Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares
Ano de defesa: | 2014 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de
|
Departamento: |
Escola de Medicina
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/8177 |
Resumo: | Objectives: To evaluate the expression of Hsp27 by immunohistochemistry in tissue samples of invasive ductal carcinoma in women with and without the presence of metastasis in sentinel axillary lymph node compared to samples from patients diagnosed with fibrocystic changes in breast tissue. In addition, to study the relationship between the rate of cell proliferation and apoptosis by evaluating the expression of Ki-67 protein and caspase-3 by immunohistochemistry. Material and Methods: A cross- sectional study was designed to select patients treated at Hospital S?o Lucas of PUCRS between September 2001 and October 2009. The patients were divided in three groups: 25 patients diagnosed with invasive ductal carcinoma of breast with axillary lymph node metastasis, 25 patients with invasive ductal carcinoma of breast without axillary lymph node metastasis and a control group composed by tissue samples of 25 patients diagnosed with fibrocystic change of the breast. The expression of Hsp27, Ki-67 and caspase 3 expression was evaluated by image analysis and the rate of proliferation (Ki-67) and apoptosis (caspase 3) was calculated for the three groups. Results: The present study showed an increased expression of Hsp27 in the group of invasive ductal carcinomas without metastasis as compared to the control group. There was no significant difference of expression of Hsp27 between the group of ductal carcinoma with axillary metastasis and the control group. The expression of Hsp27 was significantly higher in the group without axillary metastasis as compared to the group with metastasis in both the primary tumor (<0.001) and the respective lymph node tissue samples (=0.003). The expression of Ki-67 and the rate of proliferation (ki-67) and apoptosis (caspase 3) were significantly higher in the groups with invasive ductal carcinoma as compared to the control group. There was no difference in the expression of Ki-67 and the rate of proliferation and apoptosis between the two groups of invasive ductal carcinoma. There was no significant difference in the expression of caspase 3 among the three groups. Conclusion: The quantitative analysis of Hsp27 demonstrated increased expression of this protein in carcinomas without axillary metastases, with a significantly higher expression in the primary tumor and lymph node tissue samples of this group as compared to the group without axillary metastasis. Although the methodology employed and the number of cases evaluated in this work do not allow one to conclude that such behavior in metastatic ductal carcinomas of breast is constant, such finding warrants further investigation. |
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Silva, Vin?cius Duval dahttp://lattes.cnpq.br/5909263877910958http://lattes.cnpq.br/7631903589750164Birnfeld, Clarice Martins Feyh2018-06-29T12:38:48Z2014-10-31http://tede2.pucrs.br/tede2/handle/tede/8177Objectives: To evaluate the expression of Hsp27 by immunohistochemistry in tissue samples of invasive ductal carcinoma in women with and without the presence of metastasis in sentinel axillary lymph node compared to samples from patients diagnosed with fibrocystic changes in breast tissue. In addition, to study the relationship between the rate of cell proliferation and apoptosis by evaluating the expression of Ki-67 protein and caspase-3 by immunohistochemistry. Material and Methods: A cross- sectional study was designed to select patients treated at Hospital S?o Lucas of PUCRS between September 2001 and October 2009. The patients were divided in three groups: 25 patients diagnosed with invasive ductal carcinoma of breast with axillary lymph node metastasis, 25 patients with invasive ductal carcinoma of breast without axillary lymph node metastasis and a control group composed by tissue samples of 25 patients diagnosed with fibrocystic change of the breast. The expression of Hsp27, Ki-67 and caspase 3 expression was evaluated by image analysis and the rate of proliferation (Ki-67) and apoptosis (caspase 3) was calculated for the three groups. Results: The present study showed an increased expression of Hsp27 in the group of invasive ductal carcinomas without metastasis as compared to the control group. There was no significant difference of expression of Hsp27 between the group of ductal carcinoma with axillary metastasis and the control group. The expression of Hsp27 was significantly higher in the group without axillary metastasis as compared to the group with metastasis in both the primary tumor (<0.001) and the respective lymph node tissue samples (=0.003). The expression of Ki-67 and the rate of proliferation (ki-67) and apoptosis (caspase 3) were significantly higher in the groups with invasive ductal carcinoma as compared to the control group. There was no difference in the expression of Ki-67 and the rate of proliferation and apoptosis between the two groups of invasive ductal carcinoma. There was no significant difference in the expression of caspase 3 among the three groups. Conclusion: The quantitative analysis of Hsp27 demonstrated increased expression of this protein in carcinomas without axillary metastases, with a significantly higher expression in the primary tumor and lymph node tissue samples of this group as compared to the group without axillary metastasis. Although the methodology employed and the number of cases evaluated in this work do not allow one to conclude that such behavior in metastatic ductal carcinomas of breast is constant, such finding warrants further investigation.Objetivos: Avaliar a express?o de Hsp27 (HspB1) em amostras teciduais de carcinomas ductais invasores em mulheres com e sem presen?a de met?stase em linfonodo sentinela axilar e estudar a rela??o da taxa de prolifera??o celular e apoptose, atrav?s da avalia??o imunoistoqu?mica da express?o das prote?nas Hsp27, Ki-67 e caspase 3 em carcinomas ductais invasores de mulheres com e sem presen?a de met?stase linfonodal, tratadas em um hospital de ensino, comparado com amostras de pacientes diagnosticadas com altera??o fibroc?stica no tecido mam?rio. Material e M?todos: Foi realizado um estudo do tipo transversal, sendo selecionadas para este trabalho pacientes tratadas no Hospital S?o Lucas da PUCRS no per?odo entre setembro de 2001 e outubro de 2009 cujas bi?psias foram divididas em tr?s grupos: 25 pacientes com diagn?stico de carcinoma ductal invasor de mama com presen?a de met?stase linfonodal; e 25 pacientes com carcinoma ductal invasor de mama sem presen?a de met?stase linfonodal. Para constitui??o do grupo controle foi realizada a sele??o aleat?ria de 25 pacientes com diagn?stico de altera??o fibroc?stica da mama. Atrav?s da t?cnica de imunoistoqu?mica, tamb?m foram avaliadas as express?es de Hsp27, caspase 3 e Ki67 por an?lise de imagem digital e calculada a raz?o entre a taxa de prolifera??o (Ki-67) e apoptose (caspase 3) dos grupos estudados. Resultados: O presente estudo evidenciou a express?o aumentada da Hsp27 em carcinomas ductais invasores sem met?stase axilar, quando comparados ao grupo dos carcinomas ductais invasores com met?tase axilar e grupo controle. A express?o de Hsp 27 foi maior no grupo com carcinoma invasor sem met?stase axilar tanto no tecido da neoplasia prim?ria (p<0,001) quanto no respectivo tecido linfonodal comparado ao grupo com carcinoma com met?stase axilar (=0,003). Houve diferen?a na express?o da prote?na Ki-67 e raz?o entre prolifera??o celular e apoptose entre os grupos de pacientes com carcinoma ductal invasor com e sem met?stases quando comparados ao grupo controle, por?m n?o houve diferen?a significativa da express?o de Ki-67 e da raz?o entre prolifera??o e apoptose entre os grupos com carcinoma invasor com e sem met?stase, ou entre a express?o de caspase 3 nos tr?s grupos quando comparados entre si. Conclus?o: A an?lise quantitativa da Hsp27 demonstrou maior express?o da prote?na nos tecidos da neoplasia prim?ria e nos linfonodos em carcinomas sem met?stase em linfonodo sentinela axilar. Embora a metodologia empregada e o n?mero de casos estudados nesse trabalho n?o permitam concluir que esse comportamento na doen?a metast?tica seja um achado constante, esse resultado justifica a necessidade de avaliar esse achado em estudos futuros.Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2018-06-22T19:59:08Z No. of bitstreams: 1 CLARICE_MARTINS_FEYH_BIRNFELD.pdf: 3318155 bytes, checksum: aa5476b4fe73fb5e7e0b2678aef02e4b (MD5)Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-06-29T12:31:16Z (GMT) No. of bitstreams: 1 CLARICE_MARTINS_FEYH_BIRNFELD.pdf: 3318155 bytes, checksum: aa5476b4fe73fb5e7e0b2678aef02e4b (MD5)Made available in DSpace on 2018-06-29T12:38:48Z (GMT). No. of bitstreams: 1 CLARICE_MARTINS_FEYH_BIRNFELD.pdf: 3318155 bytes, checksum: aa5476b4fe73fb5e7e0b2678aef02e4b (MD5) Previous issue date: 2014-10-31application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/172675/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.jpghttps://tede2.pucrs.br/tede2/retrieve/188243/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Medicina e Ci?ncias da Sa?dePUCRSBrasilEscola de MedicinaHsp27Caspase3Ki67Carcinoma de MamaProlifera??o CelularApoptoseCIENCIAS DA SAUDE::MEDICINAExpress?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilaresinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTrabalho ser? publicado como artigo ou livro60 meses29/06/20237620745074616285884500500500-224747486637135387-969369452308786627info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSORIGINALDIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdfDIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdfapplication/pdf3318155https://tede2.pucrs.br/tede2/bitstream/tede/8177/5/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdfaa5476b4fe73fb5e7e0b2678aef02e4bMD55THUMBNAILDIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.jpgDIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.jpgimage/jpeg4094https://tede2.pucrs.br/tede2/bitstream/tede/8177/4/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.jpga64c0818567aac363af62ab45ce8c878MD54DIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.jpgDIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.jpgimage/jpeg5937https://tede2.pucrs.br/tede2/bitstream/tede/8177/7/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.jpg3e648f1e8b5a4e8749fd9b4a9558bd76MD57TEXTDIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.txtDIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.txttext/plain1077https://tede2.pucrs.br/tede2/bitstream/tede/8177/3/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_CONFIDENCIAL.pdf.txta9ebde07f50cb021ab070d961860d397MD53DIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.txtDIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.txttext/plain66622https://tede2.pucrs.br/tede2/bitstream/tede/8177/6/DIS_CLARICE_MARTINS_FEYH_BIRNFELD_COMPLETO.pdf.txt27e9d2a10e8288d722ef13bc911f43aaMD56LICENSElicense.txtlicense.txttext/plain; charset=utf-8610https://tede2.pucrs.br/tede2/bitstream/tede/8177/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/81772023-06-30 12:00:13.56oai:tede2.pucrs.br:tede/8177QXV0b3JpemHDp8OjbyBwYXJhIFB1YmxpY2HDp8OjbyBFbGV0csO0bmljYTogQ29tIGJhc2Ugbm8gZGlzcG9zdG8gbmEgTGVpIEZlZGVyYWwgbsK6OS42MTAsIGRlIDE5IGRlIGZldmVyZWlybyBkZSAxOTk4LCBvIGF1dG9yIEFVVE9SSVpBIGEgcHVibGljYcOnw6NvIGVsZXRyw7RuaWNhIGRhIHByZXNlbnRlIG9icmEgbm8gYWNlcnZvIGRhIEJpYmxpb3RlY2EgRGlnaXRhbCBkYSBQb250aWbDrWNpYSBVbml2ZXJzaWRhZGUgQ2F0w7NsaWNhIGRvIFJpbyBHcmFuZGUgZG8gU3VsLCBzZWRpYWRhIGEgQXYuIElwaXJhbmdhIDY2ODEsIFBvcnRvIEFsZWdyZSwgUmlvIEdyYW5kZSBkbyBTdWwsIGNvbSByZWdpc3RybyBkZSBDTlBKIDg4NjMwNDEzMDAwMi04MSBiZW0gY29tbyBlbSBvdXRyYXMgYmlibGlvdGVjYXMgZGlnaXRhaXMsIG5hY2lvbmFpcyBlIGludGVybmFjaW9uYWlzLCBjb25zw7NyY2lvcyBlIHJlZGVzIMOgcyBxdWFpcyBhIGJpYmxpb3RlY2EgZGEgUFVDUlMgcG9zc2EgYSB2aXIgcGFydGljaXBhciwgc2VtIMO0bnVzIGFsdXNpdm8gYW9zIGRpcmVpdG9zIGF1dG9yYWlzLCBhIHTDrXR1bG8gZGUgZGl2dWxnYcOnw6NvIGRhIHByb2R1w6fDo28gY2llbnTDrWZpY2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2023-06-30T15:00:13Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
title |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
spellingShingle |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares Birnfeld, Clarice Martins Feyh Hsp27 Caspase3 Ki67 Carcinoma de Mama Prolifera??o Celular Apoptose CIENCIAS DA SAUDE::MEDICINA |
title_short |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
title_full |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
title_fullStr |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
title_full_unstemmed |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
title_sort |
Express?o de HSP27 e raz?o entre os ?ndices de prolifera??o celular e apoptose em carcinoma de mama com e sem met?stases axilares |
author |
Birnfeld, Clarice Martins Feyh |
author_facet |
Birnfeld, Clarice Martins Feyh |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Vin?cius Duval da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5909263877910958 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7631903589750164 |
dc.contributor.author.fl_str_mv |
Birnfeld, Clarice Martins Feyh |
contributor_str_mv |
Silva, Vin?cius Duval da |
dc.subject.por.fl_str_mv |
Hsp27 Caspase3 Ki67 Carcinoma de Mama Prolifera??o Celular Apoptose |
topic |
Hsp27 Caspase3 Ki67 Carcinoma de Mama Prolifera??o Celular Apoptose CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Objectives: To evaluate the expression of Hsp27 by immunohistochemistry in tissue samples of invasive ductal carcinoma in women with and without the presence of metastasis in sentinel axillary lymph node compared to samples from patients diagnosed with fibrocystic changes in breast tissue. In addition, to study the relationship between the rate of cell proliferation and apoptosis by evaluating the expression of Ki-67 protein and caspase-3 by immunohistochemistry. Material and Methods: A cross- sectional study was designed to select patients treated at Hospital S?o Lucas of PUCRS between September 2001 and October 2009. The patients were divided in three groups: 25 patients diagnosed with invasive ductal carcinoma of breast with axillary lymph node metastasis, 25 patients with invasive ductal carcinoma of breast without axillary lymph node metastasis and a control group composed by tissue samples of 25 patients diagnosed with fibrocystic change of the breast. The expression of Hsp27, Ki-67 and caspase 3 expression was evaluated by image analysis and the rate of proliferation (Ki-67) and apoptosis (caspase 3) was calculated for the three groups. Results: The present study showed an increased expression of Hsp27 in the group of invasive ductal carcinomas without metastasis as compared to the control group. There was no significant difference of expression of Hsp27 between the group of ductal carcinoma with axillary metastasis and the control group. The expression of Hsp27 was significantly higher in the group without axillary metastasis as compared to the group with metastasis in both the primary tumor (<0.001) and the respective lymph node tissue samples (=0.003). The expression of Ki-67 and the rate of proliferation (ki-67) and apoptosis (caspase 3) were significantly higher in the groups with invasive ductal carcinoma as compared to the control group. There was no difference in the expression of Ki-67 and the rate of proliferation and apoptosis between the two groups of invasive ductal carcinoma. There was no significant difference in the expression of caspase 3 among the three groups. Conclusion: The quantitative analysis of Hsp27 demonstrated increased expression of this protein in carcinomas without axillary metastases, with a significantly higher expression in the primary tumor and lymph node tissue samples of this group as compared to the group without axillary metastasis. Although the methodology employed and the number of cases evaluated in this work do not allow one to conclude that such behavior in metastatic ductal carcinomas of breast is constant, such finding warrants further investigation. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-10-31 |
dc.date.accessioned.fl_str_mv |
2018-06-29T12:38:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://tede2.pucrs.br/tede2/handle/tede/8177 |
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http://tede2.pucrs.br/tede2/handle/tede/8177 |
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por |
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por |
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500 500 500 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de |
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PUCRS |
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Brasil |
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Escola de Medicina |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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