Frutos liofilizados de Leandra australis e atividade em modelo animal de obesidade

• Ano de defesa: 2018
• Autor(a) principal: Nunes, Aline Martins
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Fruto brasileiro; Berrie; Antocianina; Polifenóis; Obesidade
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/3114

Introduction: This dissertation presents the studies developed from the Leandra australis plant among the Brazilian biodiversity, which has chemical potential and no studies were conducted for the purpose of technological prospection of this species. Objective: To characterize the botanical and chemical characteristics of L. australis fruits and evaluate the effect of the preventive administration of these on body weight, inflammatory parameters and oxidative stress in an obesity animal model. Materials and Methods: Leandra australis was collected and characterized by UNISUL botanist. The fruits were lyophilized and characterized chemically in terms of total extractable substances, CCD chromatographic profile, total polyphenols, total anthocyanins, total ash and acid insoluble and reducing sugar content (TAR). FLLA were administered in mice submitted to an animal model of obesity. The effects on body weight, body structure, lipid and protein damage and antioxidant defense in an obesity animal model were evaluated. Results: A L. selustris exsicata identified and confirmed by UNISUL botanist was deposited in the Laelia purpurata herbarium under the number SRS 773. The amount of STE in ethanol, water and 80% acidified ethanol was 43.3, 47.3% and 34.4% respectively. In the chromatographic profile, anthocyanin CYN-3-GLU and 2 unidentified anthocyanins, rutin and gallic acid were identified. FLLA had 44.2 mg / g PT; 3.12 mg / g AT; 3.3% of total ash; 0.4% ash insoluble in hydrochloric acid and 33% TAR. In an animal model of obesity, an increase in body weight, mesenteric fat, liver and spleen was observed in the obese control group. Daily consumption of FLLA provided normal maintenance of the liver and spleen weight of obese animals. The animals submitted to a hyperlipid diet presented increased lipid and protein damage in both mesenteric and liver fat. FLLA consumption provided these damages in obese animals. Conclusion: FLLAs have similar amounts of polyphenols and anthocyanins as other berries. In an animal model of obesity the constant administration of FLLA was able to protect the liver from possible steatosis, as well as reduced oxidative damage generated by obesity.