An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae
Ano de defesa: | 2017 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Bot?nica
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/746 |
Resumo: | The pollen grains are a protein resource collected by bees for feeding larvae and adults inside the hive. During this collection the bee Apis mellifera L. deposits salivary substances, rich in enzymes and vitamins that enrich the pollen grains nutritionally and this happens to be denominated bee pollen. This bee product can be classified as monofloral or multifloral, depending on the botanical composition of the samples. Palm trees are currently represented by 181 genera and approximately 2,600 species. In Brazil, there are 38 genera and about 270 species. They are plants with great potential in the production of monofloral bee pollen. However, this classification requires some care, for example, the counting method used, since it is necessary to take into account the volume of the pollen units during the process of quantification of the pollen types. It is important to emphasize the recent studies with bee pollen have shown that chemical composition became a priority, because the presence of important nutrients and bioactive compounds, such as antioxidants such as flavonoids, carotenoids promoted a higher consumption of bee pollen on the market and became Target of greater interest by science. In this context, the main goal of study was to perform a pollen and chemical analysis of the samples of bee pollen with monofloral indication of Arecaceae produced in the region of Baixo Sul of Bahia, to relate the frequency and pollen volume to confirm or refute the indication of beekeepers and, finally, to determine the content of phenolics and antioxidant activity. The samples of monofloral bee pollen of Elaeis guineensis L, Cocos nucifera L. e Attalea funifera Mart. were submitted to the acetolysis process for pollen analysis and solvent extraction for chemical analyses. After the acetolysis, five permanent slides were mounted and counted at least 500 pollen grains per samples. For the volumetric analysis, 25 pollen units were used as the basis for the respective measurements and volumetric estimates with the aid of spatial geometry. The chemical analyzes were obtained from the ethanolic extracts and then the AcOEt fraction by partition. In order to determine the phenolic content, the Folin-Ciocalteau test was performed and for the antioxidant activity, DPPH and ABTS+ radicals were used. In the chemical analysis, fractions of ethanolic extracts (EtOH) and acetate (AcOEt) were produced, as well as the use radical free as DPPH and ABTS+ for the antioxidant tests. It is possible to state that pollen analysis is an essential tool in the botanical identification of bee pollen samples and that the classification proposed for the beekeepers is not reliable, since they are only empirical information. The volumetric analysis, besides pollen counting, was fundamental to determine the botanical origin, since it allowed to classify the samples analyzed in mono or multifloral. Thus, among the 25 analyzed samples of E. guineensis, 21 were classified as monofloral, among 13 analyzed of C. nucifera. Only three were not classified as monofloral and among the seven analyzed samples of A. funifera, only one was not considered monofloral. In relation to the samples of C. nucifera it was possible to determine its monofloral characteristic through the pollen count, already in relation to the type A. funifera it was necessary to perform volumetric analyzes to confirm this characteristic. Finally, in relation to the chemical analysis, it can be stated that the results were relevant for the bee product, despite the low values found for the phenolic content, being 1.5 ? 0.15 at 26.3 ? 0.48 mg GAE/g in the ETOH fraction and 8.3 ? 0.87 at 125.8 ? 0.93 mg GAE/g in the AcOEt fraction compared to the literature data. Samples of Elaeis guineensis L. showed an antioxidant activity ranging from 34.9 ? 0.53 to 588.2 ? 4.65 ?g / mL ? EPM in the DPPH method and from 20.8 ? 0.26 to 383, 9 ? 0.75 ?g / mL ? EPM in the ABTS + method. This information can be used as a form of certification in ensuring quality and adding value to the product |
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Santos, Francisco de Assis Ribeiro39444295591http://lattes.cnpq.br/390398866221905502291777513http://lattes.cnpq.br/2693490136067912Alves, Rodolfo de Fran?a2019-04-30T22:10:42Z2017-07-03ALVES, Rodolfo de Fran?a. An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae. 2017. 121f. Tese (Doutorado Acad?mico em Bot?nica) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017.http://tede2.uefs.br:8080/handle/tede/746The pollen grains are a protein resource collected by bees for feeding larvae and adults inside the hive. During this collection the bee Apis mellifera L. deposits salivary substances, rich in enzymes and vitamins that enrich the pollen grains nutritionally and this happens to be denominated bee pollen. This bee product can be classified as monofloral or multifloral, depending on the botanical composition of the samples. Palm trees are currently represented by 181 genera and approximately 2,600 species. In Brazil, there are 38 genera and about 270 species. They are plants with great potential in the production of monofloral bee pollen. However, this classification requires some care, for example, the counting method used, since it is necessary to take into account the volume of the pollen units during the process of quantification of the pollen types. It is important to emphasize the recent studies with bee pollen have shown that chemical composition became a priority, because the presence of important nutrients and bioactive compounds, such as antioxidants such as flavonoids, carotenoids promoted a higher consumption of bee pollen on the market and became Target of greater interest by science. In this context, the main goal of study was to perform a pollen and chemical analysis of the samples of bee pollen with monofloral indication of Arecaceae produced in the region of Baixo Sul of Bahia, to relate the frequency and pollen volume to confirm or refute the indication of beekeepers and, finally, to determine the content of phenolics and antioxidant activity. The samples of monofloral bee pollen of Elaeis guineensis L, Cocos nucifera L. e Attalea funifera Mart. were submitted to the acetolysis process for pollen analysis and solvent extraction for chemical analyses. After the acetolysis, five permanent slides were mounted and counted at least 500 pollen grains per samples. For the volumetric analysis, 25 pollen units were used as the basis for the respective measurements and volumetric estimates with the aid of spatial geometry. The chemical analyzes were obtained from the ethanolic extracts and then the AcOEt fraction by partition. In order to determine the phenolic content, the Folin-Ciocalteau test was performed and for the antioxidant activity, DPPH and ABTS+ radicals were used. In the chemical analysis, fractions of ethanolic extracts (EtOH) and acetate (AcOEt) were produced, as well as the use radical free as DPPH and ABTS+ for the antioxidant tests. It is possible to state that pollen analysis is an essential tool in the botanical identification of bee pollen samples and that the classification proposed for the beekeepers is not reliable, since they are only empirical information. The volumetric analysis, besides pollen counting, was fundamental to determine the botanical origin, since it allowed to classify the samples analyzed in mono or multifloral. Thus, among the 25 analyzed samples of E. guineensis, 21 were classified as monofloral, among 13 analyzed of C. nucifera. Only three were not classified as monofloral and among the seven analyzed samples of A. funifera, only one was not considered monofloral. In relation to the samples of C. nucifera it was possible to determine its monofloral characteristic through the pollen count, already in relation to the type A. funifera it was necessary to perform volumetric analyzes to confirm this characteristic. Finally, in relation to the chemical analysis, it can be stated that the results were relevant for the bee product, despite the low values found for the phenolic content, being 1.5 ? 0.15 at 26.3 ? 0.48 mg GAE/g in the ETOH fraction and 8.3 ? 0.87 at 125.8 ? 0.93 mg GAE/g in the AcOEt fraction compared to the literature data. Samples of Elaeis guineensis L. showed an antioxidant activity ranging from 34.9 ? 0.53 to 588.2 ? 4.65 ?g / mL ? EPM in the DPPH method and from 20.8 ? 0.26 to 383, 9 ? 0.75 ?g / mL ? EPM in the ABTS + method. This information can be used as a form of certification in ensuring quality and adding value to the productO p?len ? um recurso proteico coletado pelas abelhas para alimenta??o das larvas e adultos dentro da colmeia. Durante essa coleta a abelha Apis mellifera L. deposita subst?ncias salivares, ricas em enzimas e vitaminas que enriquecem os gr?os de p?len nutricionalmente e este passa a ser denominado p?len ap?cola. Esse produto ap?cola pode ser classificado como monofloral ou multifloral, a depender da composi??o bot?nica das amostras. As palmeiras s?o representadas atualmente por 181 g?neros e, aproximadamente, 2.600 esp?cies. No Brasil, ocorrem 38 g?neros e cerca de 270 esp?cies. S?o plantas com grande potencial na produ??o de p?len ap?cola monofloral. ? importante ressaltar os ?ltimos estudos com p?len ap?cola t?m mostrado que a composi??o qu?mica se tornou prioridade, pois a presen?a de importantes nutrientes e compostos bioativos, como substancias antioxidantes, como flavonoides, carotenoides promoveu um maior consumo do p?len ap?cola no mercado e passou a ser alvo de maior interesse pela ci?ncia. Nesse contexto, o objetivo central do estudo foi realizar uma an?lise pol?nica e determina??o do teor de fen?licos e atividade antioxidante das amostras de p?len ap?cola com indica??o monofloral de Arecaceae produzidas na regi?o do Baixo Sul da Bahia, relacionar a frequ?ncia e o volume pol?nico para confirmar ou refutar a indica??o dos apicultores quanto ? origem bot?nica e, por fim, determinar o teor de fen?licos e atividade antioxidante. As amostras de p?len ap?cola monofloral de Elaeis guineensis L., Cocos nucifera L. e Attalea funifera Mart. foram submetidas ao processo de acet?lise para as an?lises pol?nicas e aos processos de extra??o com solventes org?nicos para as an?lises qu?micas. Ap?s a acet?lise foram montadas cinco l?minas permanentes e contabilizados, no m?nimo, 500 gr?os de p?len por amostra. Para as an?lises volum?tricas foram utilizadas 25 unidades pol?nicas como base para as respectivas medi??es e estimativas volum?tricas com o aux?lio da geometria espacial. As an?lises qu?micas foram obtidas a partir dos extratos etan?licos e, em seguida a fra??o AcOEt por parti??o. Para determina??o do teor de fen?licos foi feito o ensaio com Folin-Ciocalteau e para a atividade antioxidante, foram utilizados os radicais DPPH e ABTS+. Os resultados confirmaram que a an?lise pol?nica ? uma ferramenta essencial na identifica??o bot?nica das amostras de p?len ap?cola e que a classifica??o proposta pelos apicultores n?o ? totalmente confi?vel, pois s?o informa??es de cunho apenas emp?rico. As an?lises volum?tricas, al?m da contagem pol?nica, foram fundamentais para determina??o da origem bot?nica, pois permitiu classificar as amostras analisadas em mono ou multifloral. Assim, dentre as 25 amostras analisadas de E. guineensis, 21 foram classificadas como monofloral, dentre as 13 analisadas de C. nucifera. Somente tr?s n?o foram classificadas como monofloral e entre as sete amostras analisadas de A. funifera, somente uma n?o foi considerada monofloral. Em rela??o ?s amostras de Cocos nucifera foi poss?vel determinar sua caracter?stica monofloral atrav?s da contagem pol?nica, j? em rela??o ao tipo Attalea funifera foi preciso realizar an?lises volum?tricas para a confirma??o dessa caracter?stica. Por fim, em rela??o ?s an?lises qu?micas, pode-se afirmar que os resultados foram relevantes para o produto ap?cola, apesar dos baixos valores encontrados para o teor de fen?licos, sendo 1,5?0,15 a 26,3?0,48 mg GAE/g na fra??o ETOH e 8,3?0,87 a 125,8?0,93 mg GAE/g na fra??o AcOEt comparado com os dados da literatura. As amostras de Elaeis guineensis L. apresentaram uma atividade antioxidante com varia??es de 34,9?0,53 a 588,2?4,65 ?g/mL?EPM no m?todo DPPH e de 20,8?0,26 a 383,9?0,75 ?g/mL?EPM no m?todo ABTS+. Essas informa??es podem ser utilizadas como uma forma de certifica??o na garantia de qualidade e agrega??o de valor ao produtoSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2019-04-30T22:10:42Z No. of bitstreams: 1 Tese_Rodolfo _PDF.pdf: 13686614 bytes, checksum: 6f54948e5a5d8fa2c3eb2e060568b58b (MD5)Made available in DSpace on 2019-04-30T22:10:42Z (GMT). No. of bitstreams: 1 Tese_Rodolfo _PDF.pdf: 13686614 bytes, checksum: 6f54948e5a5d8fa2c3eb2e060568b58b (MD5) Previous issue date: 2017-07-03Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em Bot?nicaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASApiculturaApis mellifera L.palinologiaunifloralvolumeApiculture; Apis mellifera L.; palynology; unifloral; volume.Apis mellifera L.palynologyunifloralvolumeBOTANICA::BOTANICA APLICADAAn?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-4908229543721086191600600600600502612338345058928259250118293197842713590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTese_Rodolfo _PDF.pdfTese_Rodolfo _PDF.pdfapplication/pdf13686614http://tede2.uefs.br:8080/bitstream/tede/746/2/Tese_Rodolfo+_PDF.pdf6f54948e5a5d8fa2c3eb2e060568b58bMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/746/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/7462019-04-30 19:10:42.107oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-04-30T22:10:42Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
title |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
spellingShingle |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae Alves, Rodolfo de Fran?a Apicultura Apis mellifera L. palinologia unifloral volume Apiculture; Apis mellifera L.; palynology; unifloral; volume. Apis mellifera L. palynology unifloral volume BOTANICA::BOTANICA APLICADA |
title_short |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
title_full |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
title_fullStr |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
title_full_unstemmed |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
title_sort |
An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae |
author |
Alves, Rodolfo de Fran?a |
author_facet |
Alves, Rodolfo de Fran?a |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santos, Francisco de Assis Ribeiro |
dc.contributor.advisor1ID.fl_str_mv |
39444295591 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3903988662219055 |
dc.contributor.authorID.fl_str_mv |
02291777513 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2693490136067912 |
dc.contributor.author.fl_str_mv |
Alves, Rodolfo de Fran?a |
contributor_str_mv |
Santos, Francisco de Assis Ribeiro |
dc.subject.por.fl_str_mv |
Apicultura Apis mellifera L. palinologia unifloral volume |
topic |
Apicultura Apis mellifera L. palinologia unifloral volume Apiculture; Apis mellifera L.; palynology; unifloral; volume. Apis mellifera L. palynology unifloral volume BOTANICA::BOTANICA APLICADA |
dc.subject.eng.fl_str_mv |
Apiculture; Apis mellifera L.; palynology; unifloral; volume. Apis mellifera L. palynology unifloral volume |
dc.subject.cnpq.fl_str_mv |
BOTANICA::BOTANICA APLICADA |
description |
The pollen grains are a protein resource collected by bees for feeding larvae and adults inside the hive. During this collection the bee Apis mellifera L. deposits salivary substances, rich in enzymes and vitamins that enrich the pollen grains nutritionally and this happens to be denominated bee pollen. This bee product can be classified as monofloral or multifloral, depending on the botanical composition of the samples. Palm trees are currently represented by 181 genera and approximately 2,600 species. In Brazil, there are 38 genera and about 270 species. They are plants with great potential in the production of monofloral bee pollen. However, this classification requires some care, for example, the counting method used, since it is necessary to take into account the volume of the pollen units during the process of quantification of the pollen types. It is important to emphasize the recent studies with bee pollen have shown that chemical composition became a priority, because the presence of important nutrients and bioactive compounds, such as antioxidants such as flavonoids, carotenoids promoted a higher consumption of bee pollen on the market and became Target of greater interest by science. In this context, the main goal of study was to perform a pollen and chemical analysis of the samples of bee pollen with monofloral indication of Arecaceae produced in the region of Baixo Sul of Bahia, to relate the frequency and pollen volume to confirm or refute the indication of beekeepers and, finally, to determine the content of phenolics and antioxidant activity. The samples of monofloral bee pollen of Elaeis guineensis L, Cocos nucifera L. e Attalea funifera Mart. were submitted to the acetolysis process for pollen analysis and solvent extraction for chemical analyses. After the acetolysis, five permanent slides were mounted and counted at least 500 pollen grains per samples. For the volumetric analysis, 25 pollen units were used as the basis for the respective measurements and volumetric estimates with the aid of spatial geometry. The chemical analyzes were obtained from the ethanolic extracts and then the AcOEt fraction by partition. In order to determine the phenolic content, the Folin-Ciocalteau test was performed and for the antioxidant activity, DPPH and ABTS+ radicals were used. In the chemical analysis, fractions of ethanolic extracts (EtOH) and acetate (AcOEt) were produced, as well as the use radical free as DPPH and ABTS+ for the antioxidant tests. It is possible to state that pollen analysis is an essential tool in the botanical identification of bee pollen samples and that the classification proposed for the beekeepers is not reliable, since they are only empirical information. The volumetric analysis, besides pollen counting, was fundamental to determine the botanical origin, since it allowed to classify the samples analyzed in mono or multifloral. Thus, among the 25 analyzed samples of E. guineensis, 21 were classified as monofloral, among 13 analyzed of C. nucifera. Only three were not classified as monofloral and among the seven analyzed samples of A. funifera, only one was not considered monofloral. In relation to the samples of C. nucifera it was possible to determine its monofloral characteristic through the pollen count, already in relation to the type A. funifera it was necessary to perform volumetric analyzes to confirm this characteristic. Finally, in relation to the chemical analysis, it can be stated that the results were relevant for the bee product, despite the low values found for the phenolic content, being 1.5 ? 0.15 at 26.3 ? 0.48 mg GAE/g in the ETOH fraction and 8.3 ? 0.87 at 125.8 ? 0.93 mg GAE/g in the AcOEt fraction compared to the literature data. Samples of Elaeis guineensis L. showed an antioxidant activity ranging from 34.9 ? 0.53 to 588.2 ? 4.65 ?g / mL ? EPM in the DPPH method and from 20.8 ? 0.26 to 383, 9 ? 0.75 ?g / mL ? EPM in the ABTS + method. This information can be used as a form of certification in ensuring quality and adding value to the product |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-07-03 |
dc.date.accessioned.fl_str_mv |
2019-04-30T22:10:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALVES, Rodolfo de Fran?a. An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae. 2017. 121f. Tese (Doutorado Acad?mico em Bot?nica) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/746 |
identifier_str_mv |
ALVES, Rodolfo de Fran?a. An?lise pol?nica e qu?mica de amostras de p?len ap?cola monofloral de esp?cies da fam?lia Arecaceae. 2017. 121f. Tese (Doutorado Acad?mico em Bot?nica) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017. |
url |
http://tede2.uefs.br:8080/handle/tede/746 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-4908229543721086191 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
5026123383450589282 |
dc.relation.cnpq.fl_str_mv |
5925011829319784271 |
dc.relation.sponsorship.fl_str_mv |
3590462550136975366 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acad?mico em Bot?nica |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
instname_str |
Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/746/2/Tese_Rodolfo+_PDF.pdf http://tede2.uefs.br:8080/bitstream/tede/746/1/license.txt |
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MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1796793637472829440 |