Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose

Detalhes bibliográficos
Ano de defesa: 2010
Autor(a) principal: Nascimento, Diego Sampaio lattes
Orientador(a): Assis, Sandra Aparecida de
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Feira de Santana
Programa de Pós-Graduação: Mestrado Acad?mico em Biotecnologia
Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
País: Brasil
Palavras-chave em Português:
Inulina
inulinase
leveduras
semi-?rido
Brasil
Palavras-chave em Inglês:
Inulin
inulinase
yeasts
semi-arid
Brazil
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
Link de acesso: http://tede2.uefs.br:8080/handle/tede/1192
Resumo: Inulinases target the ?-2 linkage of inulin, a poliyfructan consisting of linear ?-2,1 linked fructose, and hydrolyzes it into fructose. The use of inulinases provides an alternative way to obtain fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and application of an inulinase from Pseudozima sp CCMB 300, Kluyveromyces marxianus (CCMB 322) and fungal endophytes (CCMB 328) isolated from Brazilian semi-arid region. The enzyme was characterized on the kinetic and stability nature and the results suggest that the isolated inulinase has potential for inulin hydrolysis reported rates of up to 100% conversion of inulin to fructose.
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spelling Assis, Sandra Aparecida de00766284506http://lattes.cnpq.br/3788862339761661Nascimento, Diego Sampaio2020-06-26T16:33:15Z2010-08-16NASCIMENTO, Diego Sampaio. Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose. 2010. 92 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.http://tede2.uefs.br:8080/handle/tede/1192Inulinases target the ?-2 linkage of inulin, a poliyfructan consisting of linear ?-2,1 linked fructose, and hydrolyzes it into fructose. The use of inulinases provides an alternative way to obtain fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and application of an inulinase from Pseudozima sp CCMB 300, Kluyveromyces marxianus (CCMB 322) and fungal endophytes (CCMB 328) isolated from Brazilian semi-arid region. The enzyme was characterized on the kinetic and stability nature and the results suggest that the isolated inulinase has potential for inulin hydrolysis reported rates of up to 100% conversion of inulin to fructose.A enzima inulinase hidrolisa a liga??o ?-2 da inulina, um pol?mero linear de frutose, formando xarope de frutose. O uso de inulinases constitui uma forma alternativa de obten??o deste produto. O objetivo deste trabalho foi avaliar a produ??o, realizar caracteriza??o e aplica??o de inulinases produzida por: Pseudozima sp CCMB 300, Kluyveromyces marxianus CCMB 322 e por um fungo endof?tico CCMB 328 isolados na regi?o semi-?rida do Brasil. A enzima foi caracterizada cineticamente a natureza da estabilidade t?rmica foi avaliada assim como o potencial de hidr?lise . Os resultados indicam que a inulinase isolada tem potencial para a hidr?lise de inulina apresentando taxas de convers?o de at? 100 % de inulina em frutose.Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2020-06-26T16:33:15Z No. of bitstreams: 1 dissertacao Diego Sampaio.pdf: 1042858 bytes, checksum: cef527c8051b309a25d01449bd8e4619 (MD5)Made available in DSpace on 2020-06-26T16:33:15Z (GMT). No. of bitstreams: 1 dissertacao Diego Sampaio.pdf: 1042858 bytes, checksum: cef527c8051b309a25d01449bd8e4619 (MD5) Previous issue date: 2010-08-16application/pdfporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASInulinainulinaselevedurassemi-?ridoBrasilInulininulinaseyeastssemi-aridBrazilCIENCIAS BIOLOGICASCIENCIAS BIOLOGICAS::BIOLOGIA GERALAplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutoseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-54735432730516527826006006006005026123383450589282-3439178843068202161-1634559385931244697info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALdissertacao Diego Sampaio.pdfdissertacao Diego Sampaio.pdfapplication/pdf1042858http://tede2.uefs.br:8080/bitstream/tede/1192/2/dissertacao+Diego+Sampaio.pdfcef527c8051b309a25d01449bd8e4619MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/1192/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/11922020-06-26 13:33:15.768oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2020-06-26T16:33:15Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false
dc.title.por.fl_str_mv Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
title Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
spellingShingle Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
Nascimento, Diego Sampaio
Inulina
inulinase
leveduras
semi-?rido
Brasil
Inulin
inulinase
yeasts
semi-arid
Brazil
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
title_full Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
title_fullStr Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
title_full_unstemmed Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
title_sort Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose
author Nascimento, Diego Sampaio
author_facet Nascimento, Diego Sampaio
author_role author
dc.contributor.advisor1.fl_str_mv Assis, Sandra Aparecida de
dc.contributor.authorID.fl_str_mv 00766284506
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3788862339761661
dc.contributor.author.fl_str_mv Nascimento, Diego Sampaio
contributor_str_mv Assis, Sandra Aparecida de
dc.subject.por.fl_str_mv Inulina
inulinase
leveduras
semi-?rido
Brasil
topic Inulina
inulinase
leveduras
semi-?rido
Brasil
Inulin
inulinase
yeasts
semi-arid
Brazil
CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Inulin
inulinase
yeasts
semi-arid
Brazil
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Inulinases target the ?-2 linkage of inulin, a poliyfructan consisting of linear ?-2,1 linked fructose, and hydrolyzes it into fructose. The use of inulinases provides an alternative way to obtain fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and application of an inulinase from Pseudozima sp CCMB 300, Kluyveromyces marxianus (CCMB 322) and fungal endophytes (CCMB 328) isolated from Brazilian semi-arid region. The enzyme was characterized on the kinetic and stability nature and the results suggest that the isolated inulinase has potential for inulin hydrolysis reported rates of up to 100% conversion of inulin to fructose.
publishDate 2010
dc.date.issued.fl_str_mv 2010-08-16
dc.date.accessioned.fl_str_mv 2020-06-26T16:33:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv NASCIMENTO, Diego Sampaio. Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose. 2010. 92 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/1192
identifier_str_mv NASCIMENTO, Diego Sampaio. Aplica??o de inulinases de leveduras isoladas do semi-?rido para a produ??o de concentrados de frutose. 2010. 92 f. Disserta??o (Mestrado Acad?mico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2010.
url http://tede2.uefs.br:8080/handle/tede/1192
dc.language.iso.fl_str_mv por
language por
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dc.relation.confidence.fl_str_mv 600
600
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dc.relation.department.fl_str_mv 5026123383450589282
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dc.publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
dc.publisher.program.fl_str_mv Mestrado Acad?mico em Biotecnologia
dc.publisher.initials.fl_str_mv UEFS
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
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