Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Mestrado Acad?mico em Recursos Gen?ticos Vegetais
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/907 |
Resumo: | Physic nut (Jatropha curcas L.) is a Euphorbiaceae of great economic interest for the production of biodiesel, but toxic components limit the use of its seeds. The aim of the study was to check the influence of water restriction in the electrophoretic profile of proteins during imbibition of seeds of physic nut. Two seeds lots from Cafarnaum/BA were characterized by analyzing germination percentage, weight of thousand seeds, biometry and moisture of seeds. The lot with higher germination percentage was imbibied water restriction in PEG 8000 solution (-0,8 MPa) and incubated at 25 ?C in continuous darkness for 0, 2, 4, 6, 8, 10, 12 and 14 days. Embryonic axis and endosperm were isolated, macerated in liquid nitrogen, added to phosphate buffer pH 7.5, centrifuged at 14.000 rpm at 4 ?C for 15 minutes for extraction of total proteins. The supernatant was collected for quantification of total protein using BSA as standard and performing absorbance reading at 595 nm in a spectrophotometer. Was carried the polyacrylamide gel electrophoresis at 300 V, 60 mA at 1:20 h to verify the protein profile in seed tissues. The proteins were visualized in the gel with coomassie blue solution. The electrophoretic profile differed between samples imbibed in water and in PEG 8000 (-0.8 MPa). It was possible to visualize protein bands related to plant defense to water restriction as well as an increased on intensity of bands corresponding to the molecular weight of the curcin in samples of endosperm of seeds imbibed under water restriction, when compared to dry seeds |
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spelling |
Castro, Renato Delmondez35940832172http://lattes.cnpq.br/094564880584412801125363525http://lattes.cnpq.br/7803642222552721Cerqueira, Marla Oliveira2019-10-14T22:22:43Z2013-05-08CERQUEIRA, Marla Oliveira. Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica. 2013. 75f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2013.http://tede2.uefs.br:8080/handle/tede/907Physic nut (Jatropha curcas L.) is a Euphorbiaceae of great economic interest for the production of biodiesel, but toxic components limit the use of its seeds. The aim of the study was to check the influence of water restriction in the electrophoretic profile of proteins during imbibition of seeds of physic nut. Two seeds lots from Cafarnaum/BA were characterized by analyzing germination percentage, weight of thousand seeds, biometry and moisture of seeds. The lot with higher germination percentage was imbibied water restriction in PEG 8000 solution (-0,8 MPa) and incubated at 25 ?C in continuous darkness for 0, 2, 4, 6, 8, 10, 12 and 14 days. Embryonic axis and endosperm were isolated, macerated in liquid nitrogen, added to phosphate buffer pH 7.5, centrifuged at 14.000 rpm at 4 ?C for 15 minutes for extraction of total proteins. The supernatant was collected for quantification of total protein using BSA as standard and performing absorbance reading at 595 nm in a spectrophotometer. Was carried the polyacrylamide gel electrophoresis at 300 V, 60 mA at 1:20 h to verify the protein profile in seed tissues. The proteins were visualized in the gel with coomassie blue solution. The electrophoretic profile differed between samples imbibed in water and in PEG 8000 (-0.8 MPa). It was possible to visualize protein bands related to plant defense to water restriction as well as an increased on intensity of bands corresponding to the molecular weight of the curcin in samples of endosperm of seeds imbibed under water restriction, when compared to dry seedsPinh?o-manso (Jatropha curcas L.) ? uma Euphorbiaceae de grande interesse para a produ??o do biodiesel, por?m componentes t?xicos limitam o uso de suas sementes. O trabalho objetivou verificar a influ?ncia da restri??o h?drica no perfil eletrofor?tico de prote?nas durante a embebi??o de sementes de pinh?o-manso. Foi realizada a caracteriza??o fisiol?gica de dois lotes de sementes oriundas de Cafarnaum/BA analisando a germina??o, peso de mil, biometria e umidade das sementes. O lote com maior germina??o foi submetido ? restri??o h?drica com PEG 8000 (-0,8 MPa), mantidas em B.O.D. a 25?C, sem fotoper?odo durante 0, 2, 4, 6, 8, 10, 12 e 14 dias. Eixo-embrion?rio e endosperma foram separados, macerados em nitrog?nio l?quido, adicionados a tamp?o fosfato pH 7,5, centrifugado a 14.000 rpm, 4? C por 15 minutos, para extra??o de prote?nas totais acumuladas nos compartimentos. O sobrenadante foi coletado para a quantifica??o destas prote?nas utilizando-se como padr?o, albumina bovina e leitura de absorb?ncia a 595 nm. Foi realizado eletroforese em gel de poliacrilamida a 300 V, 60 mA durante 1:20 h para verificar o perfil proteico dos compartimentos. As prote?nas foram identificadas no gel com solu??o de Azul de Comassie. O perfil eletrofor?tico diferiu nas amostras germinadas em ?gua e em PEG 8000 (-0,8 Mpa), o qual contribuiu para a visualiza??o de bandas proteicas relacionadas com a defesa da planta ? restri??o h?drica e aumento na intensidade das bandas correspondentes ao peso molecular da curcina nas amostras de endosperma das sementes submetidas ? restri??o h?drica em compara??o ? sementes secasSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2019-10-14T22:22:43Z No. of bitstreams: 1 Disserta??o_MARLA_EnviadaRGV.pdf: 1364376 bytes, checksum: 5e0408449b1f3923ccd3be9ef3e27747 (MD5)Made available in DSpace on 2019-10-14T22:22:43Z (GMT). No. of bitstreams: 1 Disserta??o_MARLA_EnviadaRGV.pdf: 1364376 bytes, checksum: 5e0408449b1f3923ccd3be9ef3e27747 (MD5) Previous issue date: 2013-05-08Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em Recursos Gen?ticos VegetaisUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASEndospermaeixo-embrion?riocomponentes t?xicosPEG 8000curcinaEndospermembryonic axistoxic componentsPEG 8000curcinGENETICA::GENETICA VEGETALPerfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?dricainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis81466464816222418746006006006005026123383450589282-73979202484192807163590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALDisserta??o_MARLA_EnviadaRGV.pdfDisserta??o_MARLA_EnviadaRGV.pdfapplication/pdf1364376http://tede2.uefs.br:8080/bitstream/tede/907/2/Disserta%C3%A7%C3%A3o_MARLA_EnviadaRGV.pdf5e0408449b1f3923ccd3be9ef3e27747MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/907/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/9072019-10-14 19:22:43.311oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-10-14T22:22:43Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
title |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
spellingShingle |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica Cerqueira, Marla Oliveira Endosperma eixo-embrion?rio componentes t?xicos PEG 8000 curcina Endosperm embryonic axis toxic components PEG 8000 curcin GENETICA::GENETICA VEGETAL |
title_short |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
title_full |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
title_fullStr |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
title_full_unstemmed |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
title_sort |
Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica |
author |
Cerqueira, Marla Oliveira |
author_facet |
Cerqueira, Marla Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Castro, Renato Delmondez |
dc.contributor.advisor1ID.fl_str_mv |
35940832172 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0945648805844128 |
dc.contributor.authorID.fl_str_mv |
01125363525 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7803642222552721 |
dc.contributor.author.fl_str_mv |
Cerqueira, Marla Oliveira |
contributor_str_mv |
Castro, Renato Delmondez |
dc.subject.por.fl_str_mv |
Endosperma eixo-embrion?rio componentes t?xicos PEG 8000 curcina |
topic |
Endosperma eixo-embrion?rio componentes t?xicos PEG 8000 curcina Endosperm embryonic axis toxic components PEG 8000 curcin GENETICA::GENETICA VEGETAL |
dc.subject.eng.fl_str_mv |
Endosperm embryonic axis toxic components PEG 8000 curcin |
dc.subject.cnpq.fl_str_mv |
GENETICA::GENETICA VEGETAL |
description |
Physic nut (Jatropha curcas L.) is a Euphorbiaceae of great economic interest for the production of biodiesel, but toxic components limit the use of its seeds. The aim of the study was to check the influence of water restriction in the electrophoretic profile of proteins during imbibition of seeds of physic nut. Two seeds lots from Cafarnaum/BA were characterized by analyzing germination percentage, weight of thousand seeds, biometry and moisture of seeds. The lot with higher germination percentage was imbibied water restriction in PEG 8000 solution (-0,8 MPa) and incubated at 25 ?C in continuous darkness for 0, 2, 4, 6, 8, 10, 12 and 14 days. Embryonic axis and endosperm were isolated, macerated in liquid nitrogen, added to phosphate buffer pH 7.5, centrifuged at 14.000 rpm at 4 ?C for 15 minutes for extraction of total proteins. The supernatant was collected for quantification of total protein using BSA as standard and performing absorbance reading at 595 nm in a spectrophotometer. Was carried the polyacrylamide gel electrophoresis at 300 V, 60 mA at 1:20 h to verify the protein profile in seed tissues. The proteins were visualized in the gel with coomassie blue solution. The electrophoretic profile differed between samples imbibed in water and in PEG 8000 (-0.8 MPa). It was possible to visualize protein bands related to plant defense to water restriction as well as an increased on intensity of bands corresponding to the molecular weight of the curcin in samples of endosperm of seeds imbibed under water restriction, when compared to dry seeds |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-05-08 |
dc.date.accessioned.fl_str_mv |
2019-10-14T22:22:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CERQUEIRA, Marla Oliveira. Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica. 2013. 75f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/907 |
identifier_str_mv |
CERQUEIRA, Marla Oliveira. Perfil eletrofor?tico de prote?nas em sementes de pinh?o-manso(Jatropha curcasl.) sob condi??es de restri??o h?drica. 2013. 75f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2013. |
url |
http://tede2.uefs.br:8080/handle/tede/907 |
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por |
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por |
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8146646481622241874 |
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-7397920248419280716 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Mestrado Acad?mico em Recursos Gen?ticos Vegetais |
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UEFS |
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Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
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Universidade Estadual de Feira de Santana |
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