Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Biologia Celular e Molecular
|
Departamento: |
Faculdade de Bioci?ncias
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/5454 |
Resumo: | Sepsis, a medical condition that affects 18 million people per year worldwide, is characterized by a generalized inflammatory state caused by infection. The widespread activation of coagulation pathways and inflammation progresses to multiple organ failure, the collapse of the circulatory system (septic shock) and death. Despite decades of research and numerous clinical trials, little progress has been made in developing new treatments and mortality rates are virtually the same in the last 20 to 30 years. As such, sepsis remains a difficult opponent for surgeons and their patients, so the search for new therapeutic alternatives becomes strictly essential. Recently stem cells have emerged as a promising therapy for a variety of diseases, including cardiovascular diseases, neurodegenerative disorders, peripheral vascular disease, renal disease, and several others. Its beneficial effects are due mainly to their ability to connect to injury and inflammation, to attenuate the inflammatory response, and accelerate tissue healing and neoangiogenesis due to noxious stimuli. Considering this therapeutic potential, this study aimed to evaluate whether these cells could lead to immune response back into balance, reducing the pathophysiology of sepsis and thereby increase the survival time in mice using an experimental model of sepsis. Our results demonstrated that treatment with mesenchymal stem cells was able to increase survival time of the animals that were tested. This effect is due to the ability of these cells to modulate the immune response providing a smaller reduction in tissue injury and apoptotic cells. These findings demonstrate that mesenchymal stem cells have therapeutic potential and can function as a possible future treatment for sepsis. |
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Oliveira, Jarbas Rodrigues deCPF:14090643015http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781448U1CPF:00918704022http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4236705Z3Pedrazza, Leonardo2015-04-14T14:51:22Z2013-04-252013-03-06PEDRAZZA, Leonardo. Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental. 2013. 87 f. Disserta??o (Mestrado em Biologia Celular e Molecular) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013.http://tede2.pucrs.br/tede2/handle/tede/5454Sepsis, a medical condition that affects 18 million people per year worldwide, is characterized by a generalized inflammatory state caused by infection. The widespread activation of coagulation pathways and inflammation progresses to multiple organ failure, the collapse of the circulatory system (septic shock) and death. Despite decades of research and numerous clinical trials, little progress has been made in developing new treatments and mortality rates are virtually the same in the last 20 to 30 years. As such, sepsis remains a difficult opponent for surgeons and their patients, so the search for new therapeutic alternatives becomes strictly essential. Recently stem cells have emerged as a promising therapy for a variety of diseases, including cardiovascular diseases, neurodegenerative disorders, peripheral vascular disease, renal disease, and several others. Its beneficial effects are due mainly to their ability to connect to injury and inflammation, to attenuate the inflammatory response, and accelerate tissue healing and neoangiogenesis due to noxious stimuli. Considering this therapeutic potential, this study aimed to evaluate whether these cells could lead to immune response back into balance, reducing the pathophysiology of sepsis and thereby increase the survival time in mice using an experimental model of sepsis. Our results demonstrated that treatment with mesenchymal stem cells was able to increase survival time of the animals that were tested. This effect is due to the ability of these cells to modulate the immune response providing a smaller reduction in tissue injury and apoptotic cells. These findings demonstrate that mesenchymal stem cells have therapeutic potential and can function as a possible future treatment for sepsis.Sepse, uma condi??o m?dica que afeta 18 milh?es de pessoas por ano no mundo, ? caracterizada por um estado inflamat?rio generalizado causado por uma infec??o. A ativa??o generalizada de vias de coagula??o e inflama??o evolui para disfun??o de m?ltiplos ?rg?os, o colapso do sistema circulat?rio (choque s?ptico) e morte. Apesar de d?cadas de pesquisas e numerosas experimenta??es cl?nicas, pouco progresso tem sido observado no desenvolvimento de novos tratamentos e as taxas de mortalidade s?o praticamente as mesmas nos ?ltimos 20 a 30 anos. Como tal, a sepse continua sendo um dif?cil advers?rio para cirurgi?es e seus pacientes, logo, a busca por novas alternativas terap?uticas torna-se estritamente essencial. Recentemente as c?lulas-tronco t?m emergido como uma terapia promissora para uma variedade de patologias, incluindo doen?as cardiovasculares, neurodegenerativas, doen?a vascular perif?rica, doen?a renal, e v?rias outras. Seus efeitos ben?ficos est?o relacionados principalmente ?s suas capacidades de se conectarem a les?es e inflama??es, para atenuar a resposta inflamat?ria e acelerar a cicatriza??o de tecidos e de neoangiog?nese devido a est?mulos nocivos. Considerando esse potencial terap?utico, o presente estudo teve por objetivo avaliar se essas c?lulas poderiam conduzir a resposta imune de volta ao equil?brio, atenuando a fisiopatologia da sepse e dessa forma aumentando o tempo de sobrevida em camundongos, utilizando um modelo de sepse experimental. Os resultados demonstraram que o tratamento com as c?lulas-tronco mesenquimais foi capaz de aumentar o tempo de sobrevida dos animais em estudo. Esse efeito observado deve-se ? capacidade destas c?lulas de modularem a resposta imune, proporcionando uma menor les?o tecidual e a diminui??o de c?lulas apopt?ticas. Essas descobertas demonstram que as c?lulas-tronco mesenquimais t?m potencial terap?utico e podem funcionar futuramente como um poss?vel tratamento para a sepse.Made available in DSpace on 2015-04-14T14:51:22Z (GMT). No. of bitstreams: 1 447381.pdf: 1128253 bytes, checksum: d5ebf0da0d18404190c4e67763e48457 (MD5) Previous issue date: 2013-03-06application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/16512/447381.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Biologia Celular e MolecularPUCRSBRFaculdade de Bioci?nciasBIOLOGIA CELULARSEPSEINFLAMA??OC?LULAS-TRONCO MESENQUIMAISCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAREfeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimentalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis819824693009663736060060036528317262667714info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL447381.pdf.jpg447381.pdf.jpgimage/jpeg3137http://tede2.pucrs.br/tede2/bitstream/tede/5454/3/447381.pdf.jpgc4e5767d2611171f4c35ee9aa77480c5MD53TEXT447381.pdf.txt447381.pdf.txttext/plain116890http://tede2.pucrs.br/tede2/bitstream/tede/5454/2/447381.pdf.txt6a683c90c6468f8dd3302c062deb61d6MD52ORIGINAL447381.pdfapplication/pdf1128253http://tede2.pucrs.br/tede2/bitstream/tede/5454/1/447381.pdfd5ebf0da0d18404190c4e67763e48457MD51tede/54542015-05-14 11:40:31.211oai:tede2.pucrs.br:tede/5454Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-05-14T14:40:31Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
title |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
spellingShingle |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental Pedrazza, Leonardo BIOLOGIA CELULAR SEPSE INFLAMA??O C?LULAS-TRONCO MESENQUIMAIS CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
title_full |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
title_fullStr |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
title_full_unstemmed |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
title_sort |
Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental |
author |
Pedrazza, Leonardo |
author_facet |
Pedrazza, Leonardo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Jarbas Rodrigues de |
dc.contributor.advisor1ID.fl_str_mv |
CPF:14090643015 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781448U1 |
dc.contributor.authorID.fl_str_mv |
CPF:00918704022 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4236705Z3 |
dc.contributor.author.fl_str_mv |
Pedrazza, Leonardo |
contributor_str_mv |
Oliveira, Jarbas Rodrigues de |
dc.subject.por.fl_str_mv |
BIOLOGIA CELULAR SEPSE INFLAMA??O C?LULAS-TRONCO MESENQUIMAIS |
topic |
BIOLOGIA CELULAR SEPSE INFLAMA??O C?LULAS-TRONCO MESENQUIMAIS CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
Sepsis, a medical condition that affects 18 million people per year worldwide, is characterized by a generalized inflammatory state caused by infection. The widespread activation of coagulation pathways and inflammation progresses to multiple organ failure, the collapse of the circulatory system (septic shock) and death. Despite decades of research and numerous clinical trials, little progress has been made in developing new treatments and mortality rates are virtually the same in the last 20 to 30 years. As such, sepsis remains a difficult opponent for surgeons and their patients, so the search for new therapeutic alternatives becomes strictly essential. Recently stem cells have emerged as a promising therapy for a variety of diseases, including cardiovascular diseases, neurodegenerative disorders, peripheral vascular disease, renal disease, and several others. Its beneficial effects are due mainly to their ability to connect to injury and inflammation, to attenuate the inflammatory response, and accelerate tissue healing and neoangiogenesis due to noxious stimuli. Considering this therapeutic potential, this study aimed to evaluate whether these cells could lead to immune response back into balance, reducing the pathophysiology of sepsis and thereby increase the survival time in mice using an experimental model of sepsis. Our results demonstrated that treatment with mesenchymal stem cells was able to increase survival time of the animals that were tested. This effect is due to the ability of these cells to modulate the immune response providing a smaller reduction in tissue injury and apoptotic cells. These findings demonstrate that mesenchymal stem cells have therapeutic potential and can function as a possible future treatment for sepsis. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-04-25 |
dc.date.issued.fl_str_mv |
2013-03-06 |
dc.date.accessioned.fl_str_mv |
2015-04-14T14:51:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PEDRAZZA, Leonardo. Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental. 2013. 87 f. Disserta??o (Mestrado em Biologia Celular e Molecular) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/5454 |
identifier_str_mv |
PEDRAZZA, Leonardo. Efeito terap?utico de c?lulas-tronco mesenquimais no tratamento da sepse experimental. 2013. 87 f. Disserta??o (Mestrado em Biologia Celular e Molecular) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013. |
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