Sequenciamento e análise filogenética de amostras de Zika virus no Brasil
Ano de defesa: | 2018 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Católica de Brasília
|
Programa de Pós-Graduação: |
Programa Stricto Sensu em Ciências Genômicas e Biotecnologia
|
Departamento: |
Escola de Saúde e Medicina
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Resumo em Inglês: | Zika virus is an emergent flavivirus transmitted by mosquitos of Aedes genus, was first isolated in 1947 from Zika forest in Africa of the sentinel’s monkeys. The virus spread to other continents and was related with congenital and neurological syndromes in South America and became a public health issue. The genomic sequencing arouses as a crucial tool to obtain answers in real time about infections diseases outbreaks and has helped to understand the origin, phylogeny and trace Zika virus propagation. The main goals of this work are to obtain the complete Zika virus genome sequences that circulated in three different states of Brazil, DF, MT and TO, using the amplification technic multiplex PCR, the MinION portable platform of Oxford Nanopore. Besides that, we had the goal to generate the phylogenetic tree by maximum likelihood to better understand the origin, propagation and dissemination of the virus in these states, and consequentially, in Brazil. The sequences obtain in this present work shown high coverage, 94% to 100% was the coverage of the completeness of the genomes, that presented four distinct lineages, two different clades and related with Northeast samples of Brazil. Finally, our study provides new complete genome sequences of Zika virus in the state of Mato Grosso, demonstrate the relationship of the genome sequences of MT with sequences of Northeast of Brazil and suggests two possible entrances of Zika virus in the state of MT. |
Link de acesso: | https://bdtd.ucb.br:8443/jspui/handle/tede/2499 |
Resumo: | Zika virus is an emergent flavivirus transmitted by mosquitos of Aedes genus, was first isolated in 1947 from Zika forest in Africa of the sentinel’s monkeys. The virus spread to other continents and was related with congenital and neurological syndromes in South America and became a public health issue. The genomic sequencing arouses as a crucial tool to obtain answers in real time about infections diseases outbreaks and has helped to understand the origin, phylogeny and trace Zika virus propagation. The main goals of this work are to obtain the complete Zika virus genome sequences that circulated in three different states of Brazil, DF, MT and TO, using the amplification technic multiplex PCR, the MinION portable platform of Oxford Nanopore. Besides that, we had the goal to generate the phylogenetic tree by maximum likelihood to better understand the origin, propagation and dissemination of the virus in these states, and consequentially, in Brazil. The sequences obtain in this present work shown high coverage, 94% to 100% was the coverage of the completeness of the genomes, that presented four distinct lineages, two different clades and related with Northeast samples of Brazil. Finally, our study provides new complete genome sequences of Zika virus in the state of Mato Grosso, demonstrate the relationship of the genome sequences of MT with sequences of Northeast of Brazil and suggests two possible entrances of Zika virus in the state of MT. |
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Costa, Fabrício Falconihttp://lattes.cnpq.br/8964493624024135Melo, Fernando Lucashttp://lattes.cnpq.br/0092142990813893http://lattes.cnpq.br/7169104804413774Chanes, Carolina Rodrigues2018-11-22T11:49:14Z2018-08-24CHANES, Carolina Rodrigues. Sequenciamento e análise filogenética de amostras de Zika virus no Brasil. 2018. 59 f. Dissertação (Programa Stricto Sensu em Ciências Genômicas e Biotecnologia) - Universidade Católica de Brasília, Brasília, 2018.https://bdtd.ucb.br:8443/jspui/handle/tede/2499Zika virus is an emergent flavivirus transmitted by mosquitos of Aedes genus, was first isolated in 1947 from Zika forest in Africa of the sentinel’s monkeys. The virus spread to other continents and was related with congenital and neurological syndromes in South America and became a public health issue. The genomic sequencing arouses as a crucial tool to obtain answers in real time about infections diseases outbreaks and has helped to understand the origin, phylogeny and trace Zika virus propagation. The main goals of this work are to obtain the complete Zika virus genome sequences that circulated in three different states of Brazil, DF, MT and TO, using the amplification technic multiplex PCR, the MinION portable platform of Oxford Nanopore. Besides that, we had the goal to generate the phylogenetic tree by maximum likelihood to better understand the origin, propagation and dissemination of the virus in these states, and consequentially, in Brazil. The sequences obtain in this present work shown high coverage, 94% to 100% was the coverage of the completeness of the genomes, that presented four distinct lineages, two different clades and related with Northeast samples of Brazil. Finally, our study provides new complete genome sequences of Zika virus in the state of Mato Grosso, demonstrate the relationship of the genome sequences of MT with sequences of Northeast of Brazil and suggests two possible entrances of Zika virus in the state of MT.Zika virus é um flavivírus emergente transmitido por mosquitos do gênero Aedes, primeiramente isolado em 1947 de macacos sentinelas na floresta de Zika na África. O vírus se espalhou para outros continentes até ser relacionado com síndromes neurológicas e congênitas na América do Sul sendo taxado como um problema de saúde pública. O sequenciamento genômico emergiu como uma ferramenta crucial de respostas em tempo real a surtos de doenças infecciosas e tem ajudado a entender a filogenia, origem e delinear a propagação do Zika virus. Este trabalho tem como objetivos obter a sequência completa do Zika virus circulante em três diferentes estados do Brasil, DF, MT e TO, utilizando a técnica de amplificação por PCR multiplex, a plataforma portátil MinION da Oxford Nanopore. Além disto, tivemos como objetivo realizar a árvore filogenética por máxima verossimilhança a fim de compreender melhor a origem, propagação e disseminação do vírus nestes estados e, consequentemente, no Brasil. As sequências obtidas no presente trabalho apresentaram alta cobertura, foi possível cobrir entre 94% e 100% dos genomas que se apresentaram em quatro linhagens distintas e se associaram em dois clados diferentes relacionados as amostras do Nordeste do Brasil. Finalmente, nosso estudo fornece novas sequências completas do Zika virus no estado do Mato Grosso, demonstra a relação das sequencias do MT com as sequências do Nordeste do Brasil e sugere duas possíveis entradas do Zika virus no estado do MT.Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-11-22T11:48:56Z No. of bitstreams: 1 CarolinaRodriguesChanesDissertacao2018.pdf: 2882728 bytes, checksum: 9c589bc58e82d7391f2534b1161bc575 (MD5)Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-11-22T11:49:14Z (GMT) No. of bitstreams: 1 CarolinaRodriguesChanesDissertacao2018.pdf: 2882728 bytes, checksum: 9c589bc58e82d7391f2534b1161bc575 (MD5)Made available in DSpace on 2018-11-22T11:49:14Z (GMT). No. of bitstreams: 1 CarolinaRodriguesChanesDissertacao2018.pdf: 2882728 bytes, checksum: 9c589bc58e82d7391f2534b1161bc575 (MD5) Previous issue date: 2018-08-24application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/6076/CarolinaRodriguesChanesDissertacao2018.pdf.jpgporUniversidade Católica de BrasíliaPrograma Stricto Sensu em Ciências Genômicas e BiotecnologiaUCBBrasilEscola de Saúde e MedicinaZika virusFilogenéticaEpidemiologiaEpidemiologyPhylogeneticsCNPQ::CIENCIAS BIOLOGICAS::GENETICASequenciamento e análise filogenética de amostras de Zika virus no Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasíliainstacron:UCBTHUMBNAILCarolinaRodriguesChanesDissertacao2018.pdf.jpgCarolinaRodriguesChanesDissertacao2018.pdf.jpgimage/jpeg5366https://bdtd.ucb.br:8443/jspui/bitstream/tede/2499/4/CarolinaRodriguesChanesDissertacao2018.pdf.jpgd72c490b41f1f1fb5d3f68dd80011e8bMD54TEXTCarolinaRodriguesChanesDissertacao2018.pdf.txtCarolinaRodriguesChanesDissertacao2018.pdf.txttext/plain92396https://bdtd.ucb.br:8443/jspui/bitstream/tede/2499/3/CarolinaRodriguesChanesDissertacao2018.pdf.txt43f4464eb19fdc589d11c45b1c804d29MD53ORIGINALCarolinaRodriguesChanesDissertacao2018.pdfCarolinaRodriguesChanesDissertacao2018.pdfapplication/pdf2882728https://bdtd.ucb.br:8443/jspui/bitstream/tede/2499/2/CarolinaRodriguesChanesDissertacao2018.pdf9c589bc58e82d7391f2534b1161bc575MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81831https://bdtd.ucb.br:8443/jspui/bitstream/tede/2499/1/license.txtd7d5e5ec75089f122abe937645a56120MD51tede/2499oai:bdtd.ucb.br:tede/24992018-11-23 01:05:34.483Biblioteca Digital de Dissertações da Universidade Católica de Brasília - UCBsdi@ucb.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 |
dc.title.por.fl_str_mv |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
title |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
spellingShingle |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil Chanes, Carolina Rodrigues Zika virus Filogenética Epidemiologia Epidemiology Phylogenetics CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
title_full |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
title_fullStr |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
title_full_unstemmed |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
title_sort |
Sequenciamento e análise filogenética de amostras de Zika virus no Brasil |
author |
Chanes, Carolina Rodrigues |
author_facet |
Chanes, Carolina Rodrigues |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Costa, Fabrício Falconi |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8964493624024135 |
dc.contributor.advisor-co1.fl_str_mv |
Melo, Fernando Lucas |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0092142990813893 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7169104804413774 |
dc.contributor.author.fl_str_mv |
Chanes, Carolina Rodrigues |
contributor_str_mv |
Costa, Fabrício Falconi Melo, Fernando Lucas |
dc.subject.por.fl_str_mv |
Zika virus Filogenética Epidemiologia |
topic |
Zika virus Filogenética Epidemiologia Epidemiology Phylogenetics CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
dc.subject.eng.fl_str_mv |
Epidemiology Phylogenetics |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
dc.description.abstract.eng.fl_txt_mv |
Zika virus is an emergent flavivirus transmitted by mosquitos of Aedes genus, was first isolated in 1947 from Zika forest in Africa of the sentinel’s monkeys. The virus spread to other continents and was related with congenital and neurological syndromes in South America and became a public health issue. The genomic sequencing arouses as a crucial tool to obtain answers in real time about infections diseases outbreaks and has helped to understand the origin, phylogeny and trace Zika virus propagation. The main goals of this work are to obtain the complete Zika virus genome sequences that circulated in three different states of Brazil, DF, MT and TO, using the amplification technic multiplex PCR, the MinION portable platform of Oxford Nanopore. Besides that, we had the goal to generate the phylogenetic tree by maximum likelihood to better understand the origin, propagation and dissemination of the virus in these states, and consequentially, in Brazil. The sequences obtain in this present work shown high coverage, 94% to 100% was the coverage of the completeness of the genomes, that presented four distinct lineages, two different clades and related with Northeast samples of Brazil. Finally, our study provides new complete genome sequences of Zika virus in the state of Mato Grosso, demonstrate the relationship of the genome sequences of MT with sequences of Northeast of Brazil and suggests two possible entrances of Zika virus in the state of MT. |
dc.description.abstract.por.fl_txt_mv |
Zika virus é um flavivírus emergente transmitido por mosquitos do gênero Aedes, primeiramente isolado em 1947 de macacos sentinelas na floresta de Zika na África. O vírus se espalhou para outros continentes até ser relacionado com síndromes neurológicas e congênitas na América do Sul sendo taxado como um problema de saúde pública. O sequenciamento genômico emergiu como uma ferramenta crucial de respostas em tempo real a surtos de doenças infecciosas e tem ajudado a entender a filogenia, origem e delinear a propagação do Zika virus. Este trabalho tem como objetivos obter a sequência completa do Zika virus circulante em três diferentes estados do Brasil, DF, MT e TO, utilizando a técnica de amplificação por PCR multiplex, a plataforma portátil MinION da Oxford Nanopore. Além disto, tivemos como objetivo realizar a árvore filogenética por máxima verossimilhança a fim de compreender melhor a origem, propagação e disseminação do vírus nestes estados e, consequentemente, no Brasil. As sequências obtidas no presente trabalho apresentaram alta cobertura, foi possível cobrir entre 94% e 100% dos genomas que se apresentaram em quatro linhagens distintas e se associaram em dois clados diferentes relacionados as amostras do Nordeste do Brasil. Finalmente, nosso estudo fornece novas sequências completas do Zika virus no estado do Mato Grosso, demonstra a relação das sequencias do MT com as sequências do Nordeste do Brasil e sugere duas possíveis entradas do Zika virus no estado do MT. |
description |
Zika virus is an emergent flavivirus transmitted by mosquitos of Aedes genus, was first isolated in 1947 from Zika forest in Africa of the sentinel’s monkeys. The virus spread to other continents and was related with congenital and neurological syndromes in South America and became a public health issue. The genomic sequencing arouses as a crucial tool to obtain answers in real time about infections diseases outbreaks and has helped to understand the origin, phylogeny and trace Zika virus propagation. The main goals of this work are to obtain the complete Zika virus genome sequences that circulated in three different states of Brazil, DF, MT and TO, using the amplification technic multiplex PCR, the MinION portable platform of Oxford Nanopore. Besides that, we had the goal to generate the phylogenetic tree by maximum likelihood to better understand the origin, propagation and dissemination of the virus in these states, and consequentially, in Brazil. The sequences obtain in this present work shown high coverage, 94% to 100% was the coverage of the completeness of the genomes, that presented four distinct lineages, two different clades and related with Northeast samples of Brazil. Finally, our study provides new complete genome sequences of Zika virus in the state of Mato Grosso, demonstrate the relationship of the genome sequences of MT with sequences of Northeast of Brazil and suggests two possible entrances of Zika virus in the state of MT. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-22T11:49:14Z |
dc.date.issued.fl_str_mv |
2018-08-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.citation.fl_str_mv |
CHANES, Carolina Rodrigues. Sequenciamento e análise filogenética de amostras de Zika virus no Brasil. 2018. 59 f. Dissertação (Programa Stricto Sensu em Ciências Genômicas e Biotecnologia) - Universidade Católica de Brasília, Brasília, 2018. |
dc.identifier.uri.fl_str_mv |
https://bdtd.ucb.br:8443/jspui/handle/tede/2499 |
identifier_str_mv |
CHANES, Carolina Rodrigues. Sequenciamento e análise filogenética de amostras de Zika virus no Brasil. 2018. 59 f. Dissertação (Programa Stricto Sensu em Ciências Genômicas e Biotecnologia) - Universidade Católica de Brasília, Brasília, 2018. |
url |
https://bdtd.ucb.br:8443/jspui/handle/tede/2499 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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Universidade Católica de Brasília |
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Programa Stricto Sensu em Ciências Genômicas e Biotecnologia |
dc.publisher.initials.fl_str_mv |
UCB |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Saúde e Medicina |
publisher.none.fl_str_mv |
Universidade Católica de Brasília |
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