Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Mestrado Acad?mico em Biotecnologia
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/1368 |
Resumo: | Neuropathic pain is caused by dysfunction of the nervous system and affects about 5% of the world. Its control is still inefficient and only a third of patients undergoing therapy existing features more than 50% pain relief. This study investigated the potential of cell therapy for recovery from nervous system function in experimental neuropathic pain. We use the model of partial sciatic nerve ligation in mice and evaluated the mechanical allodynia with von Frey filaments. Mice were treated intravenously with mononuclear cells obtained from bone marrow (BMMC), soluble factors resulting from the lysate of these cells and cell populations of the same origin adherent and non-adherent to polymers. Treatment with BMMC was also administered to animals for the gene knockout of the cytokine IL-10. Levels of cytokines IL-1?, IL-6, IL-10 and TNF-? were measured in sections of the sciatic nerve and spinal cord. The markup for GFAP in Schwann cells was evaluated in experimental groups. As a result, the administration of BMMC reduced allodynia and the expression of cytokines typically pro-inflammatory IL-1? and TNF-?, with increased expression of anti-inflammatory cytokine IL-10 in sciatic nerve and spinal cord. In knockout animals IL-10 treatment was ineffective. In addition, animals treated with BMMC showed a lower number of Schwann cells, involved in the production of noxious factors in the sciatic nerve. These results emphasize the potential of cell therapy for neuropathic pain management with the participation of soluble factors, mainly IL-10. |
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Villarreal, Cristiane Flora8958036265380470483504http://lattes.cnpq.br/9384130510751320Almeida, Ta?s Adelita Morais de2022-05-24T21:25:49Z2010-04-22BARROS, Ta?s Adelita de Almeida. Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica. 2010. 91 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.http://tede2.uefs.br:8080/handle/tede/1368Neuropathic pain is caused by dysfunction of the nervous system and affects about 5% of the world. Its control is still inefficient and only a third of patients undergoing therapy existing features more than 50% pain relief. This study investigated the potential of cell therapy for recovery from nervous system function in experimental neuropathic pain. We use the model of partial sciatic nerve ligation in mice and evaluated the mechanical allodynia with von Frey filaments. Mice were treated intravenously with mononuclear cells obtained from bone marrow (BMMC), soluble factors resulting from the lysate of these cells and cell populations of the same origin adherent and non-adherent to polymers. Treatment with BMMC was also administered to animals for the gene knockout of the cytokine IL-10. Levels of cytokines IL-1?, IL-6, IL-10 and TNF-? were measured in sections of the sciatic nerve and spinal cord. The markup for GFAP in Schwann cells was evaluated in experimental groups. As a result, the administration of BMMC reduced allodynia and the expression of cytokines typically pro-inflammatory IL-1? and TNF-?, with increased expression of anti-inflammatory cytokine IL-10 in sciatic nerve and spinal cord. In knockout animals IL-10 treatment was ineffective. In addition, animals treated with BMMC showed a lower number of Schwann cells, involved in the production of noxious factors in the sciatic nerve. These results emphasize the potential of cell therapy for neuropathic pain management with the participation of soluble factors, mainly IL-10.Dor neurop?tica ? causada por disfun??o do sistema nervoso e acomete cerca de 5% da popula??o mundial. Seu controle ? ainda ineficiente e apenas um ter?o dos pacientes submetidos ? terapia vigente apresenta mais de 50% de al?vio da dor. O presente trabalho investigou a potencialidade da terapia celular em recuperar a fun??o do sistema nervoso na dor neurop?tica experimental. Foi utilizado o modelo de ligadura parcial do nervo isqui?tico em camundongos e avaliou-se a alodinia mec?nica com filamentos de von Frey. Camundongos foram tratados por via endovenosa com c?lulas mononucleares de medula ?ssea (CMMO) murina, fatores sol?veis resultantes do lisado destas c?lulas e popula??es celulares de mesma origem aderente e n?o-aderente a pol?meros. O tratamento com CMMO tamb?m foi administrado a animais knock out para o gene da citocina IL-10. Os n?veis das citocinas IL-1?, IL-6, IL-10 e TNF-? foram dosados em sec??es do nervo isqui?tico e medula espinal. A marca??o para GFAP em c?lulas de Schwann tamb?m foi avaliada nos grupos experimentais. Como resultados, a administra??o de CMMO reduziu a alodinia bem como a express?o da citocina TNF-?, com aumento da express?o da citocina anti-inflamat?ria IL-10, no nervo isqui?tico e na medula espinal. Em animais knock out para IL-10 o tratamento n?o foi efetivo. Em adi??o, animais tratados com CMMO apresentaram menor express?o de c?lulas de Schwann, envolvidas com a produ??o de fatores nociceptivos, no nervo isqui?tico. Esses resultados refor?am o potencial da terapia celular para o controle da dor neurop?tica com participa??o de fatores sol?veis, principalmente IL-10.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2022-05-24T21:25:49Z No. of bitstreams: 1 Disserta??o Ta?s Adelita de Almeida Barros.pdf: 1298571 bytes, checksum: 940472def5ccc573bca9854908c07fc2 (MD5)Made available in DSpace on 2022-05-24T21:25:49Z (GMT). No. of bitstreams: 1 Disserta??o Ta?s Adelita de Almeida Barros.pdf: 1298571 bytes, checksum: 940472def5ccc573bca9854908c07fc2 (MD5) Previous issue date: 2010-04-22Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPqapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASDor neurop?ticaTerapia CelularC?lulas mononuclearesCitocinasAlodinia mec?nicaMedula ?sseaBone marrow mononuclear cellsCell therapyCytokinesMechanical allodyniaStem cellsNeuropathic painCIENCIAS BIOLOGICASAvalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?ticainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-54735432730516527826006006006005026123383450589282-34391788430682021611802873727776104890info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDisserta??o Ta?s Adelita de Almeida Barros.pdfDisserta??o Ta?s Adelita de Almeida Barros.pdfapplication/pdf1298571http://tede2.uefs.br:8080/bitstream/tede/1368/2/Disserta%C3%A7%C3%A3o+Ta%C3%ADs+Adelita+de+Almeida+Barros.pdf940472def5ccc573bca9854908c07fc2MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/1368/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/13682022-05-24 18:25:49.239oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2022-05-24T21:25:49Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
title |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
spellingShingle |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica Almeida, Ta?s Adelita Morais de Dor neurop?tica Terapia Celular C?lulas mononucleares Citocinas Alodinia mec?nica Medula ?ssea Bone marrow mononuclear cells Cell therapy Cytokines Mechanical allodynia Stem cells Neuropathic pain CIENCIAS BIOLOGICAS |
title_short |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
title_full |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
title_fullStr |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
title_full_unstemmed |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
title_sort |
Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica |
author |
Almeida, Ta?s Adelita Morais de |
author_facet |
Almeida, Ta?s Adelita Morais de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Villarreal, Cristiane Flora |
dc.contributor.advisor1ID.fl_str_mv |
89580362653 |
dc.contributor.authorID.fl_str_mv |
80470483504 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9384130510751320 |
dc.contributor.author.fl_str_mv |
Almeida, Ta?s Adelita Morais de |
contributor_str_mv |
Villarreal, Cristiane Flora |
dc.subject.por.fl_str_mv |
Dor neurop?tica Terapia Celular C?lulas mononucleares Citocinas Alodinia mec?nica Medula ?ssea |
topic |
Dor neurop?tica Terapia Celular C?lulas mononucleares Citocinas Alodinia mec?nica Medula ?ssea Bone marrow mononuclear cells Cell therapy Cytokines Mechanical allodynia Stem cells Neuropathic pain CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Bone marrow mononuclear cells Cell therapy Cytokines Mechanical allodynia Stem cells Neuropathic pain |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS |
description |
Neuropathic pain is caused by dysfunction of the nervous system and affects about 5% of the world. Its control is still inefficient and only a third of patients undergoing therapy existing features more than 50% pain relief. This study investigated the potential of cell therapy for recovery from nervous system function in experimental neuropathic pain. We use the model of partial sciatic nerve ligation in mice and evaluated the mechanical allodynia with von Frey filaments. Mice were treated intravenously with mononuclear cells obtained from bone marrow (BMMC), soluble factors resulting from the lysate of these cells and cell populations of the same origin adherent and non-adherent to polymers. Treatment with BMMC was also administered to animals for the gene knockout of the cytokine IL-10. Levels of cytokines IL-1?, IL-6, IL-10 and TNF-? were measured in sections of the sciatic nerve and spinal cord. The markup for GFAP in Schwann cells was evaluated in experimental groups. As a result, the administration of BMMC reduced allodynia and the expression of cytokines typically pro-inflammatory IL-1? and TNF-?, with increased expression of anti-inflammatory cytokine IL-10 in sciatic nerve and spinal cord. In knockout animals IL-10 treatment was ineffective. In addition, animals treated with BMMC showed a lower number of Schwann cells, involved in the production of noxious factors in the sciatic nerve. These results emphasize the potential of cell therapy for neuropathic pain management with the participation of soluble factors, mainly IL-10. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-04-22 |
dc.date.accessioned.fl_str_mv |
2022-05-24T21:25:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARROS, Ta?s Adelita de Almeida. Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica. 2010. 91 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1368 |
identifier_str_mv |
BARROS, Ta?s Adelita de Almeida. Avalia??o dos efeitos da terapia celular em modelo experimental de dor neurop?tica. 2010. 91 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010. |
url |
http://tede2.uefs.br:8080/handle/tede/1368 |
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por |
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por |
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600 600 600 600 |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Mestrado Acad?mico em Biotecnologia |
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UEFS |
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Brasil |
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DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
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Universidade Estadual de Feira de Santana |
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