amônia seguida por extensiva extração de substâncias por Soxhlet (L3). Membranas de politetrafluoretileno (PTFE), padrão ouro em procedimentos de ROG, foram usadas como controle. Para esta avaliação foram utilizados 30 coelhos machos adultos da raça Nova Zelândia. Dois defeitos ósseos de 10mm de diâmetro foram confeccionados cirurgicamente na calvária dos animais. Os defeitos foram tratados com ROG utilizando-se as membranas L1, L2, L3 ou PTFF (N=15 para cada grupo). Dez animais foram eutanasiados aos 7, 20 e aos 60 dias de pós-operatório. Para cada período, um total de 5 amostras foram obtidas para cada membrana e as peças avaliadas histologicamente. As amostras obtidas aos 7 e 20 dias não mostraram diferenças estatisticamente significantes entre os grupos. Aos 60 dias, os defeitos tratados com membranas L1 apresentaram uma formação óssea, avaliada por histomorfometria, significantemente maior em relação aos demais tratamentos (P<0.05, ANOVA, Tukey). Adicionalmente, a hipersensibilização do tecido ósseo por L1 e PTFE foi avaliada em outros 6 coelhos e nenhuma diferença foi notada, na análise microscópica, entre as peças coletadas com ou sem sensibilização prévia. Conclui-se que: i) as membranas de látex natural foram eficazes como barreira bilógica; ii) as membranas sem amônia evidenciaram comportamento muito superior às outras membranas teste e a membrana controle; iii) a membrana de látex sem amônia mostrou-se biocompatível, não causando sensibilização do tecido ósseo.Made available in DSpace on 2015-09-14T13:12:51Z (GMT). No. of bitstreams: 1 tese_jonas_marcal_lazaro_de_moura.pdf: 7013488 bytes, checksum: fd916ce3903bc744043c425a79a4be0f (MD5) Previous issue date: 2012-02-28application/pdfporIASCJ - Universidade Sagrado CoraçãoBiologia OralUSCBRCiências da Saúde e BiológicasREGENERAÇÃO ÓSSEALÁTEXAMÔNIAREGENERAÇÃO TECIDUAL GUIADAMEMBRANAS ARTIFICIAISTESTE DE MATERIAISLATEXBONE REGENERATIONAMMONIAGUIDED TISSUE REGENERATIONARTIFICIAL MEMBRANESMATERIALS TESTINGCIENCIAS DA SAUDE::ODONTOLOGIAEstudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseosComparative study of the performance of membranes natural latex extracted from Hevea brasiliensis in different preparations on the repair of bone defectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8043334104093724546600600600-753428887677182963-2070498469879244349info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da USCinstname:Universidade do Sagrado Coração (USC)instacron:USCORIGINALtese_jonas_marcal_lazaro_de_moura.pdfapplication/pdf7013488http://localhost:8080/tede/bitstream/tede/142/1/tese_jonas_marcal_lazaro_de_moura.pdffd916ce3903bc744043c425a79a4be0fMD51tede/1422015-10-16 14:43:18.904oai:localhost:tede/142Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.usc.br:8443/http://tede2.usc.br:8080/oai/requestbiblicorjesu@unisagrado.edu.br||normalizacao@usc.bropendoar:2015-10-16T17:43:18Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)false
dc.title.por.fl_str_mv Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
dc.title.alternative.eng.fl_str_mv Comparative study of the performance of membranes natural latex extracted from Hevea brasiliensis in different preparations on the repair of bone defects
title Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
spellingShingle Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
Moura, Jonas Marçal Lázaro de
REGENERAÇÃO ÓSSEA
LÁTEX
AMÔNIA
REGENERAÇÃO TECIDUAL GUIADA
MEMBRANAS ARTIFICIAIS
TESTE DE MATERIAIS
LATEX
BONE REGENERATION
AMMONIA
GUIDED TISSUE REGENERATION
ARTIFICIAL MEMBRANES
MATERIALS TESTING
CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
title_full Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
title_fullStr Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
title_full_unstemmed Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
title_sort Estudo comparativo do desempenho de membranas de látex natural extraído da hevea brasiliensis em diferentes preparos no reparo de defeitos ósseos
author Moura, Jonas Marçal Lázaro de
author_facet Moura, Jonas Marçal Lázaro de
author_role author
dc.contributor.advisor1.fl_str_mv Kinoshita, ângela Mitie Otta
dc.contributor.advisor1ID.fl_str_mv CPF:10915343819
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9357035017384643
dc.contributor.authorID.fl_str_mv CPF:65501934949
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3570785236212803
dc.contributor.author.fl_str_mv Moura, Jonas Marçal Lázaro de
contributor_str_mv Kinoshita, ângela Mitie Otta
dc.subject.por.fl_str_mv REGENERAÇÃO ÓSSEA
LÁTEX
AMÔNIA
REGENERAÇÃO TECIDUAL GUIADA
MEMBRANAS ARTIFICIAIS
TESTE DE MATERIAIS
topic REGENERAÇÃO ÓSSEA
LÁTEX
AMÔNIA
REGENERAÇÃO TECIDUAL GUIADA
MEMBRANAS ARTIFICIAIS
TESTE DE MATERIAIS
LATEX
BONE REGENERATION
AMMONIA
GUIDED TISSUE REGENERATION
ARTIFICIAL MEMBRANES
MATERIALS TESTING
CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv LATEX
BONE REGENERATION
AMMONIA
GUIDED TISSUE REGENERATION
ARTIFICIAL MEMBRANES
MATERIALS TESTING
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE::ODONTOLOGIA
description Natural rubber membranes made of latex extracted from Hevea brasiliensis are capable to stimulate angiogenesis and accelerates the repair process. The latex is usually preserved in alkaline medium, consisting of ammonia solution, and one question that remains concerns the effect of this substance in the behavior of this biomaterial. Therefore, the objective of this comparative study was to assess the performance of membranes made of natural latex prepared with three different methods: polymerized immediately after collection without the use of ammonia (L1); polymerized after preservation in ammonia solution (L2); and polymerized after storage in ammonia, followed by Soxhlet technique for the extensive extraction of substances (L3). Polytetrafluoroethylene (PTFE) membrane, the gold standard in Guided tissue regeneration procedures was used as control. Thirty adult male New Zealand rabbits were used in the study. Two 10 mm diameter bone defects were surgically made in the calvaria of the animals. Defects were treated with GBR using L1, L2, L3 or PTFE membranes (n=15 for each membrane). Ten animals at a time were euthanized after 7, 20 and 60 days postoperatively. For each kind of membrane, a total of 5 histological samples were obtained, and bone regeneration results were assessed microscopically. Samples collected at 7 and 20 days after surgery presented no significant differences among treatments. After 60 days, defects covered with L1 membranes showed a significantly larger bone formation area, evaluated by histomorphometry, in comparison to the other groups (P<0.05, ANOVA, Tukey). In addition, bone tissue hypersensibilization for L1 and PTFE membranes was also investigated, in 6 additional rabbits with no differences being found, in the microscopic analysis, between samples collected from sensibilized and non-sensibilized animals. It can be concluded that: i) the natural rubber latex membranes were effective as a biological barrier ii) membranes prepared without ammonia presented better results to other membranes and the control iii) the latex membrane without ammonia is biocompatible, and do not caused sensitization of the bone tissue
publishDate 2012
dc.date.available.fl_str_mv 2012-09-29
dc.date.issued.fl_str_mv 2012-02-28
dc.date.accessioned.fl_str_mv 2015-09-14T13:12:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOURA, Jonas Marçal Lázaro de. Comparative study of the performance of membranes natural latex extracted from Hevea brasiliensis in different preparations on the repair of bone defects. 2012. 61 f. Tese (Doutorado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2012.
dc.identifier.uri.fl_str_mv http://localhost:8080/tede/handle/tede/142
identifier_str_mv MOURA, Jonas Marçal Lázaro de. Comparative study of the performance of membranes natural latex extracted from Hevea brasiliensis in different preparations on the repair of bone defects. 2012. 61 f. Tese (Doutorado em Biologia Oral) - IASCJ - Universidade Sagrado Coração, Bauru, 2012.
url http://localhost:8080/tede/handle/tede/142
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 8043334104093724546
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv -753428887677182963
dc.relation.cnpq.fl_str_mv -2070498469879244349
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IASCJ - Universidade Sagrado Coração
dc.publisher.program.fl_str_mv Biologia Oral
dc.publisher.initials.fl_str_mv USC
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Ciências da Saúde e Biológicas
publisher.none.fl_str_mv IASCJ - Universidade Sagrado Coração
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da USC
instname:Universidade do Sagrado Coração (USC)
instacron:USC
instname_str Universidade do Sagrado Coração (USC)
instacron_str USC
institution USC
reponame_str Biblioteca Digital de Teses e Dissertações da USC
collection Biblioteca Digital de Teses e Dissertações da USC
bitstream.url.fl_str_mv http://localhost:8080/tede/bitstream/tede/142/1/tese_jonas_marcal_lazaro_de_moura.pdf
bitstream.checksum.fl_str_mv fd916ce3903bc744043c425a79a4be0f
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)
repository.mail.fl_str_mv biblicorjesu@unisagrado.edu.br||normalizacao@usc.br
_version_ 1815438185019211776