Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de
|
Departamento: |
Sa??de
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/1785 |
Resumo: | The overall recognition on the growth of lung inflammatory diseases has led to efforts to develop new therapies, particularly for the control of inflammation. Among the therapies used for disorders that affecting the lung, laser therapy has been highlighted, since it is a noninvasive therapy without side effects and anti-inflammatory and antioxidants actions. Studies have shown beneficial effects of low level laser therapy (LLLT) for the treatment of lung diseases, however, the operative mechanisms need to be better understood. Thus, lung inflammation models are important tools to extend the mechanistic knowledge of LLLT. Accordingly, we used Formaldehyde (FA), an environmental and occupational pollutant, which induces airway inflammation. FA is a chemical widely used in many industries, in anatomy laboratories, pathology, histology and is also expelled in cigarette smoke. Lung inflammation induced by exposure to FA is an interesting and important respiratory disease model. For this, we decided to investigate the effects of LLLT in the model of lung inflammation induced by FA, which is already well established. Our studies prioritized the analysis of cells recruited in the lung, blood and bone marrow, the enzyme myeloperoxidase activity, release and gene expression of cytokine, evaluation of lung vascular permeability and mast cell degranulation. Our results suggest a beneficial effect of LLLT on model of lung inflammation induced by pollutant; since the LLLT treatment significantly reduced the number of neutrophils recruited to the lung, lung vascular permeability, mast cell degranulationand also reduced the release of IL-6 and TNF levels in the bronchoalveolar lavage fluidassociated with increased release and gene expression of IL-10. This study may provide important information of a possible therapy to minimize the undesirable effects of pollutants on health. |
id |
NOVE_5a190d7c1825de540b6284d2e555294a |
---|---|
oai_identifier_str |
oai:localhost:tede/1785 |
network_acronym_str |
NOVE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
repository_id_str |
|
spelling |
Franco, Adriana Lino dos SantosChavantes, Maria CristinaFranco, Adriana Lino dos SantosOliveira, Ana Paula Ligeiro dePrado, Carla M??ximohttp://lattes.cnpq.br/2128996617976328Silva, Cristiane Miranda da2018-06-15T14:44:25Z2015-09-21Silva, Cristiane Miranda da. Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do. 2015. 30 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1785The overall recognition on the growth of lung inflammatory diseases has led to efforts to develop new therapies, particularly for the control of inflammation. Among the therapies used for disorders that affecting the lung, laser therapy has been highlighted, since it is a noninvasive therapy without side effects and anti-inflammatory and antioxidants actions. Studies have shown beneficial effects of low level laser therapy (LLLT) for the treatment of lung diseases, however, the operative mechanisms need to be better understood. Thus, lung inflammation models are important tools to extend the mechanistic knowledge of LLLT. Accordingly, we used Formaldehyde (FA), an environmental and occupational pollutant, which induces airway inflammation. FA is a chemical widely used in many industries, in anatomy laboratories, pathology, histology and is also expelled in cigarette smoke. Lung inflammation induced by exposure to FA is an interesting and important respiratory disease model. For this, we decided to investigate the effects of LLLT in the model of lung inflammation induced by FA, which is already well established. Our studies prioritized the analysis of cells recruited in the lung, blood and bone marrow, the enzyme myeloperoxidase activity, release and gene expression of cytokine, evaluation of lung vascular permeability and mast cell degranulation. Our results suggest a beneficial effect of LLLT on model of lung inflammation induced by pollutant; since the LLLT treatment significantly reduced the number of neutrophils recruited to the lung, lung vascular permeability, mast cell degranulationand also reduced the release of IL-6 and TNF levels in the bronchoalveolar lavage fluidassociated with increased release and gene expression of IL-10. This study may provide important information of a possible therapy to minimize the undesirable effects of pollutants on health.O reconhecimento global no crescimento de doen??as inflamat??rias pulmonares tem levado a esfor??os para o desenvolvimento de novas terapias, particularmente para o controle da inflama????o. Dentre as terapias usadas para patologias que acometem o pulm??o, a laserterapia tem merecido destaque, uma vez que ?? uma terapia n??o invasiva, sem efeitos colaterais e com a????es antiinflamat??rias e antioxidantes importantes. Estudos apontam para efeitos ben??ficos do laser de baixa intensidade (LBI) para o tratamento de doen??as pulmonares, no entanto, os mecanismos operantes necessitam ser melhor compreendidos. Assim, modelos de inflama????o pulmonar s??o importantes ferramentas para ampliar os conhecimentos mecanicistas do LBI. Nesse sentido, utilizamos o Formalde??do (FA), um poluente ambiental e ocupacional, para indu????o da inflama????o das vias a??reas. O FA ?? um agente qu??mico amplamente utilizado em diversas ind??strias, em laborat??rios de anatomia, patologia, histologia, emitido pela queima de combust??veis, pela queima do g??s de cozinha e tamb??m expelido na fuma??a do cigarro, o que o caracteriza como um poluente ambiental e ocupacional. A inflama????o pulmonar induzida pela exposi????o ao FA constitui um interessante e importante modelo de doen??a respirat??ria. Nesse sentido, resolvemos investigar os efeitos do LBI no modelo de inflama????o pulmonar induzido pelo FA o qual j?? est?? bem estabelecido. Nossos estudos priorizaram a an??lise de c??lulas presentes no pulm??o, sangue e medula ??ssea, atividade da enzima mieloperoxidase,libera????o e express??o g??nica de citocinas, avalia????o da permeabilidade vascular pulmonar e desgranula????o de mast??citos. Nossos resultados apontam para efeitos ben??ficos do LBI sobre modelo de inflama????o pulmonar induzida por poluente, uma vez que o tratamento com LBI reduziu significativamente o n??mero de neutr??filos recrutados para o pulm??o, a permeabilidade vascular pulmonar, desgranula????o de mast??citos e tamb??m reduziu a libera????o de IL-6 e TNF concomitantemente ao aumento na libera????o e express??o g??nica de IL-10. Assim, este estudo pode fornecer subs??dios importantes de uma poss??vel terapia para minimizar os efeitos indesej??veis dos poluentes na sa??de.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-15T14:44:25Z No. of bitstreams: 1 Cristiane Miranda da Silva.pdf: 605974 bytes, checksum: f05e2d4bf1f27e00cd1beccd0a8ffe3d (MD5)Made available in DSpace on 2018-06-15T14:44:25Z (GMT). No. of bitstreams: 1 Cristiane Miranda da Silva.pdf: 605974 bytes, checksum: f05e2d4bf1f27e00cd1beccd0a8ffe3d (MD5) Previous issue date: 2015-09-21application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??deUNINOVEBrasilSa??depolui????oinflama????o pulmonarmast??citoscitocinaspermeabilidade vascular pulmonarratospollutionlung inflammationmastocytescytokineslung vascular permeabilityratsCIENCIAS DA SAUDEEfeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??doEffect of lasertherapy on the lung inflammation induced by formaldehyde exposureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALCristiane Miranda da Silva.pdfCristiane Miranda da Silva.pdfapplication/pdf605974http://localhost:8080/tede/bitstream/tede/1785/2/Cristiane+Miranda+da+Silva.pdff05e2d4bf1f27e00cd1beccd0a8ffe3dMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1785/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/17852018-06-15 11:44:25.43oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T14:44:25Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
dc.title.alternative.eng.fl_str_mv |
Effect of lasertherapy on the lung inflammation induced by formaldehyde exposure |
title |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
spellingShingle |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do Silva, Cristiane Miranda da polui????o inflama????o pulmonar mast??citos citocinas permeabilidade vascular pulmonar ratos pollution lung inflammation mastocytes cytokines lung vascular permeability rats CIENCIAS DA SAUDE |
title_short |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
title_full |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
title_fullStr |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
title_full_unstemmed |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
title_sort |
Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do |
author |
Silva, Cristiane Miranda da |
author_facet |
Silva, Cristiane Miranda da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.advisor-co1.fl_str_mv |
Chavantes, Maria Cristina |
dc.contributor.referee1.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.referee2.fl_str_mv |
Oliveira, Ana Paula Ligeiro de |
dc.contributor.referee3.fl_str_mv |
Prado, Carla M??ximo |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2128996617976328 |
dc.contributor.author.fl_str_mv |
Silva, Cristiane Miranda da |
contributor_str_mv |
Franco, Adriana Lino dos Santos Chavantes, Maria Cristina Franco, Adriana Lino dos Santos Oliveira, Ana Paula Ligeiro de Prado, Carla M??ximo |
dc.subject.por.fl_str_mv |
polui????o inflama????o pulmonar mast??citos citocinas permeabilidade vascular pulmonar ratos |
topic |
polui????o inflama????o pulmonar mast??citos citocinas permeabilidade vascular pulmonar ratos pollution lung inflammation mastocytes cytokines lung vascular permeability rats CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
pollution lung inflammation mastocytes cytokines lung vascular permeability rats |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The overall recognition on the growth of lung inflammatory diseases has led to efforts to develop new therapies, particularly for the control of inflammation. Among the therapies used for disorders that affecting the lung, laser therapy has been highlighted, since it is a noninvasive therapy without side effects and anti-inflammatory and antioxidants actions. Studies have shown beneficial effects of low level laser therapy (LLLT) for the treatment of lung diseases, however, the operative mechanisms need to be better understood. Thus, lung inflammation models are important tools to extend the mechanistic knowledge of LLLT. Accordingly, we used Formaldehyde (FA), an environmental and occupational pollutant, which induces airway inflammation. FA is a chemical widely used in many industries, in anatomy laboratories, pathology, histology and is also expelled in cigarette smoke. Lung inflammation induced by exposure to FA is an interesting and important respiratory disease model. For this, we decided to investigate the effects of LLLT in the model of lung inflammation induced by FA, which is already well established. Our studies prioritized the analysis of cells recruited in the lung, blood and bone marrow, the enzyme myeloperoxidase activity, release and gene expression of cytokine, evaluation of lung vascular permeability and mast cell degranulation. Our results suggest a beneficial effect of LLLT on model of lung inflammation induced by pollutant; since the LLLT treatment significantly reduced the number of neutrophils recruited to the lung, lung vascular permeability, mast cell degranulationand also reduced the release of IL-6 and TNF levels in the bronchoalveolar lavage fluidassociated with increased release and gene expression of IL-10. This study may provide important information of a possible therapy to minimize the undesirable effects of pollutants on health. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-09-21 |
dc.date.accessioned.fl_str_mv |
2018-06-15T14:44:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Silva, Cristiane Miranda da. Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do. 2015. 30 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1785 |
identifier_str_mv |
Silva, Cristiane Miranda da. Efeito da laserterapia sobre a inflama????o pulmonar induzida pela exposi????o ao formalde??do. 2015. 30 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1785 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
instname_str |
Universidade Nove de Julho (UNINOVE) |
instacron_str |
UNINOVE |
institution |
UNINOVE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/1785/2/Cristiane+Miranda+da+Silva.pdf http://localhost:8080/tede/bitstream/tede/1785/1/license.txt |
bitstream.checksum.fl_str_mv |
f05e2d4bf1f27e00cd1beccd0a8ffe3d bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
_version_ |
1797250754651619328 |