Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd)
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de Mestrado em Medicina
|
Departamento: |
Sa??de
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/1149 |
Resumo: | The scorpionism is considered a public health worldwide, with Tityus serrulatus the main cause of accidents in Brazil. Symptoms of envenomation by Tityus serrulatus ranging from local pain to severe systemic reactions such as cardiac dysfunction and pulmonary edema, which is the leading cause of death. The amount of venom injected the age, physical and genetic factors of the victims are related to the severity of the symptoms presented by patients. Endothelial cells under physiological conditions play a protective role in the cardiovascular system. In the case of dysfunction or injury of that tissue occurs repercussion in the vascular structure, surrounding tissue, and finally on the cardiovascular system. In bronchial epithelial cells after injury, occurs repair mechanism mediated by cytokines and growth factors also occur increase in cell proliferation and differentiation after epithelial damage. Thus, this study aims to evaluate the modulating effect of Tityus serrulatus scorpion venom on endothelial cells and bronchial epithelial cells analyzing the viability of these cells and the mechanism by which inflammation occurs. Therefore, the cells in this study are cultured and incubated in culture plates with Tityus serrulatus scorpion venom in different concentrations and periods of time. The inflammatory mechanism was assessed through the measurement of cytokines in the supernatant of these cells. The results showed that Tityus serrulatus venom changed the viability of monolayers of endothelial cells (tEnd), and bronchial epithelial cells (BEAS), and this effect was more marked in bronchial epithelial cells. The results also demonstrate that the venom induces the release of cytokines IL-1???, IL-6 and IL-8. iii This study extend the knowledge of the actions of the venom of the scorpion Tityus serrulatus on bronchial epithelial and endothelial cells thus demonstrating changes in pulmonary physiology that may contribute to a better treatment strategy of scorpion envenomation. |
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Zamuner, Stella Reginahttp://lattes.cnpq.br/1935805744318404Vieira, Rodolfo de Paulahttp://lattes.cnpq.br/9213556008468472Silva, CaDell??, Humbertohttp://lattes.cnpq.br/7435662740477057Nascimento, Telma Lisboa dohttp://lattes.cnpq.br/857749293878641611214434860http://lattes.cnpq.br/6592633063730058Rigoni, Vera Lucia Silva2015-07-27T14:08:11Z2013-12-13Rigoni, Vera Lucia Silva. Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd). 2013. 53 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo .http://bibliotecatede.uninove.br/handle/tede/1149The scorpionism is considered a public health worldwide, with Tityus serrulatus the main cause of accidents in Brazil. Symptoms of envenomation by Tityus serrulatus ranging from local pain to severe systemic reactions such as cardiac dysfunction and pulmonary edema, which is the leading cause of death. The amount of venom injected the age, physical and genetic factors of the victims are related to the severity of the symptoms presented by patients. Endothelial cells under physiological conditions play a protective role in the cardiovascular system. In the case of dysfunction or injury of that tissue occurs repercussion in the vascular structure, surrounding tissue, and finally on the cardiovascular system. In bronchial epithelial cells after injury, occurs repair mechanism mediated by cytokines and growth factors also occur increase in cell proliferation and differentiation after epithelial damage. Thus, this study aims to evaluate the modulating effect of Tityus serrulatus scorpion venom on endothelial cells and bronchial epithelial cells analyzing the viability of these cells and the mechanism by which inflammation occurs. Therefore, the cells in this study are cultured and incubated in culture plates with Tityus serrulatus scorpion venom in different concentrations and periods of time. The inflammatory mechanism was assessed through the measurement of cytokines in the supernatant of these cells. The results showed that Tityus serrulatus venom changed the viability of monolayers of endothelial cells (tEnd), and bronchial epithelial cells (BEAS), and this effect was more marked in bronchial epithelial cells. The results also demonstrate that the venom induces the release of cytokines IL-1???, IL-6 and IL-8. iii This study extend the knowledge of the actions of the venom of the scorpion Tityus serrulatus on bronchial epithelial and endothelial cells thus demonstrating changes in pulmonary physiology that may contribute to a better treatment strategy of scorpion envenomation.O escorpionismo ?? considerado um problema mundial de sa??de p??blica, sendo a esp??cie Tityus serrulatus a principal causadora de acidentes graves no Brasil. Os sintomas do envenenamento pelo escorpi??o Tityus serrulatus v??o desde dor local at?? rea????es sist??micas severas, como disfun????o card??aca e edema pulmonar agudo, sendo este a principal causa de morte. A quantidade de veneno inoculado, idade, condi????es f??sicas e fatores gen??ticos das v??timas relacionam-se com a gravidade dos sintomas apresentados pelos pacientes. As c??lulas endoteliais, em condi????es fisiol??gicas, desempenham papel protetor do sistema cardiovascular. Em caso de disfun????o ou les??o desse tecido h?? repercuss??o sobre a estrutura vascular, tecido adjacente e, por fim sobre o sistema cardiovascular. J??, nas c??lulas epiteliais br??nquicas ap??s inj??ria, ocorre mecanismo de reparo mediado por citocinas e fatores de crescimento, ocorrendo tamb??m prolifera????o e diferencia????o celular ap??s o dano epitelial. Assim, o presente estudo tem como objetivo avaliar o efeito modulador do veneno do escorpi??o Tityus serrulatus em c??lulas endoteliais e c??lulas epiteliais br??nquicas, analisando a viabilidade destas c??lulas e o mecanismo de inflama????o pela qual s??o acometidas. Para tanto, as c??lulas em estudo foram cultivadas e incubadas em placas de cultura com o veneno do escorpi??o Tityus serrulatus, em diferentes concentra????es e em diferentes per??odos de tempo. O mecanismo inflamat??rio foi avaliado atrav??s da dosagem de citocinas no sobrenadante destas c??lulas. Os resultados demonstraram que o veneno de Tityus serrulatus alterou a viabilidade de i monocamadas das c??lulas epiteliais br??nquicas e c??lulas endoteliais, sendo que esse efeito foi mais evidente nas c??lulas epiteliais br??nquicas. Ainda, os resultados demonstraram que o veneno induz a libera????o das citocinas IL-1???, IL-6 e IL-8. Estes estudos ampliam o conhecimento das a????es do veneno do escorpi??o Tityus serrulatus nas c??lulas endoteliais (tEnd) e epiteliais br??nquicas (BEAS), comprovando assim altera????es na fisiologia pulmonar que poder??o contribuir para uma melhor estrat??gia de tratamento do envenenamento escorpi??nico.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2015-07-27T14:08:11Z No. of bitstreams: 1 Vera Lucia Silva Rigoni.pdf: 1348554 bytes, checksum: 28c01d5b4e69fddd38792d76d20fcf02 (MD5)Made available in DSpace on 2015-07-27T14:08:11Z (GMT). No. of bitstreams: 1 Vera Lucia Silva Rigoni.pdf: 1348554 bytes, checksum: 28c01d5b4e69fddd38792d76d20fcf02 (MD5) Previous issue date: 2013-12-13application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSa??dec??lulas epiteliais br??nquicasveneno Tityus serrulatusescorpi??ocitocinasinflama????oc??lulas endoteliaisbronchial epithelial cellsTityus serrulatus venomscorpioncytokinesinflammationendothelial cellsCIENCIAS DA SAUDEEfeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALVera Lucia Silva Rigoni.pdfVera Lucia Silva Rigoni.pdfapplication/pdf1348554http://localhost:8080/tede/bitstream/tede/1149/2/Vera+Lucia+Silva+Rigoni.pdf28c01d5b4e69fddd38792d76d20fcf02MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/tede/1149/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/11492017-05-26 18:25:08.591oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2017-05-26T21:25:08Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
title |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
spellingShingle |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) Rigoni, Vera Lucia Silva c??lulas epiteliais br??nquicas veneno Tityus serrulatus escorpi??o citocinas inflama????o c??lulas endoteliais bronchial epithelial cells Tityus serrulatus venom scorpion cytokines inflammation endothelial cells CIENCIAS DA SAUDE |
title_short |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
title_full |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
title_fullStr |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
title_full_unstemmed |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
title_sort |
Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd) |
author |
Rigoni, Vera Lucia Silva |
author_facet |
Rigoni, Vera Lucia Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Zamuner, Stella Regina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1935805744318404 |
dc.contributor.advisor-co1.fl_str_mv |
Vieira, Rodolfo de Paula |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9213556008468472 |
dc.contributor.referee1.fl_str_mv |
Silva, Ca |
dc.contributor.referee2.fl_str_mv |
Dell??, Humberto |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7435662740477057 |
dc.contributor.referee3.fl_str_mv |
Nascimento, Telma Lisboa do |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8577492938786416 |
dc.contributor.authorID.fl_str_mv |
11214434860 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6592633063730058 |
dc.contributor.author.fl_str_mv |
Rigoni, Vera Lucia Silva |
contributor_str_mv |
Zamuner, Stella Regina Vieira, Rodolfo de Paula Silva, Ca Dell??, Humberto Nascimento, Telma Lisboa do |
dc.subject.por.fl_str_mv |
c??lulas epiteliais br??nquicas veneno Tityus serrulatus escorpi??o citocinas inflama????o c??lulas endoteliais |
topic |
c??lulas epiteliais br??nquicas veneno Tityus serrulatus escorpi??o citocinas inflama????o c??lulas endoteliais bronchial epithelial cells Tityus serrulatus venom scorpion cytokines inflammation endothelial cells CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
bronchial epithelial cells Tityus serrulatus venom scorpion cytokines inflammation endothelial cells |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The scorpionism is considered a public health worldwide, with Tityus serrulatus the main cause of accidents in Brazil. Symptoms of envenomation by Tityus serrulatus ranging from local pain to severe systemic reactions such as cardiac dysfunction and pulmonary edema, which is the leading cause of death. The amount of venom injected the age, physical and genetic factors of the victims are related to the severity of the symptoms presented by patients. Endothelial cells under physiological conditions play a protective role in the cardiovascular system. In the case of dysfunction or injury of that tissue occurs repercussion in the vascular structure, surrounding tissue, and finally on the cardiovascular system. In bronchial epithelial cells after injury, occurs repair mechanism mediated by cytokines and growth factors also occur increase in cell proliferation and differentiation after epithelial damage. Thus, this study aims to evaluate the modulating effect of Tityus serrulatus scorpion venom on endothelial cells and bronchial epithelial cells analyzing the viability of these cells and the mechanism by which inflammation occurs. Therefore, the cells in this study are cultured and incubated in culture plates with Tityus serrulatus scorpion venom in different concentrations and periods of time. The inflammatory mechanism was assessed through the measurement of cytokines in the supernatant of these cells. The results showed that Tityus serrulatus venom changed the viability of monolayers of endothelial cells (tEnd), and bronchial epithelial cells (BEAS), and this effect was more marked in bronchial epithelial cells. The results also demonstrate that the venom induces the release of cytokines IL-1???, IL-6 and IL-8. iii This study extend the knowledge of the actions of the venom of the scorpion Tityus serrulatus on bronchial epithelial and endothelial cells thus demonstrating changes in pulmonary physiology that may contribute to a better treatment strategy of scorpion envenomation. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-13 |
dc.date.accessioned.fl_str_mv |
2015-07-27T14:08:11Z |
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info:eu-repo/semantics/publishedVersion |
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dc.identifier.citation.fl_str_mv |
Rigoni, Vera Lucia Silva. Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd). 2013. 53 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo . |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1149 |
identifier_str_mv |
Rigoni, Vera Lucia Silva. Efeito do veneno do escorpi??o Tityus serrulatus em c??lulas epiteliais br??nquicas humanas (BEAS) e c??lulas endoteliais (tEnd). 2013. 53 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo . |
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http://bibliotecatede.uninove.br/handle/tede/1149 |
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