Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório
Ano de defesa: | 2020 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde
|
Departamento: |
Saúde
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/2656 |
Resumo: | The phenotypic plasticity of leukocytes in the different phases of the inflammation and repair process, is the reason these cells are considered key players in their control. The effects of Photobiomodulation (PBM) on the treatment of tissue lesions have been widely described, but little is known about their role on leukocyte polarization. This project aimed to evaluate the optical absorbance spectrum of monocytes, neutrophils and human lymphocytes as well as the effects of PBM with amber LED with 590 nm on the gene and protein expression of mediators produced by these cells, which have been polarized to either the pro- or anti-inflammatory / regulatory phenotype. Peripheral human blood leukocytes were cultured in RPMI with 5% or 10% fetal bovine serum (to activate the pro- or anti-inflammatory phenotypes respectively), polarized with LPS or IL-4+LPS for 2 hours and irradiated with amber LED 590 nm (4J; 70 mW; 23.3 mW / cm2; 2.66 J / cm2), using one application after 2h or two applications after 2h and 6h. The cultures were collected to evaluate the optical absorbance spectrum (neutrophils after 12 hours and monocytes and lymphocytes after 24 hours) and for the analysis of mRNA expression of the cytokines IL-1β, IL-6, IL-10, IL-17, TNF-α, IFNγ and VEGF using qPCR (after 4 hours for neutrophils and 24 hours for monocytes and lymphocytes). The culture supernatants were used for protein evaluation via enzyme immunoassay (neutrophils: IL-1β, TNFα and IL-10; lymphocytes: IFNγ, IL-17 and IL-10 and monocytes: IL-1β, IL-6, TNFα and IL -10). At least 3 experiments (samples from 3 individuals) were carried out in duplicate. Non-irradiated and non-polarized cells served as controls. Monocytes and neutrophils showed peak absorption between 520-570 nm and lymphocytes peak absorption between 450-570 nm and 770-900 nm. Irradiation with amber LED, applied to cells polarized to the pro-inflammatory profile, generated a decrease in the protein synthesis of the pro-inflammatory cytokines IL-6 and TNFα in the monocytes, a decrease in the production synthesis of TNFα in neutrophils and, in the lymphocytes, an increase in the production of IL- 17. In leukocytes polarized to the anti-inflammatory / regulatory profile, irradiation with amber LED reduced the production of IL-10 in monocytes and lymphocytes. These results show that leukocytes present peak absorption in regions of the spectrum which remain mostly unexplored in PBM. In addition, they show that amber LED is capable of modulating the expression of cytokines which are important to the modulation of inflammation and repair processes; this may contribute to the optimization of inflammatory processes, as well as immunotherapy treatments. |
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Fernandes, Kristianne Porta Santoshttp://lattes.cnpq.br/6532810307204867Rodrigues, Maria Fernanda Setúbal Destrohttp://lattes.cnpq.br/7153553682954852Fernandes, Kristianne Santos Portahttp://lattes.cnpq.br/6532810307204867Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Silva, Daniela de Fátima Teixeira dahttp://lattes.cnpq.br/5912729434641689Renno, Ana Claudia Munizhttp://lattes.cnpq.br/4106611304688552Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161http://lattes.cnpq.br/6886059864273222Sousa, Kaline de Brito2021-11-04T14:46:21Z2020-03-17Sousa, Kaline de Brito. Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório. 2020. 111 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2656The phenotypic plasticity of leukocytes in the different phases of the inflammation and repair process, is the reason these cells are considered key players in their control. The effects of Photobiomodulation (PBM) on the treatment of tissue lesions have been widely described, but little is known about their role on leukocyte polarization. This project aimed to evaluate the optical absorbance spectrum of monocytes, neutrophils and human lymphocytes as well as the effects of PBM with amber LED with 590 nm on the gene and protein expression of mediators produced by these cells, which have been polarized to either the pro- or anti-inflammatory / regulatory phenotype. Peripheral human blood leukocytes were cultured in RPMI with 5% or 10% fetal bovine serum (to activate the pro- or anti-inflammatory phenotypes respectively), polarized with LPS or IL-4+LPS for 2 hours and irradiated with amber LED 590 nm (4J; 70 mW; 23.3 mW / cm2; 2.66 J / cm2), using one application after 2h or two applications after 2h and 6h. The cultures were collected to evaluate the optical absorbance spectrum (neutrophils after 12 hours and monocytes and lymphocytes after 24 hours) and for the analysis of mRNA expression of the cytokines IL-1β, IL-6, IL-10, IL-17, TNF-α, IFNγ and VEGF using qPCR (after 4 hours for neutrophils and 24 hours for monocytes and lymphocytes). The culture supernatants were used for protein evaluation via enzyme immunoassay (neutrophils: IL-1β, TNFα and IL-10; lymphocytes: IFNγ, IL-17 and IL-10 and monocytes: IL-1β, IL-6, TNFα and IL -10). At least 3 experiments (samples from 3 individuals) were carried out in duplicate. Non-irradiated and non-polarized cells served as controls. Monocytes and neutrophils showed peak absorption between 520-570 nm and lymphocytes peak absorption between 450-570 nm and 770-900 nm. Irradiation with amber LED, applied to cells polarized to the pro-inflammatory profile, generated a decrease in the protein synthesis of the pro-inflammatory cytokines IL-6 and TNFα in the monocytes, a decrease in the production synthesis of TNFα in neutrophils and, in the lymphocytes, an increase in the production of IL- 17. In leukocytes polarized to the anti-inflammatory / regulatory profile, irradiation with amber LED reduced the production of IL-10 in monocytes and lymphocytes. These results show that leukocytes present peak absorption in regions of the spectrum which remain mostly unexplored in PBM. In addition, they show that amber LED is capable of modulating the expression of cytokines which are important to the modulation of inflammation and repair processes; this may contribute to the optimization of inflammatory processes, as well as immunotherapy treatments.A plasticidade fenotípica dos leucócitos nas diferentes fases do processo de inflamação e reparo, faz com que essas células sejam consideradas peças chaves no seu controle. Os efeitos da fotobiomodulação (FBM) no tratamento de lesões teciduais tem sido bastante descrito, mas pouco se conhece a respeito do seu papel sobre a polarização dos leucócitos. Este projeto objetivou avaliar o espectro de absorbância óptica de monócitos, neutrófilos e linfócitos humanos e os efeitos da FBM com LED ambar de 590 nm sobre a expressão gênica e proteica de mediadores produzidos por essas células polarizadas para os fenótipos pró ou anti- inflamatório/regulador. Leucócitos de sangue humano periférico foram cultivados em RPMI com soro fetal bovino 5% ou 10% (para ativar os fenótipos pró ou anti- inflamatório respectivamente), polarizados com LPS ou IL-4+LPS por 2h e irradiados com LED ambar 590 nm (4J; 70 mW; 23,3 mW/cm2; 2,66 J/cm2), utilizando uma aplicação após 2h ou duas aplicações após 2h e 6h. As culturas foram coletadas para avaliação do espectro de absorbância óptica (neutrófilos após 12h e monócitos e linfócitos após 24h) e análise de expressão de mRNA das citocinas IL-1β, IL-6, IL- 10, IL-17, TNF-α, IFNγ e VEGF por meio de qPCR (após 4h para neutrófilos e 24h para monócitos e linfócitos). Os sobrenadantes das culturas foram usados para avaliação proteica por imunoensaio enzimático (neutrófilos: IL-1β, TNFα e IL-10; linfócitos: IFNγ, IL-17 e IL-10 e monócitos: IL-1β, IL-6, TNFα e IL-10). Foram realizados pelo menos 3 experimentos (3 indivíduos) em duplicata amostral. Células não irradiadas e não polarizadas serviram como controle. Monócitos e neutrófilos apresentaram pico de absorção entre 520-570 nm e os linfócitos pico de absorção entre 450-570 nm e 770-900 nm. A irradiação com LED ambar nas células polarizadas para o perfil pró-inflamatório gerou nos monócitos redução na síntese proteica das citocinas pró-inflamatórias IL-6 e TNFα, nos neutrófilos a redução da produção de TNFα e nos linfócitos aumento na produção de IL-17. Nos leucócitos polarizados para o perfil anti-inflamatório/regulador, a irradiação com LED ambar reduziu a produção de IL-10 em monócitos e linfócitos. Esses resultados evidenciam que os leucócitos apresentam pico de absorção em regiões do espectro ainda pouco exploradas na FBM e que o LED ambar é capaz de modular a expressão de citocinas importantes na modulação dos processos de inflamação e reparo podendo vir a contribuir para otimização dos processos inflamatórios, bem como nos tratamentos de imunoterapia.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2021-11-04T14:46:21Z No. of bitstreams: 1 Kaline de Brito Sousa.pdf: 3957296 bytes, checksum: f2f078e41b1ac6502b3876a60b66342f (MD5)Made available in DSpace on 2021-11-04T14:46:21Z (GMT). No. of bitstreams: 1 Kaline de Brito Sousa.pdf: 3957296 bytes, checksum: f2f078e41b1ac6502b3876a60b66342f (MD5) Previous issue date: 2020-03-17application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdemonócitoslinfócitosneutrófilospró-inflamatórioanti- inflamatórioabsorbâncialedterapiamonocyteslymphocytesneutrophilspro-inflammatoryanti- inflammatoryabsorbanceled therapyCIENCIAS DA SAUDEAvaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatórioEffect evaluation of the photobiomodulation with amber LED on polarized human leucocytes for pro or anti-inflammatory phenotypesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALKaline de Brito Sousa.pdfKaline de Brito Sousa.pdfapplication/pdf3957296http://localhost:8080/tede/bitstream/tede/2656/2/Kaline+de+Brito+Sousa.pdff2f078e41b1ac6502b3876a60b66342fMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2656/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/26562021-11-04 12:46:21.441oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2021-11-04T14:46:21Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
dc.title.alternative.eng.fl_str_mv |
Effect evaluation of the photobiomodulation with amber LED on polarized human leucocytes for pro or anti-inflammatory phenotypes |
title |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
spellingShingle |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório Sousa, Kaline de Brito monócitos linfócitos neutrófilos pró-inflamatório anti- inflamatório absorbância ledterapia monocytes lymphocytes neutrophils pro-inflammatory anti- inflammatory absorbance led therapy CIENCIAS DA SAUDE |
title_short |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
title_full |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
title_fullStr |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
title_full_unstemmed |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
title_sort |
Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório |
author |
Sousa, Kaline de Brito |
author_facet |
Sousa, Kaline de Brito |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6532810307204867 |
dc.contributor.advisor-co1.fl_str_mv |
Rodrigues, Maria Fernanda Setúbal Destro |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7153553682954852 |
dc.contributor.referee1.fl_str_mv |
Fernandes, Kristianne Santos Porta |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6532810307204867 |
dc.contributor.referee2.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee3.fl_str_mv |
Silva, Daniela de Fátima Teixeira da |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5912729434641689 |
dc.contributor.referee4.fl_str_mv |
Renno, Ana Claudia Muniz |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/4106611304688552 |
dc.contributor.referee5.fl_str_mv |
Silva Junior, Jose Antonio |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/1990427833073161 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6886059864273222 |
dc.contributor.author.fl_str_mv |
Sousa, Kaline de Brito |
contributor_str_mv |
Fernandes, Kristianne Porta Santos Rodrigues, Maria Fernanda Setúbal Destro Fernandes, Kristianne Santos Porta Ferrari, Raquel Agnelli Mesquita Silva, Daniela de Fátima Teixeira da Renno, Ana Claudia Muniz Silva Junior, Jose Antonio |
dc.subject.por.fl_str_mv |
monócitos linfócitos neutrófilos pró-inflamatório anti- inflamatório absorbância ledterapia |
topic |
monócitos linfócitos neutrófilos pró-inflamatório anti- inflamatório absorbância ledterapia monocytes lymphocytes neutrophils pro-inflammatory anti- inflammatory absorbance led therapy CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
monocytes lymphocytes neutrophils pro-inflammatory anti- inflammatory absorbance led therapy |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The phenotypic plasticity of leukocytes in the different phases of the inflammation and repair process, is the reason these cells are considered key players in their control. The effects of Photobiomodulation (PBM) on the treatment of tissue lesions have been widely described, but little is known about their role on leukocyte polarization. This project aimed to evaluate the optical absorbance spectrum of monocytes, neutrophils and human lymphocytes as well as the effects of PBM with amber LED with 590 nm on the gene and protein expression of mediators produced by these cells, which have been polarized to either the pro- or anti-inflammatory / regulatory phenotype. Peripheral human blood leukocytes were cultured in RPMI with 5% or 10% fetal bovine serum (to activate the pro- or anti-inflammatory phenotypes respectively), polarized with LPS or IL-4+LPS for 2 hours and irradiated with amber LED 590 nm (4J; 70 mW; 23.3 mW / cm2; 2.66 J / cm2), using one application after 2h or two applications after 2h and 6h. The cultures were collected to evaluate the optical absorbance spectrum (neutrophils after 12 hours and monocytes and lymphocytes after 24 hours) and for the analysis of mRNA expression of the cytokines IL-1β, IL-6, IL-10, IL-17, TNF-α, IFNγ and VEGF using qPCR (after 4 hours for neutrophils and 24 hours for monocytes and lymphocytes). The culture supernatants were used for protein evaluation via enzyme immunoassay (neutrophils: IL-1β, TNFα and IL-10; lymphocytes: IFNγ, IL-17 and IL-10 and monocytes: IL-1β, IL-6, TNFα and IL -10). At least 3 experiments (samples from 3 individuals) were carried out in duplicate. Non-irradiated and non-polarized cells served as controls. Monocytes and neutrophils showed peak absorption between 520-570 nm and lymphocytes peak absorption between 450-570 nm and 770-900 nm. Irradiation with amber LED, applied to cells polarized to the pro-inflammatory profile, generated a decrease in the protein synthesis of the pro-inflammatory cytokines IL-6 and TNFα in the monocytes, a decrease in the production synthesis of TNFα in neutrophils and, in the lymphocytes, an increase in the production of IL- 17. In leukocytes polarized to the anti-inflammatory / regulatory profile, irradiation with amber LED reduced the production of IL-10 in monocytes and lymphocytes. These results show that leukocytes present peak absorption in regions of the spectrum which remain mostly unexplored in PBM. In addition, they show that amber LED is capable of modulating the expression of cytokines which are important to the modulation of inflammation and repair processes; this may contribute to the optimization of inflammatory processes, as well as immunotherapy treatments. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-03-17 |
dc.date.accessioned.fl_str_mv |
2021-11-04T14:46:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Sousa, Kaline de Brito. Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório. 2020. 111 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2656 |
identifier_str_mv |
Sousa, Kaline de Brito. Avaliação do efeito da fotobiomodulação com LED ambar sobre leucócitos humanos polarizados para fenótipos pró ou anti-inflamatório. 2020. 111 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/2656 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Saúde |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
instname_str |
Universidade Nove de Julho (UNINOVE) |
instacron_str |
UNINOVE |
institution |
UNINOVE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/2656/2/Kaline+de+Brito+Sousa.pdf http://localhost:8080/tede/bitstream/tede/2656/1/license.txt |
bitstream.checksum.fl_str_mv |
f2f078e41b1ac6502b3876a60b66342f bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
_version_ |
1797250764475727872 |