Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Biotecnologia
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/1505 |
Resumo: | This study will be divided into chapters. The first chapter aims to investigate the chemical constituents and biological activities of aerial parts of Bromelia laciniosa. For this, the chemical composition of the chloroform (Bl-CHCl3), ethyl acetate (Bl-AcOEt) and ethanolic (Bl-EtOH) extracts from the leaves of B. laciniosa were evaluated by HPLC-DAD-ESI-MS/MS and by NMR. The contents of total phenols, toxicity and antioxidant activities, in vitro, of the extracts were also evaluated. In the analysis by HPLC-DAD-IES-EM/EM, 18 constituents were identified, especially phenylpropanoids, mono and diglycerides, while by NMR, it resulted in the identification of 1,3-O-dicafeoylglycerol, the major compound of the Bl-AcOEt extract, which is highlighted for its antioxidant and antibacterial potential against S. aureus MRSA. The second chapter involved the steps of preparation and evaluation of the biological potential of formulations, with the objective of developing herbal formulations containing B. laciniosa extracts with antibacterial action against strains of S. aureus MRSA. Thus, the bioactive phenolic profile of the species was described by HPLC-DAD, and the antibacterial formulations of Lanette WB? were developed from extracts of B. laciniosa leaves, also evaluating the antioxidant and antibacterial activities of the preparations. Formulation F6 (10% of Bl-AcOEt) exhibited pronounced antioxidant effect and showed anti-staphylococcal activity against three clinical isolates of S. aureus MRSA and standard strain, exhibiting a superior effect to the reference antimicrobial. The results confirm the promising potential of B. laciniosa as a source of drugs for the treatment of bacterial infections. This is the first report on or development of pharmaceutical preparations containing extract of B. laciniosa. |
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Almeida, Jackson Roberto Guedes da Silva03016512483http://lattes.cnpq.br/676274200266025104891909528http://lattes.cnpq.br/6148240648263271Pontes, Michelle Cruz2023-08-09T13:44:01Z2021-10-15PONTES, Michelle Cruz. Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus. 2021. 101f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2021.http://tede2.uefs.br:8080/handle/tede/1505This study will be divided into chapters. The first chapter aims to investigate the chemical constituents and biological activities of aerial parts of Bromelia laciniosa. For this, the chemical composition of the chloroform (Bl-CHCl3), ethyl acetate (Bl-AcOEt) and ethanolic (Bl-EtOH) extracts from the leaves of B. laciniosa were evaluated by HPLC-DAD-ESI-MS/MS and by NMR. The contents of total phenols, toxicity and antioxidant activities, in vitro, of the extracts were also evaluated. In the analysis by HPLC-DAD-IES-EM/EM, 18 constituents were identified, especially phenylpropanoids, mono and diglycerides, while by NMR, it resulted in the identification of 1,3-O-dicafeoylglycerol, the major compound of the Bl-AcOEt extract, which is highlighted for its antioxidant and antibacterial potential against S. aureus MRSA. The second chapter involved the steps of preparation and evaluation of the biological potential of formulations, with the objective of developing herbal formulations containing B. laciniosa extracts with antibacterial action against strains of S. aureus MRSA. Thus, the bioactive phenolic profile of the species was described by HPLC-DAD, and the antibacterial formulations of Lanette WB? were developed from extracts of B. laciniosa leaves, also evaluating the antioxidant and antibacterial activities of the preparations. Formulation F6 (10% of Bl-AcOEt) exhibited pronounced antioxidant effect and showed anti-staphylococcal activity against three clinical isolates of S. aureus MRSA and standard strain, exhibiting a superior effect to the reference antimicrobial. The results confirm the promising potential of B. laciniosa as a source of drugs for the treatment of bacterial infections. This is the first report on or development of pharmaceutical preparations containing extract of B. laciniosa.O presente estudo foi dividido em dois cap?tulos. O primeiro cap?tulo teve como objetivo investigar os constituintes qu?micos e as atividades biol?gicas de partes a?reas de Bromelia laciniosa. Para isso, a composi??o qu?mica dos extratos clorof?rmico (Bl-CHCl3), acetato de etila (Bl-AcOEt) e etan?lico (Bl-EtOH) foram avaliados por CLAE-DAD-IES-EM/EM e RMN. Tamb?m foram avaliados os teores de fen?is totais, toxicidade e atividade antioxidante in vitro dos extratos. Na an?lise por CLAE-DAD-IES-EM/EM foram identificados 18 constituintes, especialmente fenilpropanoides, mono e diglicer?deos, enquanto por RMN, resultou na identifica??o de 1,3-O-dicafeoilglicerol, composto majorit?rio do extrato Bl-AcOEt, que se destacou por seu potencial antioxidante e antibacteriano frente ? S. aureus MRSA. O segundo cap?tulo envolveu as etapas de prepara??o e avalia??o do potencial biol?gico das formula??es, tendo como objetivo desenvolver formula??es fitoter?picas, contendo extratos de B. laciniosa, com a??o antibacteriana contra cepas de S. aureus MRSA. Assim, o perfil fen?lico bioativo da esp?cie foi descrito por CLAE-DAD, e as formula??es antibacterianas de Lanette WB? foram desenvolvidas a partir de extratos de folhas de B. laciniosa, avaliando tamb?m as atividades antioxidante e antibacteriana dos preparados. A formula??o F6 (10% de Bl-AcOEt) exibiu acentuado efeito antioxidante e apresentou atividade anti-estafiloc?cica contra tr?s isolados cl?nicos de S. aureus MRSA e cepa padr?o, exibindo um efeito superior ao antimicrobiano de refer?ncia. Os resultados dessa pesquisa, comprovam o potencial promissor de B. laciniosa como fonte de medicamentos para o tratamento de infec??es bacterianas. Esta ? a primeira pesquisa sobre o desenvolvimento de prepara??es farmac?uticas contendo extrato de B. laciniosa.Submitted by Amanda Ponce (aponce@uefs.br) on 2023-08-09T13:44:01Z No. of bitstreams: 1 Termo_de_autorizacao_Michelle_Cruz.pdf: 1753502 bytes, checksum: a6100f10d9031ad9ba25101a4b158b18 (MD5)Made available in DSpace on 2023-08-09T13:44:01Z (GMT). No. of bitstreams: 1 Termo_de_autorizacao_Michelle_Cruz.pdf: 1753502 bytes, checksum: a6100f10d9031ad9ba25101a4b158b18 (MD5) Previous issue date: 2021-10-15Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASBromeliaceaeBromeliaCaracteriza??o qu?micaAtividade antibacterianaFormula??o antibacterianaStaphylococcus aureus MRSAChemical characterizationAntibacterial activityAntioxidant activity,BIOQUIMICA::BIOLOGIA MOLECULARCIENCIAS BIOLOGICAS::FARMACOLOGIACIENCIAS BIOLOGICAS::MICROBIOLOGIADesenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-5520924549352904568600600600600600600-69714807220085378723962143990328052072700814650651154363-38545834699762208122075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTermo_de_autorizacao_Michelle_Cruz.pdfTermo_de_autorizacao_Michelle_Cruz.pdfapplication/pdf1753502http://tede2.uefs.br:8080/bitstream/tede/1505/2/Termo_de_autorizacao_Michelle_Cruz.pdfa6100f10d9031ad9ba25101a4b158b18MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede2.uefs.br:8080/bitstream/tede/1505/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/15052023-08-09 10:44:01.427oai:tede2.uefs.br:8080:tede/1505Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2023-08-09T13:44:01Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
title |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
spellingShingle |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus Pontes, Michelle Cruz Bromeliaceae Bromelia Caracteriza??o qu?mica Atividade antibacteriana Formula??o antibacteriana Staphylococcus aureus MRSA Chemical characterization Antibacterial activity Antioxidant activity, BIOQUIMICA::BIOLOGIA MOLECULAR CIENCIAS BIOLOGICAS::FARMACOLOGIA CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
title_short |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
title_full |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
title_fullStr |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
title_full_unstemmed |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
title_sort |
Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus |
author |
Pontes, Michelle Cruz |
author_facet |
Pontes, Michelle Cruz |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Almeida, Jackson Roberto Guedes da Silva |
dc.contributor.advisor1ID.fl_str_mv |
03016512483 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6762742002660251 |
dc.contributor.authorID.fl_str_mv |
04891909528 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6148240648263271 |
dc.contributor.author.fl_str_mv |
Pontes, Michelle Cruz |
contributor_str_mv |
Almeida, Jackson Roberto Guedes da Silva |
dc.subject.por.fl_str_mv |
Bromeliaceae Bromelia Caracteriza??o qu?mica Atividade antibacteriana Formula??o antibacteriana Staphylococcus aureus MRSA |
topic |
Bromeliaceae Bromelia Caracteriza??o qu?mica Atividade antibacteriana Formula??o antibacteriana Staphylococcus aureus MRSA Chemical characterization Antibacterial activity Antioxidant activity, BIOQUIMICA::BIOLOGIA MOLECULAR CIENCIAS BIOLOGICAS::FARMACOLOGIA CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
dc.subject.eng.fl_str_mv |
Chemical characterization Antibacterial activity Antioxidant activity, |
dc.subject.cnpq.fl_str_mv |
BIOQUIMICA::BIOLOGIA MOLECULAR CIENCIAS BIOLOGICAS::FARMACOLOGIA CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
description |
This study will be divided into chapters. The first chapter aims to investigate the chemical constituents and biological activities of aerial parts of Bromelia laciniosa. For this, the chemical composition of the chloroform (Bl-CHCl3), ethyl acetate (Bl-AcOEt) and ethanolic (Bl-EtOH) extracts from the leaves of B. laciniosa were evaluated by HPLC-DAD-ESI-MS/MS and by NMR. The contents of total phenols, toxicity and antioxidant activities, in vitro, of the extracts were also evaluated. In the analysis by HPLC-DAD-IES-EM/EM, 18 constituents were identified, especially phenylpropanoids, mono and diglycerides, while by NMR, it resulted in the identification of 1,3-O-dicafeoylglycerol, the major compound of the Bl-AcOEt extract, which is highlighted for its antioxidant and antibacterial potential against S. aureus MRSA. The second chapter involved the steps of preparation and evaluation of the biological potential of formulations, with the objective of developing herbal formulations containing B. laciniosa extracts with antibacterial action against strains of S. aureus MRSA. Thus, the bioactive phenolic profile of the species was described by HPLC-DAD, and the antibacterial formulations of Lanette WB? were developed from extracts of B. laciniosa leaves, also evaluating the antioxidant and antibacterial activities of the preparations. Formulation F6 (10% of Bl-AcOEt) exhibited pronounced antioxidant effect and showed anti-staphylococcal activity against three clinical isolates of S. aureus MRSA and standard strain, exhibiting a superior effect to the reference antimicrobial. The results confirm the promising potential of B. laciniosa as a source of drugs for the treatment of bacterial infections. This is the first report on or development of pharmaceutical preparations containing extract of B. laciniosa. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-10-15 |
dc.date.accessioned.fl_str_mv |
2023-08-09T13:44:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PONTES, Michelle Cruz. Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus. 2021. 101f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2021. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1505 |
identifier_str_mv |
PONTES, Michelle Cruz. Desenvolvimento de formula??o contendo extratos de Bromelia laciniosa com atividade antibacteriana frente a cepas de Staphylococcus aureus. 2021. 101f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2021. |
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http://tede2.uefs.br:8080/handle/tede/1505 |
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por |
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por |
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openAccess |
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Universidade Estadual de Feira de Santana |
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