Dinâmica e estrutura da comunidade procarionte da represa de Itupararanga - bacia do Rio Sorocaba - SP.

• Ano de defesa: 2013
• Autor(a) principal: Soares, Laís Américo
• Orientador(a): Santos, André Cordeiro Alves dos
• Banca de defesa: Duarte, Iolanda Cristina Silveira , Falco, Patrícia Bortoletto de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Carlos
• Programa de Pós-Graduação: Programa de Pos-graduação em Diversidade Biológica e Conservação
• País: BR
• Palavras-chave em Português: archaea; bacteria; coluna de água; PCR tempo real; PCR/DGGE; sedimento; archaea Rio Sorocaba (SP); bactérias Rio Sorocaba (SP)
• Palavras-chave em Inglês: archaea; bacteria; real time PCR; PCR/DGGE; sediment; water column.
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::ECOLOGIA
• Link de acesso: https://repositorio.ufscar.br/handle/ufscar/1526

This work aimed to quantifier the archaeal and bacterial communities by real time PCR, that shown the number of copies of 16S gene, rDNA into the water samples and to compare the archaeal and bacterial profiles on the PCR/DGGE technique as well as relate them with environmental variables in two points, dam and input on the Itupararanga reservoir, Sorocaba s basin. The bacterial density on the sediment (6,09 x108 e 2,56 x 109, dam and input, respectively) was more than water column (6,79 x 107 e 6,55 x 107, dam and input, respectively) this can be attributed to increase of the nutrients concentration from the surface to bottom. The bacterial (6,09 x 108 e 2,56 x 109 dam and input, respectively ) and archaeal (2,31 x 102 e 4,49 x 102 dam and input, respectively) quantities on the reservoir were more in the water column than in the sediment, which can be caused by the higher nutrients concentration in the top and the lees nutrients concentration in the bottom of Itupararanga reservoir. Since the canonical correspondence analysis has been possible, identify that the archaeal community has correlated with profundity and ammonia concentration suggesting ammonia oxidizing archaea s presence and abundance. The bacterial community quantity has correlated with physical chemical properties like pH and dissolved oxygen suggesting the environmental variables influence the group s abundance. Richness has correlated with nutrients distribution like orthophosphate concentration suggesting that the resources may limit the communities.