Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o

Detalhes bibliográficos
Ano de defesa: 2010
Autor(a) principal: Pestana, Paulo Roberto Dantas lattes
Orientador(a): Ferrari, Raquel Agnelli Mesquita lattes
Banca de defesa: Nunes, Fabio Daumas lattes, Carvalho, Regiane Albertini de lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Nove de Julho
Programa de Pós-Graduação: Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
Departamento: Sa??de
País: BR
Palavras-chave em Português:
reparo
m??sculo
regenera????o
exerc??cio aqu??tico
Palavras-chave em Inglês:
repair
muscle
regeneration
aquatic exercise
Área do conhecimento CNPq:
CIENCIAS DA SAUDE
Link de acesso: http://bibliotecatede.uninove.br/tede/handle/tede/835
Resumo: Skeletal muscles are dynamic tissues that can alter their phenotypic characteristics, providing a better functional adaptation faced with various stimuli. OBJECTIVE: To investigate the influence of aquatic exercise on the expression of myogenic markers, during regeneration of skeletal muscle of rats after cryoinjury. METHODS: A total of 60 rats were divided randomly into six groups are: (1) Control (n = 5), (2) sham control (n = 5), (3) Group adaptation (n = 5) and two experimental groups sacrificed after 7, 14 and 21 days (4) cryoinjuryed and treated with aquatic exercise (n = 15), (5) cryoinjuryed and untreated (n = 15) and (6) without injury and submitted to aquatic exercise (n = 15). The aquatic exercise sessions were conducted 6 times a week and each session lasted for 90min. At the end of the protocol the animals were sacrificed and total mRNA was extracted for analysis of MyoD and myogenin and morphological analysis of the lesion. RESULTS: The results revealed an increase in MyoD mRNA 7 days swimming group when compared to other groups. With respect to the expression of myogenin was found a significant increase after 14 days in the swimming group compared to group the same period with the injury and 7 days groups. 14 days swimming group in the expression of myogenin showed similar to the control and sham groups, no statistically significant differences were found. Regarding the morphology showed that the muscles of the control group showed normal histological aspect. The sham group showed edema, myonecrosis and inflammatory infiltration in grade 1. In groups 7, 14 and 21 days, there were no statistically significant differences in four stages of tissue remodeling evaluated (inflammatory infiltration, edema, necrosis and immature muscle fibers) between the injured groups compared to groups with lesions and water treatment CONCLUSION: The aquatic exercise induced changes in the expression of myogenic markers during the repair process of skeletal muscle of rats after cryoinjury.
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spelling Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Martins, Manoela DominguesCPF:00000000000http://lattes.cnpq.br/4958184722611235Fernandes, Kristiane Santos PortaNunes, Fabio Daumashttp://lattes.cnpq.br/4909755821591847Carvalho, Regiane Albertini dehttp://lattes.cnpq.br/2515657728339113CPF:33923112866http://lattes.cnpq.br/1236095402289436Pestana, Paulo Roberto Dantas2015-04-22T17:20:28Z2011-07-052010-12-16PESTANA, Paulo Roberto Dantas. Effect of the aquatic exercise in the regeneration of the esquel??tico muscle after crioles??o .. 2010. 68 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2010.http://bibliotecatede.uninove.br/tede/handle/tede/835Skeletal muscles are dynamic tissues that can alter their phenotypic characteristics, providing a better functional adaptation faced with various stimuli. OBJECTIVE: To investigate the influence of aquatic exercise on the expression of myogenic markers, during regeneration of skeletal muscle of rats after cryoinjury. METHODS: A total of 60 rats were divided randomly into six groups are: (1) Control (n = 5), (2) sham control (n = 5), (3) Group adaptation (n = 5) and two experimental groups sacrificed after 7, 14 and 21 days (4) cryoinjuryed and treated with aquatic exercise (n = 15), (5) cryoinjuryed and untreated (n = 15) and (6) without injury and submitted to aquatic exercise (n = 15). The aquatic exercise sessions were conducted 6 times a week and each session lasted for 90min. At the end of the protocol the animals were sacrificed and total mRNA was extracted for analysis of MyoD and myogenin and morphological analysis of the lesion. RESULTS: The results revealed an increase in MyoD mRNA 7 days swimming group when compared to other groups. With respect to the expression of myogenin was found a significant increase after 14 days in the swimming group compared to group the same period with the injury and 7 days groups. 14 days swimming group in the expression of myogenin showed similar to the control and sham groups, no statistically significant differences were found. Regarding the morphology showed that the muscles of the control group showed normal histological aspect. The sham group showed edema, myonecrosis and inflammatory infiltration in grade 1. In groups 7, 14 and 21 days, there were no statistically significant differences in four stages of tissue remodeling evaluated (inflammatory infiltration, edema, necrosis and immature muscle fibers) between the injured groups compared to groups with lesions and water treatment CONCLUSION: The aquatic exercise induced changes in the expression of myogenic markers during the repair process of skeletal muscle of rats after cryoinjury.Os m??sculos esquel??ticos s??o tecidos din??micos que podem alterar suas caracter??sticas fenot??picas, proporcionando uma melhor adapta????o funcional frente a est??mulos variados. OBJETIVO: Investigar a influ??ncia do exerc??cio aqu??tico sobre a express??o de marcadores miog??nicos, durante a regenera????o do m??sculo esquel??tico de rato ap??s crioles??o. METODOLOGIA: Foram utilizados 60 ratos divididos aleatoriamente em 6 grupos sendo: (1) Controle (n=5); (2) Grupo sham (n=5), (3) Grupo adapta????o (n=5) e 3 grupos experimentais sacrificados ap??s 7, 14 e 21 dias e denominados (4) criolesionado e tratado com exerc??cio aqu??tico (n=15); (5) criolesionado sem tratamento aqu??tico (pata contralateral foi utilizada como controle interno) (n=15) e (6) somente com exerc??cio aqu??tico (n=15). As sess??es de exerc??cio aqu??tico foram realizadas 6 vezes por semana com a dura????o de 90min cada sess??o. Ao t??rmino do protocolo os animais foram sacrificados e foram extra??dos o RNAm total para an??lise da express??o de MyoD e miogenina e a an??lise morfol??gica da les??o. RESULTADOS: Os resultados evidenciaram aumento do RNAm de MyoD no grupo 7 dias nata????o quando comparado aos demais grupos experimentais. Com rela????o ?? express??o de miogenina foi encontrado um aumento significativo ap??s 14 dias no grupo nata????o com rela????o ao grupo do mesmo per??odo com les??o e aos grupos 7 dias. No grupo 14 dias nata????o a express??o de miogenina apresentou valores pr??ximos aos grupos controle e sham, n??o sendo verificadas diferen??as estatisticamente. Em rela????o aos aspectos morfol??gicos permitiu verificar que os m??sculos do grupo controle apresentaram aspecto histol??gico normal. O grupo sham apresentou edema, mionecrose e infiltrado inflamat??rio em grau 1. Nos grupos 7, 14 e 21 dias, n??o existiram diferen??as estatisticamente significativas nas 4 etapas de remodelamento tecidual avaliadas (infiltrado inflamat??rio, edema, necrose e fibras musculares imaturas) entre os grupos lesionados quando comparados aos grupos com les??o e tratamento aqu??tico .CONCLUS??O: O exerc??cio aqu??tico induziu altera????es na express??o de marcadores miog??nicos e n??o causou altera????es morfol??gicas durante o reparo do m??sculo esquel??tico ap??s crioles??o.Made available in DSpace on 2015-04-22T17:20:28Z (GMT). No. of bitstreams: 1 B_Paulo Roberto Dantas Pestana.pdf: 669339 bytes, checksum: 59d7a28874c670adb77fc1aa355e3139 (MD5) Previous issue date: 2010-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??dereparom??sculoregenera????oexerc??cio aqu??ticorepairmuscleregenerationaquatic exerciseCIENCIAS DA SAUDEEfeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??oEffect of the aquatic exercise in the regeneration of the esquel??tico muscle after crioles??oinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALB_Paulo Roberto Dantas Pestana.pdfapplication/pdf669339http://localhost:8080/tede/bitstream/tede/835/1/B_Paulo+Roberto+Dantas+Pestana.pdf59d7a28874c670adb77fc1aa355e3139MD51tede/8352018-06-15 12:46:21.826oai:localhost:tede/835Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:46:21Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
dc.title.alternative.eng.fl_str_mv Effect of the aquatic exercise in the regeneration of the esquel??tico muscle after crioles??o
title Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
spellingShingle Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
Pestana, Paulo Roberto Dantas
reparo
m??sculo
regenera????o
exerc??cio aqu??tico
repair
muscle
regeneration
aquatic exercise
CIENCIAS DA SAUDE
title_short Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
title_full Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
title_fullStr Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
title_full_unstemmed Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
title_sort Efeito do exerc??cio aqu??tico na regenera????o do m??sculo esquel??tico ap??s crioles??o
author Pestana, Paulo Roberto Dantas
author_facet Pestana, Paulo Roberto Dantas
author_role author
dc.contributor.advisor1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7048141683554943
dc.contributor.advisor-co1.fl_str_mv Martins, Manoela Domingues
dc.contributor.advisor-co1ID.fl_str_mv CPF:00000000000
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/4958184722611235
dc.contributor.advisor-co2.fl_str_mv Fernandes, Kristiane Santos Porta
dc.contributor.referee1.fl_str_mv Nunes, Fabio Daumas
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/4909755821591847
dc.contributor.referee2.fl_str_mv Carvalho, Regiane Albertini de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/2515657728339113
dc.contributor.authorID.fl_str_mv CPF:33923112866
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1236095402289436
dc.contributor.author.fl_str_mv Pestana, Paulo Roberto Dantas
contributor_str_mv Ferrari, Raquel Agnelli Mesquita
Martins, Manoela Domingues
Fernandes, Kristiane Santos Porta
Nunes, Fabio Daumas
Carvalho, Regiane Albertini de
dc.subject.por.fl_str_mv reparo
m??sculo
regenera????o
exerc??cio aqu??tico
topic reparo
m??sculo
regenera????o
exerc??cio aqu??tico
repair
muscle
regeneration
aquatic exercise
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv repair
muscle
regeneration
aquatic exercise
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description Skeletal muscles are dynamic tissues that can alter their phenotypic characteristics, providing a better functional adaptation faced with various stimuli. OBJECTIVE: To investigate the influence of aquatic exercise on the expression of myogenic markers, during regeneration of skeletal muscle of rats after cryoinjury. METHODS: A total of 60 rats were divided randomly into six groups are: (1) Control (n = 5), (2) sham control (n = 5), (3) Group adaptation (n = 5) and two experimental groups sacrificed after 7, 14 and 21 days (4) cryoinjuryed and treated with aquatic exercise (n = 15), (5) cryoinjuryed and untreated (n = 15) and (6) without injury and submitted to aquatic exercise (n = 15). The aquatic exercise sessions were conducted 6 times a week and each session lasted for 90min. At the end of the protocol the animals were sacrificed and total mRNA was extracted for analysis of MyoD and myogenin and morphological analysis of the lesion. RESULTS: The results revealed an increase in MyoD mRNA 7 days swimming group when compared to other groups. With respect to the expression of myogenin was found a significant increase after 14 days in the swimming group compared to group the same period with the injury and 7 days groups. 14 days swimming group in the expression of myogenin showed similar to the control and sham groups, no statistically significant differences were found. Regarding the morphology showed that the muscles of the control group showed normal histological aspect. The sham group showed edema, myonecrosis and inflammatory infiltration in grade 1. In groups 7, 14 and 21 days, there were no statistically significant differences in four stages of tissue remodeling evaluated (inflammatory infiltration, edema, necrosis and immature muscle fibers) between the injured groups compared to groups with lesions and water treatment CONCLUSION: The aquatic exercise induced changes in the expression of myogenic markers during the repair process of skeletal muscle of rats after cryoinjury.
publishDate 2010
dc.date.issued.fl_str_mv 2010-12-16
dc.date.available.fl_str_mv 2011-07-05
dc.date.accessioned.fl_str_mv 2015-04-22T17:20:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PESTANA, Paulo Roberto Dantas. Effect of the aquatic exercise in the regeneration of the esquel??tico muscle after crioles??o .. 2010. 68 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2010.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/tede/handle/tede/835
identifier_str_mv PESTANA, Paulo Roberto Dantas. Effect of the aquatic exercise in the regeneration of the esquel??tico muscle after crioles??o .. 2010. 68 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2010.
url http://bibliotecatede.uninove.br/tede/handle/tede/835
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language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
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