Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma
Ano de defesa: | 2016 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde
|
Departamento: |
Saúde
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/2863 |
Resumo: | Asthma is a chronic inflammatory lung disease characterized by bronchoconstriction, elevated number of inflammatory cells in the airways such as eosinophils, neutrophils, lymphocytes, mast cells among others; increased mucus production and hyperresponsiveness. Considering that asthma may present more severe and resistant characteristics to conventional treatments, new therapies are of great importance. Phototherapy as Light-emitting diode (LED) has emerged as a treatment that presents good results for diseases which are characterized by inflammation. Thus, our objective was to investigate the effects of LED on the development of allergic lung inflammation. Male Balb/c mice were or not sensitized and challenged with ovalbumin (OVA) and treated or not with an LED therapy (1h and 4 h after each OVA challenge). Twenty-four hours after the last OVA challenge, analyzes were performed. We prioritized to analyze the total and differential cells present in bronchoalveolar lavage (BAL), blood and bone marrow, determination of mucus production, oedema, mast cell degranulation, and tracheal responsiveness. In addition, we investigated the levels of inflammatory and anti-inflammatory cytokines. Our results showed that LED treatment in asthmatic mice reduced the lung cell infiltration to the lung where can be observed by reduced number of both, total and differential cells (macrophages, lymphocytes and granulocytes). We also noted reduced number of eosinophils and neutrophils in the blood after LED treatment, and reestablishment of total cells found in the bone marrow. LED treatment did not alter the levels of inflammatory cytokines, but increased the levels of anti-inflammatory cytokines such as IL-10 and IFN-gamma. Moreover, LED treatment reduced mast cells degranulation, mucus production, oedema, and the tracheal contractile response. In conclusion, LED therapy reduced several important inflammatory parameters involved with worsening of asthma. Thus, this study may provide important information about the mechanisms involved in the LED effects on the respiratory tract, and in addition, it may open the possibility to a new approach for the treatment of asthma. |
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Franco, Adriana Lino dos SantosFranco, Adriana Lino dos SantosZamuner, Stella ReginaBreithaupt, Ana Cristinahttp://lattes.cnpq.br/3631675661828499Siqueira, Vanessa Perosino Cardoso2022-04-11T19:22:31Z2016-12-07Siqueira, Vanessa Perosino Cardoso. Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma. 2016. 43 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2863Asthma is a chronic inflammatory lung disease characterized by bronchoconstriction, elevated number of inflammatory cells in the airways such as eosinophils, neutrophils, lymphocytes, mast cells among others; increased mucus production and hyperresponsiveness. Considering that asthma may present more severe and resistant characteristics to conventional treatments, new therapies are of great importance. Phototherapy as Light-emitting diode (LED) has emerged as a treatment that presents good results for diseases which are characterized by inflammation. Thus, our objective was to investigate the effects of LED on the development of allergic lung inflammation. Male Balb/c mice were or not sensitized and challenged with ovalbumin (OVA) and treated or not with an LED therapy (1h and 4 h after each OVA challenge). Twenty-four hours after the last OVA challenge, analyzes were performed. We prioritized to analyze the total and differential cells present in bronchoalveolar lavage (BAL), blood and bone marrow, determination of mucus production, oedema, mast cell degranulation, and tracheal responsiveness. In addition, we investigated the levels of inflammatory and anti-inflammatory cytokines. Our results showed that LED treatment in asthmatic mice reduced the lung cell infiltration to the lung where can be observed by reduced number of both, total and differential cells (macrophages, lymphocytes and granulocytes). We also noted reduced number of eosinophils and neutrophils in the blood after LED treatment, and reestablishment of total cells found in the bone marrow. LED treatment did not alter the levels of inflammatory cytokines, but increased the levels of anti-inflammatory cytokines such as IL-10 and IFN-gamma. Moreover, LED treatment reduced mast cells degranulation, mucus production, oedema, and the tracheal contractile response. In conclusion, LED therapy reduced several important inflammatory parameters involved with worsening of asthma. Thus, this study may provide important information about the mechanisms involved in the LED effects on the respiratory tract, and in addition, it may open the possibility to a new approach for the treatment of asthma.A asma é uma doença inflamatória pulmonar crônica caracterizada por broncoconstrição, aumento do número de células inflamatórias no espaço aéreo (eosinófilos, mastócitos, neutrófilos, linfócitos entre outras), perda do epitélio brônquico, aumento na secreção de muco e reatividade exacerbada do músculo liso. Considerando que a asma pode apresentar formas mais graves e resistentes aos tratamentos convencionais, novas terapias são de grande importância. Nesse sentido, as fototerapias, como a de Diodo emissor de luz (LED), tem emergido como tratamento promissor, de baixo custo e isenta de efeitos colaterais, para diversas doenças com caráter inflamatório. Deste modo, nosso objetivo foi investigar os efeitos do tratamento com LED sobre o desencadeamento de inflamação pulmonar de origem alérgica. Camundongos machos Balb/c foram sensibilizados e desafiados com Ovoalbumina (OVA) e tratados ou não com LED (1 e 4 h após cada desafio com OVA). Após 24 horas do ultimo desafio com OVA as análises foram realizadas. Nós priorizamos a contagem do número de células totais e diferenciais no lavado broncoalveolar (LBA), sangue e medula óssea, determinação da produção de muco, edema, desgranulação de mastócitos e reatividade traqueal. Ainda, investigamos a liberação de citocinas inflamatórias e anti-inflamatórias. Nossos resultados mostraram que o tratamento com LED reduziu a infiltração de células para o pulmão observado tanto pelo número de células totais quanto diferenciais (macrófagos, linfócitos e granulócitos). Notamos ainda redução no número de eosinófilos e neutrófilos no sangue periférico após tratamento com LED e recuperação no número de células totais na medula óssea. O tratamento com LED não alterou a liberação de citocinas inflamatórias, mas aumentou os níveis de anti-inflamatórias como IL-10 e IFN-γ. Além disso, tratamento com LED reduziu a desgranulação de mastócitos, produção de muco, edema e resposta contrátil da traquéia mediante estímulo colinérgico. Em conclusão, o tratamento com LED reduziu diversos parâmetros inflamatórios importantes envolvidos com a piora da asma. Este estudo pode fornecer informações importantes sobre os mecanismos do LED no aparelho respiratório e, além disso, abre possibilidade de uma nova abordagem para o tratamento da asma.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-11T19:22:31Z No. of bitstreams: 1 Vanessa Perosino Cardoso Siqueira.pdf: 902444 bytes, checksum: 7de5f6d9d5f0d368510597299c733077 (MD5)Made available in DSpace on 2022-04-11T19:22:31Z (GMT). No. of bitstreams: 1 Vanessa Perosino Cardoso Siqueira.pdf: 902444 bytes, checksum: 7de5f6d9d5f0d368510597299c733077 (MD5) Previous issue date: 2016-12-07application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdeasmaLED terapiacitocinasprodução de mucoedemahiperreatividade das vias aéreasasthmaLED therapycytokinesmucus productionoedemaairways hyperresponsivenessCIENCIAS DA SAUDEEfeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asmaEffect of LED therapy on inflammatory parameters in an experimental model of asthmainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALVanessa Perosino Cardoso Siqueira.pdfVanessa Perosino Cardoso Siqueira.pdfapplication/pdf902444http://localhost:8080/tede/bitstream/tede/2863/2/Vanessa+Perosino+Cardoso+Siqueira.pdf7de5f6d9d5f0d368510597299c733077MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2863/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/28632022-04-11 16:22:31.801oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-04-11T19:22:31Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
dc.title.alternative.eng.fl_str_mv |
Effect of LED therapy on inflammatory parameters in an experimental model of asthma |
title |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
spellingShingle |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma Siqueira, Vanessa Perosino Cardoso asma LED terapia citocinas produção de muco edema hiperreatividade das vias aéreas asthma LED therapy cytokines mucus production oedema airways hyperresponsiveness CIENCIAS DA SAUDE |
title_short |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
title_full |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
title_fullStr |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
title_full_unstemmed |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
title_sort |
Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma |
author |
Siqueira, Vanessa Perosino Cardoso |
author_facet |
Siqueira, Vanessa Perosino Cardoso |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.referee1.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.referee2.fl_str_mv |
Zamuner, Stella Regina |
dc.contributor.referee3.fl_str_mv |
Breithaupt, Ana Cristina |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3631675661828499 |
dc.contributor.author.fl_str_mv |
Siqueira, Vanessa Perosino Cardoso |
contributor_str_mv |
Franco, Adriana Lino dos Santos Franco, Adriana Lino dos Santos Zamuner, Stella Regina Breithaupt, Ana Cristina |
dc.subject.por.fl_str_mv |
asma LED terapia citocinas produção de muco edema hiperreatividade das vias aéreas |
topic |
asma LED terapia citocinas produção de muco edema hiperreatividade das vias aéreas asthma LED therapy cytokines mucus production oedema airways hyperresponsiveness CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
asthma LED therapy cytokines mucus production oedema airways hyperresponsiveness |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Asthma is a chronic inflammatory lung disease characterized by bronchoconstriction, elevated number of inflammatory cells in the airways such as eosinophils, neutrophils, lymphocytes, mast cells among others; increased mucus production and hyperresponsiveness. Considering that asthma may present more severe and resistant characteristics to conventional treatments, new therapies are of great importance. Phototherapy as Light-emitting diode (LED) has emerged as a treatment that presents good results for diseases which are characterized by inflammation. Thus, our objective was to investigate the effects of LED on the development of allergic lung inflammation. Male Balb/c mice were or not sensitized and challenged with ovalbumin (OVA) and treated or not with an LED therapy (1h and 4 h after each OVA challenge). Twenty-four hours after the last OVA challenge, analyzes were performed. We prioritized to analyze the total and differential cells present in bronchoalveolar lavage (BAL), blood and bone marrow, determination of mucus production, oedema, mast cell degranulation, and tracheal responsiveness. In addition, we investigated the levels of inflammatory and anti-inflammatory cytokines. Our results showed that LED treatment in asthmatic mice reduced the lung cell infiltration to the lung where can be observed by reduced number of both, total and differential cells (macrophages, lymphocytes and granulocytes). We also noted reduced number of eosinophils and neutrophils in the blood after LED treatment, and reestablishment of total cells found in the bone marrow. LED treatment did not alter the levels of inflammatory cytokines, but increased the levels of anti-inflammatory cytokines such as IL-10 and IFN-gamma. Moreover, LED treatment reduced mast cells degranulation, mucus production, oedema, and the tracheal contractile response. In conclusion, LED therapy reduced several important inflammatory parameters involved with worsening of asthma. Thus, this study may provide important information about the mechanisms involved in the LED effects on the respiratory tract, and in addition, it may open the possibility to a new approach for the treatment of asthma. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-07 |
dc.date.accessioned.fl_str_mv |
2022-04-11T19:22:31Z |
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info:eu-repo/semantics/publishedVersion |
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dc.identifier.citation.fl_str_mv |
Siqueira, Vanessa Perosino Cardoso. Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma. 2016. 43 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2863 |
identifier_str_mv |
Siqueira, Vanessa Perosino Cardoso. Efeito da LED terapia sobre parâmetros inflamatórios em modelo experimental de asma. 2016. 43 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
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por |
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