Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae)
Ano de defesa: | 2016 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Recursos Gen?ticos Vegetais
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://localhost:8080/tede/handle/tede/468 |
Resumo: | (In vitro propagation and acclimatization of medicinal species: Alpinia zerumbet (Pers.) B.L.Burtt&R.M.Sm. (Zingiberaceae) and Solidago chilensis Meyen (Asteraceae). Alpinia zerumbet and Solidago chilensis are known for their ornamental and medicinal values. There are few reports in the literature on micropropagation of Alpinia zerumbet and none about Solidago chilensis, which demonstrates the need for studies. This work aimed to study in vitro propagation of the species, involving micropropagation and developing protocols for organogenesis. For in vitro establishment were tested different types of explants, disinfestation methods and antioxidants. For multiplication, these explants were tested with plant growth regulators naftalen acetic acid and benzilaminopurin isolated and combined, and dichlorophenoxyacetic acid and kinetin, isolated and combined. It was made anatomical characterization of callus formation. For acclimatization, after pre-acclimatization, the seedlings were transferred to plastic cups containing sterilized substrate, capped with plastic bottles and taken to a greenhouse with 50% shading, where the bottle caps were unscrewed slowly. The results showed success in establishing in vitro of A. zerumbet crucial step to start large-scale cultivation. Against contamination, the most effective treatment was 4ml PPM/L (Plant Preservative Mixture), controlling 100% of pathogens. As an antioxidant, ascorbic acid (2%) was the most efficient. There was budding A. zerumbet derivatives bud explants. There was no decline in the propagation rate during in vitro subcultures, however growth is very slow. S. chilensis, explants nodal segments showed a higher rate of multiplication. There was no decline in the spread rate over four subcultures in vitro and the seedlings had a high survival rate in the acclimatization phase. |
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Santana, Jos? Raniere Ferreira de5515544854986303635504http://lattes.cnpq.br/3870963809970283Rodrigues, Ana Carolina da Cunha2017-04-11T21:06:17Z2016-09-26RODRIGUES, Ana Carolina da Cunha. Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae). 2016. 95 f. Tese (Doutorado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016.http://localhost:8080/tede/handle/tede/468(In vitro propagation and acclimatization of medicinal species: Alpinia zerumbet (Pers.) B.L.Burtt&R.M.Sm. (Zingiberaceae) and Solidago chilensis Meyen (Asteraceae). Alpinia zerumbet and Solidago chilensis are known for their ornamental and medicinal values. There are few reports in the literature on micropropagation of Alpinia zerumbet and none about Solidago chilensis, which demonstrates the need for studies. This work aimed to study in vitro propagation of the species, involving micropropagation and developing protocols for organogenesis. For in vitro establishment were tested different types of explants, disinfestation methods and antioxidants. For multiplication, these explants were tested with plant growth regulators naftalen acetic acid and benzilaminopurin isolated and combined, and dichlorophenoxyacetic acid and kinetin, isolated and combined. It was made anatomical characterization of callus formation. For acclimatization, after pre-acclimatization, the seedlings were transferred to plastic cups containing sterilized substrate, capped with plastic bottles and taken to a greenhouse with 50% shading, where the bottle caps were unscrewed slowly. The results showed success in establishing in vitro of A. zerumbet crucial step to start large-scale cultivation. Against contamination, the most effective treatment was 4ml PPM/L (Plant Preservative Mixture), controlling 100% of pathogens. As an antioxidant, ascorbic acid (2%) was the most efficient. There was budding A. zerumbet derivatives bud explants. There was no decline in the propagation rate during in vitro subcultures, however growth is very slow. S. chilensis, explants nodal segments showed a higher rate of multiplication. There was no decline in the spread rate over four subcultures in vitro and the seedlings had a high survival rate in the acclimatization phase.Alpinia zerumbet e Solidago chilensis s?o conhecidas pelos valores ornamental e medicinal. Existem poucos relatos na literatura sobre micropropaga??o de Alpinia zerumbet e nenhum sobre Solidago chilensis, o que demonstra necessidade de estudos. Este trabalho objetivou estudar a propaga??o in vitro das esp?cies, envolvendo micropropaga??o e desenvolvendo protocolos para organog?nese. Para o estabelecimento in vitro foram testados diferentes tipos de explantes, m?todos de desinfesta??o e antioxidantes. Para multiplica??o, esses explantes foram testados com reguladores vegetais: ?cido naftalenoac?tico e benzilaminopurina isolados e combinados, al?m de ?cido 2,4-diclorofenoxiac?tico e cinetina, isolados e combinados. Foi feita caracteriza??o anat?mica da calog?nese. Para aclimatiza??o, ap?s a pr?-aclimatiza??o, as mudas foram transferidas para copos pl?sticos contendo substrato esterilizado, tampados com garrafas pet e levados para casa de vegeta??o com sombrite 50%, onde as tampas das garrafas foram desenroscadas aos poucos. Os resultados mostraram sucesso no estabelecimento in vitro de A. zerumbet, etapa crucial para iniciar cultivo em larga escala. Contra contamina??o, o tratamento mais efetivo foi 4mL de PPM/L (Plant Preservative Mixture), controlando 100% dos pat?genos. Como antioxidante, o ?cido asc?rbico (2%) foi o mais eficiente. Houve brota??o de A. zerumbet em explantes derivados de gemas. N?o foi observado decl?nio na taxa de propaga??o no decorrer dos subcultivos in vitro, contudo o crescimento ? muito lento. Para S. chilensis, explantes de segmentos nodais apresentaram maior taxa de multiplica??o. N?o foi observado decl?nio na taxa de propaga??o no decorrer de quatro subcultivos in vitro e as mudas apresentaram alto ?ndice de sobreviv?ncia na fase de aclimatiza??o.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-04-11T21:06:17Z No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5)Made available in DSpace on 2017-04-11T21:06:17Z (GMT). No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) Previous issue date: 2016-09-26application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em Recursos Gen?ticos VegetaisUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASArnicaCol?niaEmbriog?neseEstabelecimento in vitroOrganog?neseOxida??oEmbryogenesisIn vitro establishmentOrganogenesisOxidationBOTANICA::FISIOLOGIA VEGETALPropaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-32675240948481849496006006005026123383450589282-289993050269505198info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTese final Ana Carolina-.pdfTese final Ana Carolina-.pdfapplication/pdf2069305http://tede2.uefs.br:8080/bitstream/tede/468/2/Tese+final+Ana+Carolina-.pdfd639d8880ea9572c9a3ab7a9e940b690MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/468/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/4682017-04-11 18:06:17.165oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2017-04-11T21:06:17Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
title |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
spellingShingle |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) Rodrigues, Ana Carolina da Cunha Arnica Col?nia Embriog?nese Estabelecimento in vitro Organog?nese Oxida??o Embryogenesis In vitro establishment Organogenesis Oxidation BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
title_full |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
title_fullStr |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
title_full_unstemmed |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
title_sort |
Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae) |
author |
Rodrigues, Ana Carolina da Cunha |
author_facet |
Rodrigues, Ana Carolina da Cunha |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, Jos? Raniere Ferreira de |
dc.contributor.advisor1ID.fl_str_mv |
55155448549 |
dc.contributor.authorID.fl_str_mv |
86303635504 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3870963809970283 |
dc.contributor.author.fl_str_mv |
Rodrigues, Ana Carolina da Cunha |
contributor_str_mv |
Santana, Jos? Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Arnica Col?nia Embriog?nese Estabelecimento in vitro Organog?nese Oxida??o |
topic |
Arnica Col?nia Embriog?nese Estabelecimento in vitro Organog?nese Oxida??o Embryogenesis In vitro establishment Organogenesis Oxidation BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Embryogenesis In vitro establishment Organogenesis Oxidation |
dc.subject.cnpq.fl_str_mv |
BOTANICA::FISIOLOGIA VEGETAL |
description |
(In vitro propagation and acclimatization of medicinal species: Alpinia zerumbet (Pers.) B.L.Burtt&R.M.Sm. (Zingiberaceae) and Solidago chilensis Meyen (Asteraceae). Alpinia zerumbet and Solidago chilensis are known for their ornamental and medicinal values. There are few reports in the literature on micropropagation of Alpinia zerumbet and none about Solidago chilensis, which demonstrates the need for studies. This work aimed to study in vitro propagation of the species, involving micropropagation and developing protocols for organogenesis. For in vitro establishment were tested different types of explants, disinfestation methods and antioxidants. For multiplication, these explants were tested with plant growth regulators naftalen acetic acid and benzilaminopurin isolated and combined, and dichlorophenoxyacetic acid and kinetin, isolated and combined. It was made anatomical characterization of callus formation. For acclimatization, after pre-acclimatization, the seedlings were transferred to plastic cups containing sterilized substrate, capped with plastic bottles and taken to a greenhouse with 50% shading, where the bottle caps were unscrewed slowly. The results showed success in establishing in vitro of A. zerumbet crucial step to start large-scale cultivation. Against contamination, the most effective treatment was 4ml PPM/L (Plant Preservative Mixture), controlling 100% of pathogens. As an antioxidant, ascorbic acid (2%) was the most efficient. There was budding A. zerumbet derivatives bud explants. There was no decline in the propagation rate during in vitro subcultures, however growth is very slow. S. chilensis, explants nodal segments showed a higher rate of multiplication. There was no decline in the spread rate over four subcultures in vitro and the seedlings had a high survival rate in the acclimatization phase. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-09-26 |
dc.date.accessioned.fl_str_mv |
2017-04-11T21:06:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Ana Carolina da Cunha. Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae). 2016. 95 f. Tese (Doutorado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/468 |
identifier_str_mv |
RODRIGUES, Ana Carolina da Cunha. Propaga??o in vitro e aclimatiza??o de esp?cies medicinais: Alpinia zerumbet (Pers.) B. L. Burtt. & R. M. Sm. (Zingiberaceae) e Solidago chilensis Meyen (Asteraceae). 2016. 95 f. Tese (Doutorado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2016. |
url |
http://localhost:8080/tede/handle/tede/468 |
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por |
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por |
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600 600 600 |
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openAccess |
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Universidade Estadual de Feira de Santana |
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Doutorado Acad?mico em Recursos Gen?ticos Vegetais |
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DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
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Universidade Estadual de Feira de Santana |
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