INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017
Ano de defesa: | 2023 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual do Centro-Oeste
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Florestais (Doutorado)
|
Departamento: |
Unicentro::Departamento de Ciências Florestais
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.unicentro.br:8080/jspui/handle/jspui/2186 |
Resumo: | Forest-based activity plays an important socioeconomic role in the country. Currently, Brazil is the leader in the rank of forest productivity, however, this high productivity has been affected by the constant attacks of pathogens that cause economic damage to these crops, an example is the myrtle rust caused by Austropuccinia psidii (G. Winter) Beenken, which has a wide geographic distribution and presents great genetic variability among its populations, attacking several species of the Myrtaceae family. In this sense, we sought to study population-genetic and evolutionary aspects of A. psidii populations. These data were about microsatellite loci for obtaining A. psidii isolates in about 148 populations of plants, from which they originated anddeposited in DRYAD. The model used in the estimates was of four subpopulations of A. psidii grouped based on seven hosts of the Myrtaceae family, being SPOP1 (Eucalyptus spp. + S. jambos), SPOP2 (P. guajava + P. guineense), SPOP3 (S. cumini) and SPOP4 (M. cauliflora and E. uniflora). These populations were formed from collections carried out by the authors of the deposits in the states of Bahia, Espírito Santo, Mato Grosso do Sul, Minas Gerais, Rio de Janeiro, Paraná, Santa Catarina, São Paulo, Rio Grande do Sul, in addition to Uruguay. Effective size and gene flow were estimated using the Bayesian inference method, based on the coalescence theory implemented in the MIGRATE-N software. In addition to descriptive statistics and graphs of population allelic patterns, paired estimates of FST and intra and intergroup differentiation by analysis of molecular variance (AMOVA) considering the genetic groups of four subpopulations and the seven hosts in a hierarchical manner. Gene flow (Nm) between population pairs was low (all < 1 unit), and ranged from 0.04 to 0.67. Estimates of effective population size (N and) were 119, 111, 189 and 1315 for SPOP1, SPOP2, SPOP3 and SPOP4 respectively. AMOVA showed that the percentage of variation was 39.43% between subpopulations, 16.29% between hosts within subpopulations and 44.28% within hosts. FST analogues were Φ CT = 0.394 (Among Subpopulations); Φ SC = 0.269 (Among Hosts within Subpopulation); Φ ST = 0.556 (Within the Hosts), in addition to high paired FST values that ranged between 0.355 and 0.560. The results obtained add knowledge about the understanding of A. psidii population genetics, proposing plausible scenarios in relation to the evolutionary and demographic past, as well as the processes that gave rise to the observed variability. Possibly the subpopulations of A. psidii will have different ecological behaviors and represent different invasive threats, being indicated studies that can measure the invasive risks presented by the different subpopulations. The work emphasizes the evident importance of genetic conservation in an adequate way in commercial species, aiming to guarantee the existence of resistant plant genotypes to be used in breeding programs, considering that resistance will be supplanted or reduced by the emergence of new races of A. psidii capable of adapting to the defenses present in the genotypes currently cultivated. |
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Garcia, Flávio Augusto de Oliveirahttp://lattes.cnpq.br/9237744351905960Tambarussi, Evandro Vagnerhttp://lattes.cnpq.br/3021997145272130Carolo Junior, Silviohttp://lattes.cnpq.br/2262985790178752084.267.289-36http://lattes.cnpq.br/7069800805962679PORTELA, RENAN MARCELO2024-03-20T13:18:18Z2023-02-17PORTELA, RENAN MARCELO. INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017. 2023. 70 f. Tese (Programa de Pós-Graduação em Ciências Florestais - Doutorado) - Universidade Estadual do Centro-Oeste, Irati - PR.http://tede.unicentro.br:8080/jspui/handle/jspui/2186Forest-based activity plays an important socioeconomic role in the country. Currently, Brazil is the leader in the rank of forest productivity, however, this high productivity has been affected by the constant attacks of pathogens that cause economic damage to these crops, an example is the myrtle rust caused by Austropuccinia psidii (G. Winter) Beenken, which has a wide geographic distribution and presents great genetic variability among its populations, attacking several species of the Myrtaceae family. In this sense, we sought to study population-genetic and evolutionary aspects of A. psidii populations. These data were about microsatellite loci for obtaining A. psidii isolates in about 148 populations of plants, from which they originated anddeposited in DRYAD. The model used in the estimates was of four subpopulations of A. psidii grouped based on seven hosts of the Myrtaceae family, being SPOP1 (Eucalyptus spp. + S. jambos), SPOP2 (P. guajava + P. guineense), SPOP3 (S. cumini) and SPOP4 (M. cauliflora and E. uniflora). These populations were formed from collections carried out by the authors of the deposits in the states of Bahia, Espírito Santo, Mato Grosso do Sul, Minas Gerais, Rio de Janeiro, Paraná, Santa Catarina, São Paulo, Rio Grande do Sul, in addition to Uruguay. Effective size and gene flow were estimated using the Bayesian inference method, based on the coalescence theory implemented in the MIGRATE-N software. In addition to descriptive statistics and graphs of population allelic patterns, paired estimates of FST and intra and intergroup differentiation by analysis of molecular variance (AMOVA) considering the genetic groups of four subpopulations and the seven hosts in a hierarchical manner. Gene flow (Nm) between population pairs was low (all < 1 unit), and ranged from 0.04 to 0.67. Estimates of effective population size (N and) were 119, 111, 189 and 1315 for SPOP1, SPOP2, SPOP3 and SPOP4 respectively. AMOVA showed that the percentage of variation was 39.43% between subpopulations, 16.29% between hosts within subpopulations and 44.28% within hosts. FST analogues were Φ CT = 0.394 (Among Subpopulations); Φ SC = 0.269 (Among Hosts within Subpopulation); Φ ST = 0.556 (Within the Hosts), in addition to high paired FST values that ranged between 0.355 and 0.560. The results obtained add knowledge about the understanding of A. psidii population genetics, proposing plausible scenarios in relation to the evolutionary and demographic past, as well as the processes that gave rise to the observed variability. Possibly the subpopulations of A. psidii will have different ecological behaviors and represent different invasive threats, being indicated studies that can measure the invasive risks presented by the different subpopulations. The work emphasizes the evident importance of genetic conservation in an adequate way in commercial species, aiming to guarantee the existence of resistant plant genotypes to be used in breeding programs, considering that resistance will be supplanted or reduced by the emergence of new races of A. psidii capable of adapting to the defenses present in the genotypes currently cultivated.A atividade de base florestal desempenha um importante papel socioeconômico no país. Atualmente, o Brasil é líder no ranque de produtividade em plantios florestais, entretanto, esta vem sendo afetada pelos ataques constantes de patógenos que ocasionam danos econômicos a esses cultivos. Um exemplo é a ferrugem em Eucalyptus spp., causada por Austropuccinia psidii (G. Winter) Beenken, que possui ampla distribuição geográfica e o patógeno apresenta grande variabilidade genética entre as suas populações, atacando também outras espécies da família Myrtaceae. Nesse sentido, buscou-se estudar aspectos genéticos-populacionais e evolucionários de populações de A. psidii. A base de dados foi obtida na plataforma DRYAD sob a licença CC0 1.0. Esses dados referem-se a loci microssatélites de isolados de A. psidii, obtidos a partir de isolados fúngicos advindos de amostra de 148 plantas, das quais originaram esses marcadores depositados no DRYAD. O modelo usado nas estimativas foi de quatro subpopulações de A. psidii agrupadas com base em sete hospedeiros da família Myrtaceae, sendo SPOP1 (Eucalyptus spp. +Syzygium jambos), SPOP2 (Psidium guajava + Psidium guineense), SPOP3 (Syzygium cumini) e SPOP4 (Myrciaria cauliflora e Eugenia uniflora). Essas subpopulações foram formadas com coletas feitas pelos autores do depósito dos dados nos estados da Bahia, Espírito Santo, Mato Grosso do Sul, Minas Gerais, Rio de Janeiro, Paraná, Santa Catarina, São Paulo, Rio Grande do Sul, além do Uruguai. Também estimou o tamanho efetivo e fluxo gênico entre as subpopulações, por meio do método de inferência bayesiana, baseado na teoria de coalescência implementada no software MIGRATE-N. Além de estatísticas descritivas e gráficos dos padrões alélicos populacionais, realizou estimativas pareadas de FST e diferenciação intra e intergrupos por análise de variância molecular (AMOVA) considerando os grupos genéticos de quatro subpopulações e os sete hospedeiros de maneira hierárquica. O fluxo gênico (Nm) entre os pares de populações foram baixos (todos < 1 unidade), e variou de 0,04 a 0,67. As estimativas do tamanho efetivo populacional (Ne) foram 119, 111, 189 e 1315 para SPOP1, SPOP2, SPOP3 e SPOP4, respectivamente. A AMOVA mostrou que a porcentagem da variação foi de 39,43% entre as subpopulações, 16,29% entre os hospedeiros dentro de subpopulação e 44,28% dentro dos hospedeiros. Os análogos de FST foram de ΦCT = 0,394 (Entre Subpopulações); ΦSC = 0,269 (Entre Hospedeiros dentro de Subpopulação); ΦST = 0,556 (Dentro dos Hospedeiros), além de altos valores pareados de FST que variaram entre 0,355 e 0,560. Os resultados obtidos agregam conhecimento acerca da compreensão da genética de populações de A. psidii, propondo cenários plausíveis em relação ao passado evolutivo e demográfico, bem como os processos que deram origem a variabilidade observadas. Possivelmente as subpopulações de A. psidii terão diferentes comportamentos ecológicos e representar ameaças invasivas distintas, sendo indicado estudos que possam mensurar os riscos invasivos apresentados pelas diferentes subpopulações. O trabalho ressalta a evidente importância da conservação genética de maneira adequada em espécies florestais comerciais, visando garantir a existência genótipos de plantas resistentes para serem usados em programas de melhoramento, considerando que a resistência será suplantada ou reduzida pelo surgimento de novas raças de A. psidii capazes de se adaptarem às defesas presente nos genótipos atualmente cultivados.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2024-03-20T13:18:18Z No. of bitstreams: 1 Tese - Renan Marcelo Portela.pdf: 1480463 bytes, checksum: 90f4771f53d44a88220f2550225daaef (MD5)Made available in DSpace on 2024-03-20T13:18:18Z (GMT). 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dc.title.por.fl_str_mv |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
title |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
spellingShingle |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 PORTELA, RENAN MARCELO Plantios florestais – produtividade Patógenos Ferrugem – eucalipto Forest plantings – productivity Pathogens Rust – eucalyptus CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::MANEJO FLORESTAL |
title_short |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
title_full |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
title_fullStr |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
title_full_unstemmed |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
title_sort |
INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017 |
author |
PORTELA, RENAN MARCELO |
author_facet |
PORTELA, RENAN MARCELO |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Garcia, Flávio Augusto de Oliveira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9237744351905960 |
dc.contributor.advisor-co1.fl_str_mv |
Tambarussi, Evandro Vagner |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3021997145272130 |
dc.contributor.advisor-co2.fl_str_mv |
Carolo Junior, Silvio |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/2262985790178752 |
dc.contributor.authorID.fl_str_mv |
084.267.289-36 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7069800805962679 |
dc.contributor.author.fl_str_mv |
PORTELA, RENAN MARCELO |
contributor_str_mv |
Garcia, Flávio Augusto de Oliveira Tambarussi, Evandro Vagner Carolo Junior, Silvio |
dc.subject.por.fl_str_mv |
Plantios florestais – produtividade Patógenos Ferrugem – eucalipto |
topic |
Plantios florestais – produtividade Patógenos Ferrugem – eucalipto Forest plantings – productivity Pathogens Rust – eucalyptus CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::MANEJO FLORESTAL |
dc.subject.eng.fl_str_mv |
Forest plantings – productivity Pathogens Rust – eucalyptus |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::MANEJO FLORESTAL |
description |
Forest-based activity plays an important socioeconomic role in the country. Currently, Brazil is the leader in the rank of forest productivity, however, this high productivity has been affected by the constant attacks of pathogens that cause economic damage to these crops, an example is the myrtle rust caused by Austropuccinia psidii (G. Winter) Beenken, which has a wide geographic distribution and presents great genetic variability among its populations, attacking several species of the Myrtaceae family. In this sense, we sought to study population-genetic and evolutionary aspects of A. psidii populations. These data were about microsatellite loci for obtaining A. psidii isolates in about 148 populations of plants, from which they originated anddeposited in DRYAD. The model used in the estimates was of four subpopulations of A. psidii grouped based on seven hosts of the Myrtaceae family, being SPOP1 (Eucalyptus spp. + S. jambos), SPOP2 (P. guajava + P. guineense), SPOP3 (S. cumini) and SPOP4 (M. cauliflora and E. uniflora). These populations were formed from collections carried out by the authors of the deposits in the states of Bahia, Espírito Santo, Mato Grosso do Sul, Minas Gerais, Rio de Janeiro, Paraná, Santa Catarina, São Paulo, Rio Grande do Sul, in addition to Uruguay. Effective size and gene flow were estimated using the Bayesian inference method, based on the coalescence theory implemented in the MIGRATE-N software. In addition to descriptive statistics and graphs of population allelic patterns, paired estimates of FST and intra and intergroup differentiation by analysis of molecular variance (AMOVA) considering the genetic groups of four subpopulations and the seven hosts in a hierarchical manner. Gene flow (Nm) between population pairs was low (all < 1 unit), and ranged from 0.04 to 0.67. Estimates of effective population size (N and) were 119, 111, 189 and 1315 for SPOP1, SPOP2, SPOP3 and SPOP4 respectively. AMOVA showed that the percentage of variation was 39.43% between subpopulations, 16.29% between hosts within subpopulations and 44.28% within hosts. FST analogues were Φ CT = 0.394 (Among Subpopulations); Φ SC = 0.269 (Among Hosts within Subpopulation); Φ ST = 0.556 (Within the Hosts), in addition to high paired FST values that ranged between 0.355 and 0.560. The results obtained add knowledge about the understanding of A. psidii population genetics, proposing plausible scenarios in relation to the evolutionary and demographic past, as well as the processes that gave rise to the observed variability. Possibly the subpopulations of A. psidii will have different ecological behaviors and represent different invasive threats, being indicated studies that can measure the invasive risks presented by the different subpopulations. The work emphasizes the evident importance of genetic conservation in an adequate way in commercial species, aiming to guarantee the existence of resistant plant genotypes to be used in breeding programs, considering that resistance will be supplanted or reduced by the emergence of new races of A. psidii capable of adapting to the defenses present in the genotypes currently cultivated. |
publishDate |
2023 |
dc.date.issued.fl_str_mv |
2023-02-17 |
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2024-03-20T13:18:18Z |
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PORTELA, RENAN MARCELO. INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017. 2023. 70 f. Tese (Programa de Pós-Graduação em Ciências Florestais - Doutorado) - Universidade Estadual do Centro-Oeste, Irati - PR. |
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http://tede.unicentro.br:8080/jspui/handle/jspui/2186 |
identifier_str_mv |
PORTELA, RENAN MARCELO. INFERÊNCIAS EVOLUTIVAS E IMPLICAÇÕES BIOMÉTRICAS EM Austropuccinia psidii (G. Winter) Beenken 2017. 2023. 70 f. Tese (Programa de Pós-Graduação em Ciências Florestais - Doutorado) - Universidade Estadual do Centro-Oeste, Irati - PR. |
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