A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal
Ano de defesa: | 2014 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de
|
Departamento: |
Sa??de
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/1312 |
Resumo: | Periodontitis is an infectious disease characterized by the destruction of the supporting tissues of the teeth and therefore the dental loss. Neutrophils are the first cells to arrive in periodontal inflammation and have as function phagocytosis and cytokine release. After a few days, the neutrophils are replaced by lymphocytes, which give the chronic nature of periodontitis. The established treatment for periodontitis is oral hygiene care, scaling and root planing, and in more advanced cases, there may be the need for systemic antibiotics. In order to reduce the prescription of antibiotics, it has been researched the use of photodynamic therapy (PDT) as antimicrobial intervention, with great results. The objective of this work is to verify if the PDT interferes with the chemotaxis of neutrophils and T lymphocytes into the periodontal tissue. For such, 05 patients who had undergone prior treatment with periodontal good oral hygiene and periodontal pockets with residual surgical indication were allocated. PDT was applied under the following parameters: methylene blue (50 ??g/ml) was applied to the bottom of the periodontal pocket and after 5 minutes, via transmucosal, was irradiated with diode laser 660 nm, P = 100 mW, t = 90 s per point 9 J/point, energy density: 22 J/cm2, power density: 250 mW/cm2. One week after PDT, the surgical removal of the periodontal pockets was performed and the samples sent for routine histology and immunohistochemistry for detection of neutrophil and lymphocyte. The samples were photographed and positive cells counted with the aid of ImageJ software. The data were evaluated statistically and no significant difference between groups (Mann-Whitney p = 0.5). It was concluded that PDT did not interfere with the chemotaxis of neutrophils and T lymphocytes in the periodontal tissue after 7 days. |
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Fran??a, Cristiane Mirandahttp://lattes.cnpq.br/3818545357716126Prates, Renato Araujohttp://lattes.cnpq.br/7664790931310514Fran??a, Cristiane Mirandahttp://lattes.cnpq.br/3818545357716126Fernandes, Adjaci Uch??ahttp://lattes.cnpq.br/9073409107055774Oliveira, Marcelo Tavares dehttp://lattes.cnpq.br/0262402232086674http://lattes.cnpq.br/5760354691330138Mernick, Ana Paula de Souza2016-05-19T15:00:56Z2014-12-16Mernick, Ana Paula de Souza. A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal. 2014. 52 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1312Periodontitis is an infectious disease characterized by the destruction of the supporting tissues of the teeth and therefore the dental loss. Neutrophils are the first cells to arrive in periodontal inflammation and have as function phagocytosis and cytokine release. After a few days, the neutrophils are replaced by lymphocytes, which give the chronic nature of periodontitis. The established treatment for periodontitis is oral hygiene care, scaling and root planing, and in more advanced cases, there may be the need for systemic antibiotics. In order to reduce the prescription of antibiotics, it has been researched the use of photodynamic therapy (PDT) as antimicrobial intervention, with great results. The objective of this work is to verify if the PDT interferes with the chemotaxis of neutrophils and T lymphocytes into the periodontal tissue. For such, 05 patients who had undergone prior treatment with periodontal good oral hygiene and periodontal pockets with residual surgical indication were allocated. PDT was applied under the following parameters: methylene blue (50 ??g/ml) was applied to the bottom of the periodontal pocket and after 5 minutes, via transmucosal, was irradiated with diode laser 660 nm, P = 100 mW, t = 90 s per point 9 J/point, energy density: 22 J/cm2, power density: 250 mW/cm2. One week after PDT, the surgical removal of the periodontal pockets was performed and the samples sent for routine histology and immunohistochemistry for detection of neutrophil and lymphocyte. The samples were photographed and positive cells counted with the aid of ImageJ software. The data were evaluated statistically and no significant difference between groups (Mann-Whitney p = 0.5). It was concluded that PDT did not interfere with the chemotaxis of neutrophils and T lymphocytes in the periodontal tissue after 7 days.A periodontite ?? uma doen??a infecciosa caracterizada pela destrui????o dos tecidos de suporte dos dentes e consequentemente ?? perda do elemento dental. Os neutr??filos s??o as primeiras c??lulas a chegar ao processo inflamat??rio periodontal e t??m como fun????o fagocitose e libera????o de citocinas. Ap??s alguns dias os neutr??filos v??o sendo gradualmente substitu??dos por linf??citos, que d??o o car??ter cr??nico da periodontite. O tratamento estabelecido para a periodontite ?? a orienta????o de higiene oral, raspagem e alisamento radicular e em casos mais avan??ados, pode haver a necessidade de antibioticoterapia sist??mica. No sentido de reduzir a prescri????o de antibi??ticos, tem-se pesquisando o uso da terapia fotodin??mica (PDT) como interven????o antimicrobiana, com ??timos resultados. Assim, o objetivo deste trabalho ?? verificar se a PDT interfere na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal. Para tal, foram alocados 05 pacientes que passaram por tratamento periodontal pr??vio com boa higiene oral e presen??a de bolsas periodontais residuais com indica????o cir??rgica. A PDT foi aplicada nos seguintes par??metros: azul de metileno (50 ??g/mL) aplicado no fundo da bolsa periodontal e ap??s 5 minutos foi irradiado via transmucosa com laser de diodo, 660 nm, P= 100 mW, t= 90 s por ponto, 9 J/ponto, densidade de energia: 22 J/cm2, densidade de pot??ncia: 250 mW/cm2. Uma semana ap??s a PDT foi realizada remo????o cir??rgica das bolsas e as amostras encaminhadas para processamento histol??gico de rotina e imunohistoqu??mica para detec????o de neutr??filos e linf??citos T. As amostras foram fotografadas e as c??lulas positivas contadas com aux??lio do software ImageJ. Os dados foram avaliados estatisticamente e n??o houve diferen??a significativa entre os grupos (Mann-Whitney p= 0,5). Conclui-se que a PDT n??o interfere na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal ap??s 7 dias.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2016-05-19T15:00:56Z No. of bitstreams: 1 Ana Paula de Souza Mernick.pdf: 747300 bytes, checksum: 7cec9bdaa4c2bd9e6dfcc62d9bfdbd12 (MD5)Made available in DSpace on 2016-05-19T15:00:56Z (GMT). No. of bitstreams: 1 Ana Paula de Souza Mernick.pdf: 747300 bytes, checksum: 7cec9bdaa4c2bd9e6dfcc62d9bfdbd12 (MD5) Previous issue date: 2014-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??deUNINOVEBrasilSa??deperiodontiteterapia fotodin??micaneutr??filoslinf??citos Tcitocinasperiodontitisphotodynamic therapyneutrophilsT lymphocytescytokinesCIENCIAS DA SAUDEA terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontalThe photodynamic therapy in neutrophil chemotaxis and T lymphocyte in periodontal tissueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALAna Paula de Souza Mernick.pdfAna Paula de Souza Mernick.pdfapplication/pdf747300http://localhost:8080/tede/bitstream/tede/1312/2/Ana+Paula+de+Souza+Mernick.pdf7cec9bdaa4c2bd9e6dfcc62d9bfdbd12MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1312/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/13122016-05-19 12:00:56.261oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2016-05-19T15:00:56Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
dc.title.alternative.eng.fl_str_mv |
The photodynamic therapy in neutrophil chemotaxis and T lymphocyte in periodontal tissue |
title |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
spellingShingle |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal Mernick, Ana Paula de Souza periodontite terapia fotodin??mica neutr??filos linf??citos T citocinas periodontitis photodynamic therapy neutrophils T lymphocytes cytokines CIENCIAS DA SAUDE |
title_short |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
title_full |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
title_fullStr |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
title_full_unstemmed |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
title_sort |
A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal |
author |
Mernick, Ana Paula de Souza |
author_facet |
Mernick, Ana Paula de Souza |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fran??a, Cristiane Miranda |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3818545357716126 |
dc.contributor.advisor-co1.fl_str_mv |
Prates, Renato Araujo |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7664790931310514 |
dc.contributor.referee1.fl_str_mv |
Fran??a, Cristiane Miranda |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3818545357716126 |
dc.contributor.referee2.fl_str_mv |
Fernandes, Adjaci Uch??a |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9073409107055774 |
dc.contributor.referee3.fl_str_mv |
Oliveira, Marcelo Tavares de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/0262402232086674 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5760354691330138 |
dc.contributor.author.fl_str_mv |
Mernick, Ana Paula de Souza |
contributor_str_mv |
Fran??a, Cristiane Miranda Prates, Renato Araujo Fran??a, Cristiane Miranda Fernandes, Adjaci Uch??a Oliveira, Marcelo Tavares de |
dc.subject.por.fl_str_mv |
periodontite terapia fotodin??mica neutr??filos linf??citos T citocinas |
topic |
periodontite terapia fotodin??mica neutr??filos linf??citos T citocinas periodontitis photodynamic therapy neutrophils T lymphocytes cytokines CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
periodontitis photodynamic therapy neutrophils T lymphocytes cytokines |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Periodontitis is an infectious disease characterized by the destruction of the supporting tissues of the teeth and therefore the dental loss. Neutrophils are the first cells to arrive in periodontal inflammation and have as function phagocytosis and cytokine release. After a few days, the neutrophils are replaced by lymphocytes, which give the chronic nature of periodontitis. The established treatment for periodontitis is oral hygiene care, scaling and root planing, and in more advanced cases, there may be the need for systemic antibiotics. In order to reduce the prescription of antibiotics, it has been researched the use of photodynamic therapy (PDT) as antimicrobial intervention, with great results. The objective of this work is to verify if the PDT interferes with the chemotaxis of neutrophils and T lymphocytes into the periodontal tissue. For such, 05 patients who had undergone prior treatment with periodontal good oral hygiene and periodontal pockets with residual surgical indication were allocated. PDT was applied under the following parameters: methylene blue (50 ??g/ml) was applied to the bottom of the periodontal pocket and after 5 minutes, via transmucosal, was irradiated with diode laser 660 nm, P = 100 mW, t = 90 s per point 9 J/point, energy density: 22 J/cm2, power density: 250 mW/cm2. One week after PDT, the surgical removal of the periodontal pockets was performed and the samples sent for routine histology and immunohistochemistry for detection of neutrophil and lymphocyte. The samples were photographed and positive cells counted with the aid of ImageJ software. The data were evaluated statistically and no significant difference between groups (Mann-Whitney p = 0.5). It was concluded that PDT did not interfere with the chemotaxis of neutrophils and T lymphocytes in the periodontal tissue after 7 days. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-12-16 |
dc.date.accessioned.fl_str_mv |
2016-05-19T15:00:56Z |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Mernick, Ana Paula de Souza. A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal. 2014. 52 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1312 |
identifier_str_mv |
Mernick, Ana Paula de Souza. A terapia fotodin??mica na quimiotaxia de neutr??filos e linf??citos T no tecido periodontal. 2014. 52 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/1312 |
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por |
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por |
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8765449414823306929 |
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Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de |
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UNINOVE |
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Brasil |
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Sa??de |
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Universidade Nove de Julho |
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