Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biotecnologia - PPGBiotec
|
Departamento: |
Não Informado pela instituição
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/6947 |
Resumo: | The elderly still go through physical changes, especially musculoskeletal disorders such as sarcopenia, changes in central and peripheral nervous system, blood hypoperfusion, regenerative changes contributing to atrophy and muscle weakness, undermining the activities of daily living (AVL). The regenerative process happens about the determination, proliferation and differentiation of satellite cells through activation of muscle-specific genetic program, which is regulated by specific transcription factors, known as myogenic regulatory factors (MRFs). But in the elderly because of changes in skeletal muscle-activation of MRFs are inefficient, hindering the process of regeneration. However, recent findings showed very promising results of low laser therapy (LLT) in muscle regeneration, but the effects of this therapy when associated with aging are still unknown. This project aims to evaluate the effects of (LLT), λ = 830nm, the tibial muscle of aged rats after cryolesioning. We used 56 male Wistar rats randomly divided into 4 groups (n = 7) of young rats from 3 months to 4 groups (n = 7) of aged rats, 10 months, divided into control groups (C), groups in which the right Tibialis anterior muscle (TA) was only irradiated (I), groups in which the AT was submitted to cryolesioning (CL) and groups where the TA muscle cryolesioning and was subjected to irradiation (LI). Treatment with the laser model of the DMC, Class 3B, energy of 0.87 J, was performed every 24 h for five consecutive days, with the first application 24 hours after induction of injury. On the sixth day after injury, with the animals anesthetized and the TA muscle was carefully dissected and removed, and then the animals were euthanaziated. We carried out histological analysis of the area of the lesion with toluidine blue, and counting of blood capillaries with hematoxylin-eosin. Through analysis by RT-PCR, it was possible to analyze the expression of MyoD and VEGF genes. The results showed that there was significant increase (p <0.05) of the expression of MyoD gene, VEGF gene and capillary blood count of more prominent in elderly victims and irradiated groups than in the young. Probably the LLT increased the maturation of satellite cells into myoblasts and miotubos, enhancing the regenerative process of aged rats irradiated. |
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Rodrigues, Natalia CamargoParizotto, Nivaldo Antoniohttp://lattes.cnpq.br/5527703735628408http://lattes.cnpq.br/86700983483975782016-08-17T18:39:30Z2009-08-252016-08-17T18:39:30Z2009-03-13RODRIGUES, Natalia Camargo. Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos. 2009. 55 f. Dissertação (Mestrado em Multidisciplinar) - Universidade Federal de São Carlos, São Carlos, 2009.https://repositorio.ufscar.br/handle/ufscar/6947The elderly still go through physical changes, especially musculoskeletal disorders such as sarcopenia, changes in central and peripheral nervous system, blood hypoperfusion, regenerative changes contributing to atrophy and muscle weakness, undermining the activities of daily living (AVL). The regenerative process happens about the determination, proliferation and differentiation of satellite cells through activation of muscle-specific genetic program, which is regulated by specific transcription factors, known as myogenic regulatory factors (MRFs). But in the elderly because of changes in skeletal muscle-activation of MRFs are inefficient, hindering the process of regeneration. However, recent findings showed very promising results of low laser therapy (LLT) in muscle regeneration, but the effects of this therapy when associated with aging are still unknown. This project aims to evaluate the effects of (LLT), λ = 830nm, the tibial muscle of aged rats after cryolesioning. We used 56 male Wistar rats randomly divided into 4 groups (n = 7) of young rats from 3 months to 4 groups (n = 7) of aged rats, 10 months, divided into control groups (C), groups in which the right Tibialis anterior muscle (TA) was only irradiated (I), groups in which the AT was submitted to cryolesioning (CL) and groups where the TA muscle cryolesioning and was subjected to irradiation (LI). Treatment with the laser model of the DMC, Class 3B, energy of 0.87 J, was performed every 24 h for five consecutive days, with the first application 24 hours after induction of injury. On the sixth day after injury, with the animals anesthetized and the TA muscle was carefully dissected and removed, and then the animals were euthanaziated. We carried out histological analysis of the area of the lesion with toluidine blue, and counting of blood capillaries with hematoxylin-eosin. Through analysis by RT-PCR, it was possible to analyze the expression of MyoD and VEGF genes. The results showed that there was significant increase (p <0.05) of the expression of MyoD gene, VEGF gene and capillary blood count of more prominent in elderly victims and irradiated groups than in the young. Probably the LLT increased the maturation of satellite cells into myoblasts and miotubos, enhancing the regenerative process of aged rats irradiated.Os idosos passam por continuas mudanças físicas, principalmente músculo-esqueléticas, como sarcopenia, alterações no sistema nervoso central e periférico, hipoperfusão sanguínea, alterações regenerativas contribuindo para atrofia e fraqueza muscular, prejudicando as atividades de vida diárias (AVDs). O processo regenerativo ocorre pela determinação, proliferação, diferenciação das células satélites através da ativação do programa genético músculo-específico, que é regulado por fatores de transcrição específicos, conhecidos como fatores regulatórios miogênicos (FRMs). Porém no idoso por causa das alterações músculo-esqueléticas a ativação dos FRMs são ineficientes, prejudicando o processo de regeneração. Entretanto, recentes achados mostraram resultados muito promissores da laser terapia de baixa intensidade (LTBI) na regeneração muscular, mas os efeitos desta terapia quando associado ao envelhecimento continuam desconhecidos. Este projeto tem por objetivo avaliar os efeitos da (LTBI), λ=830nm, no músculo tibial de ratos idosos após criolesão. Foram utilizados 56 ratos machos Wistar, divididos aleatoriamente em 4 grupos (n=7) de ratos jovens de 3 meses e 4 grupos (n=7) de ratos idosos de 10 meses; subdivididos em: grupos controle (C), grupos em que o músculo tibial anterior direito (TAD) foi apenas irradiado (I), grupos em que o TAD foi submetido à criolesão (CL) e grupos onde o TAD foi submetido à criolesão e a irradiação (LI). O tratamento com o laser modelo da DMC, classe 3B, energia de 0,87 J, foi realizado a cada 24 h, durante cinco dias consecutivos, com a primeira aplicação 24 horas após a indução da lesão. No sexto dia pós lesão, com os animais vivos e anestesiados, o músculo TAD foi cuidadosamente dissecado e retirado, e logo depois os animais foram eutanaziados. Realizou-se analises histológicas da área da lesão com Azul de Toluidina e contagem dos capilares sanguíneos com Hematoxilina-eosina. Por meio da análise por RT-PCR, foi possível analisar a expressão dos genes MyoD e VEGF. Os resultados mostraram que houve aumento significativo (p<0,05) da expressão gênica da MyoD, do VEGF e da contagem de capilares sanguíneos mais proeminentes nos grupos idosos lesados e irradiados do que no grupo jovem. Provavelmente a LTBI aumentou a maturação das células satélites em mioblastos e miotubos, melhorando o processo regenerativo dos ratos idosos irradiados.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Biotecnologia - PPGBiotecUFSCarBRBiotecnologiaLaserterapiaRegeneração muscularRatos idososMyoDVEGF (Fator de Crescimento de Endotélio Vascular)Terapia laser de baixa intensidade (LTBI)Low laser therapy (LLT)Muscle regenerationAged ratsOUTROSAção da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idososinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL2518.pdfapplication/pdf1232652https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/6947/1/2518.pdfe57f0134a52e7bb7bc66a121273dc146MD51TEXT2518.pdf.txt2518.pdf.txtExtracted texttext/plain72772https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/6947/2/2518.pdf.txt17775397c8eaea9cce3da0a1fddc5cb6MD52THUMBNAIL2518.pdf.jpg2518.pdf.jpgIM Thumbnailimage/jpeg6563https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/6947/3/2518.pdf.jpg10e7de410da4fccbaea88a0b9e51230aMD53ufscar/69472020-03-23 19:42:02.861oai:repositorio.ufscar.br:ufscar/6947Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-05-25T12:51:51.985476Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
title |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
spellingShingle |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos Rodrigues, Natalia Camargo Biotecnologia Laserterapia Regeneração muscular Ratos idosos MyoD VEGF (Fator de Crescimento de Endotélio Vascular) Terapia laser de baixa intensidade (LTBI) Low laser therapy (LLT) Muscle regeneration Aged rats OUTROS |
title_short |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
title_full |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
title_fullStr |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
title_full_unstemmed |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
title_sort |
Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos |
author |
Rodrigues, Natalia Camargo |
author_facet |
Rodrigues, Natalia Camargo |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/8670098348397578 |
dc.contributor.author.fl_str_mv |
Rodrigues, Natalia Camargo |
dc.contributor.advisor1.fl_str_mv |
Parizotto, Nivaldo Antonio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5527703735628408 |
contributor_str_mv |
Parizotto, Nivaldo Antonio |
dc.subject.por.fl_str_mv |
Biotecnologia Laserterapia Regeneração muscular Ratos idosos MyoD VEGF (Fator de Crescimento de Endotélio Vascular) Terapia laser de baixa intensidade (LTBI) |
topic |
Biotecnologia Laserterapia Regeneração muscular Ratos idosos MyoD VEGF (Fator de Crescimento de Endotélio Vascular) Terapia laser de baixa intensidade (LTBI) Low laser therapy (LLT) Muscle regeneration Aged rats OUTROS |
dc.subject.eng.fl_str_mv |
Low laser therapy (LLT) Muscle regeneration Aged rats |
dc.subject.cnpq.fl_str_mv |
OUTROS |
description |
The elderly still go through physical changes, especially musculoskeletal disorders such as sarcopenia, changes in central and peripheral nervous system, blood hypoperfusion, regenerative changes contributing to atrophy and muscle weakness, undermining the activities of daily living (AVL). The regenerative process happens about the determination, proliferation and differentiation of satellite cells through activation of muscle-specific genetic program, which is regulated by specific transcription factors, known as myogenic regulatory factors (MRFs). But in the elderly because of changes in skeletal muscle-activation of MRFs are inefficient, hindering the process of regeneration. However, recent findings showed very promising results of low laser therapy (LLT) in muscle regeneration, but the effects of this therapy when associated with aging are still unknown. This project aims to evaluate the effects of (LLT), λ = 830nm, the tibial muscle of aged rats after cryolesioning. We used 56 male Wistar rats randomly divided into 4 groups (n = 7) of young rats from 3 months to 4 groups (n = 7) of aged rats, 10 months, divided into control groups (C), groups in which the right Tibialis anterior muscle (TA) was only irradiated (I), groups in which the AT was submitted to cryolesioning (CL) and groups where the TA muscle cryolesioning and was subjected to irradiation (LI). Treatment with the laser model of the DMC, Class 3B, energy of 0.87 J, was performed every 24 h for five consecutive days, with the first application 24 hours after induction of injury. On the sixth day after injury, with the animals anesthetized and the TA muscle was carefully dissected and removed, and then the animals were euthanaziated. We carried out histological analysis of the area of the lesion with toluidine blue, and counting of blood capillaries with hematoxylin-eosin. Through analysis by RT-PCR, it was possible to analyze the expression of MyoD and VEGF genes. The results showed that there was significant increase (p <0.05) of the expression of MyoD gene, VEGF gene and capillary blood count of more prominent in elderly victims and irradiated groups than in the young. Probably the LLT increased the maturation of satellite cells into myoblasts and miotubos, enhancing the regenerative process of aged rats irradiated. |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-08-25 2016-08-17T18:39:30Z |
dc.date.issued.fl_str_mv |
2009-03-13 |
dc.date.accessioned.fl_str_mv |
2016-08-17T18:39:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Natalia Camargo. Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos. 2009. 55 f. Dissertação (Mestrado em Multidisciplinar) - Universidade Federal de São Carlos, São Carlos, 2009. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/6947 |
identifier_str_mv |
RODRIGUES, Natalia Camargo. Ação da laserterapia de baixa intensidade (830nm) na regeneração muscular de ratos idosos. 2009. 55 f. Dissertação (Mestrado em Multidisciplinar) - Universidade Federal de São Carlos, São Carlos, 2009. |
url |
https://repositorio.ufscar.br/handle/ufscar/6947 |
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openAccess |
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Universidade Federal de São Carlos |
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Programa de Pós-Graduação em Biotecnologia - PPGBiotec |
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UFSCar |
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BR |
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