Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
|
Departamento: |
Sa??de
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/1826 |
Resumo: | Tendinitis is a common disorder of the musculoskeletal system with multiple pathological manifestations. Although most tendons have the ability to spontaneously repair after injury, scar tissue is formed that can prevent the tendon to perform normal functions. Studies have been conducted using phototherapy in different health areas, using low-intensity lasers and LEDs. However, there are few studies with LEDs therapy. The aim of this study was to investigate the effect of phototherapy with Laser (830nm) and LED (945nm) in the repair process of the calcaneus tendonitis in rats. The inflammation was induced by controlled contusion in the medial region of the Achilles tendon of the animals. Was used 112 young male Wistar rats (240 ?? 20g), divided into 14 groups with two different experimental periods. Groups CONT (control group), TEND (tendinitis group) during periods 7 and 14 days, LEDT (LED therapy, from th to 7th day and from 1th to 14th day), and LEDT delay (LED therapy from 7th to 14th day). The therapy was initiated 12h after the tendinitis induction, with a 48h interval between irradiations (dose 6J/point). The control animals received simulation of treatment; the same experimental design was used for Laser treatment. All groups were euthanized on the 7th or 14th day after the induction. The tendons were dissected, extracted, and sent for analysis. Were performed biomechanical and histological analysis to evaluate neutrophils and collagen, were also quantitated the inflammatory mediators by real-time polymerase chain reaction (RT-PCR) and the IL-6 and TNF- ?? levels by ELISA. The results showed that treatments with low-intensity lasers in the parameters used and the times studied reduces migration of inflammatory cells and improves the quality of repair, but the LED even decreasing the inflammation was not effective of biomechanical properties of tendon tissue. |
id |
NOVE_f2b39cf1c0423e44c3b02b2e561dc995 |
---|---|
oai_identifier_str |
oai:localhost:tede/1826 |
network_acronym_str |
NOVE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
repository_id_str |
|
spelling |
Carvalho, Regiane Albertini deCarvalho, Regiane Albertini deSilveira Junior, LandulfoGuirro, Rinaldo Roberto de JesusMarcos, Rodrigo Labathttp://lattes.cnpq.br/1635884678698403Casalechi, Heliodora Le??o2018-06-21T18:42:55Z2015-06-24Casalechi, Heliodora Le??o. Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato. 2015. 74 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1826Tendinitis is a common disorder of the musculoskeletal system with multiple pathological manifestations. Although most tendons have the ability to spontaneously repair after injury, scar tissue is formed that can prevent the tendon to perform normal functions. Studies have been conducted using phototherapy in different health areas, using low-intensity lasers and LEDs. However, there are few studies with LEDs therapy. The aim of this study was to investigate the effect of phototherapy with Laser (830nm) and LED (945nm) in the repair process of the calcaneus tendonitis in rats. The inflammation was induced by controlled contusion in the medial region of the Achilles tendon of the animals. Was used 112 young male Wistar rats (240 ?? 20g), divided into 14 groups with two different experimental periods. Groups CONT (control group), TEND (tendinitis group) during periods 7 and 14 days, LEDT (LED therapy, from th to 7th day and from 1th to 14th day), and LEDT delay (LED therapy from 7th to 14th day). The therapy was initiated 12h after the tendinitis induction, with a 48h interval between irradiations (dose 6J/point). The control animals received simulation of treatment; the same experimental design was used for Laser treatment. All groups were euthanized on the 7th or 14th day after the induction. The tendons were dissected, extracted, and sent for analysis. Were performed biomechanical and histological analysis to evaluate neutrophils and collagen, were also quantitated the inflammatory mediators by real-time polymerase chain reaction (RT-PCR) and the IL-6 and TNF- ?? levels by ELISA. The results showed that treatments with low-intensity lasers in the parameters used and the times studied reduces migration of inflammatory cells and improves the quality of repair, but the LED even decreasing the inflammation was not effective of biomechanical properties of tendon tissue.A tendinite ?? uma desordem comum do sistema musculoesquel??tico. Embora os tend??es tenham habilidade de repara????o espont??nea ap??s ferimento, o tecido da cicatriza????o que ?? formado pode impedir o tend??o de executar as fun????es normais. Estudos v??m sendo realizados utilizando a fototerapia em diferentes ??reas da sa??de, empregando tanto laseres quanto LEDs de baixa intensidade. Contudo, existe um n??mero reduzido de estudos com a utiliza????o da terapia com LEDs. O objetivo deste estudo foi investigar os efeitos anti-inflamat??rios e as propriedades mec??nicas do tecido ap??s a a????o da fototerapia utilizando LASER (830nm) e LEDs (945nm), na regi??o do infravermelho, na tendinite induzida por trauma mec??nico em tend??o de Aquiles de ratos. Foram utilizados 112 ratos machos albinos da linhagem Wistar (240 ?? 20g), distribu??dos em 14 grupos e dois per??odos experimentais diferentes. Grupos CONT (grupo controle), os grupos TEND (grupos tendinite) nos per??odos de 7 e 14 dias, o LEDT (grupos terapia LED), do 1?? ao 7?? dia e do 1?? ao 14?? dia) e o grupo LEDT delay (terapia LED do 7?? ao 14?? dia). A terapia teve in??cio 12 horas ap??s a indu????o da tendinite, utilizando uma dose de 6J no ponto de aplica????o, com intervalos de 48 em 48 horas. A fototerapia foi aplicada transcutaneamente em um ??nico ponto sobre a regi??o lesionada. Os animais dos grupos controle foram submetidos ?? simula????o da aplica????o com o aparelho desligado, o mesmo desenho experimental foi utilizado feito para o tratamento Laser. As eutan??sias ocorreram ao 7?? e 14?? dia do experimento. Foi realizada an??lise biomec??nica, histopatol??gica para avalia????o de neutr??filos e col??geno, tamb??m foram quantificados os mediadores inflamat??rios por meio da RT-PCR e os n??veis de IL-6 e TNF- ?? foram avaliados pelo teste imunoenzim??tico (ELISA). Por meio dos resultados encontrados, ?? poss??vel inferir que os tratamentos com Laser de baixa intensidade, nos par??metros utilizados e nos tempos estudados melhora a qualidade do reparo do tecido tend??neo, a biomec??nica e reduz a migra????o de c??lulas inflamat??rias, j?? o LED de baixa intensidade apesar de diminuir a inflama????o n??o foi eficiente na manuten????o da biomec??nica do tend??o.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-21T18:42:55Z No. of bitstreams: 1 Heliodora Leao Casalechi.pdf: 2679810 bytes, checksum: f981677bdc05834caca5e26029e42566 (MD5)Made available in DSpace on 2018-06-21T18:42:55Z (GMT). No. of bitstreams: 1 Heliodora Leao Casalechi.pdf: 2679810 bytes, checksum: f981677bdc05834caca5e26029e42566 (MD5) Previous issue date: 2015-06-24application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??depropriedades mec??nicasmediadores inflamat??riostendiniteLaser de Baixa Intensidade (LBI)LED (Light Emitting Diode)mechanical propertiesinflammatory mediatorstendinitisLow Level LaserLED (Light Emitting Diode)CIENCIAS DA SAUDEModula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de ratoModulation of anti-inflammatory factors in a model of traumatic injury in rat Achilles tendoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALHeliodora Leao Casalechi.pdfHeliodora Leao Casalechi.pdfapplication/pdf2679810http://localhost:8080/tede/bitstream/tede/1826/2/Heliodora+Leao+Casalechi.pdff981677bdc05834caca5e26029e42566MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1826/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18262018-06-21 15:42:55.546oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-21T18:42:55Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
dc.title.alternative.eng.fl_str_mv |
Modulation of anti-inflammatory factors in a model of traumatic injury in rat Achilles tendon |
title |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
spellingShingle |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato Casalechi, Heliodora Le??o propriedades mec??nicas mediadores inflamat??rios tendinite Laser de Baixa Intensidade (LBI) LED (Light Emitting Diode) mechanical properties inflammatory mediators tendinitis Low Level Laser LED (Light Emitting Diode) CIENCIAS DA SAUDE |
title_short |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
title_full |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
title_fullStr |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
title_full_unstemmed |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
title_sort |
Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato |
author |
Casalechi, Heliodora Le??o |
author_facet |
Casalechi, Heliodora Le??o |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Carvalho, Regiane Albertini de |
dc.contributor.referee1.fl_str_mv |
Carvalho, Regiane Albertini de |
dc.contributor.referee2.fl_str_mv |
Silveira Junior, Landulfo |
dc.contributor.referee3.fl_str_mv |
Guirro, Rinaldo Roberto de Jesus |
dc.contributor.referee5.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1635884678698403 |
dc.contributor.author.fl_str_mv |
Casalechi, Heliodora Le??o |
contributor_str_mv |
Carvalho, Regiane Albertini de Carvalho, Regiane Albertini de Silveira Junior, Landulfo Guirro, Rinaldo Roberto de Jesus Marcos, Rodrigo Labat |
dc.subject.por.fl_str_mv |
propriedades mec??nicas mediadores inflamat??rios tendinite Laser de Baixa Intensidade (LBI) LED (Light Emitting Diode) |
topic |
propriedades mec??nicas mediadores inflamat??rios tendinite Laser de Baixa Intensidade (LBI) LED (Light Emitting Diode) mechanical properties inflammatory mediators tendinitis Low Level Laser LED (Light Emitting Diode) CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
mechanical properties inflammatory mediators tendinitis Low Level Laser LED (Light Emitting Diode) |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Tendinitis is a common disorder of the musculoskeletal system with multiple pathological manifestations. Although most tendons have the ability to spontaneously repair after injury, scar tissue is formed that can prevent the tendon to perform normal functions. Studies have been conducted using phototherapy in different health areas, using low-intensity lasers and LEDs. However, there are few studies with LEDs therapy. The aim of this study was to investigate the effect of phototherapy with Laser (830nm) and LED (945nm) in the repair process of the calcaneus tendonitis in rats. The inflammation was induced by controlled contusion in the medial region of the Achilles tendon of the animals. Was used 112 young male Wistar rats (240 ?? 20g), divided into 14 groups with two different experimental periods. Groups CONT (control group), TEND (tendinitis group) during periods 7 and 14 days, LEDT (LED therapy, from th to 7th day and from 1th to 14th day), and LEDT delay (LED therapy from 7th to 14th day). The therapy was initiated 12h after the tendinitis induction, with a 48h interval between irradiations (dose 6J/point). The control animals received simulation of treatment; the same experimental design was used for Laser treatment. All groups were euthanized on the 7th or 14th day after the induction. The tendons were dissected, extracted, and sent for analysis. Were performed biomechanical and histological analysis to evaluate neutrophils and collagen, were also quantitated the inflammatory mediators by real-time polymerase chain reaction (RT-PCR) and the IL-6 and TNF- ?? levels by ELISA. The results showed that treatments with low-intensity lasers in the parameters used and the times studied reduces migration of inflammatory cells and improves the quality of repair, but the LED even decreasing the inflammation was not effective of biomechanical properties of tendon tissue. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-06-24 |
dc.date.accessioned.fl_str_mv |
2018-06-21T18:42:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Casalechi, Heliodora Le??o. Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato. 2015. 74 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1826 |
identifier_str_mv |
Casalechi, Heliodora Le??o. Modula????o de fatores anti-inflamat??rios em modelo de les??o traum??tica em tend??o de Aquiles de rato. 2015. 74 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1826 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
instname_str |
Universidade Nove de Julho (UNINOVE) |
instacron_str |
UNINOVE |
institution |
UNINOVE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/1826/2/Heliodora+Leao+Casalechi.pdf http://localhost:8080/tede/bitstream/tede/1826/1/license.txt |
bitstream.checksum.fl_str_mv |
f981677bdc05834caca5e26029e42566 bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
_version_ |
1797250754894888960 |