Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Bot?nica
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/996 |
Resumo: | Mulungu (Erythrina velutina Willd.) tree belongs to the family Leguminosae and shows high potential to family farming uses, medicinal and economic for northeast region of Brazil. However, the knowledge about the behavior of their seeds under stress condition is scarce. The objective of this study is to characterize the physiological and metabolic profiles of germination and to determine the activity of antioxidant enzymes in seeds and seedlings under conditions of heat, water and salt stress. Were assessed the percentage, average time, average speed, index of speed and coefficient of uniformity of germination, size and fresh and dry weight of seedlings. Cotyledons, embryonic axis, shoot, root, and cotyledons of quiescent seeds were collected and after extraction was subjected to measurements of total soluble sugars (TSS), reducing sugars (RS), soluble protein (TP), total amino acids (AA), proline and activities of catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GOPX) and glutathione S-transferase (GST). The germination, mobilization and degradation of reserves of E. velutina seeds were affected by low and high temperatures of incubation and also water restriction. Seed germination was not affected negatively by EC 18 dS.m-1, however water restriction promoted by NaCl solutions affected the growth of seedlings. Antioxidants activity varied depending on kind and level of applied stress, giving indications that germination might be slightly affected due to significant activity of antioxidant enzymes involved in defense against reactive oxygen species under conditions of abiotic stress. |
id |
UEFS_5afc0028e1bb38f0c373dd6851ff788e |
---|---|
oai_identifier_str |
oai:tede2.uefs.br:8080:tede/996 |
network_acronym_str |
UEFS |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
repository_id_str |
|
spelling |
Pelacani, Claudin?ia Regina78002796500http://lattes.cnpq.br/3792286346796868Reis, Renata Conduru Ribeiro2020-03-26T21:34:27Z2012-02-13REIS, Renata Conduru Ribeiro. Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga. 2012. 132 f. Tese (Doutorado Acad?mico em Bot?nica)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.http://tede2.uefs.br:8080/handle/tede/996Mulungu (Erythrina velutina Willd.) tree belongs to the family Leguminosae and shows high potential to family farming uses, medicinal and economic for northeast region of Brazil. However, the knowledge about the behavior of their seeds under stress condition is scarce. The objective of this study is to characterize the physiological and metabolic profiles of germination and to determine the activity of antioxidant enzymes in seeds and seedlings under conditions of heat, water and salt stress. Were assessed the percentage, average time, average speed, index of speed and coefficient of uniformity of germination, size and fresh and dry weight of seedlings. Cotyledons, embryonic axis, shoot, root, and cotyledons of quiescent seeds were collected and after extraction was subjected to measurements of total soluble sugars (TSS), reducing sugars (RS), soluble protein (TP), total amino acids (AA), proline and activities of catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GOPX) and glutathione S-transferase (GST). The germination, mobilization and degradation of reserves of E. velutina seeds were affected by low and high temperatures of incubation and also water restriction. Seed germination was not affected negatively by EC 18 dS.m-1, however water restriction promoted by NaCl solutions affected the growth of seedlings. Antioxidants activity varied depending on kind and level of applied stress, giving indications that germination might be slightly affected due to significant activity of antioxidant enzymes involved in defense against reactive oxygen species under conditions of abiotic stress.O mulungu (Erythrina velutina Willd.), pertence ? fam?lia Leguminosae e apresenta diversos usos, econ?mico, medicinal e para agricultura familiar nordestina. No entanto, pouco se conhece sobre o comportamento de suas sementes em condi??es de estresse, que ocorrem na regi?o nordeste. Objetivou-se com esse trabalho caracterizar os perfis fisiol?gico, metab?licos da germina??o e determinar a atividade de enzimas antioxidantes em sementes e pl?ntulas de mulungu sob condi??es de estresse t?rmico, h?drico e salino. Foram avaliados a porcentagem, ?ndice de velocidade, tempo m?dio, velocidade m?dia e coeficiente de uniformidade da germina??o, tamanho e massa fresca e seca de pl?ntulas. Os cotil?dones, eixo embrion?rio, parte a?rea, raiz, bem como os cotil?dones das sementes quiescentes foram separados e quantificados os teores de a??cares sol?veis totais (AST), a??cares redutores (AR), prote?nas sol?veis (PT), amino?cidos totais (AA), prolina e das atividades das enzimas catalase (CAT), ascorbato peroxidase (APX), guaiacol peroxidase (GOPX) e glutationa-S-transferase (GST). A germina??o, a mobiliza??o e degrada??o das reservas das sementes de E. velutina foram afetadas pelas temperaturas baixas e elevadas de incuba??o e tamb?m ? restri??o h?drica. A germina??o das sementes n?o foi afetada negativamente at? a CE de 18 dS.m-1, no entanto, a restri??o h?drica promovida pela solu??o de NaCl afetou negativamente o crescimento das pl?ntulas. A atividade antioxidante foi vari?vel dependendo do tipo e n?vel de estresse aplicado, no entanto forneceu indicativos de que a germina??o pode ser pouco afetada devido a atividade de enzimas envolvidas no mecanismo de defesa contra esp?cies reativas de oxig?nio sob condi??es de estresse abi?tico.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-03-26T21:34:27Z No. of bitstreams: 1 tese_Renata.pdf: 9753603 bytes, checksum: 683230d75d9a369f63cadb3ec2066eff (MD5)Made available in DSpace on 2020-03-26T21:34:27Z (GMT). No. of bitstreams: 1 tese_Renata.pdf: 9753603 bytes, checksum: 683230d75d9a369f63cadb3ec2066eff (MD5) Previous issue date: 2012-02-13application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em Bot?nicaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASSementesGermina??oEstresse abi?ticoMacromol?culasEnzimas antioxidantesErythrina velutinaSeedGerminationAbiotic stressMacromoleculesAntioxidant enzymesBOTANICA::TAXONOMIA VEGETALToler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatingainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-490822954372108619160060060050261233834505892821475540656322400511info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALtese_Renata.pdftese_Renata.pdfapplication/pdf9753603http://tede2.uefs.br:8080/bitstream/tede/996/2/tese_Renata.pdf683230d75d9a369f63cadb3ec2066effMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/996/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/9962020-03-26 18:34:27.132oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2020-03-26T21:34:27Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
title |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
spellingShingle |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga Reis, Renata Conduru Ribeiro Sementes Germina??o Estresse abi?tico Macromol?culas Enzimas antioxidantes Erythrina velutina Seed Germination Abiotic stress Macromolecules Antioxidant enzymes BOTANICA::TAXONOMIA VEGETAL |
title_short |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
title_full |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
title_fullStr |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
title_full_unstemmed |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
title_sort |
Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga |
author |
Reis, Renata Conduru Ribeiro |
author_facet |
Reis, Renata Conduru Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Pelacani, Claudin?ia Regina |
dc.contributor.authorID.fl_str_mv |
78002796500 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3792286346796868 |
dc.contributor.author.fl_str_mv |
Reis, Renata Conduru Ribeiro |
contributor_str_mv |
Pelacani, Claudin?ia Regina |
dc.subject.por.fl_str_mv |
Sementes Germina??o Estresse abi?tico Macromol?culas Enzimas antioxidantes Erythrina velutina |
topic |
Sementes Germina??o Estresse abi?tico Macromol?culas Enzimas antioxidantes Erythrina velutina Seed Germination Abiotic stress Macromolecules Antioxidant enzymes BOTANICA::TAXONOMIA VEGETAL |
dc.subject.eng.fl_str_mv |
Seed Germination Abiotic stress Macromolecules Antioxidant enzymes |
dc.subject.cnpq.fl_str_mv |
BOTANICA::TAXONOMIA VEGETAL |
description |
Mulungu (Erythrina velutina Willd.) tree belongs to the family Leguminosae and shows high potential to family farming uses, medicinal and economic for northeast region of Brazil. However, the knowledge about the behavior of their seeds under stress condition is scarce. The objective of this study is to characterize the physiological and metabolic profiles of germination and to determine the activity of antioxidant enzymes in seeds and seedlings under conditions of heat, water and salt stress. Were assessed the percentage, average time, average speed, index of speed and coefficient of uniformity of germination, size and fresh and dry weight of seedlings. Cotyledons, embryonic axis, shoot, root, and cotyledons of quiescent seeds were collected and after extraction was subjected to measurements of total soluble sugars (TSS), reducing sugars (RS), soluble protein (TP), total amino acids (AA), proline and activities of catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GOPX) and glutathione S-transferase (GST). The germination, mobilization and degradation of reserves of E. velutina seeds were affected by low and high temperatures of incubation and also water restriction. Seed germination was not affected negatively by EC 18 dS.m-1, however water restriction promoted by NaCl solutions affected the growth of seedlings. Antioxidants activity varied depending on kind and level of applied stress, giving indications that germination might be slightly affected due to significant activity of antioxidant enzymes involved in defense against reactive oxygen species under conditions of abiotic stress. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-13 |
dc.date.accessioned.fl_str_mv |
2020-03-26T21:34:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
REIS, Renata Conduru Ribeiro. Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga. 2012. 132 f. Tese (Doutorado Acad?mico em Bot?nica)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/996 |
identifier_str_mv |
REIS, Renata Conduru Ribeiro. Toler?ncia a estresses abi?ticos em sementes de Erythrina velutina Willd, (Leguminosae - Papilionoideae) nativa da caatinga. 2012. 132 f. Tese (Doutorado Acad?mico em Bot?nica)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
url |
http://tede2.uefs.br:8080/handle/tede/996 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-4908229543721086191 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
5026123383450589282 |
dc.relation.cnpq.fl_str_mv |
1475540656322400511 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acad?mico em Bot?nica |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
instname_str |
Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/996/2/tese_Renata.pdf http://tede2.uefs.br:8080/bitstream/tede/996/1/license.txt |
bitstream.checksum.fl_str_mv |
683230d75d9a369f63cadb3ec2066eff 7b5ba3d2445355f386edab96125d42b7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1796793640219049984 |