Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino
Ano de defesa: | 2016 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEv
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/8613 |
Resumo: | Cervical cancer is the second largest cause of deaths from malignant cancer among women worldwide. Many studies show the importance of the HPV virus in the onset of cervical cancer, but because of the monoclonal nature of this cancer, it is suspected that HPV infection may not be considered the only causal factor responsible for the development of cervical carcinoma. Studies have indicated that EBV may have a role in cooperation with the HPV16 tumor development. Such cooperation could influence the tumor microenvironment by creating a favorable niche for its progression. Cancer cells secrete enzymes that degrade the extracellular matrix (ECM). Such enzymes such as matrix metalloproteinases (MMPs) facilitate cell movement in tissues and induce the activity of growth factors that promote angiogenesis. Among the group of MMPs, MMP-7 seems to have a crucial role in the development of carcinogenesis, since its action has been associated with the cleavage and release of adhesion molecules important. In this sense, this study was performed to establish the relationship between the HPV coinfection / EBV and expression of MMP-7. They were examined immunohistochemically histological slides of cervical lesions biopsies of 60 patients aged 35 to 65 years. The samples were divided into high grade lesion and low grade and evaluated semiquantitatively by scoring system ranging from 0 to +++. Histopathological indexes the degree of injury were established. It observed a statistically significant association between high-grade lesion, the expression of MMP-7 and the presence of the HPV virus, with p = 0.007 at the junction portion squamocolumnar and p = 0.023 in the portion of the ectocervix. |
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Silva, Naiara Soares Melo daAraújo, Heloísa Sobreiro Selistre dehttp://lattes.cnpq.br/4065824911933203Borra, Priscila Maria de Andradehttp://lattes.cnpq.br/3007992187979078http://lattes.cnpq.br/50899386824596132017-04-11T19:43:37Z2017-04-11T19:43:37Z2016-04-20SILVA, Naiara Soares Melo da. Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino. 2016. Dissertação (Mestrado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8613.https://repositorio.ufscar.br/handle/ufscar/8613Cervical cancer is the second largest cause of deaths from malignant cancer among women worldwide. Many studies show the importance of the HPV virus in the onset of cervical cancer, but because of the monoclonal nature of this cancer, it is suspected that HPV infection may not be considered the only causal factor responsible for the development of cervical carcinoma. Studies have indicated that EBV may have a role in cooperation with the HPV16 tumor development. Such cooperation could influence the tumor microenvironment by creating a favorable niche for its progression. Cancer cells secrete enzymes that degrade the extracellular matrix (ECM). Such enzymes such as matrix metalloproteinases (MMPs) facilitate cell movement in tissues and induce the activity of growth factors that promote angiogenesis. Among the group of MMPs, MMP-7 seems to have a crucial role in the development of carcinogenesis, since its action has been associated with the cleavage and release of adhesion molecules important. In this sense, this study was performed to establish the relationship between the HPV coinfection / EBV and expression of MMP-7. They were examined immunohistochemically histological slides of cervical lesions biopsies of 60 patients aged 35 to 65 years. The samples were divided into high grade lesion and low grade and evaluated semiquantitatively by scoring system ranging from 0 to +++. Histopathological indexes the degree of injury were established. It observed a statistically significant association between high-grade lesion, the expression of MMP-7 and the presence of the HPV virus, with p = 0.007 at the junction portion squamocolumnar and p = 0.023 in the portion of the ectocervix.O câncer cervical é responsável pela segunda maior causa de mortes por neoplasia malignas entre as mulheres no mundo. Muitos estudos comprovam a importância do vírus HPV no surgimento do câncer cervical, porém devido à natureza monoclonal desta neoplasia, suspeita-se que a infecção pelo HPV não possa ser considerada o único fator causal responsável pelo desenvolvimento do carcinoma de colo de útero. Estudos já apontaram que o EBV pode ter um papel de cooperação com o HPV16 no desenvolvimento tumoral. Tal cooperação poderia influenciar o microambiente tumoral criando um nicho favorável para a sua progressão. Células de câncer secretam enzimas que degradam a matriz extracelular (MEC). Tais enzimas, como as metaloproteinases de matriz (MMPs) facilitam a movimentação celular nos tecidos e induzem a atividade de fatores de crescimento que promovem a angiogênese. Dentre o grupo das MMPs, a MMP-7 parece ter papel crucial no desenvolvimento da carcinogênese, pois sua ação já foi associada com a clivagem e a liberação de importantes moléculas de adesão. Neste sentido, foi realizado este estudo para estabelecer a relação entre a co-infecção HPV/EBV e a expressão da MMP-7. Foram examinadas imunohistoquimicamente lâminas histológicas de biópsias de lesões de colo uterino de 60 pacientes com idades entre 35 e 65 anos. As amostras foram divididas em lesão de alto grau e de baixo grau e avaliados semiquantitativamente através de um sistema de escore variando de 0 à +++. Índices histopatológicos quanto ao grau das lesões foram estabelecidos. Foi observada uma associação estatisticamente significante entre a lesão de alto grau, a expressão da MMP-7 e a presença do vírus HPV, com p=0,007 na porção da junção escamocolunar e p=0,023 na porção da ectocérvice.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEvUFSCarCâncerColo uterinoMMP-7ImunohistoquímicaCancerCervicalImmunohistochemistryCIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAREstudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterinoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnlineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissNSMS.pdfDissNSMS.pdfapplication/pdf2315984https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/8613/1/DissNSMS.pdf1245c9af0e2575904b71a6d6569edf15MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/8613/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissNSMS.pdf.txtDissNSMS.pdf.txtExtracted texttext/plain119474https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/8613/3/DissNSMS.pdf.txta43f3f27b2521f7a96ea1f67c4c3a3ceMD53THUMBNAILDissNSMS.pdf.jpgDissNSMS.pdf.jpgIM Thumbnailimage/jpeg5628https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/8613/4/DissNSMS.pdf.jpg27726bbacda079ad3bf38b0bcc2497cfMD54ufscar/86132021-06-18 14:58:44.431oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-05-25T12:54:00.581967Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
title |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
spellingShingle |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino Silva, Naiara Soares Melo da Câncer Colo uterino MMP-7 Imunohistoquímica Cancer Cervical Immunohistochemistry CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
title_full |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
title_fullStr |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
title_full_unstemmed |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
title_sort |
Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino |
author |
Silva, Naiara Soares Melo da |
author_facet |
Silva, Naiara Soares Melo da |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/5089938682459613 |
dc.contributor.author.fl_str_mv |
Silva, Naiara Soares Melo da |
dc.contributor.advisor1.fl_str_mv |
Araújo, Heloísa Sobreiro Selistre de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4065824911933203 |
dc.contributor.advisor-co1.fl_str_mv |
Borra, Priscila Maria de Andrade |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3007992187979078 |
contributor_str_mv |
Araújo, Heloísa Sobreiro Selistre de Borra, Priscila Maria de Andrade |
dc.subject.por.fl_str_mv |
Câncer Colo uterino MMP-7 Imunohistoquímica |
topic |
Câncer Colo uterino MMP-7 Imunohistoquímica Cancer Cervical Immunohistochemistry CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.eng.fl_str_mv |
Cancer Cervical Immunohistochemistry |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
Cervical cancer is the second largest cause of deaths from malignant cancer among women worldwide. Many studies show the importance of the HPV virus in the onset of cervical cancer, but because of the monoclonal nature of this cancer, it is suspected that HPV infection may not be considered the only causal factor responsible for the development of cervical carcinoma. Studies have indicated that EBV may have a role in cooperation with the HPV16 tumor development. Such cooperation could influence the tumor microenvironment by creating a favorable niche for its progression. Cancer cells secrete enzymes that degrade the extracellular matrix (ECM). Such enzymes such as matrix metalloproteinases (MMPs) facilitate cell movement in tissues and induce the activity of growth factors that promote angiogenesis. Among the group of MMPs, MMP-7 seems to have a crucial role in the development of carcinogenesis, since its action has been associated with the cleavage and release of adhesion molecules important. In this sense, this study was performed to establish the relationship between the HPV coinfection / EBV and expression of MMP-7. They were examined immunohistochemically histological slides of cervical lesions biopsies of 60 patients aged 35 to 65 years. The samples were divided into high grade lesion and low grade and evaluated semiquantitatively by scoring system ranging from 0 to +++. Histopathological indexes the degree of injury were established. It observed a statistically significant association between high-grade lesion, the expression of MMP-7 and the presence of the HPV virus, with p = 0.007 at the junction portion squamocolumnar and p = 0.023 in the portion of the ectocervix. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-04-20 |
dc.date.accessioned.fl_str_mv |
2017-04-11T19:43:37Z |
dc.date.available.fl_str_mv |
2017-04-11T19:43:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Naiara Soares Melo da. Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino. 2016. Dissertação (Mestrado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8613. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/8613 |
identifier_str_mv |
SILVA, Naiara Soares Melo da. Estudo sobre a expressão da metaloproteinase de matriz 7 (MMP-7), a infecção pelos Vírus HPV e EBV e o grau de malignidade de lesões do colo uterino. 2016. Dissertação (Mestrado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8613. |
url |
https://repositorio.ufscar.br/handle/ufscar/8613 |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEv |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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