Efeito do laser de baixa pot??ncia sobre c??lulas endoteliais tEND submetidas ?? les??o por veneno da serpente bothropoides jararaca

• Ano de defesa: 2013
• Autor(a) principal: Franco, Ana Tereza Barufi
• Orientador(a): Zamuner, Stella Regina
• Banca de defesa: Silva, Carlos Alberto, Silva J??nior, Jos?? Antonio
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Nove de Julho
• Programa de Pós-Graduação: Programa de Mestrado em Medicina
• Departamento: Sa??de
• País: Brasil
• Palavras-chave em Português: endot??lio; bothropoides jararaca; laser de baixa pot??ncia
• Palavras-chave em Inglês: endothelium; bothropoides jararaca; low level laser
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: http://bibliotecatede.uninove.br/handle/tede/1145

The Bothropoides and Bothrops gender are responsible for the largest number of snakebites in Brazil, presenting serious systemic effects that induce pathophysiological alterations characterized by local reactions such as edema , myonecrosis, pain and hemohrrage. The antivenom is used to neutralize the greatest possible amount of circulating venom, minimizing its systemic effects. But its action does not extend to local manifestations. The low level laser therapy is an alternative treatment in situations of local lesions due to its biological effects, and is considered a biostimulate resource to the tissues . The aim of this study was to analyze the effect of low power laser -red wavelength of 660 nm, and the infrared laser- wavelength 780 nm, in endothelial cells submitted to injury by Bothropoides jararaca venom. The results showed that the snake venom affected the integrity of endothelial, in all concentrations (1, 5, 10, 12,5, 25 and 50 ??g/mL); and affected the viability of endothelial cell in 10 ??g/ mL at all time periods analyzed . To evaluate the protective effect of the laser, the dose of 4 J/cm2 reduced the detachment of endothelial cells by improving cellular integrity . This effect was observed in a wavelength of 660 nm at 60 and 120 minutes. The same effect was not observed with the wavelength of 780 nm. So, the present study allowed us to better understand the pathophysiological effects of Bothropoides venom, and contributed to a better understanding of the effects of low power laser and eventually facilitate therapeutic measures more efficient and / or complementary.