Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares.
Ano de defesa: | 2009 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
|
Departamento: |
Sa??de
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/tede/handle/tede/814 |
Resumo: | Ultrasound has been one of the most widely and frequently used electro-therapeutic modalities for over 60 years. However, its effectiveness in treating pain, musculoskeletal disorders and soft tissue lesions remains questionable and its therapeutic potential is not fully established. In order to contribute to knowledge in this field, the objectives of the present study were to carry out a systematic review on this subject and perform an in vitro study for the assessment of the effect of therapeutic pulsed ultrasound on the proliferation and gelatinolytic activity of skeletal muscle cells (C2C12). Ultrasound was tested at frequencies of 1 and 3 MHz, powers of 0.2 and 0.5 W/cm2 and pulsed mode at 20% with application periods of 2 and 5 min. Cell proliferation was measured indirectly 72 hours after irradiation by assessing mitochondrial activity using the MTT method. Gelatinolytic activity in the supernatant of the cell cultures was assessed by zymography, complimented with densitometry analysis of bands that furnished gelatin hydrolysis. The control group was made up of cell cultures not submitted to ultrasound. The data were treated statistically with a significance level of 0.5% (p ??? 0.05 - ANOVA-Dunnet). The systematic review revealed considerable controversy among the results and parameters employed, which renders definitive conclusions regarding the potential of ultrasound on muscle tissue impossible. The experiments revealed no significant differences in cell proliferation between ultrasound-treated myoblasts and control cultures after 72 hours of incubation. The same was true in the densitometry analysis of the gels. Thus, there is a need for further investigations in order to gain a better understanding of the biochemical, cellular and organic reactions triggered by ultrasound as well as for the establishment standards of frequency, intensity, exposure time and application mode of this therapeutic resource. |
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Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Renno, Ana Claudia Munizhttp://lattes.cnpq.br/4106611304688552Silva, Carlos AlbertoCPF:00000000000CPF:29917734805http://lattes.cnpq.br/4830673791201102Ribeiro, Rafael2015-04-22T17:20:22Z2010-04-142009-12-16RIBEIRO, Rafael. Effect of the therapeutical ultrasound beaten in the proliferation and gelatinol??tica activity of muscular cells.. 2009. 59 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2009.http://bibliotecatede.uninove.br/tede/handle/tede/814Ultrasound has been one of the most widely and frequently used electro-therapeutic modalities for over 60 years. However, its effectiveness in treating pain, musculoskeletal disorders and soft tissue lesions remains questionable and its therapeutic potential is not fully established. In order to contribute to knowledge in this field, the objectives of the present study were to carry out a systematic review on this subject and perform an in vitro study for the assessment of the effect of therapeutic pulsed ultrasound on the proliferation and gelatinolytic activity of skeletal muscle cells (C2C12). Ultrasound was tested at frequencies of 1 and 3 MHz, powers of 0.2 and 0.5 W/cm2 and pulsed mode at 20% with application periods of 2 and 5 min. Cell proliferation was measured indirectly 72 hours after irradiation by assessing mitochondrial activity using the MTT method. Gelatinolytic activity in the supernatant of the cell cultures was assessed by zymography, complimented with densitometry analysis of bands that furnished gelatin hydrolysis. The control group was made up of cell cultures not submitted to ultrasound. The data were treated statistically with a significance level of 0.5% (p ??? 0.05 - ANOVA-Dunnet). The systematic review revealed considerable controversy among the results and parameters employed, which renders definitive conclusions regarding the potential of ultrasound on muscle tissue impossible. The experiments revealed no significant differences in cell proliferation between ultrasound-treated myoblasts and control cultures after 72 hours of incubation. The same was true in the densitometry analysis of the gels. Thus, there is a need for further investigations in order to gain a better understanding of the biochemical, cellular and organic reactions triggered by ultrasound as well as for the establishment standards of frequency, intensity, exposure time and application mode of this therapeutic resource.O ultra-som tem sido usado clinicamente por mais de 60 anos, sendo uma das modalidades eletro-terap??uticas mais largamente e freq??entemente empregadas. Mesmo assim, sua efetividade no tratamento da dor, das les??es m??sculo-esquel??ticas e das les??es em tecido mole permanece question??vel e seu potencial terap??utico ainda n??o est?? totalmente estabelecido. Buscando contribuir para o conhecimento neste campo, esse trabalho teve como objetivos principais fazer um levantamento bibliogr??fico por meio de uma revis??o sistem??tica da literatura e avaliar por meio de estudo in vitro o efeito do ultra-som terap??utico pulsado sobre a prolifera????o e atividade gelatinol??tica de c??lulas musculares esquel??ticas (C2C12). No estudo in vitro, o ultra-som foi testado nas freq????ncias de 1 e 3 MHz, intensidades de 0,2 e 0,5 W/cm2, no modo pulsado a 20% com per??odos de aplica????o de 2 e 5 minutos. A prolifera????o celular foi mensurada indiretamente 72 horas ap??s a irradia????o, por meio da avalia????o da atividade mitocondrial das c??lulas (m??todo MTT) e a atividade gelatinol??tica presente no sobrenadante das culturas celulares foi avaliada por zimografia (complementada pela an??lise das densitometrias das bandas que proporcionaram hidr??lise da gelatina). O grupo controle foi representado por culturas celulares n??o submetidas ao ultra-som e os dados obtidos foram tratados estatisticamente com n??vel de signific??ncia de 0,5% (p ??? 0,05 - ANOVA-Dunnet). Na revis??o sistem??tica observou-se grande controv??rsia entre resultados e par??metros, sendo imposs??vel estabelecer conclus??es definitivas sobre o potencial terap??utico do US em tecido muscular. Os experimentos mostraram que n??o houve diferen??a significante na prolifera????o celular entre os mioblastos tratados com ultra-som e as culturas controles ap??s 72 horas de incuba????o, e tamb??m na an??lise de densitometria dos g??is. Portanto, h?? necessidade de novas investiga????es para a compreens??o das rea????es bioqu??micas, celulares e org??nicas desencadeadas pelo US bem como para o estabelecimento de padr??es de freq????ncia, intensidade, tempo e modo de aplica????o deste recurso terap??utico.Made available in DSpace on 2015-04-22T17:20:22Z (GMT). No. of bitstreams: 1 B_Rafael Ribeiro.pdf: 979988 bytes, checksum: dc1bc057d4a378f8e475caa0099f4afb (MD5) Previous issue date: 2009-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??deUltra-somMioblastosC2C12Prolifera????oZimografia.UltrasoundMyoblastsC2C12ProliferationZymography.CIENCIAS DA SAUDEEfeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares.Effect of the therapeutical ultrasound beaten in the proliferation and gelatinol??tica activity of muscular cells.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALB_Rafael Ribeiro.pdfapplication/pdf979988http://localhost:8080/tede/bitstream/tede/814/1/B_Rafael+Ribeiro.pdfdc1bc057d4a378f8e475caa0099f4afbMD51tede/8142018-06-15 12:35:25.899oai:localhost:tede/814Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:35:25Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
dc.title.alternative.eng.fl_str_mv |
Effect of the therapeutical ultrasound beaten in the proliferation and gelatinol??tica activity of muscular cells. |
title |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
spellingShingle |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. Ribeiro, Rafael Ultra-som Mioblastos C2C12 Prolifera????o Zimografia. Ultrasound Myoblasts C2C12 Proliferation Zymography. CIENCIAS DA SAUDE |
title_short |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
title_full |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
title_fullStr |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
title_full_unstemmed |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
title_sort |
Efeito do ultra-som terap??utico pulsado na prolifera????o e atividade gelatinol??tica de c??lulas musculares. |
author |
Ribeiro, Rafael |
author_facet |
Ribeiro, Rafael |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee1.fl_str_mv |
Renno, Ana Claudia Muniz |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4106611304688552 |
dc.contributor.referee2.fl_str_mv |
Silva, Carlos Alberto |
dc.contributor.referee2ID.fl_str_mv |
CPF:00000000000 |
dc.contributor.authorID.fl_str_mv |
CPF:29917734805 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4830673791201102 |
dc.contributor.author.fl_str_mv |
Ribeiro, Rafael |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Renno, Ana Claudia Muniz Silva, Carlos Alberto |
dc.subject.por.fl_str_mv |
Ultra-som Mioblastos C2C12 Prolifera????o Zimografia. |
topic |
Ultra-som Mioblastos C2C12 Prolifera????o Zimografia. Ultrasound Myoblasts C2C12 Proliferation Zymography. CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
Ultrasound Myoblasts C2C12 Proliferation Zymography. |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Ultrasound has been one of the most widely and frequently used electro-therapeutic modalities for over 60 years. However, its effectiveness in treating pain, musculoskeletal disorders and soft tissue lesions remains questionable and its therapeutic potential is not fully established. In order to contribute to knowledge in this field, the objectives of the present study were to carry out a systematic review on this subject and perform an in vitro study for the assessment of the effect of therapeutic pulsed ultrasound on the proliferation and gelatinolytic activity of skeletal muscle cells (C2C12). Ultrasound was tested at frequencies of 1 and 3 MHz, powers of 0.2 and 0.5 W/cm2 and pulsed mode at 20% with application periods of 2 and 5 min. Cell proliferation was measured indirectly 72 hours after irradiation by assessing mitochondrial activity using the MTT method. Gelatinolytic activity in the supernatant of the cell cultures was assessed by zymography, complimented with densitometry analysis of bands that furnished gelatin hydrolysis. The control group was made up of cell cultures not submitted to ultrasound. The data were treated statistically with a significance level of 0.5% (p ??? 0.05 - ANOVA-Dunnet). The systematic review revealed considerable controversy among the results and parameters employed, which renders definitive conclusions regarding the potential of ultrasound on muscle tissue impossible. The experiments revealed no significant differences in cell proliferation between ultrasound-treated myoblasts and control cultures after 72 hours of incubation. The same was true in the densitometry analysis of the gels. Thus, there is a need for further investigations in order to gain a better understanding of the biochemical, cellular and organic reactions triggered by ultrasound as well as for the establishment standards of frequency, intensity, exposure time and application mode of this therapeutic resource. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-12-16 |
dc.date.available.fl_str_mv |
2010-04-14 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Rafael. Effect of the therapeutical ultrasound beaten in the proliferation and gelatinol??tica activity of muscular cells.. 2009. 59 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2009. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/814 |
identifier_str_mv |
RIBEIRO, Rafael. Effect of the therapeutical ultrasound beaten in the proliferation and gelatinol??tica activity of muscular cells.. 2009. 59 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2009. |
url |
http://bibliotecatede.uninove.br/tede/handle/tede/814 |
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por |
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UNINOVE |
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