Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos
Ano de defesa: | 2011 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Odontologia
|
Departamento: |
Faculdade de Odontologia
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/1174 |
Resumo: | The aim of this work was to compare clodronate and zoledronic acid effect on the repair of surgical wounds induced by tooth extraction and oral soft tissue lesion. Thirty-four rats (Rattus novergicus, Wistar) were allocated into 3 groups: (1) 12 animals treated with zoledronic acid; (2) 12 animals treated with clodronate; and (3) 10 animals that were given saline solution. Elapsed 90 days from the beginning of the treatment, the animals were subjected to tooth extractions and surgical-induced soft tissue injury in maxillae. At 180 days of drug administration, they were euthanized. After macroscopic evaluation, maxillae were processed and histological cuts were stained with hematoxylin and eosin (H&E). Immunohistochemical expression of RANKL, OPG, von Willebrand and caspase-3 was also evaluated. Non-vital bone, inflammatory infiltrate, microbial colonies, epithelial tissue, connective tissue and vital bone were quantified at the tissue wound sites. At the tooth extraction site, root fragments were also evaluated. The variables were quantified with Image Pro Plus software. Macroscopic analysis at the tooth extraction site showed that zoledronic acid group was associated with loss of mucosal integrity (chi-square, residual adjusted analysis, p<0.001), whereas at the soft tissue wound site, no group showed this association (chi-square, p=0.151). At the tooth extraction site, the zoledronic acid group showed greater proportion of non-vital bone and microbial colonies in comparison with the other groups. At the soft tissue wound site, the proportions of non-vital bone and microbial colonies were greater in the zoledronic acid and clodronate groups than in the control group. There was no significant difference for epithelial tissue, inflammatory infiltrate and root fragments between the groups (Kruskal-Wallis test complemented by its multiple comparisons test, p>0.05). Immunohistochemical expression of RANKL, OPG, von Willebrand and caspase-3 at tooth extraction and soft tissue wound sites did not differ significantly between the three groups analyzed (Kruskal-Wallis test, p>0.05). According to the results, (1) both bisphosphonates zoledronic acid and clodronate are capable of inducing osteonecrosis; (2) the immunohistochemical analysis suggests that the involvement of soft tissues as the initiator of osteonecrosis development is less probable than has been pointed out. |
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Cherubini, KarenCPF:55371400044http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795257U2CPF:82546150391http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137237Z8Vasconcelos, Ana Carolina Uchoa2015-04-14T13:30:14Z2012-06-052011-12-02VASCONCELOS, Ana Carolina Uchoa. Macro and microscopic evaluation of lesions induced by oral surgical procedures in rats under therapy with bisphosphonates. 2011. 127 f. Tese (Doutorado em Odontologia) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2011.http://tede2.pucrs.br/tede2/handle/tede/1174The aim of this work was to compare clodronate and zoledronic acid effect on the repair of surgical wounds induced by tooth extraction and oral soft tissue lesion. Thirty-four rats (Rattus novergicus, Wistar) were allocated into 3 groups: (1) 12 animals treated with zoledronic acid; (2) 12 animals treated with clodronate; and (3) 10 animals that were given saline solution. Elapsed 90 days from the beginning of the treatment, the animals were subjected to tooth extractions and surgical-induced soft tissue injury in maxillae. At 180 days of drug administration, they were euthanized. After macroscopic evaluation, maxillae were processed and histological cuts were stained with hematoxylin and eosin (H&E). Immunohistochemical expression of RANKL, OPG, von Willebrand and caspase-3 was also evaluated. Non-vital bone, inflammatory infiltrate, microbial colonies, epithelial tissue, connective tissue and vital bone were quantified at the tissue wound sites. At the tooth extraction site, root fragments were also evaluated. The variables were quantified with Image Pro Plus software. Macroscopic analysis at the tooth extraction site showed that zoledronic acid group was associated with loss of mucosal integrity (chi-square, residual adjusted analysis, p<0.001), whereas at the soft tissue wound site, no group showed this association (chi-square, p=0.151). At the tooth extraction site, the zoledronic acid group showed greater proportion of non-vital bone and microbial colonies in comparison with the other groups. At the soft tissue wound site, the proportions of non-vital bone and microbial colonies were greater in the zoledronic acid and clodronate groups than in the control group. There was no significant difference for epithelial tissue, inflammatory infiltrate and root fragments between the groups (Kruskal-Wallis test complemented by its multiple comparisons test, p>0.05). Immunohistochemical expression of RANKL, OPG, von Willebrand and caspase-3 at tooth extraction and soft tissue wound sites did not differ significantly between the three groups analyzed (Kruskal-Wallis test, p>0.05). According to the results, (1) both bisphosphonates zoledronic acid and clodronate are capable of inducing osteonecrosis; (2) the immunohistochemical analysis suggests that the involvement of soft tissues as the initiator of osteonecrosis development is less probable than has been pointed out.A presente pesquisa teve por objetivo comparar a influ?ncia dos bisfosfonatos ?cido zoledr?nico e clodronato no reparo de feridas cir?rgicas induzidas por meio de exodontia e les?o de tecido mole oral. Trinta e quatro ratos (Rattus novergicus, linhagem Wistar) foram distribu?dos em tr?s grupos: (1) 12 animais tratados com ?cido zoledr?nico; (2) 12 animais tratados com clodronato; e (3) 10 animais que receberam solu??o salina. Decorridos 90 dias do in?cio do tratamento, os animais foram submetidos a exodontias e procedimentos cir?rgicos em tecido mole, ambos na maxila. Aos 180 dias de administra??o dos f?rmacos, foi realizada a eutan?sia. Ap?s avalia??o macrosc?pica, as maxilas foram processadas, e os cortes histol?gicos corados por hematoxilina e eosina (H&E), bem como submetidos a processamento imunoistoqu?mico com os marcadores RANKL, OPG, von Willebrand e caspase-3. Nas l?minas coradas por H&E, foram quantificadas as vari?veis osso n?o-vital, osso vital, infiltrado inflamat?rio, col?nias microbianas, tecido epitelial e tecido conjuntivo, sendo que, no s?tio das exodontias, os restos radiculares tamb?m foram avaliados. A an?lise histol?gica foi realizada por meio do programa Image Pro Plus. Na avalia??o macrosc?pica, no s?tio das exodontias o grupo ?cido zoledr?nico exibiu associa??o com solu??o de continuidade da mucosa (qui-quadrado, an?lise de res?duos ajustados, p<0,001), enquanto na ?rea de les?o de tecido mole, nenhum grupo exibiu essa associa??o (qui-quadrado, p=0,151). No s?tio das exodontias, o grupo ?cido zoledr?nico exibiu propor??o significativamente maior de osso n?o-vital e col?nias microbianas do que os demais grupos. Na les?o de tecido mole, a propor??o de osso n?o-vital e col?nias microbianas foi significativamente maior nos grupos ?cido zoledr?nico e clodronato do que no controle. N?o houve diferen?a significativa das vari?veis tecido epitelial, infiltrado inflamat?rio e resto radicular entre os grupos (Kruskal-Wallis teste de compara??es m?ltiplas, p>0,05). A express?o imunoistoqu?mica de RANKL, OPG, von Willebrand e caspase-3 tamb?m n?o diferiu significativamente entre os grupos (Kruskal-Wallis, p>0,05). Os resultados permitem concluir que (1) ambos, ?cido zoledr?nico e clodronato, s?o capazes de induzir osteonecrose; (2) de acordo com a an?lise imunoistoqu?mica, ? pouco prov?vel que eventos intr?nsecos ? mucosa oral sejam os iniciadores da osteonecrose.Made available in DSpace on 2015-04-14T13:30:14Z (GMT). No. of bitstreams: 1 438871.pdf: 2788222 bytes, checksum: aa94bbb31ea254da3b082595c7658ecb (MD5) Previous issue date: 2011-12-02application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/8170/438871.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em OdontologiaPUCRSBRFaculdade de OdontologiaODONTOLOGIAESTOMATOLOGIA CL?NICAMUCOSA BUCAL - LES?ESOSSOS - NECROSEDIFOSFONATOSMARCADORES BIOL?GICOSRATOS - EXPERI?NCIASCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAvalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatosMacro and microscopic evaluation of lesions induced by oral surgical procedures in rats under therapy with bisphosphonatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-80965548187336651645006004673435736271820140info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL438871.pdf.jpg438871.pdf.jpgimage/jpeg2825http://tede2.pucrs.br/tede2/bitstream/tede/1174/3/438871.pdf.jpgef0656fb1145c678d6df4cee4e37d5e1MD53TEXT438871.pdf.txt438871.pdf.txttext/plain203450http://tede2.pucrs.br/tede2/bitstream/tede/1174/2/438871.pdf.txtdf8fd96e344142382bbea254cf01cffdMD52ORIGINAL438871.pdfapplication/pdf2788222http://tede2.pucrs.br/tede2/bitstream/tede/1174/1/438871.pdfaa94bbb31ea254da3b082595c7658ecbMD51tede/11742015-04-17 17:16:25.073oai:tede2.pucrs.br:tede/1174Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-04-17T20:16:25Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
dc.title.alternative.eng.fl_str_mv |
Macro and microscopic evaluation of lesions induced by oral surgical procedures in rats under therapy with bisphosphonates |
title |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
spellingShingle |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos Vasconcelos, Ana Carolina Uchoa ODONTOLOGIA ESTOMATOLOGIA CL?NICA MUCOSA BUCAL - LES?ES OSSOS - NECROSE DIFOSFONATOS MARCADORES BIOL?GICOS RATOS - EXPERI?NCIAS CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
title_full |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
title_fullStr |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
title_full_unstemmed |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
title_sort |
Avalia??o macro e microsc?pica de les?es orais induzidas por procedimentos cir?rgicos em ratos sob terapia com bisfosfonatos |
author |
Vasconcelos, Ana Carolina Uchoa |
author_facet |
Vasconcelos, Ana Carolina Uchoa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cherubini, Karen |
dc.contributor.advisor1ID.fl_str_mv |
CPF:55371400044 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795257U2 |
dc.contributor.authorID.fl_str_mv |
CPF:82546150391 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137237Z8 |
dc.contributor.author.fl_str_mv |
Vasconcelos, Ana Carolina Uchoa |
contributor_str_mv |
Cherubini, Karen |
dc.subject.por.fl_str_mv |
ODONTOLOGIA ESTOMATOLOGIA CL?NICA MUCOSA BUCAL - LES?ES OSSOS - NECROSE DIFOSFONATOS MARCADORES BIOL?GICOS RATOS - EXPERI?NCIAS |
topic |
ODONTOLOGIA ESTOMATOLOGIA CL?NICA MUCOSA BUCAL - LES?ES OSSOS - NECROSE DIFOSFONATOS MARCADORES BIOL?GICOS RATOS - EXPERI?NCIAS CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The aim of this work was to compare clodronate and zoledronic acid effect on the repair of surgical wounds induced by tooth extraction and oral soft tissue lesion. Thirty-four rats (Rattus novergicus, Wistar) were allocated into 3 groups: (1) 12 animals treated with zoledronic acid; (2) 12 animals treated with clodronate; and (3) 10 animals that were given saline solution. Elapsed 90 days from the beginning of the treatment, the animals were subjected to tooth extractions and surgical-induced soft tissue injury in maxillae. At 180 days of drug administration, they were euthanized. After macroscopic evaluation, maxillae were processed and histological cuts were stained with hematoxylin and eosin (H&E). Immunohistochemical expression of RANKL, OPG, von Willebrand and caspase-3 was also evaluated. Non-vital bone, inflammatory infiltrate, microbial colonies, epithelial tissue, connective tissue and vital bone were quantified at the tissue wound sites. At the tooth extraction site, root fragments were also evaluated. The variables were quantified with Image Pro Plus software. Macroscopic analysis at the tooth extraction site showed that zoledronic acid group was associated with loss of mucosal integrity (chi-square, residual adjusted analysis, p<0.001), whereas at the soft tissue wound site, no group showed this association (chi-square, p=0.151). At the tooth extraction site, the zoledronic acid group showed greater proportion of non-vital bone and microbial colonies in comparison with the other groups. At the soft tissue wound site, the proportions of non-vital bone and microbial colonies were greater in the zoledronic acid and clodronate groups than in the control group. There was no significant difference for epithelial tissue, inflammatory infiltrate and root fragments between the groups (Kruskal-Wallis test complemented by its multiple comparisons test, p>0.05). Immunohistochemical expression of RANKL, OPG, von Willebrand and caspase-3 at tooth extraction and soft tissue wound sites did not differ significantly between the three groups analyzed (Kruskal-Wallis test, p>0.05). According to the results, (1) both bisphosphonates zoledronic acid and clodronate are capable of inducing osteonecrosis; (2) the immunohistochemical analysis suggests that the involvement of soft tissues as the initiator of osteonecrosis development is less probable than has been pointed out. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-12-02 |
dc.date.available.fl_str_mv |
2012-06-05 |
dc.date.accessioned.fl_str_mv |
2015-04-14T13:30:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VASCONCELOS, Ana Carolina Uchoa. Macro and microscopic evaluation of lesions induced by oral surgical procedures in rats under therapy with bisphosphonates. 2011. 127 f. Tese (Doutorado em Odontologia) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2011. |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/1174 |
identifier_str_mv |
VASCONCELOS, Ana Carolina Uchoa. Macro and microscopic evaluation of lesions induced by oral surgical procedures in rats under therapy with bisphosphonates. 2011. 127 f. Tese (Doutorado em Odontologia) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2011. |
url |
http://tede2.pucrs.br/tede2/handle/tede/1174 |
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por |
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por |
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500 600 |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Odontologia |
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PUCRS |
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BR |
dc.publisher.department.fl_str_mv |
Faculdade de Odontologia |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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