Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno
Ano de defesa: | 2014 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
|
Departamento: |
Sa??de
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/tede/handle/tede/906 |
Resumo: | Envenoming induced by Bothrops snakes is characterized by drastic local tissue damage involving hemorrhage, myonecrosis and proeminent inflammatory and hyperalgesic response. The most effective treatment is antivenom therapy, which is ineffective in neutralizing the local response. Herein, it was evaluated the effectiveness of light-emitting diode (LED) at wavelengths of 635 and 945 nm in reducing inflammatory hyperalgesia induced by Bothrops moojeni venom (BmV) or myotoxins (MjTX-I and -II) isolated from this venom. LBP was irradiated at a wavelength of 685 nm and 2.2 J/cm2 energy density, red LED in wavelength of 635 nm and 4 J/cm2 energy density and infrared LED in 945 nm wavelength and energy 4 J/cm2 density. The effect of LBP and LED were evaluated when applied alone or in combination with the antivenom. Our results demonstrated that both LBP as well as LED reduced the hiperalgesic effect induced by the B. moojeni venom alone or in combination with the antivenon treatment. We note that the LBP was effective in reducing levels of mRNA transcript that translates the pro-inflammatory cytokines IL-1 ??, IL-6, and TNF-??, as well as receptors for bradykinin B1 and B2. Still, the LBP was able to increase the expression of anti-inflammatory cytokine IL-10, on muscle plant of mice. Treatment with LBP and LED reduced edema formation induced by MjTX MjTX-I and-II in a similar manner. The combination of antivenom with the LED did not improve the effect of low-level therapy alone, in reducing the edema formation induced by MjTX-I or II. The results obtained in this study have shown that LBP and LED in the parameters used can be an alternative for the treatment of the local effects caused by bothropic venom accidents as well as an important tool for studying the mechanisms involved in the inflammatory process induced by poisons or toxins. |
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Zamuner, Stella Reginahttp://lattes.cnpq.br/1935805744318404Costa, Maricilia Silvahttp://lattes.cnpq.br/7963390052508784Marcos, Rodrigo Labathttp://lattes.cnpq.br/2964112163504080Carvalho, Paulo de Tarso Camillo dehttp://lattes.cnpq.br/2378784910179477Vessoni, Sandra Coccuzzo Sampaiohttp://lattes.cnpq.br/6181892645004033CPF:22194023801http://lattes.cnpq.br/5155813826833325Andrade, Nikele Nadur2015-04-22T17:20:46Z2014-12-022014-02-26ANDRADE, Nikele Nadur. Efeito da Fotobioestimula????o no edema e hiperalgesia induzido pelo veneno de Bothrops moojeni e por duas miotoxinas isoladas deste veneno. 2014. 118 f. Tese (Doutorado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2014.http://bibliotecatede.uninove.br/tede/handle/tede/906Envenoming induced by Bothrops snakes is characterized by drastic local tissue damage involving hemorrhage, myonecrosis and proeminent inflammatory and hyperalgesic response. The most effective treatment is antivenom therapy, which is ineffective in neutralizing the local response. Herein, it was evaluated the effectiveness of light-emitting diode (LED) at wavelengths of 635 and 945 nm in reducing inflammatory hyperalgesia induced by Bothrops moojeni venom (BmV) or myotoxins (MjTX-I and -II) isolated from this venom. LBP was irradiated at a wavelength of 685 nm and 2.2 J/cm2 energy density, red LED in wavelength of 635 nm and 4 J/cm2 energy density and infrared LED in 945 nm wavelength and energy 4 J/cm2 density. The effect of LBP and LED were evaluated when applied alone or in combination with the antivenom. Our results demonstrated that both LBP as well as LED reduced the hiperalgesic effect induced by the B. moojeni venom alone or in combination with the antivenon treatment. We note that the LBP was effective in reducing levels of mRNA transcript that translates the pro-inflammatory cytokines IL-1 ??, IL-6, and TNF-??, as well as receptors for bradykinin B1 and B2. Still, the LBP was able to increase the expression of anti-inflammatory cytokine IL-10, on muscle plant of mice. Treatment with LBP and LED reduced edema formation induced by MjTX MjTX-I and-II in a similar manner. The combination of antivenom with the LED did not improve the effect of low-level therapy alone, in reducing the edema formation induced by MjTX-I or II. The results obtained in this study have shown that LBP and LED in the parameters used can be an alternative for the treatment of the local effects caused by bothropic venom accidents as well as an important tool for studying the mechanisms involved in the inflammatory process induced by poisons or toxins.O envenenamento induzido por serpentes do g??nero Bothrops ?? caracterizada por danos no tecido local envolvendo hemorragia, mionecrose e uma resposta inflamat??ria e hiperalg??sica de instala????o r??pida e intensa. O tratamento mais eficaz ?? a terapia de antiveneno, capaz de reverter os sintomas sist??micos, por??m ?? ineficaz em neutralizar a resposta local. Neste estudo foi avaliado o efeito da fotobioestimula????o com o laser de baixa pot??ncia (LBP) e o light emitting diode (LED) no edema e hiperalgesia induzido pelo veneno de B. moojeni e duas mitoxinas isoladas deste veneno a MjTX-I e -II. Foi utilizado o LBP no comprimento de onda de 685 nm e densidade de energia de 2,2 J/cm2, do LEDs vermelho com comprimento de onda de 635 nm, e densidade de energia de 4 J/cm2 e LED infravermelho no comprimento de onda 945 nm e densidade de energia de 4 J/cm2. O efeito do LBP e LED foram avaliados quando aplicados sozinho ou conjuntamente com o antiveneno. Nossos resultados demonstraram que tanto o LBP quanto o LED reduziram o efeito hiperalg??sico induzido pelo veneno de B. moojeni sozinho ou em combina????o com o tratamento do antiveneno. Verificamos que o LBP foi eficaz em reduzir n??veis de transcri????o de RNAm que traduz as citocinas pr??-inflamat??rias IL-1??, IL-6, e TNF-??, assim como os receptores de bradicinina B1 e B2. Ainda, o LBP foi capaz de aumentar a express??o da citocina anti-inflamat??ria IL-10, no m??sculo plantar de camundongos. O tratamento com o LBP e LED reduziu o edema podal induzidos pela MjTX-I e MjTX-II de maneira semelhante. A combina????o do soro antibotr??pico com o LED n??o melhorou o efeito da terapia de baixa pot??ncia sozinha, na redu????o da forma????o de edema podal induzido pela MjTX-I ou II. Os resultados obtidos neste trabalho mostraram que o LBP e o LED nos par??metros utilizados possam ser uma alternativa para o tratamento dos efeitos locais dos acidentes causados por serpentes botr??picas assim como uma importante ferramenta para se estudar os mecanismos envolvidos no processo inflamat??rio induzido por venenos ou toxinas.Made available in DSpace on 2015-04-22T17:20:46Z (GMT). No. of bitstreams: 1 Nikele Nadur Andrade.pdf: 1640441 bytes, checksum: 9e33aac545f16e43b61d08bfd500560e (MD5) Previous issue date: 2014-02-26application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??debothrops moojenimiotoxinador inflamat??riaedemalaser de baixa pot??nciaLEDbothrops moojenimyotoxininflammatory painedemalow level laserLEDCIENCIAS DA SAUDEEfeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste venenoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALNikele Nadur Andrade.pdfapplication/pdf1640441http://localhost:8080/tede/bitstream/tede/906/1/Nikele+Nadur+Andrade.pdf9e33aac545f16e43b61d08bfd500560eMD51tede/9062018-06-15 12:41:43.746oai:localhost:tede/906Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:41:43Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
title |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
spellingShingle |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno Andrade, Nikele Nadur bothrops moojeni miotoxina dor inflamat??ria edema laser de baixa pot??ncia LED bothrops moojeni myotoxin inflammatory pain edema low level laser LED CIENCIAS DA SAUDE |
title_short |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
title_full |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
title_fullStr |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
title_full_unstemmed |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
title_sort |
Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno |
author |
Andrade, Nikele Nadur |
author_facet |
Andrade, Nikele Nadur |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Zamuner, Stella Regina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1935805744318404 |
dc.contributor.referee1.fl_str_mv |
Costa, Maricilia Silva |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7963390052508784 |
dc.contributor.referee2.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2964112163504080 |
dc.contributor.referee3.fl_str_mv |
Carvalho, Paulo de Tarso Camillo de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2378784910179477 |
dc.contributor.referee4.fl_str_mv |
Vessoni, Sandra Coccuzzo Sampaio |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/6181892645004033 |
dc.contributor.authorID.fl_str_mv |
CPF:22194023801 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5155813826833325 |
dc.contributor.author.fl_str_mv |
Andrade, Nikele Nadur |
contributor_str_mv |
Zamuner, Stella Regina Costa, Maricilia Silva Marcos, Rodrigo Labat Carvalho, Paulo de Tarso Camillo de Vessoni, Sandra Coccuzzo Sampaio |
dc.subject.por.fl_str_mv |
bothrops moojeni miotoxina dor inflamat??ria edema laser de baixa pot??ncia LED |
topic |
bothrops moojeni miotoxina dor inflamat??ria edema laser de baixa pot??ncia LED bothrops moojeni myotoxin inflammatory pain edema low level laser LED CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
bothrops moojeni myotoxin inflammatory pain edema low level laser LED |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Envenoming induced by Bothrops snakes is characterized by drastic local tissue damage involving hemorrhage, myonecrosis and proeminent inflammatory and hyperalgesic response. The most effective treatment is antivenom therapy, which is ineffective in neutralizing the local response. Herein, it was evaluated the effectiveness of light-emitting diode (LED) at wavelengths of 635 and 945 nm in reducing inflammatory hyperalgesia induced by Bothrops moojeni venom (BmV) or myotoxins (MjTX-I and -II) isolated from this venom. LBP was irradiated at a wavelength of 685 nm and 2.2 J/cm2 energy density, red LED in wavelength of 635 nm and 4 J/cm2 energy density and infrared LED in 945 nm wavelength and energy 4 J/cm2 density. The effect of LBP and LED were evaluated when applied alone or in combination with the antivenom. Our results demonstrated that both LBP as well as LED reduced the hiperalgesic effect induced by the B. moojeni venom alone or in combination with the antivenon treatment. We note that the LBP was effective in reducing levels of mRNA transcript that translates the pro-inflammatory cytokines IL-1 ??, IL-6, and TNF-??, as well as receptors for bradykinin B1 and B2. Still, the LBP was able to increase the expression of anti-inflammatory cytokine IL-10, on muscle plant of mice. Treatment with LBP and LED reduced edema formation induced by MjTX MjTX-I and-II in a similar manner. The combination of antivenom with the LED did not improve the effect of low-level therapy alone, in reducing the edema formation induced by MjTX-I or II. The results obtained in this study have shown that LBP and LED in the parameters used can be an alternative for the treatment of the local effects caused by bothropic venom accidents as well as an important tool for studying the mechanisms involved in the inflammatory process induced by poisons or toxins. |
publishDate |
2014 |
dc.date.available.fl_str_mv |
2014-12-02 |
dc.date.issued.fl_str_mv |
2014-02-26 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ANDRADE, Nikele Nadur. Efeito da Fotobioestimula????o no edema e hiperalgesia induzido pelo veneno de Bothrops moojeni e por duas miotoxinas isoladas deste veneno. 2014. 118 f. Tese (Doutorado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2014. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/906 |
identifier_str_mv |
ANDRADE, Nikele Nadur. Efeito da Fotobioestimula????o no edema e hiperalgesia induzido pelo veneno de Bothrops moojeni e por duas miotoxinas isoladas deste veneno. 2014. 118 f. Tese (Doutorado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2014. |
url |
http://bibliotecatede.uninove.br/tede/handle/tede/906 |
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por |
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por |
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8765449414823306929 |
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600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Nove de Julho |
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Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
dc.publisher.initials.fl_str_mv |
UNINOVE |
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BR |
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Sa??de |
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Universidade Nove de Julho |
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Biblioteca Digital de Teses e Dissertações da Uninove |
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