The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress

Detalhes bibliográficos
Ano de defesa: 2024
Autor(a) principal: Reis, André Guilherme Daubermann dos
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: eng
Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Solanum lycopersicum
Crescimento radicular
Drought stress
Estômatos
Estresse hídrico
Root growth
Stomata
Link de acesso: https://www.teses.usp.br/teses/disponiveis/11/11144/tde-12032025-105213/
Resumo: Plants face a plethora of environmental factors that cause various metabolic and physiological responses, with water deficit representing a significant abiotic stress that negatively affects growth and agricultural productivity. Several hormones have already been shown to participate in responses to abiotic stresses. Cultivated tomato plants are highly sensitive to drought stress. Investigating the relationship between one of the stress hormones (ethylene) and AtRALF1 in Chapter I, I confirm that ethylene is necessary for the AtRALF1-induced root growth inhibition and, for the first time, demonstrate that the hormone auxin is necessary for the AtRALF1-induced ethylene signalling. In Chapter II, in silico analyses were used to identify AtRALF1 and AtRALF4 homologs in the tomato genome. Thirteen AtRALF1 and AtRALF4 homolog sequences were identified in Solanum lycopersicum. The sequences of the tomato RALFs (SlRALFs) aid in formulating new hypotheses about their diversity and functions in plants. Lastly, in Chapter III, a physiological characterization of slralf1 and slralf2 mutants was performed, showing that SlRALF1 participates in the control of stomatal pore aperture. Together, the results described here expand our understanding of the hormones that govern stomatal pore aperture and demonstrate the potential use of RALFs as targets for improving drought stress tolerance in cultivated tomato plants, contributing to enhanced food security in environments with limited water resources.
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spelling The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stressA função do SlRALF1/2 na fisiologia do tomateiro e o crosstalk entre AtRALF1-etilenoauxina: o papel dos peptídeos RALF no estresse hídricoSolanum lycopersicumSolanum lycopersicumCrescimento radicularDrought stressEstômatosEstresse hídricoRoot growthStomataPlants face a plethora of environmental factors that cause various metabolic and physiological responses, with water deficit representing a significant abiotic stress that negatively affects growth and agricultural productivity. Several hormones have already been shown to participate in responses to abiotic stresses. Cultivated tomato plants are highly sensitive to drought stress. Investigating the relationship between one of the stress hormones (ethylene) and AtRALF1 in Chapter I, I confirm that ethylene is necessary for the AtRALF1-induced root growth inhibition and, for the first time, demonstrate that the hormone auxin is necessary for the AtRALF1-induced ethylene signalling. In Chapter II, in silico analyses were used to identify AtRALF1 and AtRALF4 homologs in the tomato genome. Thirteen AtRALF1 and AtRALF4 homolog sequences were identified in Solanum lycopersicum. The sequences of the tomato RALFs (SlRALFs) aid in formulating new hypotheses about their diversity and functions in plants. Lastly, in Chapter III, a physiological characterization of slralf1 and slralf2 mutants was performed, showing that SlRALF1 participates in the control of stomatal pore aperture. Together, the results described here expand our understanding of the hormones that govern stomatal pore aperture and demonstrate the potential use of RALFs as targets for improving drought stress tolerance in cultivated tomato plants, contributing to enhanced food security in environments with limited water resources.As plantas enfrentam uma série de fatores ambientais que provocam várias respostas metabólicas e fisiológicas, com o déficit hídrico representando um estresse abiótico significativo que afeta negativamente o crescimento e a produtividade das plantas cultivadas. Vários hormônios já tiveram sua participação demonstrada nas respostas a estresses abióticos. Plantas de tomate cultivadas são altamente sensíveis ao estresse hídrico. Investigando a relação entre um dos hormônios de estresse (Etileno) e AtRALF1 no Capítulo I, confirmo que etileno é necessário para a inibição do crescimento de raiz por AtRALF1, e pela primeira vez demonstro que o hormônio auxina é necessário para a sinalização de etileno induzida por AtRALF1. No Capítulo II, análises in silico são usadas para identificar homólogos ao AtRALF1 e AtRALF4 no genoma do tomateiro. Ao todo, treze sequências homólogas aos RALFs de Arabidopsis são identificadas em Solanum lycopersicum. As sequências dos peptídeos RALF de tomateiro (SlRALFs) auxiliam a formulas novas hipóteses sobre sua diversidade e função em plantas. Por fim, no capítulo III, foi feita a caracterização fisiológica dos mutantes slralf1 e slralf2, demonstrando o papel do SlRALF1 no controle da abertura do poro estomático. Em conjunto, os resultados aqui descritos ampliam nossa compreensão das interações hormonais que governam as respostas estomáticas ao déficit hídrico e mostram o potencial dos peptídeos RALF como alvos para melhorar a tolerância à seca em cultivos de tomateiro, contribuindo assim para a segurança alimentar em ambientes com recursos hídricos limitados.Biblioteca Digitais de Teses e Dissertações da USPMoura, Daniel Scherer deReis, André Guilherme Daubermann dos2024-12-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/11/11144/tde-12032025-105213/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2025-03-18T14:44:42Zoai:teses.usp.br:tde-12032025-105213Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212025-03-18T14:44:42Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
A função do SlRALF1/2 na fisiologia do tomateiro e o crosstalk entre AtRALF1-etilenoauxina: o papel dos peptídeos RALF no estresse hídrico
title The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
spellingShingle The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
Reis, André Guilherme Daubermann dos
Solanum lycopersicum
Solanum lycopersicum
Crescimento radicular
Drought stress
Estômatos
Estresse hídrico
Root growth
Stomata
title_short The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
title_full The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
title_fullStr The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
title_full_unstemmed The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
title_sort The function of SlRALF1/2 in tomato plant physiology and the crosstalk between AtRALF1-ethylene-auxin: the role of RALF peptides in water stress
author Reis, André Guilherme Daubermann dos
author_facet Reis, André Guilherme Daubermann dos
author_role author
dc.contributor.none.fl_str_mv Moura, Daniel Scherer de
dc.contributor.author.fl_str_mv Reis, André Guilherme Daubermann dos
dc.subject.por.fl_str_mv Solanum lycopersicum
Solanum lycopersicum
Crescimento radicular
Drought stress
Estômatos
Estresse hídrico
Root growth
Stomata
topic Solanum lycopersicum
Solanum lycopersicum
Crescimento radicular
Drought stress
Estômatos
Estresse hídrico
Root growth
Stomata
description Plants face a plethora of environmental factors that cause various metabolic and physiological responses, with water deficit representing a significant abiotic stress that negatively affects growth and agricultural productivity. Several hormones have already been shown to participate in responses to abiotic stresses. Cultivated tomato plants are highly sensitive to drought stress. Investigating the relationship between one of the stress hormones (ethylene) and AtRALF1 in Chapter I, I confirm that ethylene is necessary for the AtRALF1-induced root growth inhibition and, for the first time, demonstrate that the hormone auxin is necessary for the AtRALF1-induced ethylene signalling. In Chapter II, in silico analyses were used to identify AtRALF1 and AtRALF4 homologs in the tomato genome. Thirteen AtRALF1 and AtRALF4 homolog sequences were identified in Solanum lycopersicum. The sequences of the tomato RALFs (SlRALFs) aid in formulating new hypotheses about their diversity and functions in plants. Lastly, in Chapter III, a physiological characterization of slralf1 and slralf2 mutants was performed, showing that SlRALF1 participates in the control of stomatal pore aperture. Together, the results described here expand our understanding of the hormones that govern stomatal pore aperture and demonstrate the potential use of RALFs as targets for improving drought stress tolerance in cultivated tomato plants, contributing to enhanced food security in environments with limited water resources.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-11
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/11/11144/tde-12032025-105213/
url https://www.teses.usp.br/teses/disponiveis/11/11144/tde-12032025-105213/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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