Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis
Ano de defesa: | 2022 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | eng |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ecologia e Recursos Naturais - PPGERN
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/15903 |
Resumo: | Microalgae require copper (Cu) in trace levels for their growth and metabolism. It is a vital component of certain metalloproteins, participates in the photosynthetic process, and catalyzes various redox reactions. Although this element has been widely studied concerning microalgae physiology, the effects of environmentally relevant levels have been less investigated. The aim of this study was to see the effect of environmental copper concentrations on some aspects of algae physiology, as growth rates, biomolecules (carbohydrates, lipids, proteins, and pigments) production, antioxidant response, and photosynthesis. For this, Ankistrodesmus flexuosus, Curvastrum pantanale, Monoraphidium sp., and Chlamydomonas chlorastera were the test organisms. They were kept under laboratory controlled conditions with Cu concentrations ranging from low (0.1 nM) to high (5480.0 nM) free Cu ions (Cu2+). Cultures lasted 96 h and all analyses were done in exponential growth phase. Cell densities and growth rates were unaffected in low Cu concentrations up to, 9.1 nM Cu2+ in C. chlorastera and 7.4 nM Cu2+ in the other species. In relation to pigments, β-carotene and lutein increased in C. chlorastera (1.2 mg g-1 β-carotene; 6.14 mg g-1 lutein) in 0.3–0.4 nM Cu2+. C. chlorastera had the highest, carbohydrates (> 50 pg cell-1) across all Cu concentrations tested, proteins content (270.2 pg cell-1; 0.3 nM Cu2+), and lipids (61.9% dw; 1.2 nM Cu2+). The activities of α, α-diphenyl-β-picrylhydrazyl (DPPH) radical, Glutathione S-transferase (GST), peroxidase (POD), superoxide dismutase (SOD) and malondialdehyde (MDA) content were not affected by low Cu exposure, but increased in high Cu. The microalgae effective quantum yields (ΔF⁄F_m') were more sensitive to Cu than their maximum quantum yields (F_v⁄F_m ). In Monoraphdium sp., Cu increase (3.4–7.4 nM Cu2+) increased photosynthesis, as recorded in the effective quantum yield (ΔF⁄F_m'), relative maximum electron transport rate (rETRm), saturation irradiance (Ek), and photochemical quenching (qP and qL). The non-photochemical quenching (NPQ) and PSII antenna size of Monoraphidium sp. increased in high Cu. Monoraphidium sp. also had the lowest photoinhibition (β) under high irradiance across all Cu exposures. The primary productivity of Monoraphidium sp. was unaltered in low Cu (1.7–21.4 nM Cu2+), but reduced by high Cu (589.0 nM Cu2+). Cu-elicited hormetic responses were seen in lipids content in C. chlorastera, and in photosynthesis in Monoraphidium sp. These findings show that depending on the species and concentration, Cu can stimulate biomolecules accumulation and increase photosynthesis in microalgae, without reducing growth. |
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Dauda, SuleimanLombardi, Ana Teresahttp://lattes.cnpq.br/6737850858443813http://lattes.cnpq.br/80634714057930122022-04-25T14:02:37Z2022-04-25T14:02:37Z2022-02-28DAUDA, Suleiman. Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis. 2022. Tese (Doutorado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15903.https://repositorio.ufscar.br/handle/ufscar/15903Microalgae require copper (Cu) in trace levels for their growth and metabolism. It is a vital component of certain metalloproteins, participates in the photosynthetic process, and catalyzes various redox reactions. Although this element has been widely studied concerning microalgae physiology, the effects of environmentally relevant levels have been less investigated. The aim of this study was to see the effect of environmental copper concentrations on some aspects of algae physiology, as growth rates, biomolecules (carbohydrates, lipids, proteins, and pigments) production, antioxidant response, and photosynthesis. For this, Ankistrodesmus flexuosus, Curvastrum pantanale, Monoraphidium sp., and Chlamydomonas chlorastera were the test organisms. They were kept under laboratory controlled conditions with Cu concentrations ranging from low (0.1 nM) to high (5480.0 nM) free Cu ions (Cu2+). Cultures lasted 96 h and all analyses were done in exponential growth phase. Cell densities and growth rates were unaffected in low Cu concentrations up to, 9.1 nM Cu2+ in C. chlorastera and 7.4 nM Cu2+ in the other species. In relation to pigments, β-carotene and lutein increased in C. chlorastera (1.2 mg g-1 β-carotene; 6.14 mg g-1 lutein) in 0.3–0.4 nM Cu2+. C. chlorastera had the highest, carbohydrates (> 50 pg cell-1) across all Cu concentrations tested, proteins content (270.2 pg cell-1; 0.3 nM Cu2+), and lipids (61.9% dw; 1.2 nM Cu2+). The activities of α, α-diphenyl-β-picrylhydrazyl (DPPH) radical, Glutathione S-transferase (GST), peroxidase (POD), superoxide dismutase (SOD) and malondialdehyde (MDA) content were not affected by low Cu exposure, but increased in high Cu. The microalgae effective quantum yields (ΔF⁄F_m') were more sensitive to Cu than their maximum quantum yields (F_v⁄F_m ). In Monoraphdium sp., Cu increase (3.4–7.4 nM Cu2+) increased photosynthesis, as recorded in the effective quantum yield (ΔF⁄F_m'), relative maximum electron transport rate (rETRm), saturation irradiance (Ek), and photochemical quenching (qP and qL). The non-photochemical quenching (NPQ) and PSII antenna size of Monoraphidium sp. increased in high Cu. Monoraphidium sp. also had the lowest photoinhibition (β) under high irradiance across all Cu exposures. The primary productivity of Monoraphidium sp. was unaltered in low Cu (1.7–21.4 nM Cu2+), but reduced by high Cu (589.0 nM Cu2+). Cu-elicited hormetic responses were seen in lipids content in C. chlorastera, and in photosynthesis in Monoraphidium sp. These findings show that depending on the species and concentration, Cu can stimulate biomolecules accumulation and increase photosynthesis in microalgae, without reducing growth.As microalgas requerem cobre (Cu) em níveis traço para seu crescimento e metabolismo. É um componente vital para certas metaloproteínas, participa do processo fotossintético e catalisa várias reações redox. Embora este elemento tenha sido amplamente estudado na fisiologia de microalgas, os efeitos de níveis ambientalmente relevantes foram ainda pouco investigados. O objetivo deste estudo foi verificar o efeito das concentrações ambientais de cobre em alguns aspectos da fisiologia de microalgas, como taxas de crescimento, produção de biomoléculas (carboidratos, lipídios, proteínas e pigmentos), resposta antioxidante e fotossíntese. Para isso, Ankistrodesmus flexuosus, Curvastrum pantanale, Chlamydomonas chlorastera e Monoraphidium sp. foram os organismos-teste. Eles foram mantidos sob condições controladas em laboratório com concentrações de cobre livre (Cu2+) variando de baixa (~0,1 nM) a alta (5480,0 nM). As culturas foram mantidas por 96 horas e todas as análises foram feitas na fase de crescimento exponencial. As densidades de celulares e as taxas de crescimento não foram afetadas em baixas concentrações de Cu até 9,1 nM Cu2+ em C. chlorastera, e 7,4 nM Cu2+ nas outras espécies. Em relação aos pigmentos, β-caroteno e luteína aumentaram em C. chlorastera (1,2 mg g-1 β-caroteno; 6,14 mg g-1 luteína) em 0,3–0,4 nM Cu2+. C. chlorastera apresentou os maiores, carboidratos (> 50 pg célula-1) em todas as concentrações de Cu testadas, teor de proteínas (270,2 pg célula-1; 0,3 nM Cu2+) e lipídios (61,9% dw; 1,2 nM Cu2+). As atividades do α, α-difenil-β-picrilhidrazil (DPPH), Glutationa S-transferase (GST), peroxidase (POD), superóxido dismutase (SOD), e teor de malondialdeído (MDA) não foram afetadas pela baixa exposição ao Cu, mas aumentou em alto Cu. Os rendimentos quânticos efetivos das microalgas (ΔF⁄F_m') foi mais sensíveis ao Cu do que o rendimentos quânticos máximos (F_v⁄F_m ). Em Monoraphdium sp., o aumento de Cu (3,4–7,4 nM Cu2+) aumentou a fotossíntese, conforme registrado no rendimento quântico efetivo (ΔF⁄F_m'), taxa de transporte de elétrons máxima relativa (rETRm), irradiância de saturação (Ek) e extinção fotoquímica (qP e qL). A extinção não fotoquímica (NPQ) e o tamanho da antena PSII de Monoraphidium sp. aumentou em alto Cu. Monoraphidium sp. também teve a menor fotoinibição (β) sob alta irradiância em todas as exposições de Cu. A produtividade primária de Monoraphidium sp. foi inalterado em baixo Cu (1,7-21,4 nM Cu2+), mas reduziu em alto Cu (589,0 nM Cu2+). Respostas horméticas induzidas por Cu foram observadas no conteúdo de lipídios em C. chlorastera e na fotossíntese em Monoraphidium sp. Esses resultados mostram que dependendo da espécie e concentração, o Cu pode estimular o acúmulo de biomoléculas e aumentar a fotossíntese em microalgas, sem reduzir o crescimento.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Processo nº 121853/2017-9, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)engUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Ecologia e Recursos Naturais - PPGERNUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessProdução de biomassaFisiologia de microalgasAntioxidantesFotossínteseBiomass productionMicroalgal physiologyAntioxidantsPhotosynthesisCIENCIAS BIOLOGICAS::ECOLOGIACIENCIAS BIOLOGICASPhysiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesisRespostas fisiológicas de microalgas chlorophyta sob concentrações ambientalmente relevantes de cobre: biomoléculas, estresse oxidativo e fotossínteseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTese_Suleiman_versao_final_28_04_2022.pdfTese_Suleiman_versao_final_28_04_2022.pdfTeseapplication/pdf3850001https://repositorio.ufscar.br/bitstream/ufscar/15903/9/Tese_Suleiman_versao_final_28_04_2022.pdf48352b0a1799fbee506d7b74e5d00c06MD59Formulário_Comprovante Versão Final Tese (1).pdfFormulário_Comprovante Versão Final Tese (1).pdfComprovante de submissão de versão finalapplication/pdf167457https://repositorio.ufscar.br/bitstream/ufscar/15903/3/Formul%c3%a1rio_Comprovante%20Vers%c3%a3o%20Final%20Tese%20%281%29.pdf183791607ee525a3b0434fd321cedf44MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15903/4/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD54TEXTTese_Suleiman_versao_final_28_04_2022.pdf.txtTese_Suleiman_versao_final_28_04_2022.pdf.txtExtracted texttext/plain182632https://repositorio.ufscar.br/bitstream/ufscar/15903/10/Tese_Suleiman_versao_final_28_04_2022.pdf.txt44da81be5c7e1a083fed0eeb7757a79dMD510Formulário_Comprovante Versão Final Tese (1).pdf.txtFormulário_Comprovante Versão Final Tese (1).pdf.txtExtracted texttext/plain1574https://repositorio.ufscar.br/bitstream/ufscar/15903/7/Formul%c3%a1rio_Comprovante%20Vers%c3%a3o%20Final%20Tese%20%281%29.pdf.txt4eb35d892908771acf021e5c4c907729MD57THUMBNAILTese_Suleiman_versao_final_28_04_2022.pdf.jpgTese_Suleiman_versao_final_28_04_2022.pdf.jpgIM Thumbnailimage/jpeg7429https://repositorio.ufscar.br/bitstream/ufscar/15903/11/Tese_Suleiman_versao_final_28_04_2022.pdf.jpgb4a12e6ca07b499c1f5996bf61588806MD511Formulário_Comprovante Versão Final Tese (1).pdf.jpgFormulário_Comprovante Versão Final Tese (1).pdf.jpgIM Thumbnailimage/jpeg14341https://repositorio.ufscar.br/bitstream/ufscar/15903/8/Formul%c3%a1rio_Comprovante%20Vers%c3%a3o%20Final%20Tese%20%281%29.pdf.jpg333a6e4a66a2dc730499efbc8f8af558MD58ufscar/159032022-05-11 13:15:52.322oai:repositorio.ufscar.br:ufscar/15903Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-05-25T13:03:24.279259Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
dc.title.alternative.por.fl_str_mv |
Respostas fisiológicas de microalgas chlorophyta sob concentrações ambientalmente relevantes de cobre: biomoléculas, estresse oxidativo e fotossíntese |
title |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
spellingShingle |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis Dauda, Suleiman Produção de biomassa Fisiologia de microalgas Antioxidantes Fotossíntese Biomass production Microalgal physiology Antioxidants Photosynthesis CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS |
title_short |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
title_full |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
title_fullStr |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
title_full_unstemmed |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
title_sort |
Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis |
author |
Dauda, Suleiman |
author_facet |
Dauda, Suleiman |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/8063471405793012 |
dc.contributor.author.fl_str_mv |
Dauda, Suleiman |
dc.contributor.advisor1.fl_str_mv |
Lombardi, Ana Teresa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6737850858443813 |
contributor_str_mv |
Lombardi, Ana Teresa |
dc.subject.por.fl_str_mv |
Produção de biomassa Fisiologia de microalgas Antioxidantes Fotossíntese Biomass production Microalgal physiology Antioxidants Photosynthesis |
topic |
Produção de biomassa Fisiologia de microalgas Antioxidantes Fotossíntese Biomass production Microalgal physiology Antioxidants Photosynthesis CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS |
description |
Microalgae require copper (Cu) in trace levels for their growth and metabolism. It is a vital component of certain metalloproteins, participates in the photosynthetic process, and catalyzes various redox reactions. Although this element has been widely studied concerning microalgae physiology, the effects of environmentally relevant levels have been less investigated. The aim of this study was to see the effect of environmental copper concentrations on some aspects of algae physiology, as growth rates, biomolecules (carbohydrates, lipids, proteins, and pigments) production, antioxidant response, and photosynthesis. For this, Ankistrodesmus flexuosus, Curvastrum pantanale, Monoraphidium sp., and Chlamydomonas chlorastera were the test organisms. They were kept under laboratory controlled conditions with Cu concentrations ranging from low (0.1 nM) to high (5480.0 nM) free Cu ions (Cu2+). Cultures lasted 96 h and all analyses were done in exponential growth phase. Cell densities and growth rates were unaffected in low Cu concentrations up to, 9.1 nM Cu2+ in C. chlorastera and 7.4 nM Cu2+ in the other species. In relation to pigments, β-carotene and lutein increased in C. chlorastera (1.2 mg g-1 β-carotene; 6.14 mg g-1 lutein) in 0.3–0.4 nM Cu2+. C. chlorastera had the highest, carbohydrates (> 50 pg cell-1) across all Cu concentrations tested, proteins content (270.2 pg cell-1; 0.3 nM Cu2+), and lipids (61.9% dw; 1.2 nM Cu2+). The activities of α, α-diphenyl-β-picrylhydrazyl (DPPH) radical, Glutathione S-transferase (GST), peroxidase (POD), superoxide dismutase (SOD) and malondialdehyde (MDA) content were not affected by low Cu exposure, but increased in high Cu. The microalgae effective quantum yields (ΔF⁄F_m') were more sensitive to Cu than their maximum quantum yields (F_v⁄F_m ). In Monoraphdium sp., Cu increase (3.4–7.4 nM Cu2+) increased photosynthesis, as recorded in the effective quantum yield (ΔF⁄F_m'), relative maximum electron transport rate (rETRm), saturation irradiance (Ek), and photochemical quenching (qP and qL). The non-photochemical quenching (NPQ) and PSII antenna size of Monoraphidium sp. increased in high Cu. Monoraphidium sp. also had the lowest photoinhibition (β) under high irradiance across all Cu exposures. The primary productivity of Monoraphidium sp. was unaltered in low Cu (1.7–21.4 nM Cu2+), but reduced by high Cu (589.0 nM Cu2+). Cu-elicited hormetic responses were seen in lipids content in C. chlorastera, and in photosynthesis in Monoraphidium sp. These findings show that depending on the species and concentration, Cu can stimulate biomolecules accumulation and increase photosynthesis in microalgae, without reducing growth. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-04-25T14:02:37Z |
dc.date.available.fl_str_mv |
2022-04-25T14:02:37Z |
dc.date.issued.fl_str_mv |
2022-02-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DAUDA, Suleiman. Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis. 2022. Tese (Doutorado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15903. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/15903 |
identifier_str_mv |
DAUDA, Suleiman. Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis. 2022. Tese (Doutorado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15903. |
url |
https://repositorio.ufscar.br/handle/ufscar/15903 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ecologia e Recursos Naturais - PPGERN |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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